[ { "key": "QQZG7W4I", "version": 213, "library": { "type": "group", "id": 62016, "name": "xavier.robin", "links": { "alternate": { "href": "https://www.zotero.org/groups/xavier.robin", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/62016/items/QQZG7W4I", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/xavier.robin/items/QQZG7W4I", "type": "text/html" }, "up": { "href": "https://api.zotero.org/groups/62016/items/BSP9T78B", "type": "application/json" } }, "meta": { "createdByUser": { "id": 12953, "username": "Calimo", "name": "Xavier Robin", "links": { "alternate": { "href": "https://www.zotero.org/calimo", "type": "text/html" } } } }, "data": { "key": "QQZG7W4I", "version": 213, "parentItem": "BSP9T78B", "itemType": "attachment", "linkMode": "linked_url", "title": "PubMed entry", "accessDate": "2014-06-09T08:15:53Z", "url": "http://www.ncbi.nlm.nih.gov/pubmed/24789741", "note": "", "contentType": "text/html", "charset": "", "tags": [], "relations": {}, "dateAdded": "2014-06-09T08:16:10Z", "dateModified": "2014-06-09T08:16:10Z" } }, { "key": "BSP9T78B", "version": 213, "library": { "type": "group", "id": 62016, "name": "xavier.robin", "links": { "alternate": { "href": "https://www.zotero.org/groups/xavier.robin", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/62016/items/BSP9T78B", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/xavier.robin/items/BSP9T78B", "type": "text/html" } }, "meta": { "createdByUser": { "id": 12953, "username": "Calimo", "name": "Xavier Robin", "links": { "alternate": { "href": "https://www.zotero.org/calimo", "type": "text/html" } } }, "creatorSummary": "Sternberg and Mitchell", "parsedDate": "2014-04-30", "numChildren": 1 }, "data": { "key": "BSP9T78B", "version": 213, "itemType": "journalArticle", "title": "Plasma neuronal specific enolase: a potential stage diagnostic marker in human African trypanosomiasis", "creators": [ { "creatorType": "author", "firstName": "Jeremy M.", "lastName": "Sternberg" }, { "creatorType": "author", "firstName": "Julia A.", "lastName": "Mitchell" } ], "abstractNote": "Background This study was carried out to determine the potential of neuronal specific enolase (NSE) as a stage diagnostic marker in human African trypanosomiasis.\nMethods Plasma and cerebrospinal fluid were obtained from a cohort of Trypanosoma brucei rhodesiense infected patients and non-infected controls. Neuronal specific enolase concentrations were measured by ELISA and analysed in relation to diagnosis and disease-stage data.\nResults Plasma NSE concentration was significantly increased in late-stage patients (median 21 ng/ml), compared to the control (median 11 ng/ml), but not in early-stage patients (median 5.3 ng/ml). Cerebrospinal fluid NSE concentration did not vary between stages.\nConclusion Plasma NSE is a potential stage diagnostic in this cohort and merits further investigation.", "publicationTitle": "Transactions of The Royal Society of Tropical Medicine and Hygiene", "publisher": "", "place": "", "date": "2014-04-30", "volume": "", "issue": "", "section": "", "partNumber": "", "partTitle": "", "pages": "tru065", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "Trans R Soc Trop Med Hyg", "DOI": "10.1093/trstmh/tru065", "citationKey": "", "url": "http://trstmh.oxfordjournals.org/content/early/2014/04/29/trstmh.tru065", "accessDate": "2014-06-09T08:15:53Z", "PMID": "", "PMCID": "", "ISSN": "0035-9203, 1878-3503", "archive": "", "archiveLocation": "", "shortTitle": "Plasma neuronal specific enolase", "language": "en", "libraryCatalog": "trstmh.oxfordjournals.org", "callNumber": "", "rights": "", "extra": "PMID: 24789741", "tags": [ { "tag": "African trypanosomiasis", "type": 1 }, { "tag": "Neuronal specific enolase", "type": 1 }, { "tag": "Stage", "type": 1 } ], "collections": [ "E6BR434W" ], "relations": {}, "dateAdded": "2014-06-09T08:16:10Z", "dateModified": "2014-06-09T08:16:10Z" } }, { "key": "N64ADQC5", "version": 217, "library": { "type": "group", "id": 62016, "name": "xavier.robin", "links": { "alternate": { "href": "https://www.zotero.org/groups/xavier.robin", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/62016/items/N64ADQC5", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/xavier.robin/items/N64ADQC5", "type": "text/html" } }, "meta": { "createdByUser": { "id": 12953, "username": "Calimo", "name": "Xavier Robin", "links": { "alternate": { "href": "https://www.zotero.org/calimo", "type": "text/html" } } }, "creatorSummary": "McKimmie and Michlmayr", "parsedDate": "2014", "numChildren": 0 }, "data": { "key": "N64ADQC5", "version": 217, "itemType": "journalArticle", "title": "Role of CXCL10 in central nervous system inflammation", "creators": [ { "creatorType": "author", "firstName": "Clive", "lastName": "McKimmie" }, { "creatorType": "author", "firstName": "Daniela", "lastName": "Michlmayr" } ], "abstractNote": "", "publicationTitle": "International Journal of Interferon, Cytokine and Mediator Research", "publisher": "", "place": "", "date": "02/2014", "volume": "", "issue": "", "section": "", "partNumber": "", "partTitle": "", "pages": "1", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "", "DOI": "10.2147/IJICMR.S35953", "citationKey": "", "url": "http://www.dovepress.com/role-of-cxcl10-in-central-nervous-system-inflammation-peer-reviewed-article-IJICMR", "accessDate": "2014-04-18T14:02:04Z", "PMID": "", "PMCID": "", "ISSN": "1179-139X", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "en", "libraryCatalog": "CrossRef", "callNumber": "", "rights": "", "extra": "", "tags": [], "collections": [ "8QFQVCUA", "E6BR434W" ], "relations": {}, "dateAdded": "2014-04-18T14:12:13Z", "dateModified": "2014-04-18T14:12:13Z" } }, { "key": "DBMUI368", "version": 191, "library": { "type": "group", "id": 62016, "name": "xavier.robin", "links": { "alternate": { "href": "https://www.zotero.org/groups/xavier.robin", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/62016/items/DBMUI368", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/xavier.robin/items/DBMUI368", "type": "text/html" } }, "meta": { "createdByUser": { "id": 12953, "username": "Calimo", "name": "Xavier Robin", "links": { "alternate": { "href": "https://www.zotero.org/calimo", "type": "text/html" } } }, "creatorSummary": "Nazário", "parsedDate": "2012", "numChildren": 0 }, "data": { "key": "DBMUI368", "version": 191, "itemType": "thesis", "title": "Desenvolvimento de métodos moleculares para a deteção de Trypanosoma spp em glossinas (Diptera: Glossinidae) da República da Guiné Bissau", "creators": [ { "creatorType": "author", "firstName": "Carlos", "lastName": "Nazário" } ], "abstractNote": "As moscas do género Glossina são vetores de patologias provocadas por várias espécies de parasita do género Trypanosoma, como a Tripanossomose Humana Africana e as Tripanossomoses Animais. A correta identificação destes tripanossomas é um ponto fulcral quando se procura determinar o risco de doença que determinadas populações de glossinas podem provocar.\nNo presente trabalho foram utilizadas e adaptadas diversas metodologias de diagnóstico molecular, nomeadamente o PCR em tempo real e o PCR-RFLP, que permitiram, através da utilização de primers genéricos, a determinação da carga parasitária e a correta identificação dos tripanossomas presentes no vetor. Os primers denominados Tryp18SF e Tryp18SR foram desenvolvidos especificamente para serem utilizados na identificação das espécies de tripanossoma utilizando PCR em tempo real com metodologia SYBR® Green I e PCR-RFLP. Os primers denominados Tryp18S2F, Tryp18S2R e sonda Tryp18S2P foram desenvolvidos para a quantificação dos parasitas presentes nas amostras utilizando PCR em tempo real com metodologia Taqman®.\nFoi estudada uma amostra de 762 glossinas provenientes do território da República da Guiné-Bissau, zona atualmente considerada como estando livre de Tripanossomose Humana Africana, mas onde não são realizadas atividades de recenseamento de casos desde o final da ocupação portuguesa.\nDas 762 glossinas utilizadas, 241 estavam infetadas com tripanossomas, o que representa uma percentagem de infeção geral de 31,6 %. Destas glossinas, 28.63 % encontravam-se infetadas com Trypanosoma grayi, 14.11 % com Trypanosoma congolense, 7.05 % com Trypanosoma vivax e 0.83 % com Trypanosoma brucei brucei, tendo as infeções mistas representado 1.66 %. Não foi possível a identificação conclusiva ao nível da espécie em 48.13 % das amostras positivas, tendo estas ficado consideradas como Trypanosoma spp. Não foram identificados vetores infetados com tripanossomas responsáveis pelas patologias humanas.