[ { "key": "4HD9KHCU", "version": 214, "library": { "type": "group", "id": 62016, "name": "xavier.robin", "links": { "alternate": { "href": "https://www.zotero.org/groups/xavier.robin", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/62016/items/4HD9KHCU", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/xavier.robin/items/4HD9KHCU", "type": "text/html" } }, "meta": { "createdByUser": { "id": 12953, "username": "Calimo", "name": "Xavier Robin", "links": { "alternate": { "href": "https://www.zotero.org/calimo", "type": "text/html" } } }, "creatorSummary": "von Tobel et al.", "numChildren": 0 }, "data": { "key": "4HD9KHCU", "version": 214, "itemType": "journalArticle", "title": "Repeated exposure to Ochratoxin A generates a neuroinflammatory response, characterized by neurodegenerative M1 microglial phenotype", "creators": [ { "creatorType": "author", "firstName": "Jenny Sandström", "lastName": "von Tobel" }, { "creatorType": "author", "firstName": "Paola", "lastName": "Antinori" }, { "creatorType": "author", "firstName": "Marie-Gabrielle", "lastName": "Zurich" }, { "creatorType": "author", "firstName": "Robin", "lastName": "Rosset" }, { "creatorType": "author", "firstName": "Michael", "lastName": "Aschner" }, { "creatorType": "author", "firstName": "Florent", "lastName": "Glück" }, { "creatorType": "author", "firstName": "Alexander", "lastName": "Scherl" }, { "creatorType": "author", "firstName": "Florianne", "lastName": "Monnet-Tschudi" } ], "abstractNote": "Neurotoxic effects of the environmentally abundant mycotoxin Ochratoxin A (OTA) were studied in histotypic 3D rat brain cell cultures, comprising all brain cell types. Cultures were exposed to nanomolar OTA concentrations and samples were collected 48 h after a single exposure, or after 10 days of repeated administration. OTA-induced changes in gene- and protein expression, as well as alterations in cell morphology were assessed.\n\nForty-eight-hour OTA exposure resulted in a disruption of the neuronal cytoskeleton and reduced expression of several oligodendrocyte-specific markers indicative of demyelination. Astrocyte disturbances were revealed by a decrease in two astrocytic proteins involved in regulation of inflammatory responses, metallothioneins I and II. Repeated OTA administration induced a neuroinflammatory response, as visualized by an increase of isolectin B4 labelled cells, increased expression of pro-inflammatory cytokines, and detection of macrophagic ED1/CD68 positive cells, as well as an upregulation of neurodegenerative M1 microglial phenotype markers.\n\nPartial recovery from OTA-induced deleterious effects on oligodendrocytes and astrocytes was achieved by co-treatment with sonic hedgehog (SHH). In addition, metallothionein I and II co-treatment partially restored OTA-induced effects on oligodendrocytes after 48 h, and modulated microglial reactivity after 10 days. These results suggest that OTA-exposure affects Shh-signalling, which in turn may influence both oligodendrocytes and astrocytes. Furthermore, the primarily astrocytic proteins MTI/MTII may affect microglial activation. Thus the neuroinflammatory response appears to be downstream of OTA-induced effects on demyelination, axonal instabilities and astrocytes disturbances. In conclusion, repeated OTA-exposure induced a secondary neuroinflammatory response characterized by neurodegenerative M1 microglial activation and pro-inflammatory response that could exacerbate the neurodegenerative process.", "publicationTitle": "NeuroToxicology", "publisher": "", "place": "", "date": "", "volume": "", "issue": "", "section": "", "partNumber": "", "partTitle": "", "pages": "", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "NeuroToxicology", "DOI": "10.1016/j.neuro.2014.04.005", "citationKey": "", "url": "http://www.sciencedirect.com/science/article/pii/S0161813X14000643", "accessDate": "2014-06-09T08:03:51Z", "PMID": "", "PMCID": "", "ISSN": "0161-813X", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "", "libraryCatalog": "ScienceDirect", "callNumber": "", "rights": "", "extra": "", "tags": [ { "tag": "3D brain cell cultures", "type": 1 }, { "tag": "Demyelination", "type": 1 }, { "tag": "M1/M2 microglial phenotypes", "type": 1 }, { "tag": "Metallothionein I and II", "type": 1 }, { "tag": "Neuroinflammation", "type": 1 }, { "tag": "Ochratoxin A", "type": 1 }, { "tag": "Sonic hedgehog", "type": 1 } ], "collections": [ "PUMUZUWE", "TZNGZ92U" ], "relations": {}, "dateAdded": "2014-06-09T08:05:01Z", "dateModified": "2014-06-09T08:05:01Z" } }, { "key": "AXUWHWNJ", "version": 196, "library": { "type": "group", "id": 62016, "name": "xavier.robin", "links": { "alternate": { "href": "https://www.zotero.org/groups/xavier.robin", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/62016/items/AXUWHWNJ", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/xavier.robin/items/AXUWHWNJ", "type": "text/html" } }, "meta": { "createdByUser": { "id": 12953, "username": "Calimo", "name": "Xavier Robin", "links": { "alternate": { "href": "https://www.zotero.org/calimo", "type": "text/html" } } }, "creatorSummary": "Tiberti et al.", "parsedDate": "2013", "numChildren": 0 }, "data": { "key": "AXUWHWNJ", "version": 196, "itemType": "journalArticle", "title": "New biomarkers for stage determination in Trypanosoma brucei rhodesiense sleeping sickness patients", "creators": [ { "creatorType": "author", "firstName": "Natalia", "lastName": "Tiberti" }, { "creatorType": "author", "firstName": "Enock", "lastName": "Matovu" }, { "creatorType": "author", "firstName": "Alexandre", "lastName": "Hainard" }, { "creatorType": "author", "firstName": "John Charles", "lastName": "Enyaru" }, { "creatorType": "author", "firstName": "Veerle", "lastName": "Lejon" }, { "creatorType": "author", "firstName": "Xavier", "lastName": "Robin" }, { "creatorType": "author", "firstName": "Natacha", "lastName": "Turck" }, { "creatorType": "author", "firstName": "Dieudonné Mumba", "lastName": "Ngoyi" }, { "creatorType": "author", "firstName": "Sanjeev", "lastName": "Krishna" }, { "creatorType": "author", "firstName": "Sylvie", "lastName": "Bisser" } ], "abstractNote": "", "publicationTitle": "Clinical and translational medicine", "publisher": "", "place": "", "date": "2013", "volume": "2", "issue": "1", "section": "", "partNumber": "", "partTitle": "", "pages": "1–8", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "", "DOI": "", "citationKey": "", "url": "http://link.springer.com/article/10.1186/2001-1326-2-1", "accessDate": "2014-04-18T14:27:02Z", "PMID": "", "PMCID": "", "ISSN": "", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "", "libraryCatalog": "Google Scholar", "callNumber": "", "rights": "", "extra": "", "tags": [], "collections": [ "TZNGZ92U" ], "relations": {}, "dateAdded": "2014-04-18T14:28:33Z", "dateModified": "2014-04-18T14:28:33Z" } }, { "key": "Q6TT6CQE", "version": 196, "library": { "type": "group", "id": 62016, "name": "xavier.robin", "links": { "alternate": { "href": "https://www.zotero.org/groups/xavier.robin", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/62016/items/Q6TT6CQE", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/xavier.robin/items/Q6TT6CQE", "type": "text/html" } }, "meta": { "createdByUser": { "id": 12953, "username": "Calimo", "name": "Xavier Robin", "links": { "alternate": { "href": "https://www.zotero.org/calimo", "type": "text/html" } } }, "creatorSummary": "Lejon et al.", "parsedDate": "2013", "numChildren": 0 }, "data": { "key": "Q6TT6CQE", "version": 196, "itemType": "journalArticle", "title": "Human African trypanosomiasis", "creators": [ { "creatorType": "author", "firstName": "VEERLE", "lastName": "Lejon" }, { "creatorType": "author", "firstName": "M.", "lastName": "Bentivoglio" }, { "creatorType": "author", "firstName": "JOSE RAMON", "lastName": "Franco" } ], "abstractNote": "", "publicationTitle": "Neuroparasitology and Tropical Neurology: Handbook of Clinical Neurology Series (Editors: Aminoff, Boller, Swaab)", "publisher": "", "place": "", "date": "2013", "volume": "114", "issue": "", "section": "", "partNumber": "", "partTitle": "", "pages": "169", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "", "DOI": "", "citationKey": "", "url": "http://www.google.com/books?hl=en&lr=&id=bJx3g30aDhIC&oi=fnd&pg=PA169&ots=T5pFkkv1RH&sig=1PX0Wq6MyGbDBY0nPqZRFT1pKIs", "accessDate": "2014-04-18T14:25:49Z", "PMID": "", "PMCID": "", "ISSN": "", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "", "libraryCatalog": "Google Scholar", "callNumber": "", "rights": "", "extra": "", "tags": [], "collections": [ "TZNGZ92U" ], "relations": {}, "dateAdded": "2014-04-18T14:28:33Z", "dateModified": "2014-04-18T14:28:33Z" } }, { "key": "5M3CP2DK", "version": 168, "library": { "type": "group", "id": 62016, "name": "xavier.robin", "links": { "alternate": { "href": "https://www.zotero.org/groups/xavier.robin", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/62016/items/5M3CP2DK", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/xavier.robin/items/5M3CP2DK", "type": "text/html" } }, "meta": { "createdByUser": { "id": 