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            "title": "Mixotrophic growth ofChlorella sorokiniana in outdoor enclosed photobioreactor",
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                    "creatorType": "author",
                    "firstName": "Yuan-Kun",
                    "lastName": "Lee"
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                    "creatorType": "author",
                    "firstName": "Sun-Yeun",
                    "lastName": "Ding"
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                    "firstName": "Chye-Hock",
                    "lastName": "Hoe"
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                    "firstName": "Chin-Seng",
                    "lastName": "Low"
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            "abstractNote": "Chlorella sorokiniana was cultured in heterotrophic or mixotrophic mode in outdoor enclosed tubular photobioreactor. The culture temperature was maintained at 32–35 °C. At night, theChlorella culture grew heterotrophically, and 0.1 M glucose was completely consumed. The biomass growth yield of glucose was 0.35 ± 0.001 g-biomass g-glucose−1. During the day, the algal culture grew mixotrophically and the biomass growth yield was 0.49 g-biomass g-glucose−1 in low density culture (initial biomass concentration, Xo = 2 g l−1), 0.56 g-biomass g-glucose−1 in medium density culture (Xo = 4 g l−1) and 0.46 g-biomass g-glucose−1 in high density culture (Xo = 7 g l−1). The daily area productivity of the culture, with Xo = 4 g l−1 corresponded to 127 g-biomass m−2 d−1 during the day and 79 g-biomass m−2 d−1 during the night. In all the cultures, the dissolved O2 concentration increased in the morning, reached the maximum value at noon, and then decreased in the afternoon. The dissolved CO2 concentration remained at 3 mBar in the morning and increased in the afternoon. Glycolate was not found to accumulate in culture medium.",
            "publicationTitle": "Journal of Applied Phycology",
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            "date": "March 29, 1996",
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            "pages": "163-169",
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            "DOI": "10.1007/BF02186320",
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            "title": "Microalgae cultivation in air-lift reactors: Modeling biomass yield and growth rate as a function of mixing frequency",
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                    "creatorType": "author",
                    "firstName": "Maria J.",
                    "lastName": "Barbosa"
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                    "firstName": "Johannes",
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                {
                    "creatorType": "author",
                    "firstName": "René H.",
                    "lastName": "Wijffels"
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            ],
            "abstractNote": "The slow development of microalgal biotechnology stems from the failure in the design of large-scale photobioreactors where light energy is efficiently utilized. Due to the light gradient inside the reactor and depending on the mixing properties, algae are subjected to certain light/dark cycles where the light period is characterized by a light gradient. These light/dark cycles will determine productivity and biomass yield on light energy. Air-lift reactors can be used for microalgae cultivation and medium-frequency light/dark cycles will be found in these systems. Light/dark cycles are associated with two basic parameters: first, the light fraction, i.e., the ratio between the light period and the cycle time and second, the frequency of the light/dark cycle. In the present work, light/dark cycles found in air-lift reactors were simulated taking into account the light gradient during the light period. The effect of medium-frequency cycle time (10-100 s) and light fraction (0.1-1) on growth rate and biomass yield on light energy of the microalgae Dunaliella tertiolecta was studied. The biomass yield and growth rates were mainly affected by the light fraction, while cycle time had little influence. Response surface methodology was used and a statistical model describing the effect of light fraction and cycle time on growth rate and biomass yield on light energy was developed. The use of the model as a reactor design criterion is discussed. © 2003 Wiley Periodicals, Inc. Biotechnol Bioeng 82: 170-179, 2003.",
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            "itemType": "journalArticle",
            "title": "Environmental Life Cycle Comparison of Algae to Other Bioenergy Feedstocks",
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                    "creatorType": "author",
                    "firstName": "Andres F.",
                    "lastName": "Clarens"
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                    "creatorType": "author",
                    "firstName": "Eleazer P.",
                    "lastName": "Resurreccion"
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                    "firstName": "Mark A.",
                    "lastName": "White"
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                    "firstName": "Lisa M.",
                    "lastName": "Colosi"
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            "abstractNote": "Algae are an attractive source of biomass energy since they do not compete with food crops and have higher energy yields per area than terrestrial crops. In spite of these advantages, algae cultivation has not yet been compared with conventional crops from a life cycle perspective. In this work, the impacts associated with algae production were determined using a stochastic life cycle model and compared with switchgrass, canola, and corn farming. The results indicate that these conventional crops have lower environmental impacts than algae in energy use, greenhouse gas emissions, and water regardless of cultivation location. Only in total land use and eutrophication potential do algae perform favorably. The large environmental footprint of algae cultivation is driven predominantly by upstream impacts, such as the demand for CO2 and fertilizer. To reduce these impacts, flue gas and, to a greater extent, wastewater could be used to offset most of the environmental burdens associated with algae. To demonstrate the benefits of algae production coupled with wastewater treatment, the model was expanded to include three different municipal wastewater effluents as sources of nitrogen and phosphorus. Each provided a significant reduction in the burdens of algae cultivation, and the use of source-separated urine was found to make algae more environmentally beneficial than the terrestrial crops.",