\nA utilização de primers genéricos para a identificação permitiu obviar o número de reações necessárias para identificar corretamente o parasita responsável pela infeção, e este trabalho é de resto a primeira descrição da utilização de primers genéricos que permitam a identificação de Trypanosoma grayi.\nEsta é também a primeira descrição da utilização de PCR em tempo real com metodologia Taqman® para quantificação de tripanossomas, e a sua utilização com primers genéricos aumenta a aplicabilidade em estudos epidemiológicos de grande escala.\n\n\nPalavras-chave: PCR em tempo real; PCR-RFLP; República da Guiné-Bissau; Glossina; Trypanosoma; Tripanossomose.", "thesisType": "", "university": "", "place": "", "date": "2012", "series": "", "seriesNumber": "", "numPages": "", "DOI": "", "ISBN": "", "citationKey": "", "url": "http://run.unl.pt/handle/10362/11131", "accessDate": "2014-04-18T14:09:06Z", "ISSN": "", "archive": "", "archiveLocation": "", "shortTitle": "Desenvolvimento de métodos moleculares para a deteção de Trypanosoma spp em glossinas (Diptera", "language": "other", "libraryCatalog": "run.unl.pt", "callNumber": "", "rights": "", "extra": "", "tags": [ { "tag": "masterThesis", "type": 1 } ], "collections": [ "E6BR434W" ], "relations": {}, "dateAdded": "2014-04-18T14:12:13Z", "dateModified": "2014-04-18T14:12:13Z" } }, { "key": "3USD69HR", "version": 191, "library": { "type": "group", "id": 62016, "name": "xavier.robin", "links": { "alternate": { "href": "https://www.zotero.org/groups/xavier.robin", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/62016/items/3USD69HR", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/xavier.robin/items/3USD69HR", "type": "text/html" } }, "meta": { "createdByUser": { "id": 12953, "username": "Calimo", "name": "Xavier Robin", "links": { "alternate": { "href": "https://www.zotero.org/calimo", "type": "text/html" } } }, "creatorSummary": "Tiberti et al.", "parsedDate": "2013", "numChildren": 0 }, "data": { "key": "3USD69HR", "version": 191, "itemType": "journalArticle", "title": "Translation of human African trypanosomiasis biomarkers towards field application", "creators": [ { "creatorType": "author", "firstName": "Natalia", "lastName": "Tiberti" }, { "creatorType": "author", "firstName": "Alexandre", "lastName": "Hainard" }, { "creatorType": "author", "firstName": "Jean-Charles", "lastName": "Sanchez" } ], "abstractNote": "", "publicationTitle": "Translational Proteomics", "publisher": "", "place": "", "date": "2013", "volume": "1", "issue": "1", "section": "", "partNumber": "", "partTitle": "", "pages": "12–24", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "", "DOI": "", "citationKey": "", "url": "http://www.sciencedirect.com/science/article/pii/S2212963413000041", "accessDate": "2014-04-18T14:05:39Z", "PMID": "", "PMCID": "", "ISSN": "", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "", "libraryCatalog": "Google Scholar", "callNumber": "", "rights": "", "extra": "", "tags": [], "collections": [ "E6BR434W" ], "relations": {}, "dateAdded": "2014-04-18T14:12:13Z", "dateModified": "2014-04-18T14:12:13Z" } }, { "key": "4KC5JB95", "version": 191, "library": { "type": "group", "id": 62016, "name": "xavier.robin", "links": { "alternate": { "href": "https://www.zotero.org/groups/xavier.robin", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/62016/items/4KC5JB95", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/xavier.robin/items/4KC5JB95", "type": "text/html" } }, "meta": { "createdByUser": { "id": 12953, "username": "Calimo", "name": "Xavier Robin", "links": { "alternate": { "href": "https://www.zotero.org/calimo", "type": "text/html" } } }, "creatorSummary": "Khosravi", "numChildren": 0 }, "data": { "key": "4KC5JB95", "version": 191, "itemType": "thesis", "title": "Potential biomarker expression and variation during the course of infection in experimentally infected Vervet Monkeys by T. b. rhodesiense", "creators": [ { "creatorType": "author", "firstName": "Sanaz Zarghami", "lastName": "Khosravi" } ], "abstractNote": "", "thesisType": "", "university": "", "place": "", "date": "", "series": "", "seriesNumber": "", "numPages": "", "DOI": "", "ISBN": "", "citationKey": "", "url": "http://www.mpb.unige.ch/reports/rap_SanazZarghami.pdf", "accessDate": "2014-04-18T14:05:39Z", "ISSN": "", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "", "libraryCatalog": "Google Scholar", "callNumber": "", "rights": "", "extra": "", "tags": [], "collections": [ "E6BR434W" ], "relations": {}, "dateAdded": "2014-04-18T14:12:13Z", "dateModified": "2014-04-18T14:12:13Z" } }, { "key": "5Z9U6KEW", "version": 191, "library": { "type": "group", "id": 62016, "name": "xavier.robin", "links": { "alternate": { "href": "https://www.zotero.org/groups/xavier.robin", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/62016/items/5Z9U6KEW", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/xavier.robin/items/5Z9U6KEW", "type": "text/html" } }, "meta": { "createdByUser": { "id": 12953, "username": "Calimo", "name": "Xavier Robin", "links": { "alternate": { "href": "https://www.zotero.org/calimo", "type": "text/html" } } }, "creatorSummary": "Allard et al.", "parsedDate": "2011", "numChildren": 0 }, "data": { "key": "5Z9U6KEW", "version": 191, "itemType": "book", "title": "Diagnostic method for brain damage-related disorders", "creators": [ { "creatorType": "author", "firstName": "Laure", "lastName": "Allard" }, { "creatorType": "author", "firstName": "Denis F.", "lastName": "Hochstrasser" }, { "creatorType": "author", "firstName": "Pierre", "lastName": "Lescuyer" }, { "creatorType": "author", "firstName": "Jean-Charles", "lastName": "Sanchez" } ], "abstractNote": "", "series": "", "seriesNumber": "", "volume": "", "numberOfVolumes": "", "edition": "", "date": "juin 2011", "publisher": "Google Patents", "place": "", "originalDate": "", "originalPublisher": "", "originalPlace": "", "format": "", "numPages": "", "ISBN": "", "DOI": "", "citationKey": "", "url": "http://www.google.com/patents/US7955804", "accessDate": "2014-04-18T14:05:39Z", "ISSN": "", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "", "libraryCatalog": "Google Scholar", "callNumber": "", "rights": "", "extra": "US Patent 7,955,804", "tags": [], "collections": [ "E6BR434W" ], "relations": {}, "dateAdded": "2014-04-18T14:12:13Z", "dateModified": "2014-04-18T14:12:13Z" } }, { "key": "WRIBRBF6", "version": 191, "library": { "type": "group", "id": 62016, "name": "xavier.robin", "links": { "alternate": { "href": "https://www.zotero.org/groups/xavier.robin", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/62016/items/WRIBRBF6", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/xavier.robin/items/WRIBRBF6", "type": "text/html" } }, "meta": { "createdByUser": { "id": 12953, "username": "Calimo", "name": "Xavier Robin", "links": { "alternate": { "href": "https://www.zotero.org/calimo", "type": "text/html" } } }, "creatorSummary": "Giordani", "parsedDate": "2011", "numChildren": 0 }, "data": { "key": "WRIBRBF6", "version": 191, "itemType": "thesis", "title": "New approaches to fluorescence-based diagnostics for human African trypanosomiasis", "creators": [ { "creatorType": "author", "firstName": "Federica", "lastName": "Giordani" } ], "abstractNote": "", "thesisType": "", "university": "University of Glasgow", "place": "", "date": "2011", "series": "", "seriesNumber": "", "numPages": "", "DOI": "", "ISBN": "", "citationKey": "", "url": "http://theses.gla.ac.uk/2454/", "accessDate": "2014-04-18T14:05:39Z", "ISSN": "", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "", "libraryCatalog": "Google Scholar", "callNumber": "", "rights": "", "extra": "", "tags": [], "collections": [ "E6BR434W" ], "relations": {}, "dateAdded": "2014-04-18T14:12:13Z", "dateModified": "2014-04-18T14:12:13Z" } }, { "key": "JHDD2A78", "version": 169, "library": { "type": "group", "id": 62016, "name": "xavier.robin", "links": { "alternate": { "href": "https://www.zotero.org/groups/xavier.robin", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/62016/items/JHDD2A78", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/xavier.robin/items/JHDD2A78", "type": "text/html" } }, "meta": { "createdByUser": { "id": 12953, "username": "Calimo", "name": "Xavier Robin", "links": { "alternate": { "href": "https://www.zotero.org/calimo", "type": "text/html" } } }, "creatorSummary": "Dayon et al.", "parsedDate": "2013-09-26", "numChildren": 0 }, "data": { "key": "JHDD2A78", "version": 169, "itemType": "patent", "title": "Diagnostic Methods", "creators": [ { "creatorType": "inventor", "firstName": "Loic Gerard", "lastName": "Dayon" }, { "creatorType": "inventor", "firstName": "Jean-charles", "lastName": "Sanchez" }, { "creatorType": "inventor", "firstName": "Joan Montaner", "lastName": "Villalonga" } ], "abstractNote": "The invention relates to a method of aiding the diagnosis of acute brain damage in a subject, said method comprising (i) assaying the concentration of at least one oxidative stress polypeptide selected from the group consisting of: PRDX1, PRDX6 and GSTP1 in a sample from said subject; and (ii) assaying the concentration of at least one further polypeptide selected from Panel A; (Hi) comparing the concentrations of (i) and (ii) to the concentrations of the polypeptides in a reference standard and determining quantitative ratios for said polypeptides; (iv) wherein a finding of a quantitative ratio of each of the assayed polypeptides in the sample to the polypeptides in the reference standard of greater than 1.3 indicates an increased likelihood of acute brain damage having occurred in said subject.", "place": "", "country": "United States", "assignee": "UNIVERSITE DE GENEVE (Geneva 4, CH) ELECTROPHORETICS LIMITED (Cobham Surrey, GB)", "issuingAuthority": "", "patentNumber": "20130252834", "filingDate": "2011-05-23 05/23/2011", "pages": "", "applicationNumber": "13/699444", "priorityNumbers": "", "issueDate": "09/26/2013", "priorityDate": "", "references": "", "legalStatus": "", "DOI": "", "citationKey": "", "url": "http://www.freepatentsonline.com/y2013/0252834.html", "accessDate": "2014-02-28T18:34:07Z", "shortTitle": "", "language": "", "rights": "", "extra": "", "tags": [], "collections": [ "E6BR434W" ], "relations": {}, "dateAdded": "2014-02-28T18:57:03Z", "dateModified": "2014-02-28T18:57:03Z" } }, { "key": "6FZPBZD7", "version": 169, "library": { "type": "group", "id": 62016, "name": "xavier.robin", "links": { "alternate": { "href": "https://www.zotero.org/groups/xavier.robin", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/62016/items/6FZPBZD7", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/xavier.robin/items/6FZPBZD7", "type": "text/html" } }, "meta": { "createdByUser": { "id": 12953, "username": "Calimo", "name": "Xavier Robin", "links": { "alternate": { "href": "https://www.zotero.org/calimo", "type": "text/html" } } }, "creatorSummary": "Büscher", "parsedDate": "2014-01-01", "numChildren": 0 }, "data": { "key": "6FZPBZD7", "version": 169, "itemType": "bookSection", "title": "Diagnosis of African Trypanosomiasis", "creators": [ { "creatorType": "author", "firstName": "Philippe", "lastName": "Büscher" }, { "creatorType": "editor", "firstName": "Stefan", "lastName": "Magez" }, { "creatorType": "editor", "firstName": "Magdalena", "lastName": "Radwanska" } ], "abstractNote": "In African trypanosomiasis, diagnosis is complex since several diseases are caused by different parasites and occur in different host species giving rise to a variety of parasite–host relationships. At one end of the spectrum we find acute or chronic but fatal diseases, while at the other end long-lasting subclinical and almost commensal infections are observed. The result is that all diagnostic methods, whether clinical or molecular, have their limitations that will define how, where and for what particular trypanosomiasis they will eventually be applied, alone or in combination. Research on diagnosis of African trypanosomiasis perfectly reflects the technological and socio-economical environment wherein it is conducted. After the discovery of African trypanosomes causing sleeping sickness and nagana in the early twentieth century, refinement of clinical diagnosis was soon followed by the development of improved parasitological methods. Later, serological and molecular diagnostics appeared but hardly found their way to the non-academic end user with one exception, a direct agglutination test for gambiense sleeping sickness. Only in the last decade, African trypanosomiases were freed from their status of neglected tropical diseases and received much more attention from public and private financial donors and from researchers. This evolution has led to major breakthroughs in diagnostics development that may have a huge impact on control of human and animal African trypanosomiases.", "bookTitle": "Trypanosomes and Trypanosomiasis", "series": "", "seriesNumber": "", "volume": "", "numberOfVolumes": "", "edition": "", "date": "2014/01/01", "publisher": "Springer Vienna", "place": "", "originalDate": "", "originalPublisher": "", "originalPlace": "", "format": "", "pages": "189-216", "ISBN": "978-3-7091-1555-8, 978-3-7091-1556-5", "DOI": "", "citationKey": "", "url": "http://link.springer.com/chapter/10.1007/978-3-7091-1556-5_8", "accessDate": "2014-02-28T18:38:36Z", "ISSN": "", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "en", "libraryCatalog": "link.springer.com", "callNumber": "", "rights": "©2014 Springer-Verlag Wien", "extra": "", "tags": [ { "tag": "Infectious Diseases", "type": 1 }, { "tag": "Medical Microbiology", "type": 1 }, { "tag": "Parasitology", "type": 1 } ], "collections": [ "E6BR434W", "XRTS6Q6R" ], "relations": {}, "dateAdded": "2014-02-28T18:50:57Z", "dateModified": "2014-02-28T18:50:57Z" } }, { "key": "RSS5GSDH", "version": 169, "library": { "type": "group", "id": 62016, "name": "xavier.robin", "links": { "alternate": { "href": "https://www.zotero.org/groups/xavier.robin", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/62016/items/RSS5GSDH", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/xavier.robin/items/RSS5GSDH", "type": "text/html" } }, "meta": { "createdByUser": { "id": 12953, "username": "Calimo", "name": "Xavier Robin", "links": { "alternate": { "href": "https://www.zotero.org/calimo", "type": "text/html" } } }, "creatorSummary": "Masocha", "parsedDate": "2013", "numChildren": 0 }, "data": { "key": "RSS5GSDH", "version": 169, "itemType": "journalArticle", "title": "Role of chemokines and cytokines in the neuropathogenesis of African trypanosomiasis", "creators": [ { "creatorType": "author", "firstName": "Willias", "lastName": "Masocha" } ], "abstractNote": "", "publicationTitle": "World", "publisher": "", "place": "", "date": "2013", "volume": "3", "issue": "4", "section": "", "partNumber": "", "partTitle": "", "pages": "79–85", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "", "DOI": "", "citationKey": "", "url": "http://www.wjgnet.com/2220-3176/full/v3/i4/79.htm", "accessDate": "2014-02-28T18:26:08Z", "PMID": "", "PMCID": "", "ISSN": "", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "", "libraryCatalog": "Google Scholar", "callNumber": "", "rights": "", "extra": "", "tags": [], "collections": [ "4G5P8GBH", "8QFQVCUA", "E6BR434W", "XRTS6Q6R" ], "relations": {}, "dateAdded": "2014-02-28T18:46:21Z", "dateModified": "2014-02-28T18:46:21Z" } }, { "key": "DZ27NEZ3", "version": 173, "library": { "type": "group", "id": 62016, "name": "xavier.robin", "links": { "alternate": { "href": "https://www.zotero.org/groups/xavier.robin", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/62016/items/DZ27NEZ3", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/xavier.robin/items/DZ27NEZ3", "type": "text/html" } }, "meta": { "createdByUser": { "id": 12953, "username": "Calimo", "name": "Xavier Robin", "links": { "alternate": { "href": "https://www.zotero.org/calimo", "type": "text/html" } } }, "creatorSummary": "Nyawira Maranga et al.", "parsedDate": "2013-09-30", "numChildren": 0 }, "data": { "key": "DZ27NEZ3", "version": 173, "itemType": "journalArticle", "title": "IL-6 is Upregulated in Late-Stage Disease in Monkeys Experimentally Infected with Trypanosoma brucei rhodesiense", "creators": [ { "creatorType": "author", "firstName": "Dawn", "lastName": "Nyawira Maranga" }, { "creatorType": "author", "firstName": "John Maina", "lastName": "Kagira" }, { "creatorType": "author", "firstName": "Christopher Kariuki", "lastName": "Kinyanjui" }, { "creatorType": "author", "firstName": "Simon", "lastName": "Muturi Karanja" }, { "creatorType": "author", "firstName": "Naomi", "lastName": "Wangari Maina" }, { "creatorType": "author", "firstName": "Maina", "lastName": "Ngotho" } ], "abstractNote": "The management of human African trypanosomiasis (HAT) is constrained by lack of simple-to-use diagnostic, staging, and treatment tools. The search for novel biomarkers is, therefore, essential in the fight against HAT. The current study aimed at investigating the potential of IL-6 as an adjunct parameter for HAT stage determination in vervet monkey model. Four adult vervet monkeys (Chlorocebus aethiops) were experimentally infected with Trypanosoma brucei rhodesiense and treated subcuratively at 28 days after infection (dpi) to induce late stage disease. Three noninfected monkeys formed the control group. Cerebrospinal fluid (CSF) and blood samples were obtained at weekly intervals and assessed for various biological parameters. A typical HAT-like infection was observed. The late stage was characterized by significant () elevation of CSF IL-6, white blood cell count, and total protein starting 35 dpi with peak levels of these parameters coinciding with relapse parasitaemia. Brain immunohistochemical staining revealed an increase in brain glial fibrillary acidic protein expression indicative of reactive astrogliosis in infected animals which were euthanized in late-stage disease. The elevation of IL-6 in CSF which accompanied other HAT biomarkers indicates onset of parasite neuroinvasion and show potential for use as an adjunct late-stage disease biomarker in the Rhodesian sleeping sickness.", "publicationTitle": "Clinical and Developmental Immunology", "publisher": "", "place": "", "date": "2013/09/30", "volume": "2013", "issue": "", "section": "", "partNumber": "", "partTitle": "", "pages": "", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "", "DOI": "10.1155/2013/320509", "citationKey": "", "url": "http://www.hindawi.com/journals/cdi/2013/320509/abs/", "accessDate": "2013-11-24T11:02:26Z", "PMID": "", "PMCID": "", "ISSN": "1740-2522", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "en", "libraryCatalog": "www.hindawi.com", "callNumber": "", "rights": "", "extra": "", "tags": [], "collections": [ "8QFQVCUA", "E6BR434W", "TZNGZ92U", "XRTS6Q6R" ], "relations": {}, "dateAdded": "2013-11-24T11:04:09Z", "dateModified": "2013-11-24T11:04:43Z" } }, { "key": "XIHVAMTN", "version": 173, "library": { "type": "group", "id": 62016, "name": "xavier.robin", "links": { "alternate": { "href": "https://www.zotero.org/groups/xavier.robin", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/62016/items/XIHVAMTN", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/xavier.robin/items/XIHVAMTN", "type": "text/html" } }, "meta": { "createdByUser": { "id": 12953, "username": "Calimo", "name": "Xavier Robin", "links": { "alternate": { "href": "https://www.zotero.org/calimo", "type": "text/html" } } }, "creatorSummary": "Holzmuller et al.", "parsedDate": "2013", "numChildren": 0 }, "data": { "key": "XIHVAMTN", "version": 173, "itemType": "journalArticle", "title": "Proteomics: a new way to improve human African trypanosomiasis diagnosis?", "creators": [ { "creatorType": "author", "firstName": "Philippe", "lastName": "Holzmuller" }, { "creatorType": "author", "firstName": "Pascal", "lastName": "Grébaut" }, { "creatorType": "author", "firstName": "Silla", "lastName": "Semballa" }, { "creatorType": "author", "firstName": "Mary Isabel", "lastName": "Gonzatti" }, { "creatorType": "author", "firstName": "Anne", "lastName": "Geiger" } ], "abstractNote": "", "publicationTitle": "Expert Review of Proteomics", "publisher": "", "place": "", "date": "06/2013", "volume": "10", "issue": "3", "section": "", "partNumber": "", "partTitle": "", "pages": "289-301", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "", "DOI": "10.1586/epr.13.14", "citationKey": "", "url": "http://www.expert-reviews.com/doi/abs/10.1586/epr.13.14", "accessDate": "2013-11-24T11:02:56Z", "PMID": "", "PMCID": "", "ISSN": "1478-9450, 1744-8387", "archive": "", "archiveLocation": "", "shortTitle": "Proteomics", "language": "", "libraryCatalog": "CrossRef", "callNumber": "", "rights": "", "extra": "", "tags": [], "collections": [ "4G5P8GBH", "8QFQVCUA", "E6BR434W", "TZNGZ92U", "XRTS6Q6R" ], "relations": {}, "dateAdded": "2013-11-24T11:04:09Z", "dateModified": "2013-11-24T11:04:09Z" } }, { "key": "HNBGQQ5D", "version": 173, "library": { "type": "group", "id": 62016, "name": "xavier.robin", "links": { "alternate": { "href": "https://www.zotero.org/groups/xavier.robin", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/62016/items/HNBGQQ5D", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/xavier.robin/items/HNBGQQ5D", "type": "text/html" } }, "meta": { "createdByUser": { "id": 12953, "username": "Calimo", "name": "Xavier Robin", "links": { "alternate": { "href": "https://www.zotero.org/calimo", "type": "text/html" } } }, "creatorSummary": "Holzmuller et al.", "parsedDate": "2014-01-01", "numChildren": 0 }, "data": { "key": "HNBGQQ5D", "version": 173, "itemType": "bookSection", "title": "Proteomics and African Trypanosomes: Shedding New Light on Host–Vector–Parasite Interactions and Impact on Control Methods", "creators": [ { "creatorType": "author", "firstName": "Philippe", "lastName": "Holzmuller" }, { "creatorType": "author", "firstName": "Pascal", "lastName": "Grébaut" }, { "creatorType": "author", "firstName": "Anne", "lastName": "Geiger" }, { "creatorType": "editor", "firstName": "Stefan", "lastName": "Magez" }, { "creatorType": "editor", "firstName": "Magdalena", "lastName": "Radwanska" } ], "abstractNote": "Most mammalian and vector host species have acquired strategies by selective pressure to mislead the trypanosome and to win the fight during their molecular dialogue. Due to the same evolutionary pressure, trypanosomes have acquired strategies to bypass the host defences and to ensure the completion of their complex life cycles. Elucidation of these complex molecular crosstalks will improve the understanding of trypanosomes’ variability with respect to virulence and pathogenicity, will help to define trypansome-specific host biomarkers and will help to refine control strategies for African trypanosomoses. Advances in proteomics applications have provided new insights on African trypanosomes and on the biochemical interactions with their tsetse vectors and mammalian hosts. In this chapter, we present the interest of proteomics to characterise trypanosomes–hosts interactions, a synthetic review of proteomics studies performed on the parasite and its respective hosts, a discussion on the contributions and pitfalls of using diverse proteomics tools, a view for future prospects on proteomics dedicated to African trypanosomes and a projection of new conceptual approaches (i.e. metabolomics, interactomics, population proteomics) to accurately decipher insect vector–trypanosome–mammalian host interactions, with the idea of further developing new tools to improve trypanosomoses control.", "bookTitle": "Trypanosomes and Trypanosomiasis", "series": "", "seriesNumber": "", "volume": "", "numberOfVolumes": "", "edition": "", "date": "2014/01/01", "publisher": "Springer Vienna", "place": "", "originalDate": "", "originalPublisher": "", "originalPlace": "", "format": "", "pages": "161-187", "ISBN": "978-3-7091-1555-8, 978-3-7091-1556-5", "DOI": "", "citationKey": "", "url": "http://link.springer.com/chapter/10.1007/978-3-7091-1556-5_7", "accessDate": "2013-11-24T11:02:43Z", "ISSN": "", "archive": "", "archiveLocation": "", "shortTitle": "Proteomics and African Trypanosomes", "language": "en", "libraryCatalog": "link.springer.com", "callNumber": "", "rights": "©2014 Springer-Verlag Wien", "extra": "", "tags": [ { "tag": "Infectious Diseases", "type": 1 }, { "tag": "Medical Microbiology", "type": 1 }, { "tag": "Parasitology", "type": 1 } ], "collections": [ "4G5P8GBH", "8QFQVCUA", "E6BR434W", "K2HIHR6Q", "TZNGZ92U", "XRTS6Q6R" ], "relations": {}, "dateAdded": "2013-11-24T11:04:09Z", "dateModified": "2013-11-24T11:04:09Z" } }, { "key": "VRIKQ5PB", "version": 109, "library": { "type": "group", "id": 62016, "name": "xavier.robin", "links": { "alternate": { "href": "https://www.zotero.org/groups/xavier.robin", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/62016/items/VRIKQ5PB", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/xavier.robin/items/VRIKQ5PB", "type": "text/html" } }, "meta": { "createdByUser": { "id": 12953, "username": "Calimo", "name": "Xavier Robin", "links": { "alternate": { "href": "https://www.zotero.org/calimo", "type": "text/html" } } }, "creatorSummary": "Holzmuller et al.", "parsedDate": "2013", "numChildren": 0 }, "data": { "key": "VRIKQ5PB", "version": 109, "itemType": "journalArticle", "title": "Proteomics: a new way to improve human African trypanosomiasis diagnosis?", "creators": [ { "creatorType": "author", "firstName": "Philippe", "lastName": "Holzmuller" }, { "creatorType": "author", "firstName": "Pascal", "lastName": "Grébaut" }, { "creatorType": "author", "firstName": "Silla", "lastName": "Semballa" }, { "creatorType": "author", "firstName": "Mary