12953, "username": "Calimo", "name": "Xavier Robin", "links": { "alternate": { "href": "https://www.zotero.org/calimo", "type": "text/html" } } }, "creatorSummary": "Craft et al.", "parsedDate": "2013", "numChildren": 0 }, "data": { "key": "5M3CP2DK", "version": 168, "itemType": "journalArticle", "title": "Recent advances in quantitative neuroproteomics", "creators": [ { "creatorType": "author", "firstName": "George E.", "lastName": "Craft" }, { "creatorType": "author", "firstName": "Anshu", "lastName": "Chen" }, { "creatorType": "author", "firstName": "Angus C.", "lastName": "Nairn" } ], "abstractNote": "The field of proteomics is undergoing rapid development in a number of different areas including improvements in mass spectrometric platforms, peptide identification algorithms and bioinformatics. In particular, new and/or improved approaches have established robust methods that not only allow for in-depth and accurate peptide and protein identification and modification, but also allow for sensitive measurement of relative or absolute quantitation. These methods are beginning to be applied to the area of neuroproteomics, but the central nervous system poses many specific challenges in terms of quantitative proteomics, given the large number of different neuronal cell types that are intermixed and that exhibit distinct patterns of gene and protein expression. This review highlights the recent advances that have been made in quantitative neuroproteomics, with a focus on work published over the last five years that applies emerging methods to normal brain function as well as to various neuropsychiatric disorders including schizophrenia and drug addiction as well as of neurodegenerative diseases including Parkinson’s disease and Alzheimer’s disease. While older methods such as two-dimensional polyacrylamide electrophoresis continued to be used, a variety of more in-depth MS-based approaches including both label (ICAT, iTRAQ, TMT, SILAC, SILAM), label-free (label-free, MRM, SWATH) and absolute quantification methods, are rapidly being applied to neurobiological investigations of normal and diseased brain tissue as well as of cerebrospinal fluid (CSF). While the biological implications of many of these studies remain to be clearly established, that there is a clear need for standardization of experimental design and data analysis, and that the analysis of protein changes in specific neuronal cell types in the central nervous system remains a serious challenge, it appears that the quality and depth of the more recent quantitative proteomics studies is beginning to shed light on a number of aspects of neuroscience that relates to normal brain function as well as of the changes in protein expression and regulation that occurs in neuropsychiatric and neurodegenerative disorders.", "publicationTitle": "Methods", "publisher": "", "place": "", "date": "juin 15, 2013", "volume": "61", "issue": "3", "section": "", "partNumber": "", "partTitle": "", "pages": "186-218", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "Methods", "DOI": "10.1016/j.ymeth.2013.04.008", "citationKey": "", "url": "http://www.sciencedirect.com/science/article/pii/S1046202313001126", "accessDate": "2014-02-28T18:52:39Z", "PMID": "", "PMCID": "", "ISSN": "1046-2023", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "", "libraryCatalog": "ScienceDirect", "callNumber": "", "rights": "", "extra": "", "tags": [ { "tag": "Label-free quantitation", "type": 1 }, { "tag": "Mass Spectrometry", "type": 1 }, { "tag": "Neuropeptidomics", "type": 1 }, { "tag": "Proteomics", "type": 1 }, { "tag": "Quantitative neuroproteomics", "type": 1 }, { "tag": "Two-dimensional gel electrophoresis", "type": 1 }, { "tag": "isobaric labeling", "type": 1 } ], "collections": [ "TZNGZ92U" ], "relations": {}, "dateAdded": "2014-02-28T18:53:40Z", "dateModified": "2014-02-28T18:53:40Z" } }, { "key": "E2TQ8U4E", "version": 163, "library": { "type": "group", "id": 62016, "name": "xavier.robin", "links": { "alternate": { "href": "https://www.zotero.org/groups/xavier.robin", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/62016/items/E2TQ8U4E", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/xavier.robin/items/E2TQ8U4E", "type": "text/html" } }, "meta": { "createdByUser": { "id": 12953, "username": "Calimo", "name": "Xavier Robin", "links": { "alternate": { "href": "https://www.zotero.org/calimo", "type": "text/html" } } }, "creatorSummary": "Salvisberg et al.", "parsedDate": "2014", "numChildren": 0 }, "data": { "key": "E2TQ8U4E", "version": 163, "itemType": "journalArticle", "title": "Exploring the human tear fluid: discovery of new biomarkers in multiple sclerosis", "creators": [ { "creatorType": "author", "firstName": "Cindy", "lastName": "Salvisberg" }, { "creatorType": "author", "firstName": "Nadja", "lastName": "Tajouri" }, { "creatorType": "author", "firstName": "Alexandre", "lastName": "Hainard" }, { "creatorType": "author", "firstName": "Pierre R.", "lastName": "Burkhard" }, { "creatorType": "author", "firstName": "Patrice H.", "lastName": "Lalive" }, { "creatorType": "author", "firstName": "Natacha", "lastName": "Turck" } ], "abstractNote": "Purpose Multiple sclerosis is the first cause of progressive neurological disability among young adults living in Western countries. Its diagnosis is mostly based on clinical evaluation, neuroimaging and in some cases cerebrospinal fluid analysis but no definitive diagnostic test exists. We proposed here that the exploration of tears from multiple sclerosis patients could lead to the discovery of new biomarkers. Experimental design Thirty multiple sclerosis patients (20% men) recruited to the Geneva University Hospitals were included in our study (mean age ± SD (years): 42.4 ± 15.9). Twenty five control patients (32% men) were also enrolled (mean age ± SD (years): 42.7 ± 15.1). Tears, cerebrospinal fluid (CSF) or blood was collected for each patient. Three independent quantitative (tandem mass tag, TMT) experiments were carried out between tears from multiple sclerosis and control patients. Protein verification was performed by western blot on tears and CSF and by ELISA on serum samples. Results Combined proteomics analyses provided 185 identified tear proteins. Among the differential proteins, alpha-1 antichymotrypsin was the only one to be significantly increased in the 3 experiments with similar ratios (ratios 1.6 to 2.5, p<0.05). Its tear, CSF and serum elevation were further confirmed by western blot and ELISA, respectively. Conclusions and clinical relevance This study supports the concept that modifications of the tear proteome can reflect biological abnormalities associated with multiple sclerosis and perhaps other inflammatory conditions affecting the CNS. In addition, alpha-1-antichymotrypsin elevation in tear fluid emerges as a promising biomarker for the diagnosis of multiple sclerosis. This article is protected by copyright. All rights reserved", "publicationTitle": "PROTEOMICS – Clinical Applications", "publisher": "", "place": "", "date": "2014", "volume": "", "issue": "", "section": "", "partNumber": "", "partTitle": "", "pages": "n/a–n/a", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "", "DOI": "10.1002/prca.201300053", "citationKey": "", "url": "http://onlinelibrary.wiley.com/doi/10.1002/prca.201300053/abstract", "accessDate": "2014-02-12T17:39:58Z", "PMID": "", "PMCID": "", "ISSN": "1862-8354", "archive": "", "archiveLocation": "", "shortTitle": "Exploring the human tear fluid", "language": "en", "libraryCatalog": "Wiley Online Library", "callNumber": "", "rights": "This article is protected by copyright. All rights reserved", "extra": "", "tags": [ { "tag": "Biomarkers", "type": 1 }, { "tag": "Proteomics", "type": 1 }, { "tag": "isobaric labeling", "type": 1 }, { "tag": "multiple sclerosis", "type": 1 }, { "tag": "tears", "type": 1 } ], "collections": [ "PUMUZUWE", "TZNGZ92U" ], "relations": {}, "dateAdded": "2014-02-12T21:04:07Z", "dateModified": "2014-02-12T21:04:07Z" } }, { "key": "DZ27NEZ3", "version": 173, "library": { "type": "group", "id": 62016, "name": "xavier.robin", "links": { "alternate": { "href": "https://www.zotero.org/groups/xavier.robin", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/62016/items/DZ27NEZ3", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/xavier.robin/items/DZ27NEZ3", "type": "text/html" } }, "meta": { "createdByUser": { "id": 12953, "username": "Calimo", "name": "Xavier Robin", "links": { "alternate": { "href": "https://www.zotero.org/calimo", "type": "text/html" } } }, "creatorSummary": "Nyawira Maranga et al.", "parsedDate": "2013-09-30", "numChildren": 0 }, "data": { "key": "DZ27NEZ3", "version": 173, "itemType": "journalArticle", "title": "IL-6 is Upregulated in Late-Stage Disease in Monkeys Experimentally Infected with Trypanosoma brucei rhodesiense", "creators": [ { "creatorType": "author", "firstName": "Dawn", "lastName": "Nyawira Maranga" }, { "creatorType": "author", "firstName": "John