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"filename": "De Groot et al. - 1994 - Characterization of type IV pilus genes in plant g.pdf", "md5": "f55e8ab3fc8ec4af20ebdfd49929687b", "mtime": 1706199236988, "tags": [], "relations": {}, "dateAdded": "2024-05-09T19:16:38Z", "dateModified": "2024-05-09T19:16:38Z" } }, { "key": "EW5MAVGW", "version": 774, "library": { "type": "group", "id": 45512, "name": "CSB/SJU Citation Manager", "links": { "alternate": { "href": "https://www.zotero.org/groups/csbsju_citation_manager", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/45512/items/EW5MAVGW", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/csbsju_citation_manager/items/EW5MAVGW", "type": "text/html" } }, "meta": { "createdByUser": { "id": 489765, "username": "jcarlson4", "name": "", "links": { "alternate": { "href": "https://www.zotero.org/jcarlson4", "type": "text/html" } } }, "creatorSummary": "De Groot et al.", "parsedDate": "1994", "numChildren": 2 }, "data": { "key": "EW5MAVGW", "version": 774, "itemType": "journalArticle", "title": "Characterization of type IV pilus genes in plant growth-promoting Pseudomonas putida WCS358", "creators": [ { "creatorType": "author", "firstName": "Arjan", "lastName": "De Groot" }, { "creatorType": "author", "firstName": "Ingmar", "lastName": "Heijnen" }, { "creatorType": "author", "firstName": "Hans", "lastName": "De Cock" }, { "creatorType": "author", "firstName": "Alain", "lastName": "Filloux" }, { "creatorType": "author", "firstName": "Jan", "lastName": "Tommassen" }, { "creatorType": "author", "firstName": "Jonathan", "lastName": "Carlson" } ], "abstractNote": "In a search for factors that could contribute to the ability of the plant growth-stimulating Pseudomonas putida WCS358 to colonize plant roots, the organism was analyzed for the presence of genes required for pilus biosynthesis. The pilD gene of Pseudomonas aeruginosa, which has also been designated xcpA, is involved in protein secretion and in the biogenesis of type IV pili. It encodes a peptidase that processes the precursors of the pilin subunits and of several components of the secretion apparatus. Prepilin processing activity could be demonstrated in P. putida WCS358, suggesting that this nonpathogenic strain may contain type IV pili as well. A DNA fragment containing the pilD (xcpA) gene of P. putida was cloned and found to complement a pilD (xcpA) mutation in P. aeruginosa. Nucleotide sequencing revealed, next to the pilD (xcpA) gene, the presence of two additional genes, piLA and pilC, that are highly homologous to genes involved in the biogenesis of type IV pili. The piLA gene encodes the pilin subunit, and pilC is an accessory gene, required for the assembly of the subunits into pili. In comparison with the pil gene cluster in P. aeruginosa, a gene homologous to pilB is lacking in the P. putida gene cluster. Pili were not detected on the cell surface of P. putida itself, not even when piLA was expressed from the tac promoter on a plasmid, indicating that not all the genes required for pilus biogenesis were expressed under the conditions tested. 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Put your summaries here.

\n
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The additional PV energy production, as well as the effect of non-evaporated water on the productivity of the hydropower plant, is analyzed by varying the basin surface coverage. The simulations highlight that the amount of additional hydroelectricity is quite small if compared to the non-FPV system, reaching about 3.56% for 25% basin surface coverage. However, the annual PV energy production is noticeable even at low coverage values. The expected gain in electricity production in the case of 25% basin surface coverage with the FPV plant rises to 391% of that of the actual hydropower plant. This gain becomes even larger if a vertical axis tracking system is installed and the increase is about 436%. The economic analysis confirms that the production costs (USD/kWh) of FPV systems are comparable to those of land-based PV (LBPV) plants, becoming smaller in the case that a tracking system is installed. In particular, the best solution is the one with 15% coverage of the lake. 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