Isabel", "lastName": "Gonzatti" }, { "creatorType": "author", "firstName": "Anne", "lastName": "Geiger" } ], "abstractNote": "African trypanosomiases, including the human disease referred to as ‘sleeping sickness’ and the animal diseases such as nagana, surra and dourine, are neglected vector-borne diseases that after years of research still need improved diagnosis and chemotherapy. Advances in proteomics offer new tools to define biomarkers, whose expression may reflect host–parasite interactions occurring during the infection. In this review, the authors first describe the current diagnostic tools used to detect a trypanosome infection during field surveys, and then discuss their interests, limits and further evolutions. The authors also report on the contribution of molecular diagnostics, and the recent advances and developments that make it suitable for fieldwork. The authors then explore the recent uses of proteomics technology to define host and parasite biomarkers that allow detection of the infection, the power and constraints of the technology. The authors conclude by discussing the urgent need to use the biomarkers dis...", "publicationTitle": "Expert Review of Proteomics", "publisher": "", "place": "", "date": "2013", "volume": "10", "issue": "3", "section": "", "partNumber": "", "partTitle": "", "pages": "289-301", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "", "DOI": "10.1586/epr.13.14", "citationKey": "", "url": "http://www.expert-reviews.com/doi/abs/10.1586/epr.13.14", "accessDate": "2013-09-03T20:51:35Z", "PMID": "", "PMCID": "", "ISSN": "", "archive": "", "archiveLocation": "", "shortTitle": "Proteomics", "language": "EN", "libraryCatalog": "", "callNumber": "", "rights": "© 2013 Expert Reviews Ltd", "extra": "", "tags": [ { "tag": "Human African trypanosomiasis", "type": 1 }, { "tag": "Proteomics", "type": 1 }, { "tag": "animal trypanosomosis", "type": 1 }, { "tag": "molecular diagnosis", "type": 1 }, { "tag": "serodiagnosis", "type": 1 } ], "collections": [ "E6BR434W" ], "relations": {}, "dateAdded": "2013-09-03T20:52:54Z", "dateModified": "2013-09-03T20:52:54Z" } }, { "key": "99SGHPN9", "version": 69, "library": { "type": "group", "id": 62016, "name": "xavier.robin", "links": { "alternate": { "href": "https://www.zotero.org/groups/xavier.robin", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/62016/items/99SGHPN9", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/xavier.robin/items/99SGHPN9", "type": "text/html" } }, "meta": { "createdByUser": { "id": 12953, "username": "Calimo", "name": "Xavier Robin", "links": { "alternate": { "href": "https://www.zotero.org/calimo", "type": "text/html" } } }, "creatorSummary": "Robin et al.", "parsedDate": "2013", "numChildren": 0 }, "data": { "key": "99SGHPN9", "version": 69, "itemType": "journalArticle", "title": "PanelomiX: A threshold-based algorithm to create panels of biomarkers", "creators": [ { "creatorType": "author", "firstName": "Xavier", "lastName": "Robin" }, { "creatorType": "author", "firstName": "Natacha", "lastName": "Turck" }, { "creatorType": "author", "firstName": "Alexandre", "lastName": "Hainard" }, { "creatorType": "author", "firstName": "Natalia", "lastName": "Tiberti" }, { "creatorType": "author", "firstName": "Frédérique", "lastName": "Lisacek" }, { "creatorType": "author", "firstName": "Jean-Charles", "lastName": "Sanchez" }, { "creatorType": "author", "firstName": "Markus", "lastName": "Müller" } ], "abstractNote": "Abstract \nIn order to increase their predictive power, medical biomarkers can be combined into panels. However, the lack of ready-to-use tools generating interpretable results and implementing rigorous validation standards hampers the more widespread application of panels and their translation into clinical practice. \n \nThe computational toolbox we present here – PanelomiX – uses the iterative combination of biomarkers and thresholds (ICBT) method. This method combines biomarkers and clinical scores by selecting thresholds that provide optimal classification performance. To speed up the calculation for a large number of biomarkers, PanelomiX selects a subset of thresholds and parameters based on the random forest method. The panels’ robustness and performance are analysed by cross-validation (CV) and receiver operating characteristic (ROC) analysis. \n \nUsing 8 biomarkers, we compared this method against classic combination procedures in the determination of outcome for 113 patients with an aneurysmal subarachnoid haemorrhage. The panel classified the patients better than the best single biomarker (p < 0.005) and compared favourably with other off-the-shelf classification methods. \n \nIn conclusion, the PanelomiX toolbox combines biomarkers and evaluates the performance of panels to classify patients better than single markers or other classifiers. The ICBT algorithm proved to be an efficient classifier, the results of which can easily be interpreted.", "publicationTitle": "Translational Proteomics", "publisher": "", "place": "", "date": "2013", "volume": "1", "issue": "1", "section": "", "partNumber": "", "partTitle": "", "pages": "57-64", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "Translational Proteomics", "DOI": "10.1016/j.trprot.2013.04.003", "citationKey": "", "url": "http://www.sciencedirect.com/science/article/pii/S2212963413000065", "accessDate": "2013-06-08T16:12:35Z", "PMID": "", "PMCID": "", "ISSN": "2212-9634", "archive": "", "archiveLocation": "", "shortTitle": "PanelomiX", "language": "", "libraryCatalog": "ScienceDirect", "callNumber": "", "rights": "", "extra": "", "tags": [ { "tag": "Aneurysmal subarachnoid haemorrhage", "type": 1 }, { "tag": "Biomarkers", "type": 1 }, { "tag": "Clinical study", "type": 1 }, { "tag": "Combination of biomarkers", "type": 1 }, { "tag": "Panel", "type": 1 }, { "tag": "machine learning", "type": 1 } ], "collections": [ "2UXWV7ZV", "75VPBAXC", "CNN5THP7", "E6BR434W", "NPRXC88X" ], "relations": {}, "dateAdded": "2013-06-08T16:17:55Z", "dateModified": "2013-06-15T15:14:28Z" } }, { "key": "QNBIB3VZ", "version": 68, "library": { "type": "group", "id": 62016, "name": "xavier.robin", "links": { "alternate": { "href": "https://www.zotero.org/groups/xavier.robin", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/62016/items/QNBIB3VZ", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/xavier.robin/items/QNBIB3VZ", "type": "text/html" } }, "meta": { "createdByUser": { "id": 12953, "username": "Calimo", "name": "Xavier Robin", "links": { "alternate": { "href": "https://www.zotero.org/calimo", "type": "text/html" } } }, "creatorSummary": "Tiberti et al.", "parsedDate": "2013", "numChildren": 0 }, "data": { "key": "QNBIB3VZ", "version": 68, "itemType": "journalArticle", "title": "Neopterin Is a Cerebrospinal Fluid Marker for Treatment Outcome Evaluation in Patients Affected by Trypanosoma brucei gambiense Sleeping Sickness", "creators": [ { "creatorType": "author", "firstName": "Natalia", "lastName": "Tiberti" }, { "creatorType": "author", "firstName": "Veerle", "lastName": "Lejon" }, { "creatorType": "author", "firstName": "Alexandre", "lastName": "Hainard" }, { "creatorType": "author", "firstName": "Bertrand", "lastName": "Courtioux" }, { "creatorType": "author", "firstName": "Xavier", "lastName": "Robin" }, { "creatorType": "author", "firstName": "Natacha", "lastName": "Turck" }, { "creatorType": "author", "firstName": "Krister", "lastName": "Kristensson" }, { "creatorType": "author", "firstName": "Enock", "lastName": "Matovu" }, { "creatorType": "author", "firstName": "John Charles", "lastName": "Enyaru" }, { "creatorType": "author", "firstName": "Dieudonné", "lastName": "Mumba Ngoyi" }, { "creatorType": "author", "firstName": "Sanjeev", "lastName": "Krishna" }, { "creatorType": "author", "firstName": "Sylvie", "lastName": "Bisser" }, { "creatorType": "author", "firstName": "Joseph Mathu", "lastName": "Ndung′u" }, { "creatorType": "author", "firstName": "Philippe", "lastName": "Büscher" }, { "creatorType": "author", "firstName": "Jean-Charles", "lastName": "Sanchez" } ], "abstractNote": "Author SummaryThe reduction of the number of lumbar punctures performed during the follow-up of patients affected by sleeping sickness (HAT) is considered a research priority. Follow-up, consisting of the examination of cerebrospinal fluid (CSF) for presence of parasites and for the number of leukocytes, is necessary to assess treatment outcome. However, diagnosis of treatment failure is still imperfect and WHO encourages improvements in defining criteria. Many studies have attempted to