Maina", "lastName": "Kagira" }, { "creatorType": "author", "firstName": "Christopher Kariuki", "lastName": "Kinyanjui" }, { "creatorType": "author", "firstName": "Simon", "lastName": "Muturi Karanja" }, { "creatorType": "author", "firstName": "Naomi", "lastName": "Wangari Maina" }, { "creatorType": "author", "firstName": "Maina", "lastName": "Ngotho" } ], "abstractNote": "The management of human African trypanosomiasis (HAT) is constrained by lack of simple-to-use diagnostic, staging, and treatment tools. The search for novel biomarkers is, therefore, essential in the fight against HAT. The current study aimed at investigating the potential of IL-6 as an adjunct parameter for HAT stage determination in vervet monkey model. Four adult vervet monkeys (Chlorocebus aethiops) were experimentally infected with Trypanosoma brucei rhodesiense and treated subcuratively at 28 days after infection (dpi) to induce late stage disease. Three noninfected monkeys formed the control group. Cerebrospinal fluid (CSF) and blood samples were obtained at weekly intervals and assessed for various biological parameters. A typical HAT-like infection was observed. The late stage was characterized by significant () elevation of CSF IL-6, white blood cell count, and total protein starting 35 dpi with peak levels of these parameters coinciding with relapse parasitaemia. Brain immunohistochemical staining revealed an increase in brain glial fibrillary acidic protein expression indicative of reactive astrogliosis in infected animals which were euthanized in late-stage disease. The elevation of IL-6 in CSF which accompanied other HAT biomarkers indicates onset of parasite neuroinvasion and show potential for use as an adjunct late-stage disease biomarker in the Rhodesian sleeping sickness.", "publicationTitle": "Clinical and Developmental Immunology", "publisher": "", "place": "", "date": "2013/09/30", "volume": "2013", "issue": "", "section": "", "partNumber": "", "partTitle": "", "pages": "", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "", "DOI": "10.1155/2013/320509", "citationKey": "", "url": "http://www.hindawi.com/journals/cdi/2013/320509/abs/", "accessDate": "2013-11-24T11:02:26Z", "PMID": "", "PMCID": "", "ISSN": "1740-2522", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "en", "libraryCatalog": "www.hindawi.com", "callNumber": "", "rights": "", "extra": "", "tags": [], "collections": [ "8QFQVCUA", "E6BR434W", "TZNGZ92U", "XRTS6Q6R" ], "relations": {}, "dateAdded": "2013-11-24T11:04:09Z", "dateModified": "2013-11-24T11:04:43Z" } }, { "key": "XIHVAMTN", "version": 173, "library": { "type": "group", "id": 62016, "name": "xavier.robin", "links": { "alternate": { "href": "https://www.zotero.org/groups/xavier.robin", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/62016/items/XIHVAMTN", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/xavier.robin/items/XIHVAMTN", "type": "text/html" } }, "meta": { "createdByUser": { "id": 12953, "username": "Calimo", "name": "Xavier Robin", "links": { "alternate": { "href": "https://www.zotero.org/calimo", "type": "text/html" } } }, "creatorSummary": "Holzmuller et al.", "parsedDate": "2013", "numChildren": 0 }, "data": { "key": "XIHVAMTN", "version": 173, "itemType": "journalArticle", "title": "Proteomics: a new way to improve human African trypanosomiasis diagnosis?", "creators": [ { "creatorType": "author", "firstName": "Philippe", "lastName": "Holzmuller" }, { "creatorType": "author", "firstName": "Pascal", "lastName": "Grébaut" }, { "creatorType": "author", "firstName": "Silla", "lastName": "Semballa" }, { "creatorType": "author", "firstName": "Mary Isabel", "lastName": "Gonzatti" }, { "creatorType": "author", "firstName": "Anne", "lastName": "Geiger" } ], "abstractNote": "", "publicationTitle": "Expert Review of Proteomics", "publisher": "", "place": "", "date": "06/2013", "volume": "10", "issue": "3", "section": "", "partNumber": "", "partTitle": "", "pages": "289-301", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "", "DOI": "10.1586/epr.13.14", "citationKey": "", "url": "http://www.expert-reviews.com/doi/abs/10.1586/epr.13.14", "accessDate": "2013-11-24T11:02:56Z", "PMID": "", "PMCID": "", "ISSN": "1478-9450, 1744-8387", "archive": "", "archiveLocation": "", "shortTitle": "Proteomics", "language": "", "libraryCatalog": "CrossRef", "callNumber": "", "rights": "", "extra": "", "tags": [], "collections": [ "4G5P8GBH", "8QFQVCUA", "E6BR434W", "TZNGZ92U", "XRTS6Q6R" ], "relations": {}, "dateAdded": "2013-11-24T11:04:09Z", "dateModified": "2013-11-24T11:04:09Z" } }, { "key": "HNBGQQ5D", "version": 173, "library": { "type": "group", "id": 62016, "name": "xavier.robin", "links": { "alternate": { "href": "https://www.zotero.org/groups/xavier.robin", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/62016/items/HNBGQQ5D", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/xavier.robin/items/HNBGQQ5D", "type": "text/html" } }, "meta": { "createdByUser": { "id": 12953, "username": "Calimo", "name": "Xavier Robin", "links": { "alternate": { "href": "https://www.zotero.org/calimo", "type": "text/html" } } }, "creatorSummary": "Holzmuller et al.", "parsedDate": "2014-01-01", "numChildren": 0 }, "data": { "key": "HNBGQQ5D", "version": 173, "itemType": "bookSection", "title": "Proteomics and African Trypanosomes: Shedding New Light on Host–Vector–Parasite Interactions and Impact on Control Methods", "creators": [ { "creatorType": "author", "firstName": "Philippe", "lastName": "Holzmuller" }, { "creatorType": "author", "firstName": "Pascal", "lastName": "Grébaut" }, { "creatorType": "author", "firstName": "Anne", "lastName": "Geiger" }, { "creatorType": "editor", "firstName": "Stefan", "lastName": "Magez" }, { "creatorType": "editor", "firstName": "Magdalena", "lastName": "Radwanska" } ], "abstractNote": "Most mammalian and vector host species have acquired strategies by selective pressure to mislead the trypanosome and to win the fight during their molecular dialogue. Due to the same evolutionary pressure, trypanosomes have acquired strategies to bypass the host defences and to ensure the completion of their complex life cycles. Elucidation of these complex molecular crosstalks will improve the understanding of trypanosomes’ variability with respect to virulence and pathogenicity, will help to define trypansome-specific host biomarkers and will help to refine control strategies for African trypanosomoses. Advances in proteomics applications have provided new insights on African trypanosomes and on the biochemical interactions with their tsetse vectors and mammalian hosts. In this chapter, we present the interest of proteomics to characterise trypanosomes–hosts interactions, a synthetic review of proteomics studies performed on the parasite and its respective hosts, a discussion on the contributions and pitfalls of using diverse proteomics tools, a view for future prospects on proteomics dedicated to African trypanosomes and a projection of new conceptual approaches (i.e. metabolomics, interactomics, population proteomics) to accurately decipher insect vector–trypanosome–mammalian host interactions, with the idea of further developing new tools to improve trypanosomoses control.", "bookTitle": "Trypanosomes and Trypanosomiasis", "series": "", "seriesNumber": "", "volume": "", "numberOfVolumes": "", "edition": "", "date": "2014/01/01", "publisher": "Springer Vienna", "place": "", "originalDate": "", "originalPublisher": "", "originalPlace": "", "format": "", "pages": "161-187", "ISBN": "978-3-7091-1555-8, 978-3-7091-1556-5", "DOI": "", "citationKey": "", "url": "http://link.springer.com/chapter/10.1007/978-3-7091-1556-5_7", "accessDate": "2013-11-24T11:02:43Z", "ISSN": "", "archive": "", "archiveLocation": "", "shortTitle": "Proteomics and African Trypanosomes", "language": "en", "libraryCatalog": "link.springer.com", "callNumber": "", "rights": "©2014 Springer-Verlag Wien", "extra": "", "tags": [ { "tag": "Infectious Diseases", "type": 1 }, { "tag": "Medical Microbiology", "type": 1 }, { "tag": "Parasitology", "type": 1 } ], "collections": [ "4G5P8GBH", "8QFQVCUA", "E6BR434W", "K2HIHR6Q", "TZNGZ92U", "XRTS6Q6R" ], "relations": {}, "dateAdded": "2013-11-24T11:04:09Z", "dateModified": "2013-11-24T11:04:09Z" } }, { "key": "CERQG3CW", "version": 65, "library": { "type": "group", "id": 62016, "name": "xavier.robin", "links": { "alternate": { "href": "https://www.zotero.org/groups/xavier.robin", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/62016/items/CERQG3CW", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/xavier.robin/items/CERQG3CW", "type": "text/html" } }, "meta": { "createdByUser": { "id": 12953, "username": "Calimo", "name": "Xavier Robin", "links": { "alternate": { "href": "https://www.zotero.org/calimo", "type": "text/html" } } }, "creatorSummary": "Liu et al.", "parsedDate": "2013", "numChildren": 0 }, "data": { "key": "CERQG3CW", "version": 65, "itemType": "journalArticle", "title": "Serum Biomarkers Reveal Long-term