            "publicationTitle": "Environmental Science & Technology",
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            "DOI": "10.1021/es902838n",
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            "title": "An Empirical Model of the Phytoplankton Chrlorphyll: Carbon Ratio-the Conversion factor Between Productivity and Growth Rate",
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                    "creatorType": "author",
                    "firstName": "James E.",
                    "lastName": "Cloern"
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                    "firstName": "Christian",
                    "lastName": "Grenz"
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                    "firstName": "Lisa",
                    "lastName": "Vidergar-Lucas"
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            "abstractNote": "We present an empiricial model that describes the ratio of phytoplankton chlorophyll a to carbon, Chl: C, as a function of temperature, daily irradiance, and nutrient-limited growth rate. Our model is based on 219 published measurements of algal cultures exposed to light-limited or nutrient-limited growth conditions. We illustrate an approach for using this estimator of Chl: C to calculate phytoplankton population growth rate from measured primary productivity. This adaptive Chl:C model gives rise to interactive light-nutrient effects in which growth efficiency increases with nutrient availability under low-light conditions. One implication of this interaction is the enhancement of phytoplankton growth efficiency, in addition to enhancement of biomass yield, as a response to eutrophication.",
            "publicationTitle": "Limnology and Oceanography",
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            "date": "Nov., 1995",
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            "title": "Microalgal mass culture systems and methods: Their limitation and potential",
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                    "firstName": "Yuan-Kun",
                    "lastName": "Lee"
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            "abstractNote": "Cultivation of microalgae using natural and man-made open-ponds istechnologically simple, but not necessary cheap due to the high downstream processing cost. Products of microalgae cultured in open-pondscould only be marketed as value-added health food supplements, specialityfeed and reagents for research. The need to achieve higher productivityand to maintain monoculture of algae led to the development of enclosedtubular and flat plate photobioreactors. Despite higher biomassconcentration and better control of culture parameters, data accumulatedin the past 25 years have shown that the illuminated areal, volumetricproductivity and cost of production in these enclosed photobioreactors arenot better than those achievable in open-pond cultures. The technicaldifficulty in sterilizing these photobioreactors has hindered their applicationfor the production of high value pharmaceutical products. The alternativeof cultivating microalgae in heterotrophic mode in sterilizable fermentorshas achieved some commercial success. The maximum specific growth ratesof heterotrophic algal cultures are in general slower than those measured inphotosynthetic cultures. The biomass productivity of heterotrophic algalcultures has yet to achieve a level that is comparable to industrialproduction of yeast and other heterotrophic microrganisms. Mixotrophiccultivation of microalage takes advantage of their ability to utilise organicenergy and carbon substrates and perform photosynthesis concurrently. Moreover, production of some algal metabolites is light regulated. Futuredesign of sterilizable bioreactors for mixotrophic cultivation of microalgaemay have to consider the organic substrate the main source of energy andlight the supplemental source of energy, a change in mindset.",
            "publicationTitle": "Journal of Applied Phycology",
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            "date": "2001",
            "volume": "13",
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            "title": "Microalgae for biodiesel production and other applications: A review",
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                    "firstName": "Teresa M.",
                    "lastName": "Mata"
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            "abstractNote": "Sustainable production of renewable energy is being hotly debated globally since it is increasingly understood that first generation biofuels, primarily produced from food crops and mostly oil seeds are limited in their ability to achieve targets for biofuel production, climate change mitigation and economic growth. These concerns have increased the interest in developing second generation biofuels produced from non-food feedstocks such as microalgae, which potentially offer greatest opportunities in the longer term. This paper reviews the current status of microalgae use for biodiesel production, including their cultivation, harvesting, and processing. The microalgae species most used for biodiesel production are presented and their main advantages described in comparison with other available biodiesel feedstocks. The various aspects associated with the design of microalgae production units are described, giving an overview of the current state of development of algae cultivation systems (photo-bioreactors and open ponds). Other potential applications and products from microalgae are also presented such as for biological sequestration of CO2, wastewater treatment, in human health, as food additive, and for aquaculture.",
            "publicationTitle": "Renewable and Sustainable Energy Reviews",
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            "date": "January 2010",
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            "pages": "217-232",
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