standardize actual methods and to define a cut-off for the number of white blood cells in CSF to define relapses, while only few have proposed alternatives to current practice. Here we show that neopterin, already proven to be a powerful marker for staging T. b. gambiense HAT, is also useful in evaluating post-therapeutic outcome. The measurement of neopterin concentration in CSF during the follow-up may allow reduction in the number of lumbar punctures from five to three for the majority of cured patients.", "publicationTitle": "PLOS Neglected Tropical Diseases", "publisher": "", "place": "", "date": "février 28, 2013", "volume": "7", "issue": "2", "section": "", "partNumber": "", "partTitle": "", "pages": "e2088", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "PLoS Negl Trop Dis", "DOI": "10.1371/journal.pntd.0002088", "citationKey": "", "url": "http://dx.doi.org/10.1371/journal.pntd.0002088", "accessDate": "2013-03-09T14:42:49Z", "PMID": "", "PMCID": "", "ISSN": "", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "", "libraryCatalog": "PLoS Negl Trop Dis", "callNumber": "", "rights": "", "extra": "PMID: 23469311", "tags": [], "collections": [ "4G5P8GBH", "75VPBAXC", "E6BR434W", "NPRXC88X", "TZNGZ92U", "XRTS6Q6R" ], "relations": {}, "dateAdded": "2013-03-09T14:43:34Z", "dateModified": "2013-03-09T14:52:32Z" } }, { "key": "E8AT7JWI", "version": 68, "library": { "type": "group", "id": 62016, "name": "xavier.robin", "links": { "alternate": { "href": "https://www.zotero.org/groups/xavier.robin", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/62016/items/E8AT7JWI", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/xavier.robin/items/E8AT7JWI", "type": "text/html" } }, "meta": { "createdByUser": { "id": 12953, "username": "Calimo", "name": "Xavier Robin", "links": { "alternate": { "href": "https://www.zotero.org/calimo", "type": "text/html" } } }, "creatorSummary": "Kennedy", "parsedDate": "2013", "numChildren": 0 }, "data": { "key": "E8AT7JWI", "version": 68, "itemType": "journalArticle", "title": "Clinical features, diagnosis, and treatment of human African trypanosomiasis (sleeping sickness)", "creators": [ { "creatorType": "author", "firstName": "Peter GE", "lastName": "Kennedy" } ], "abstractNote": "Summary \nHuman African trypanosomiasis, or sleeping sickness, is caused by infection with parasites of the genus Trypanosoma, transmitted by the tsetse fly. The disease has two forms, Trypanosoma brucei (T b) rhodesiense and T b gambiense; and is almost always fatal if untreated. Despite a recent reduction in the number of reported cases, patients with African trypanosomiasis continue to present major challenges to clinicians. Because treatment for CNS-stage disease can be very toxic, diagnostic staging to distinguish early-stage from late-stage disease when the CNS in invaded is crucial but remains problematic. Melarsoprol is the only available treatment for late-stage T b rhodesiense infection, but can be lethal to 5% of patients owing to post-treatment reactive encephalopathy. Eflornithine combined with nifurtimox is the first-line treatment for late-stage T b gambiense. New drugs are in the pipeline for treatment of CNS human African trypanosomiasis, giving rise to cautious optimism.", "publicationTitle": "The Lancet Neurology", "publisher": "", "place": "", "date": "février 2013", "volume": "12", "issue": "2", "section": "", "partNumber": "", "partTitle": "", "pages": "186-194", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "The Lancet Neurology", "DOI": "10.1016/S1474-4422(12)70296-X", "citationKey": "", "url": "http://www.sciencedirect.com/science/article/pii/S147444221270296X", "accessDate": "2013-03-02T07:52:31Z", "PMID": "", "PMCID": "", "ISSN": "1474-4422", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "", "libraryCatalog": "ScienceDirect", "callNumber": "", "rights": "", "extra": "", "tags": [], "collections": [ "E6BR434W", "XRTS6Q6R" ], "relations": {}, "dateAdded": "2013-03-02T07:53:42Z", "dateModified": "2013-03-02T07:53:42Z" } }, { "key": "22KZG98K", "version": 1, "library": { "type": "group", "id": 62016, "name": "xavier.robin", "links": { "alternate": { "href": "https://www.zotero.org/groups/xavier.robin", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/62016/items/22KZG98K", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/xavier.robin/items/22KZG98K", "type": "text/html" } }, "meta": { "createdByUser": { "id": 12953, "username": "Calimo", "name": "Xavier Robin", "links": { "alternate": { "href": "https://www.zotero.org/calimo", "type": "text/html" } } }, "creatorSummary": "MacLean et al.", "parsedDate": "2012-10-25", "numChildren": 0 }, "data": { "key": "22KZG98K", "version": 1, "itemType": "journalArticle", "title": "Stage Progression and Neurological Symptoms in Trypanosoma brucei rhodesiense Sleeping Sickness: Role of the CNS Inflammatory Response", "creators": [ { "creatorType": "author", "firstName": "Lorna", "lastName": "MacLean" }, { "creatorType": "author", "firstName": "Hansotto", "lastName": "Reiber" }, { "creatorType": "author", "firstName": "Peter G. E.", "lastName": "Kennedy" }, { "creatorType": "author", "firstName": "Jeremy M.", "lastName": "Sternberg" } ], "abstractNote": "Author SummaryHuman African trypanosomiasis, caused by the parasites Trypanosoma brucei rhodesiense and T.b.gambiense, is clinically defined by two diagnostic stages, an early stage where the parasites appear to be localised to the blood and lymphatic systems, and a late stage where the parasites are also localised in the central nervous system (CNS) and cause a meningoencephalitis, which is fatal if untreated. We studied the progression between these stages of infection in T.b.rhodesiense infections in Uganda. Progression from early to late stage was associated with an increase in inflammatory responses in the CNS as measured by analysis of the cerebrospinal fluid. However, contrary to predictions based on experimental model studies, neither disease stage progression nor CNS inflammatory responses were directly associated with development of neurological symptoms. Our results suggest that biological basis of the boundary between the two diagnostic stages in this infection may not be clear cut, with implications for therapeutic decision making.", "publicationTitle": "PLoS Negl Trop Dis", "publisher": "", "place": "", "date": "octobre 25, 2012", "volume": "6", "issue": "10", "section": "", "partNumber": "", "partTitle": "", "pages": "e1857", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "PLoS Negl Trop Dis", "DOI": "10.1371/journal.pntd.0001857", "citationKey": "", "url": "http://dx.doi.org/10.1371/journal.pntd.0001857", "accessDate": "2012-11-05T08:20:04Z", "PMID": "", "PMCID": "", "ISSN": "", "archive": "", "archiveLocation": "", "shortTitle": "Stage Progression and Neurological Symptoms in Trypanosoma brucei rhodesiense Sleeping Sickness", "language": "", "libraryCatalog": "PLoS Negl Trop Dis", "callNumber": "", "rights": "", "extra": "", "tags": [], "collections": [ "E6BR434W" ], "relations": {}, "dateAdded": "2012-11-05T08:20:19Z", "dateModified": "2012-11-05T08:20:19Z" } }, { "key": "ZKQ8BW72", "version": 1, "library": { "type": "group", "id": 62016, "name": "xavier.robin", "links": { "alternate": { "href": "https://www.zotero.org/groups/xavier.robin", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/62016/items/ZKQ8BW72", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/xavier.robin/items/ZKQ8BW72", "type": "text/html" } }, "meta": { "createdByUser": { "id": 12953, "username": "Calimo", "name": "Xavier Robin", "links": { "alternate": { "href": "https://www.zotero.org/calimo", "type": "text/html" } } }, "creatorSummary": "Turck et al.", "parsedDate": "2012-09-17", "numChildren": 0 }, "data": { "key": "ZKQ8BW72", "version": 1, "itemType": "journalArticle", "title": "Blood Glutathione S-Transferase-π as a Time Indicator of Stroke Onset", "creators": [ { "creatorType": "author", "firstName": "Natacha", "lastName": "Turck" }, { "creatorType": "author", "firstName": "Xavier", "lastName": "Robin" }, { "creatorType": "author", "firstName": "Nadia", "lastName": "Walter" }, { "creatorType": "author", "firstName": "Catherine", "lastName": "Fouda" }, { "creatorType": "author", "firstName": "Alexandre", "lastName": "Hainard" }, { "creatorType": "author", "firstName": "Roman", "lastName": "Sztajzel" }, { "creatorType": "author", "firstName": "Ghislaine", "lastName": "Wagner" }, { "creatorType": "author", "firstName": "Denis F.", "lastName": "Hochstrasser" }, { "creatorType": "author", "firstName": "Joan", "lastName": "Montaner" }, { "creatorType": "author", "firstName": "Pierre R.", "lastName": "Burkhard" }, { "creatorType": "author", "firstName": "Jean-Charles", "lastName": "Sanchez" } ], "abstractNote": "Background. Ability to accurately determine time of stroke onset remains challenging. We hypothesized that an early biomarker characterized by a rapid increase in blood after stroke onset may help defining better the time window during which an acute stroke patient may be candidate for intravenous thrombolysis or other intravascular procedures. \nMethods. The blood level of 29 proteins was measured by immunoassays on 2 independent and prospective cohorts of stroke patients (N=203) and controls (N=119). Mann-Whitney U tests, ROC curves and diagnostic odd ratios were applied to evaluate their clinical performances.