Cardiac Injury in Isoproterenol-treated African Green Monkeys", "creators": [ { "creatorType": "author", "firstName": "Yashu", "lastName": "Liu" }, { "creatorType": "author", "firstName": "Toufan", "lastName": "Parman" }, { "creatorType": "author", "firstName": "Bridget", "lastName": "Schneider" }, { "creatorType": "author", "firstName": "Benben", "lastName": "Song" }, { "creatorType": "author", "firstName": "Amit K.", "lastName": "Galande" }, { "creatorType": "author", "firstName": "Dave", "lastName": "Anderson" }, { "creatorType": "author", "firstName": "Jon", "lastName": "Mirsalis" } ], "abstractNote": "The assessment of cardiac toxicity is a major challenge in both drug development and clinical trials, and numerous marketed pharmaceuticals have been removed from the market due to unpredicted cardiac effects. Serum troponins are widely used indicators of cardiac injury; however, they are short-lived and have not been validated in preclinical animal models. In this study, we have used filter-aided sample preparation (FASP) and tandem mass tag (TMT) labeling to investigate serum protein alterations in isoproterenol-treated African green monkeys. Our results showed that the combination of FASP and TMT labeling provided highly reproducible and efficient sample preparation, which enables us to identify and quantify serum proteins with high confidence. We focused on the proteins that exhibit long-term alteration upon isoproterenol injection and discovered nine proteins exhibiting significant changes at 48 and 72 h postdosing. We further chose three proteins, serum amyloid A (SAA), frutose biphosphate aldolase A (FBAA), and fetuin A, for validation using enzyme-linked immunosorbent assay (ELISA). The serum concentration of SAA showed a ?50 fold increase, while concentration of FBAA and fetuin A exhibited a significant decrease accompanying isoproterenol-induced cardiotoxicity. This work provides valuable insights for multimarker evaluation of long-term cardiac injury.", "publicationTitle": "Journal of Proteome Research", "publisher": "", "place": "", "date": "avril 5, 2013", "volume": "12", "issue": "4", "section": "", "partNumber": "", "partTitle": "", "pages": "1830-1837", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "J. Proteome Res.", "DOI": "10.1021/pr3011809", "citationKey": "", "url": "http://dx.doi.org/10.1021/pr3011809", "accessDate": "2013-06-08T16:21:40Z", "PMID": "", "PMCID": "", "ISSN": "1535-3893", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "", "libraryCatalog": "ACS Publications", "callNumber": "", "rights": "", "extra": "", "tags": [], "collections": [ "TZNGZ92U" ], "relations": {}, "dateAdded": "2013-06-08T16:22:06Z", "dateModified": "2013-06-08T16:22:06Z" } }, { "key": "QNBIB3VZ", "version": 68, "library": { "type": "group", "id": 62016, "name": "xavier.robin", "links": { "alternate": { "href": "https://www.zotero.org/groups/xavier.robin", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/62016/items/QNBIB3VZ", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/xavier.robin/items/QNBIB3VZ", "type": "text/html" } }, "meta": { "createdByUser": { "id": 12953, "username": "Calimo", "name": "Xavier Robin", "links": { "alternate": { "href": "https://www.zotero.org/calimo", "type": "text/html" } } }, "creatorSummary": "Tiberti et al.", "parsedDate": "2013", "numChildren": 0 }, "data": { "key": "QNBIB3VZ", "version": 68, "itemType": "journalArticle", "title": "Neopterin Is a Cerebrospinal Fluid Marker for Treatment Outcome Evaluation in Patients Affected by Trypanosoma brucei gambiense Sleeping Sickness", "creators": [ { "creatorType": "author", "firstName": "Natalia", "lastName": "Tiberti" }, { "creatorType": "author", "firstName": "Veerle", "lastName": "Lejon" }, { "creatorType": "author", "firstName": "Alexandre", "lastName": "Hainard" }, { "creatorType": "author", "firstName": "Bertrand", "lastName": "Courtioux" }, { "creatorType": "author", "firstName": "Xavier", "lastName": "Robin" }, { "creatorType": "author", "firstName": "Natacha", "lastName": "Turck" }, { "creatorType": "author", "firstName": "Krister", "lastName": "Kristensson" }, { "creatorType": "author", "firstName": "Enock", "lastName": "Matovu" }, { "creatorType": "author", "firstName": "John Charles", "lastName": "Enyaru" }, { "creatorType": "author", "firstName": "Dieudonné", "lastName": "Mumba Ngoyi" }, { "creatorType": "author", "firstName": "Sanjeev", "lastName": "Krishna" }, { "creatorType": "author", "firstName": "Sylvie", "lastName": "Bisser" }, { "creatorType": "author", "firstName": "Joseph Mathu", "lastName": "Ndung′u" }, { "creatorType": "author", "firstName": "Philippe", "lastName": "Büscher" }, { "creatorType": "author", "firstName": "Jean-Charles", "lastName": "Sanchez" } ], "abstractNote": "Author SummaryThe reduction of the number of lumbar punctures performed during the follow-up of patients affected by sleeping sickness (HAT) is considered a research priority. Follow-up, consisting of the examination of cerebrospinal fluid (CSF) for presence of parasites and for the number of leukocytes, is necessary to assess treatment outcome. However, diagnosis of treatment failure is still imperfect and WHO encourages improvements in defining criteria. Many studies have attempted to standardize actual methods and to define a cut-off for the number of white blood cells in CSF to define relapses, while only few have proposed alternatives to current practice. Here we show that neopterin, already proven to be a powerful marker for staging T. b. gambiense HAT, is also useful in evaluating post-therapeutic outcome. The measurement of neopterin concentration in CSF during the follow-up may allow reduction in the number of lumbar punctures from five to three for the majority of cured patients.", "publicationTitle": "PLOS Neglected Tropical Diseases", "publisher": "", "place": "", "date": "février 28, 2013", "volume": "7", "issue": "2", "section": "", "partNumber": "", "partTitle": "", "pages": "e2088", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "PLoS Negl Trop Dis", "DOI": "10.1371/journal.pntd.0002088", "citationKey": "", "url": "http://dx.doi.org/10.1371/journal.pntd.0002088", "accessDate": "2013-03-09T14:42:49Z", "PMID": "", "PMCID": "", "ISSN": "", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "", "libraryCatalog": "PLoS Negl Trop Dis", "callNumber": "", "rights": "", "extra": "PMID: 23469311", "tags": [], "collections": [ "4G5P8GBH", "75VPBAXC", "E6BR434W", "NPRXC88X", "TZNGZ92U", "XRTS6Q6R" ], "relations": {}, "dateAdded": "2013-03-09T14:43:34Z", "dateModified": "2013-03-09T14:52:32Z" } }, { "key": "EVC8QKFB", "version": 1, "library": { "type": "group", "id": 62016, "name": "xavier.robin", "links": { "alternate": { "href": "https://www.zotero.org/groups/xavier.robin", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/62016/items/EVC8QKFB", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/xavier.robin/items/EVC8QKFB", "type": "text/html" } }, "meta": { "createdByUser": { "id": 12953, "username": "Calimo", "name": "Xavier Robin", "links": { "alternate": { "href": "https://www.zotero.org/calimo", "type": "text/html" } } }, "creatorSummary": "Jakoby et al.", "parsedDate": "2012", "numChildren": 0 }, "data": { "key": "EVC8QKFB", "version": 1, "itemType": "journalArticle", "title": "Improved reporter ion assignment of raw isobaric stable isotope labeled liquid chromatography/matrix-assisted laser desorption/ionization tandem time-of-flight mass spectral data for quantitative proteomics", "creators": [ { "creatorType": "author", "firstName": "Thomas", "lastName": "Jakoby" }, { "creatorType": "author", "firstName": "Andreas", "lastName": "Tholey" }, { "creatorType": "author", "firstName": "Bart H.J.", "lastName": "van den Berg" } ], "abstractNote": "RATIONALE Isobaric labeling strategies (e.g. iTRAQ or TMT) are commonly applied in tandem mass spectrometric (MS/MS) level quantitative proteomics. However, we frequently observed missing isotope reporter ion signals in a large-scale liquid chromatography/matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometric (LC/MALDI-TOF/TOF) quantitative proteomics experiment. To understand this issue, we systematically investigated the processing of MS/MS spectra into peak lists prior to peptide identification and quantification. METHODS A 15-protein standard, with six proteins in different concentrations, was labeled with iTRAQ 4-plex, iTRAQ 8-plex or TMT 6-plex, tryptic digested and measured using LC/MALDI-TOF/TOF. Three commercially and open-source available peak list generation software tools were compared based on missing reporter ions, peptide identification and quantification. RESULTS We found that each tool discarded lower-intensity reporter ions, when they followed a higher intensity reporter ion, due to the implemented de-isotoping algorithms. By using the non-de-isotoping setting within TS2Mascot, we found that all reporter ions are exported, yet less peptides were identified with Mascot. Therefore, we developed a strategy merging the de-isotoped and non-de-isotoped outputs from TS2Mascot using the Perl script RICmerge.pl. CONCLUSIONS With this approach, we correctly quantified all labeled peptides that were identified within the 15-protein standard. This strategy allows improved annotation of isobaric tag labeled peptide MS/MS spectra and improves downstream peptide and protein quantification in proteomics studies. Copyright © 2012 John Wiley & Sons, Ltd.", "publicationTitle": "Rapid Communications in Mass Spectrometry", "publisher": "", "place": "", "date": "2012", "volume": "26", "issue": "23", "section": "", "partNumber": "", "partTitle": "", "pages": "2777–2785", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "", "DOI": "10.1002/rcm.6403", "citationKey": "", "url": "http://onlinelibrary.wiley.com/doi/10.1002/rcm.6403/abstract", "accessDate": "2012-11-05T08:22:52Z", "PMID": "", "PMCID": "", "ISSN": "1097-0231", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "en", "libraryCatalog": "Wiley Online Library", "callNumber": "", "rights": "Copyright © 2012 John Wiley & Sons, Ltd.", "extra": "", "tags": [], "collections": [ "TZNGZ92U" ], "relations": {}, "dateAdded": "2012-11-05T08:23:01Z", "dateModified": "2012-11-05T08:23:01Z" } }, { "key": "6Z5P9Z2R", "version": 1, "library": { "type": "group", "id": 62016, "name": "xavier.robin", "links": { "alternate": { "href": "https://www.zotero.org/groups/xavier.robin", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/62016/items/6Z5P9Z2R", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/xavier.robin/items/6Z5P9Z2R", "type": "text/html" } }, "meta": { "createdByUser": { "id": 12953, "username": "Calimo", "name": "Xavier Robin", "links": { "alternate": { "href": "https://www.zotero.org/calimo", "type": "text/html" } } }, "creatorSummary": "Khoontawad et al.", "parsedDate": "2012-09-18", "numChildren": 0 }, "data": { "key": "6Z5P9Z2R", "version": 1, "itemType": "journalArticle", "title": "Proteomic Identification of Plasma Protein Tyrosine Phosphatase Alpha and Fibronectin Associated with Liver Fluke, Opisthorchis viverrini, Infection", "creators": [ { "creatorType": "author", "firstName": "Jarinya", "lastName": "Khoontawad" }, { "creatorType": "author", "firstName": "Umawadee", "lastName": "Laothong" }, { "creatorType": "author", "firstName": "Sittiruk", "lastName": "Roytrakul" }, { "creatorType": "author", "firstName": "Porntip", "lastName": "Pinlaor" }, { "creatorType": "author", "firstName": "Jason", "lastName": "Mulvenna" }, { "creatorType": "author", "firstName": "Chaisiri", "lastName": "Wongkham" }, { "creatorType": "author", "firstName": "Puangrat", "lastName": "Yongvanit" }, { "creatorType": "author", "firstName": "Chawalit", "lastName": "Pairojkul" }, { "creatorType": "author", "firstName": "Eimorn", "lastName": "Mairiang" }, { "creatorType": "author", "firstName": "Paiboon", "lastName": "Sithithaworn" }, { "creatorType": "author", "firstName": "Somchai", "lastName": "Pinlaor" } ], "abstractNote": "Opisthorchiasis caused by Opisthorchis viverrini induces periductal fibrosis via host immune/inflammatory responses. Plasma protein alteration during host-parasite interaction-mediated inflammation may provide potential diagnostic and/or prognostic biomarkers. To search for target protein changes in O. viverrini-infected hamsters, a 1-D PAGE gel band was trypsin-digested and analyzed by a LC-MS/MS-based proteomics approach in the plasma profile of infected hamsters, and applied to humans. Sixty seven proteins were selected for further analysis based on at least two unique tryptic peptides with protein ID score >10 and increased expression at least two times across time points. These proteins have not been previously identified in O. viverrini-associated infection. Among those, proteins involved in structural (19%), immune response (13%), cell cycle (10%) and transcription (10%) were highly expressed. Western blots revealed an expression level of protein tyrosine phosphatase alpha (PTPα) which reached a peak at 1 month and subsequently tended to decrease. Fibronectin significantly increased at 1 month and tended to increase with time, supporting proteomic analysis. PTPα was expressed in the cytoplasm of inflammatory cells, while fibronectin was observed mainly in the cytoplasm of fibroblasts and the extracellular matrix at periductal fibrosis areas. In addition, these protein levels significantly increased in the plasma of O. viverrini-infected patients compared to healthy individuals, and significantly decreased at 2-months post-treatment, indicating their potential as disease markers. In conclusion, our results suggest that plasma PTPα and fibronectin may be associated with opisthorchiasis and the hamster model provides the basis for development of novel diagnostic markers in the future.", "publicationTitle": "PLoS ONE", "publisher": "", "place": "", "date": "septembre 18, 2012", "volume": "7", "issue": "9", "section": "", "partNumber": "", "partTitle": "", "pages": "e45460", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "PLoS ONE", "DOI": "10.1371/journal.pone.0045460", "citationKey": "", "url": "http://dx.doi.org/10.1371/journal.pone.0045460", "accessDate": "2012-09-27T07:04:48Z", "PMID": "", "PMCID": "", "ISSN": "", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "", "libraryCatalog": "PLoS ONE", "callNumber": "", "rights": "", "extra": "", "tags": [], "collections": [ "TZNGZ92U" ], "relations": {}, "dateAdded": "2012-09-27T07:05:39Z", "dateModified": "2012-09-27T07:05:39Z" } }, { "key": "BP8DS85G", "version": 1, "library": { "type": "group", "id": 62016, "name": "xavier.robin", "links": { "alternate": { "href": "https://www.zotero.org/groups/xavier.robin", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/62016/items/BP8DS85G", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/xavier.robin/items/BP8DS85G", "type": "text/html" } }, "meta": { "createdByUser": { "id": 12953, "username": "Calimo", "name": "Xavier Robin", "links": { "alternate": { "href": "https://www.zotero.org/calimo", "type": "text/html" } } }, "creatorSummary": "Brown", "parsedDate": "2012", "numChildren": 0 }, "data": { "key": "BP8DS85G", "version": 1, "itemType": "journalArticle", "title": "Osteopontin: A key link between immunity, inflammation and the central nervous system", "creators": [ { "creatorType": "author", "firstName": "Amanda", "lastName": "Brown" } ], "abstractNote": "Osteopontin (OPN) is a pro-inflammatory cytokine that can be secreted from many cells including activated macrophages and T-lymphocytes. Elevated levels of osteopontin in the plasma, cerebrospinal fluid or brain of individuals with neurodegenerative diseases such as multiple sclerosis (MS), Parkinson’s and Alzheimer’s disease and more recently in HIV-associated neurocognitive disorder has been reported. However, except for the case of MS, little is known regarding the molecular mechanisms by which OPN may exacerbate disease. Alternatively, OPN through its ability to promote cell survival may in some contexts function in the brain in a protective capacity. OPN has several protein motifs that allow it to engage with several different signaling pathways involved in immunity and inflammation. A better understanding of the cellular pathways that are regulated by OPN in cells of the central nervous system is required to uncover its putative role in neuronal homeostasis.", "publicationTitle": "Translational Neuroscience", "publisher": "", "place": "", "date": "2012", "volume": "3", "issue": "3", "section": "", "partNumber": "", "partTitle": "", "pages": "288-293", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "", "DOI": "10.2478/s13380-012-0028-7", "citationKey": "", "url": "http://www.springerlink.com/content/d6x1v23382585u44/abstract/", "accessDate": "2012-09-04T07:25:20Z", "PMID": "", "PMCID": "", "ISSN": "2081-3856", "archive": "", "archiveLocation": "", "shortTitle": "Osteopontin", "language": "", "libraryCatalog": "SpringerLink", "callNumber": "", "rights": "", "extra": "", "tags": [ { "tag": "Medicine", "type": 1 } ], "collections": [ "TZNGZ92U" ], "relations": {}, "dateAdded": "2012-09-04T07:25:49Z", "dateModified": "2012-09-04T07:25:49Z" } }, { "key": "7TZZXDPP", "version": 1, "library": { "type": "group", "id": 62016, "name": "xavier.robin", "links": { "alternate": { "href": "https://www.zotero.org/groups/xavier.robin", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/62016/items/7TZZXDPP", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/xavier.robin/items/7TZZXDPP", "type": "text/html" } }, "meta": { "createdByUser": { "id": 12953, "username": "Calimo", "name": "Xavier Robin", "links": { "alternate": { "href": "https://www.zotero.org/calimo", "type": "text/html" } } }, "creatorSummary": "Tiberti et al.", "parsedDate": "2012", "numChildren": 0 }, "data": { "key": "7TZZXDPP", "version": 1, "itemType": "journalArticle", "title": "Cerebrospinal Fluid Neopterin as Marker of the Meningo-Encephalitic Stage of Trypanosoma brucei gambiense Sleeping Sickness", "creators": [ { "creatorType": "author", "firstName": "Natalia", "lastName": "Tiberti" }, { "creatorType": "author", "firstName": "Alexandre", "lastName": "Hainard" }, { "creatorType": "author", "firstName": "Veerle", "lastName": "Lejon" }, { "creatorType": "author", "firstName": "Bertrand", "lastName": "Courtioux" }, { "creatorType": "author", "firstName": "Enock", "lastName": "Matovu" }, { "creatorType": "author", "firstName": "John Charles", "lastName": "Enyaru" }, { "creatorType": "author", "firstName": "Xavier", "lastName": "Robin" }, { "creatorType": "author", "firstName": "Natacha", "lastName": "Turck" }, { "creatorType": "author", "firstName": "Krister", "lastName": "Kristensson" }, { "creatorType": "author", "firstName": "Dieudonné Mumba", "lastName": "Ngoyi" }, { "creatorType": "author", "firstName": "Gedeão M. L.", "lastName": "Vatunga" }, { "creatorType": "author", "firstName": "Sanjeev", "lastName": "Krishna" }, { "creatorType": "author", "firstName": "Philippe", "lastName": "Büscher" }, { "creatorType": "author", "firstName": "Sylvie", "lastName": "Bisser" }, { "creatorType": "author", "firstName": "Joseph Mathu", "lastName": "Ndung’u" }, { "creatorType": "author", "firstName": "Jean-Charles", "lastName": "Sanchez" } ], "abstractNote": "BackgroundSleeping sickness, or human African trypanosomiasis (HAT), is a protozoan disease that affects rural communities in sub-Saharan Africa. Determination of the disease stage, essential for correct treatment, represents a key issue in the management of patients. In the present study we evaluated the potential of CXCL10, CXCL13, ICAM-1, VCAM-1, MMP-9, B2MG, neopterin and IgM to complement current methods for staging Trypanosoma brucei gambiense patients.Methods and FindingsFive hundred and twelve T. b. gambiense HAT patients originated from Angola, Chad and the Democratic Republic of the Congo (D.R.C.). Their classification as stage 2 (S2) was based on the number of white blood cells (WBC) (>5/µL) or presence of parasites in the cerebrospinal fluid (CSF). The CSF concentration of the eight markers was first measured on a training cohort encompassing 100 patients (44 S1 and 56 S2). IgM and neopterin were the best in discriminating between the two stages of disease with 86.4% and 84.1% specificity respectively, at 100% sensitivity. When a validation cohort (412 patients) was tested, neopterin (14.3 nmol/L) correctly classified 88% of S1 and S2 patients, confirming its high staging power. On this second cohort, neopterin also predicted both the presence of parasites, and of neurological signs, with the same ability as IgM and WBC, the current reference for staging.ConclusionsThis study has demonstrated that neopterin is an excellent biomarker for staging T. b. gambiense HAT patients. A rapid diagnostic test for detecting this metabolite in CSF could help in more accurate stage determination.", "publicationTitle": "PLoS ONE", "publisher": "", "place": "", "date": "juillet 18, 2012", "volume": "7", "issue": "7", "section": "", "partNumber": "", "partTitle": "", "pages": "e40909", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "PLoS ONE", "DOI": "10.1371/journal.pone.0040909", "citationKey": "", "url": "http://dx.doi.org/10.1371/journal.pone.0040909", "accessDate": "2012-07-23T06:55:58Z", "PMID": "", "PMCID": "", "ISSN": "", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "", "libraryCatalog": "PLoS ONE", "callNumber": "", "rights": "", "extra": "", "tags": [], "collections": [ "4G5P8GBH", "75VPBAXC", "E6BR434W", "NPRXC88X", "TZNGZ92U" ], "relations": {}, "dateAdded": "2012-07-23T07:00:59Z", "dateModified": "2012-07-23T07:00:59Z" } }, { "key": "NXTQH9S2", "version": 1, "library": { "type": "group", "id": 62016, "name": "xavier.robin", "links": { "alternate": { "href": "https://www.zotero.org/groups/xavier.robin", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/62016/items/NXTQH9S2", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/xavier.robin/items/NXTQH9S2", "type": "text/html" } }, "meta": { "createdByUser": { "id": 12953, "username": "Calimo", "name": "Xavier Robin", "links": { "alternate": { "href": "https://www.zotero.org/calimo", "type": "text/html" } } }, "creatorSummary": "Jakoby et al.", "parsedDate": "2012", "numChildren": 0 }, "data": { "key": "NXTQH9S2", "version": 1, "itemType": "journalArticle", "title": "Quantitative Protease Cleavage Site Profiling using Tandem-Mass-Tag Labeling and LC–MALDI-TOF/TOF MS/MS Analysis", "creators": [ { "creatorType": "author", "firstName": "Thomas", "lastName": "Jakoby" }, { "creatorType": "author", "firstName": "Bart HJ", "lastName": "van den Berg" }, { "creatorType": "author", "firstName": "Andreas", "lastName": "Tholey" } ], "abstractNote": "Knowledge of cleavage site specificity and activity are major prerequisites for understanding protease function. On the basis of a recently presented approach for proteomic identification of cleavage sites (PICS) in proteome-derived peptide libraries, we developed an isobaric labeling quantitative LC?MALDI-TOF/TOF MS/MS approach (Q-PICS) for simultaneous determination of cleavage site specificity and robust relative quantification of proteolytic events. For GluC-protease, 737 cleavage sites were identified in a yeast proteome-derived peptide library; 94.0% showed the typical GluC specificity for peptide bonds at glutamyl and aspartyl residues. The six-plex tandem mass tagging strategy allowed for three simultaneous replicates in a single run, guaranteeing high confidence and robust statistics for quantitative measurements. Using the quantitative capacity of Q-PICS, we performed a comparison of cleavage site specificity of GluC in two different buffer systems. The results support earlier findings describing that apparent difference between the buffer systems are probably caused by the inhibitory effect of bicarbonate on the overall GluC activity and that the preference for Glu-X bonds compared to Asp-X bonds is independent of the buffer system used.\nKnowledge of cleavage site specificity and activity are major prerequisites for understanding protease function. On the basis of a recently presented approach for proteomic identification of cleavage sites (PICS) in proteome-derived peptide libraries, we developed an isobaric labeling quantitative LC?MALDI-TOF/TOF MS/MS approach (Q-PICS) for simultaneous determination of cleavage site specificity and robust relative quantification of proteolytic events. For GluC-protease, 737 cleavage sites were identified in a yeast proteome-derived peptide library; 94.0% showed the typical GluC specificity for peptide bonds at glutamyl and aspartyl residues. The six-plex tandem mass tagging strategy allowed for three simultaneous replicates in a single run, guaranteeing high confidence and robust statistics for quantitative measurements. Using the quantitative capacity of Q-PICS, we performed a comparison of cleavage site specificity of GluC in two different buffer systems. The results support earlier findings describing that apparent difference between the buffer systems are probably caused by the inhibitory effect of bicarbonate on the overall GluC activity and that the preference for Glu-X bonds compared to Asp-X bonds is independent of the buffer system used.", "publicationTitle": "Journal of Proteome Research", "publisher": "", "place": "", "date": "2012", "volume": "11", "issue": "3", "section": "", "partNumber": "", "partTitle": "", "pages": "1812-1820", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "", "DOI": "10.1021/pr201051e", "citationKey": "", "url": "http://dx.doi.org/10.1021/pr201051e", "accessDate": "", "PMID": "", "PMCID": "", "ISSN": "1535-3893", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "", "libraryCatalog": "ACS Publications", "callNumber": "", "rights": "", "extra": "", "tags": [], "collections": [ "TZNGZ92U" ], "relations": {}, "dateAdded": "2012-03-30T11:20:08Z", "dateModified": "2012-04-10T07:25:42Z" } }, { "key": "EFTFJF7E", "version": 1, "library": { "type": "group", "id": 62016, "name": "xavier.robin", "links": { "alternate": { "href": "https://www.zotero.org/groups/xavier.robin", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/62016/items/EFTFJF7E", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/xavier.robin/items/EFTFJF7E", "type": "text/html" } }, "meta": { "createdByUser": { "id": 12953, "username": "Calimo", "name": "Xavier Robin", "links": { "alternate": { "href": "https://www.zotero.org/calimo", "type": "text/html" } } }, "creatorSummary": "Matovu et al.", "parsedDate": "2012", "numChildren": 0 }, "data": { "key": "EFTFJF7E", "version": 1, "itemType": "journalArticle", "title": "Towards Point-of-Care Diagnostic and Staging Tools for Human African Trypanosomiaisis", "creators": [ { "creatorType": "author", "firstName": "Enock", "lastName": "Matovu" }, { "creatorType": "author", "firstName": "Anne Juliet", "lastName": "Kazibwe" }, { "creatorType": "author", "firstName": "Claire Mack", "lastName": "Mugasa" }, { "creatorType": "author", "firstName": "Joseph