\nResults. Among the 29 molecules tested in cohort 1, GST- concentration was the most significantly elevated marker in the blood of stroke patients (p<0.001). More importantly, GST- displayed the best area under the curve (AUC, 0.79) and the best diagnostic odd ratios (10.0) for discriminating early (within the first 3h of stroke onset) vs. late stroke patients (> 3h after onset). Moreover, GST- showed also the highest AUC (0.83) and performances for detecting patients treated with tPA (N=12) compared to ineligible patients (N=103). According to goal-oriented distinct cut-offs (sensitivity(Se)-oriented: 17.7 or specificity(Sp)-oriented: 65.2ug/L), the GST- test obtained 91%Se/50%Sp and 50%Se/91%Sp, respectively. Similar results were obtained in cohort 2. \nConclusions. This study demonstrates that GST-can accurately predict the time of stroke onset in over 50% of early stroke patients. The GST- test could therefore complement current guidelines for tPA administration and potentially increase the number of patients accessing thrombolysis.", "publicationTitle": "PLoS ONE", "publisher": "", "place": "", "date": "septembre 17, 2012", "volume": "7", "issue": "9", "section": "", "partNumber": "", "partTitle": "", "pages": "e43830", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "PLoS ONE", "DOI": "10.1371/journal.pone.0043830", "citationKey": "", "url": "http://dx.doi.org/10.1371/journal.pone.0043830", "accessDate": "2012-09-21T10:38:12Z", "PMID": "", "PMCID": "", "ISSN": "", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "", "libraryCatalog": "PLoS ONE", "callNumber": "", "rights": "", "extra": "", "tags": [], "collections": [ "2UXWV7ZV", "75VPBAXC", "E6BR434W", "NPRXC88X" ], "relations": {}, "dateAdded": "2012-09-21T16:20:16Z", "dateModified": "2012-09-21T16:20:16Z" } }, { "key": "7TZZXDPP", "version": 1, "library": { "type": "group", "id": 62016, "name": "xavier.robin", "links": { "alternate": { "href": "https://www.zotero.org/groups/xavier.robin", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/62016/items/7TZZXDPP", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/xavier.robin/items/7TZZXDPP", "type": "text/html" } }, "meta": { "createdByUser": { "id": 12953, "username": "Calimo", "name": "Xavier Robin", "links": { "alternate": { "href": "https://www.zotero.org/calimo", "type": "text/html" } } }, "creatorSummary": "Tiberti et al.", "parsedDate": "2012", "numChildren": 0 }, "data": { "key": "7TZZXDPP", "version": 1, "itemType": "journalArticle", "title": "Cerebrospinal Fluid Neopterin as Marker of the Meningo-Encephalitic Stage of Trypanosoma brucei gambiense Sleeping Sickness", "creators": [ { "creatorType": "author", "firstName": "Natalia", "lastName": "Tiberti" }, { "creatorType": "author", "firstName": "Alexandre", "lastName": "Hainard" }, { "creatorType": "author", "firstName": "Veerle", "lastName": "Lejon" }, { "creatorType": "author", "firstName": "Bertrand", "lastName": "Courtioux" }, { "creatorType": "author", "firstName": "Enock", "lastName": "Matovu" }, { "creatorType": "author", "firstName": "John Charles", "lastName": "Enyaru" }, { "creatorType": "author", "firstName": "Xavier", "lastName": "Robin" }, { "creatorType": "author", "firstName": "Natacha", "lastName": "Turck" }, { "creatorType": "author", "firstName": "Krister", "lastName": "Kristensson" }, { "creatorType": "author", "firstName": "Dieudonné Mumba", "lastName": "Ngoyi" }, { "creatorType": "author", "firstName": "Gedeão M. L.", "lastName": "Vatunga" }, { "creatorType": "author", "firstName": "Sanjeev", "lastName": "Krishna" }, { "creatorType": "author", "firstName": "Philippe", "lastName": "Büscher" }, { "creatorType": "author", "firstName": "Sylvie", "lastName": "Bisser" }, { "creatorType": "author", "firstName": "Joseph Mathu", "lastName": "Ndung’u" }, { "creatorType": "author", "firstName": "Jean-Charles", "lastName": "Sanchez" } ], "abstractNote": "BackgroundSleeping sickness, or human African trypanosomiasis (HAT), is a protozoan disease that affects rural communities in sub-Saharan Africa. Determination of the disease stage, essential for correct treatment, represents a key issue in the management of patients. In the present study we evaluated the potential of CXCL10, CXCL13, ICAM-1, VCAM-1, MMP-9, B2MG, neopterin and IgM to complement current methods for staging Trypanosoma brucei gambiense patients.Methods and FindingsFive hundred and twelve T. b. gambiense HAT patients originated from Angola, Chad and the Democratic Republic of the Congo (D.R.C.). Their classification as stage 2 (S2) was based on the number of white blood cells (WBC) (>5/µL) or presence of parasites in the cerebrospinal fluid (CSF). The CSF concentration of the eight markers was first measured on a training cohort encompassing 100 patients (44 S1 and 56 S2). IgM and neopterin were the best in discriminating between the two stages of disease with 86.4% and 84.1% specificity respectively, at 100% sensitivity. When a validation cohort (412 patients) was tested, neopterin (14.3 nmol/L) correctly classified 88% of S1 and S2 patients, confirming its high staging power. On this second cohort, neopterin also predicted both the presence of parasites, and of neurological signs, with the same ability as IgM and WBC, the current reference for staging.ConclusionsThis study has demonstrated that neopterin is an excellent biomarker for staging T. b. gambiense HAT patients. A rapid diagnostic test for detecting this metabolite in CSF could help in more accurate stage determination.", "publicationTitle": "PLoS ONE", "publisher": "", "place": "", "date": "juillet 18, 2012", "volume": "7", "issue": "7", "section": "", "partNumber": "", "partTitle": "", "pages": "e40909", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "PLoS ONE", "DOI": "10.1371/journal.pone.0040909", "citationKey": "", "url": "http://dx.doi.org/10.1371/journal.pone.0040909", "accessDate": "2012-07-23T06:55:58Z", "PMID": "", "PMCID": "", "ISSN": "", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "", "libraryCatalog": "PLoS ONE", "callNumber": "", "rights": "", "extra": "", "tags": [], "collections": [ "4G5P8GBH", "75VPBAXC", "E6BR434W", "NPRXC88X", "TZNGZ92U" ], "relations": {}, "dateAdded": "2012-07-23T07:00:59Z", "dateModified": "2012-07-23T07:00:59Z" } }, { "key": "TXW998ZP", "version": 1, "library": { "type": "group", "id": 62016, "name": "xavier.robin", "links": { "alternate": { "href": "https://www.zotero.org/groups/xavier.robin", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/62016/items/TXW998ZP", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/xavier.robin/items/TXW998ZP", "type": "text/html" } }, "meta": { "createdByUser": { "id": 12953, "username": "Calimo", "name": "Xavier Robin", "links": { "alternate": { "href": "https://www.zotero.org/calimo", "type": "text/html" } } }, "creatorSummary": "Masocha and Kristensson", "parsedDate": "2012-03-01", "numChildren": 0 }, "data": { "key": "TXW998ZP", "version": 1, "itemType": "journalArticle", "title": "Passage of parasites across the blood-brain barrier", "creators": [ { "creatorType": "author", "firstName": "Willias", "lastName": "Masocha" }, { "creatorType": "author", "firstName": "Krister", "lastName": "Kristensson" } ], "abstractNote": "The blood-brain barrier (BBB) is a structural and functional barrier that protects the central nervous system (CNS) from invasion by blood-borne pathogens including parasites. However, some intracellular and extracellular parasites can traverse the BBB during the course of infection and cause neurological disturbances and/or damage which are at times fatal. The means by which parasites cross the BBB and how the immune system controls the parasites within the brain are still unclear. In this review we present the