Mathu", "lastName": "Ndungu" }, { "creatorType": "author", "firstName": "Zablon Kithingi", "lastName": "Njiru" } ], "abstractNote": "Human African trypanosomiasis is a debilitating disease prevalent in rural sub-Saharan Africa. Control of this disease almost exclusively relies on chemotherapy that should be driven by accurate diagnosis, given the unacceptable toxicity of the few available drugs. Unfortunately, the available diagnostics are characterised by low sensitivities due to the inherent low parasitaemia in natural infections. Demonstration of the trypanosomes in body fluids, which is a prerequisite before treatment, often follows complex algorithms. In this paper, we review the available diagnostics and explore recent advances towards development of novel point-of-care diagnostic tests.", "publicationTitle": "Journal of Tropical Medicine", "publisher": "", "place": "", "date": "2012", "volume": "2012", "issue": "", "section": "", "partNumber": "", "partTitle": "", "pages": "1-9", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "", "DOI": "10.1155/2012/340538", "citationKey": "", "url": "http://www.hindawi.com/journals/jtm/2012/340538/", "accessDate": "2012-04-02T07:05:11Z", "PMID": "", "PMCID": "", "ISSN": "1687-9686, 1687-9694", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "", "libraryCatalog": "CrossRef", "callNumber": "", "rights": "", "extra": "", "tags": [], "collections": [ "4G5P8GBH", "E6BR434W", "TZNGZ92U" ], "relations": {}, "dateAdded": "2012-04-02T07:08:10Z", "dateModified": "2012-04-02T07:08:10Z" } }, { "key": "ZBRVWART", "version": 1, "library": { "type": "group", "id": 62016, "name": "xavier.robin", "links": { "alternate": { "href": "https://www.zotero.org/groups/xavier.robin", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/62016/items/ZBRVWART", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/xavier.robin/items/ZBRVWART", "type": "text/html" } }, "meta": { "createdByUser": { "id": 12953, "username": "Calimo", "name": "Xavier Robin", "links": { "alternate": { "href": "https://www.zotero.org/calimo", "type": "text/html" } } }, "creatorSummary": "Sanchez-Quiles et al.", "parsedDate": "2011", "numChildren": 0 }, "data": { "key": "ZBRVWART", "version": 1, "itemType": "journalArticle", "title": "HSV-1 Cgal+ infection promotes quaking RNA binding protein production and induces nuclear-cytoplasmic shuttling of quaking I-5 isoform in human hepatoma cells", "creators": [ { "creatorType": "author", "firstName": "virginia", "lastName": "Sanchez-Quiles" }, { "creatorType": "author", "firstName": "Maria I.", "lastName": "Mora" }, { "creatorType": "author", "firstName": "Victor", "lastName": "Segura" }, { "creatorType": "author", "firstName": "Anna", "lastName": "Greco" }, { "creatorType": "author", "firstName": "Alberto L", "lastName": "Epstein" }, { "creatorType": "author", "firstName": "Maria Giovanna", "lastName": "Foschini" }, { "creatorType": "author", "firstName": "Loic", "lastName": "Dayon" }, { "creatorType": "author", "firstName": "Jean-Charles", "lastName": "Sanchez" }, { "creatorType": "author", "firstName": "Jesus", "lastName": "Prieto" }, { "creatorType": "author", "firstName": "Fernando J.", "lastName": "Corrales" }, { "creatorType": "author", "firstName": "Enrique", "lastName": "Santamaria" } ], "abstractNote": "Herpes virus type 1 (HSV-1) based oncolytic vectors arise as a promising therapeutic alternative for neoplastic diseases including hepatocellular carcinoma (HCC). However, the mechanisms mediating the host cell response to such treatments are not completely known. It is well established that HSV-1 infection induces functional and structural alterations in the nucleus of the host cell. In the present work, we have used gel-based and shotgun proteomic strategies to elucidate the signalling pathways impaired in the nucleus of human hepatoma cells (Huh7) upon HSV-1 Cgal+ infection. Both approaches allowed the identification of differential proteins suggesting impairment of cell functions involved in many aspects of host-virus interaction such as transcription regulation, mRNA processing, and mRNA splicing. Based on our proteomic data and additional functional studies, cellular protein quaking content (QKI) increases four hours post-infection (hpi), when viral immediate-early genes such as ICP4 and ICP27 could be also detected. Depletion of QKI expression by small interfering RNA (siRNA) results in reduction of viral immediate-early protein levels, subsequent decrease in early and late viral protein content, and a reduction in the viral yield indicating that QKI directly interferes with viral replication. In particular, HSV-1 Cgal+ induces a transient increase in quaking I-5 isoform (QKI-5) levels, in parallel with an enhancement of p27Kip1 protein content. Moreover, immunofluorescence microscopy showed an early nuclear redistribution of QKI-5, shuttling from the nucleus to the cytosol and co-localizing with nectin-1 in cell to cell contact regions at 16-24 hpi. These evidences shade new light to mechanisms mediating hepatoma cell response to HSV-1 vectors highlighting QKI as a central molecular mediator.", "publicationTitle": "Molecular & Cellular Proteomics", "publisher": "", "place": "", "date": "avril 05 , 2011", "volume": "", "issue": "", "section": "", "partNumber": "", "partTitle": "", "pages": "", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "", "DOI": "10.1074/mcp.M111.009126", "citationKey": "", "url": "http://www.mcponline.org/content/early/2011/04/05/mcp.M111.009126.abstract", "accessDate": "2011-06-14T13:20:10Z", "PMID": "", "PMCID": "", "ISSN": "", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "", "libraryCatalog": "Highwire 2.0", "callNumber": "", "rights": "", "extra": "", "tags": [], "collections": [ "TZNGZ92U" ], "relations": {}, "dateAdded": "2011-12-05T09:45:09Z", "dateModified": "2011-12-05T09:45:09Z" } }, { "key": "83GU5SQ7", "version": 1, "library": { "type": "group", "id": 62016, "name": "xavier.robin", "links": { "alternate": { "href": "https://www.zotero.org/groups/xavier.robin", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/62016/items/83GU5SQ7", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/xavier.robin/items/83GU5SQ7", "type": "text/html" } }, "meta": { "createdByUser": { "id": 12953, "username": "Calimo", "name": "Xavier Robin", "links": { "alternate": { "href": "https://www.zotero.org/calimo", "type": "text/html" } } }, "creatorSummary": "Grady et al.", "parsedDate": "2011-02-08", "numChildren": 0 }, "data": { "key": "83GU5SQ7", "version": 1, "itemType": "journalArticle", "title": "Population Vulnerability and Disability in Kenya's Tsetse Fly Habitats", "creators": [ { "creatorType": "author", "firstName": "Sue C.", "lastName": "Grady" }, { "creatorType": "author", "firstName": "Joseph P.", "lastName": "Messina" }, { "creatorType": "author", "firstName": "Paul F.", "lastName": "McCord" } ], "abstractNote": "The tsetse fly's influence on human health occurs through direct and indirect exposure pathways. Directly, the fly is a vector for the disease human African trypanosomiasis (HAT), which it spreads to nearly 18,000 new victims each year. Indirectly, the fly is a vector for African Animal Trypanosomaisis (AAT) also known as nagana, which restricts agricultural production, limiting the availability of food and contributing to impoverished conditions across rural sub-Saharan Africa. This historical study used 1999 census data to determine the prevalence of disability among residents and migrants living within Kenya's 7 tsetse fly belts. The results showed that the HAT transmission cycle may differ for residents and migrants with mechanisms leading to exposures that are environmentally driven for residents and economically driven for migrants. The combined burdens of HAT and AAT and the opportunity costs of agricultural production in AAT areas are potential contributors to disability within these tsetse-infested areas. Incorporating reports on disability from the national census appears to be an important surveillance tool that would enhance future HAT surveillance programs in sub-Saharan Africa.", "publicationTitle": "PLoS Negl Trop Dis", "publisher": "", "place": "", "date": "February 08, 2011", "volume": "5", "issue": "2", "section": "", "partNumber": "", "partTitle": "", "pages": "e957", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "PLoS Negl Trop Dis", "DOI": "10.1371/journal.pntd.0000957", "citationKey": "", "url": "http://dx.doi.org/10.1371/journal.pntd.0000957", "accessDate": "2011-02-23T14:19:50Z", "PMID": "", "PMCID": "", "ISSN": "", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "", "libraryCatalog": "PLoS Negl Trop Dis", "callNumber": "", "rights": "", "extra": "", "tags": [], "collections": [ "TZNGZ92U" ], "relations": {}, "dateAdded": "2011-12-05T09:45:09Z", "dateModified": "2011-12-05T09:45:09Z" } }, { "key": "TJZESJPX", "version": 1, "library": { "type": "group", "id": 62016, "name": "xavier.robin", "links": { "alternate": { "href": "https://www.zotero.org/groups/xavier.robin", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/62016/items/TJZESJPX", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/xavier.robin/items/TJZESJPX", "type": "text/html" } }, "meta": { "createdByUser": { "id": 12953, "username": "Calimo", "name": "Xavier Robin", "links": { "alternate": { "href": "https://www.zotero.org/calimo", "type": "text/html" } } }, "creatorSummary": "Breitwieser et al.", "parsedDate": "2011", "numChildren": 0 }, "data": { "key": "TJZESJPX", "version": 1, "itemType": "journalArticle", "title": "General statistical modeling of data from protein relative expression isobaric tags", "creators": [ { "creatorType": "author", "firstName": "Florian P.", "lastName": "Breitwieser" }, { "creatorType": "author", "firstName": "André", "lastName": "Müller" }, { "creatorType": "author", "firstName": "Loïc", "lastName": "Dayon" }, { "creatorType": "author", "firstName": "Thomas", "lastName": "Köcher" }, { "creatorType": "author", "firstName": "Alexandre", "lastName": "Hainard" }, { "creatorType": "author", "firstName": "Peter", "lastName": "Pichler" }, { "creatorType": "author", "firstName": "Ursula", "lastName": "Schmidt-Erfurth" }, { "creatorType": "author", "firstName": "Giulio", "lastName": "Superti-Furga" }, { "creatorType": "author", "firstName": "Jean-Charles", "lastName": "Sanchez" }, { "creatorType": "author", "firstName": "Karl", "lastName": "Mechtler" }, { "creatorType": "author", "firstName": "Keiryn L.", "lastName": "Bennett" }, { "creatorType": "author", "firstName": "Jacques", "lastName": "Colinge" } ], "abstractNote": "", "publicationTitle": "Journal of Proteome Research", "publisher": "", "place": "", "date": "juin 03, 2011", "volume": "10", "issue": "6", "section": "", "partNumber": "", "partTitle": "", "pages": "2758-2766", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "", "DOI": "10.1021/pr1012784", "citationKey": "", "url": "http://dx.doi.org/10.1021/pr1012784", "accessDate": "2011-05-03T06:47:05Z", "PMID": "", "PMCID": "", "ISSN": "", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "", "libraryCatalog": "ACS Publications", "callNumber": "", "rights": "", "extra": "", "tags": [], "collections": [ "TZNGZ92U" ], "relations": {}, "dateAdded": "2011-12-05T09:45:09Z", "dateModified": "2011-12-05T09:45:09Z" } }, { "key": "AQI4SB67", "version": 1, "library": { "type": "group", "id": 62016, "name": "xavier.robin", "links": { "alternate": { "href": "https://www.zotero.org/groups/xavier.robin", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/62016/items/AQI4SB67", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/xavier.robin/items/AQI4SB67", "type": "text/html" } }, "meta": { "createdByUser": { "id": 12953, "username": "Calimo", "name": "Xavier Robin", "links": { "alternate": { "href": "https://www.zotero.org/calimo", "type": "text/html" } } }, "creatorSummary": "Kennedy", "parsedDate": "2011", "numChildren": 0 }, "data": { "key": "AQI4SB67", "version": 1, "itemType": "journalArticle", "title": "Difficulties in Diagnostic Staging of Human African Trypanosomiasis", "creators": [ { "creatorType": "author", "firstName": "Peter G. E.", "lastName": "Kennedy" } ], "abstractNote": "Human African trypanosomiasis (HAT), also known as sleeping sickness, continues to be a major hazard to human health in 36 countries in sub-Saharan Africa. One of the most important problems in disease management is the considerable difficulty in distinguishing the early (hemolymphatic) from the late (encephalitic) stage when the parasites have crossed the blood-brain barrier to enter the Central Nervous System (CNS). Treatment of the two stages is different with the highly toxic arsenical drug melarsoprol being the most commonly used therapy for CNS disease. Since melarsoprol kills 5% of treated patients, it is vital to develop reliable and agreed diagnostic staging markers for HAT. Although the current WHO staging criteria on the cerebrospinal fluid (CSF) are the most commonly used, there is still a lack of consensus as to their efficacy which has driven the current search for improved methods of HAT diagnostic staging which are considered here.", "publicationTitle": "Journal of Neuroparasitology", "publisher": "", "place": "", "date": "2011", "volume": "2", "issue": "", "section": "", "partNumber": "", "partTitle": "", "pages": "N110601", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "", "DOI": "10.4303/jnp/N110601", "citationKey": "", "url": "http://www.ashdin.com/journals/JNP/N110601.aspx", "accessDate": "", "PMID": "", "PMCID": "", "ISSN": "", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "", "libraryCatalog": "", "callNumber": "", "rights": "", "extra": "", "tags": [], "collections": [ "4G5P8GBH", "E6BR434W", "TZNGZ92U" ], "relations": {}, "dateAdded": "2011-12-05T09:44:51Z", "dateModified": "2011-12-05T09:44:51Z" } }, { "key": "GUBBNT6K", "version": 1, "library": { "type": "group", "id": 62016, "name": "xavier.robin", "links": { "alternate": { "href": "https://www.zotero.org/groups/xavier.robin", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/62016/items/GUBBNT6K", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/xavier.robin/items/GUBBNT6K", "type": "text/html" } }, "meta": { "createdByUser": { "id": 12953, "username": "Calimo", "name": "Xavier Robin", "links": { "alternate": { "href": "https://www.zotero.org/calimo", "type": "text/html" } } }, "creatorSummary": "Geiger et al.", "parsedDate": "2011", "numChildren": 0 }, "data": { "key": "GUBBNT6K", "version": 1, "itemType": "journalArticle", "title": "Transcriptomics and proteomics in human African trypanosomiasis: Current status and perspectives", "creators": [ { "creatorType": "author", "firstName": "Anne", "lastName": "Geiger" }, { "creatorType": "author", "firstName": "Gustave", "lastName": "Simo" }, { "creatorType": "author", "firstName": "Pascal", "lastName": "Grébaut" }, { "creatorType": "author", "firstName": "Jean-Benoît", "lastName": "Peltier" }, { "creatorType": "author", "firstName": "Gérard", "lastName": "Cuny" }, { "creatorType": "author", "firstName": "Philippe", "lastName": "Holzmuller" } ], "abstractNote": "Human African trypanosomiasis, or sleeping sickness, is a neglected vector-borne parasitic disease caused by protozoa of the species Trypanosoma brucei sensu lato. Within this complex species, T. b. gambiense is responsible for the chronic form of sleeping sickness in Western and Central Africa, whereas T. b. rhodesiense causes the acute form of the disease in East Africa. Presently, 1.5 million disability-adjusted life years (DALYs) per year are lost due to sleeping sickness. In addition, on the basis of the mortality, the disease is ranked ninth out of 25 human infectious and parasitic diseases in Africa. Diagnosis is complex and needs the intervention of a specialized skilled staff; treatment is difficult and expensive and has potentially life-threatening side effects. The use of transcriptomic and proteomic technologies, currently in rapid development and increasing in sensitivity and discriminating power, is already generating a large panel of promising results. The objective of these technologies is to significantly increase our knowledge of the molecular mechanisms governing the parasite establishment in its vector, the development cycle of the parasite during the parasite's intra-vector life, its interactions with the fly and the other microbial inhabitants of the gut, and finally human host-trypanosome interactions. Such fundamental investigations are expected to provide opportunities to identify key molecular events that would constitute accurate targets for further development of tools dedicated to field work for early, sensitive, and stage-discriminant diagnosis, epidemiology, new chemotherapy, and potentially vaccine development, all of which will contribute to fighting the disease. The present review highlights the contributions of the transcriptomic and proteomic analyses developed thus far in order to identify potential targets (genes or proteins) and biological pathways that may constitute a critical step in the identification of new targets for the development of new tools for diagnostic and therapeutic purposes.", "publicationTitle": "Journal of Proteomics", "publisher": "", "place": "", "date": "août 24, 2011", "volume": "74", "issue": "9", "section": "", "partNumber": "", "partTitle": "", "pages": "1625-1643", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "", "DOI": "16/j.jprot.2011.01.016", "citationKey": "", "url": "http://www.sciencedirect.com/science/article/pii/S1874391911000339", "accessDate": "2011-08-31T10:47:51Z", "PMID": "", "PMCID": "", "ISSN": "1874-3919", "archive": "", "archiveLocation": "", "shortTitle": "Transcriptomics and proteomics in human African trypanosomiasis", "language": "", "libraryCatalog": "ScienceDirect", "callNumber": "", "rights": "", "extra": "", "tags": [], "collections": [ "4G5P8GBH", "E6BR434W", "TZNGZ92U" ], "relations": {}, "dateAdded": "2011-12-05T09:44:51Z", "dateModified": "2011-12-05T09:44:51Z" } } ]