current understanding of the processes utilized by two human neuropathogenic parasites, Trypanosoma brucei spp and Toxoplasma gondii, to go across the BBB and consequences of CNS invasion. We also describe briefly other parasites that can invade the brain and how they interact with or circumvent the BBB. The roles played by parasite-derived and host-derived molecules during parasitic and white blood cell invasion of the brain are discussed.", "publicationTitle": "Virulence", "publisher": "", "place": "", "date": "2012-03-01", "volume": "3", "issue": "2", "section": "", "partNumber": "", "partTitle": "", "pages": "192-202", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "", "DOI": "10.4161/viru.19178", "citationKey": "", "url": "http://apps.webofknowledge.com/InboundService.do?SID=S2onk1Iobd%40mAf79kdJ&product=WOS&UT=WOS%3A000305607700014&SrcApp=Alerting&DestFail=http%3A%2F%2Fwww.webofknowledge.com&Init=Yes&action=retrieve&Func=Frame&customersID=Alerting&SrcAuth=Alerting&IsProductCode=Yes&mode=FullRecord", "accessDate": "2012-07-12T07:11:03Z", "PMID": "", "PMCID": "", "ISSN": "2150-5594", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "", "libraryCatalog": "CrossRef", "callNumber": "", "rights": "", "extra": "", "tags": [], "collections": [ "4G5P8GBH", "E6BR434W" ], "relations": {}, "dateAdded": "2012-07-12T07:12:28Z", "dateModified": "2012-07-12T07:12:28Z" } }, { "key": "BUE4NSTT", "version": 1, "library": { "type": "group", "id": 62016, "name": "xavier.robin", "links": { "alternate": { "href": "https://www.zotero.org/groups/xavier.robin", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/62016/items/BUE4NSTT", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/xavier.robin/items/BUE4NSTT", "type": "text/html" } }, "meta": { "createdByUser": { "id": 12953, "username": "Calimo", "name": "Xavier Robin", "links": { "alternate": { "href": "https://www.zotero.org/calimo", "type": "text/html" } } }, "creatorSummary": "Bisser and Courtioux", "parsedDate": "2012", "numChildren": 0 }, "data": { "key": "BUE4NSTT", "version": 1, "itemType": "journalArticle", "title": "La maladie du sommeil, fin d’une épidémie ?", "creators": [ { "creatorType": "author", "firstName": "S.", "lastName": "Bisser" }, { "creatorType": "author", "firstName": "B.", "lastName": "Courtioux" } ], "abstractNote": "", "publicationTitle": "Revue Neurologique", "publisher": "", "place": "", "date": "3/2012", "volume": "168", "issue": "3", "section": "", "partNumber": "", "partTitle": "", "pages": "230-238", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "", "DOI": "10.1016/j.neurol.2011.12.004", "citationKey": "", "url": "http://www.em-consulte.com/article/702050/alertePM", "accessDate": "2012-05-18T06:57:34Z", "PMID": "", "PMCID": "", "ISSN": "00353787", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "fr", "libraryCatalog": "CrossRef", "callNumber": "", "rights": "", "extra": "", "tags": [], "collections": [ "4G5P8GBH", "E6BR434W" ], "relations": {}, "dateAdded": "2012-05-18T06:58:06Z", "dateModified": "2012-05-18T06:58:06Z" } }, { "key": "WX52J7ER", "version": 1, "library": { "type": "group", "id": 62016, "name": "xavier.robin", "links": { "alternate": { "href": "https://www.zotero.org/groups/xavier.robin", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/62016/items/WX52J7ER", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/xavier.robin/items/WX52J7ER", "type": "text/html" } }, "meta": { "createdByUser": { "id": 12953, "username": "Calimo", "name": "Xavier Robin", "links": { "alternate": { "href": "https://www.zotero.org/calimo", "type": "text/html" } } }, "creatorSummary": "Seke Etet and Fawzi Mahomoodally", "parsedDate": "2012", "numChildren": 0 }, "data": { "key": "WX52J7ER", "version": 1, "itemType": "journalArticle", "title": "New Insights in Staging and Chemotherapy of African Trypanosomiasis and Possible Contribution of Medicinal Plants", "creators": [ { "creatorType": "author", "firstName": "Paul F.", "lastName": "Seke Etet" }, { "creatorType": "author", "firstName": "M.", "lastName": "Fawzi Mahomoodally" } ], "abstractNote": "", "publicationTitle": "The Scientific World Journal", "publisher": "", "place": "", "date": "2012", "volume": "2012", "issue": "", "section": "", "partNumber": "", "partTitle": "", "pages": "1-16", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "", "DOI": "10.1100/2012/343652", "citationKey": "", "url": "http://www.tswj.com/2012/343652/", "accessDate": "2012-04-25T07:19:59Z", "PMID": "", "PMCID": "", "ISSN": "1537-744X", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "", "libraryCatalog": "CrossRef", "callNumber": "", "rights": "", "extra": "", "tags": [], "collections": [ "4G5P8GBH", "E6BR434W" ], "relations": {}, "dateAdded": "2012-04-25T07:21:09Z", "dateModified": "2012-04-25T07:21:09Z" } }, { "key": "WFCNNRWE", "version": 1, "library": { "type": "group", "id": 62016, "name": "xavier.robin", "links": { "alternate": { "href": "https://www.zotero.org/groups/xavier.robin", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/62016/items/WFCNNRWE", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/xavier.robin/items/WFCNNRWE", "type": "text/html" } }, "meta": { "createdByUser": { "id": 12953, "username": "Calimo", "name": "Xavier Robin", "links": { "alternate": { "href": "https://www.zotero.org/calimo", "type": "text/html" } } }, "creatorSummary": "Seke Etet et al.", "parsedDate": "2012-04-12", "numChildren": 0 }, "data": { "key": "WFCNNRWE", "version": 1, "itemType": "journalArticle", "title": "Sleep and Rhythm Changes at the Time of Trypanosoma brucei Invasion of the Brain Parenchyma in the Rat", "creators": [ { "creatorType": "author", "firstName": "Paul F.", "lastName": "Seke Etet" }, { "creatorType": "author", "firstName": "Maria", "lastName": "Palomba" }, { "creatorType": "author", "firstName": "Valeria", "lastName": "Colavito" }, { "creatorType": "author", "firstName": "Gigliola", "lastName": "Grassi-Zucconi" }, { "creatorType": "author", "firstName": "Marina", "lastName": "Bentivoglio" }, { "creatorType": "author", "firstName": "Giuseppe", "lastName": "Bertini" } ], "abstractNote": "Human African trypanosomiasis (HAT), or sleeping sickness, is a severe disease caused by Trypanosoma brucei (T.b.). The disease hallmark is sleep alterations. Brain involvement in HAT is a crucial pathogenetic step for disease diagnosis and therapy. In this study, a rat model of African trypanosomiasis was used to assess changes of sleep-wake, rest-activity, and body temperature rhythms in the time window previously shown as crucial for brain parenchyma invasion by T.b. to determine potential biomarkers of this event. Chronic radiotelemetric monitoring in Sprague-Dawley rats was used to continuously record electroencephalogram, electromyogram, rest-activity, and body temperature in the same animals before (baseline recording) and after infection. Rats were infected with T.b. brucei. Data were acquired from 1 to 20 d after infection (parasite neuroinvasion initiates at 11–13 d post-infection in this model), and were compared to baseline values. Sleep parameters were manually scored from electroencephalographic-electromyographic tracings. Circadian rhythms of sleep time, slow-wave activity, rest-activity, and body temperature were studied using cosinor rhythmometry. Results revealed alterations of most of the analyzed parameters. In particular, sleep pattern and sleep-wake organization plus rest-activity and body temperature rhythms exhibited early quantitative and qualitative alterations, which became marked around the time interval crucial for parasite neuroinvasion or shortly after. Data derived from actigrams showed close correspondence with those from hypnograms, suggesting that rest-activity could be useful to monitor sleep-wake alterations in African trypanosomiasis.", "publicationTitle": "Chronobiology International", "publisher": "", "place": "", "date": "2012-04-12", "volume": "", "issue": "", "section": "", "partNumber": "", "partTitle": "", "pages": "1-13", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "", "DOI": "10.3109/07420528.2012.660713", "citationKey": "", "url": "http://informahealthcare.com/doi/abs/10.3109/07420528.2012.660713", "accessDate": "2012-04-17T07:33:52Z", "PMID": "", "PMCID": "", "ISSN": "0742-0528, 1525-6073", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "", "libraryCatalog": "CrossRef", "callNumber": "", "rights": "", "extra": "", "tags": [], "collections": [ "4G5P8GBH", "E6BR434W" ], "relations": {}, "dateAdded": "2012-04-17T07:38:21Z", "dateModified": "2012-04-17T07:38:21Z" } } ]