Times Cited: 104\n
Note\n
English\n
Cited References Count: 41\n
EC722\n
1114 FIRST AVE, 4TH FL, NEW YORK, NY 10021\n
NEW YORK
", "tags": [], "relations": {}, "dateAdded": "2011-02-24T19:45:33Z", "dateModified": "2011-02-24T19:45:33Z" } }, { "key": "747PIPP7", "version": 1, "library": { "type": "group", "id": 39098, "name": "guilford_bead_size", "links": { "alternate": { "href": "https://www.zotero.org/groups/guilford_bead_size", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/39098/items/747PIPP7", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/guilford_bead_size/items/747PIPP7", "type": "text/html" } }, "meta": { "createdByUser": { "id": 190601, "username": "whg2n", "name": "Will Guilford", "links": { "alternate": { "href": "https://www.zotero.org/whg2n", "type": "text/html" } } }, "creatorSummary": "Bloodgood", "parsedDate": "1977", "numChildren": 1 }, "data": { "key": "747PIPP7", "version": 1, "itemType": "journalArticle", "title": "Motility occurring in association with surface of Chlamydomonas flagellum", "creators": [ { "creatorType": "author", "firstName": "R.A.", "lastName": "Bloodgood" } ], "abstractNote": "", "publicationTitle": "Journal of Cell Biology", "publisher": "", "place": "", "date": "1977", "volume": "75", "issue": "3", "section": "", "partNumber": "", "partTitle": "", "pages": "983-989", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "", "DOI": "", "citationKey": "", "url": "ISI:A1977EC72200026", "accessDate": "", "PMID": "", "PMCID": "", "ISSN": "", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "", "libraryCatalog": "", "callNumber": "", "rights": "", "extra": "", "tags": [ { "tag": "ASSOCIATION" }, { "tag": "Chlamydomonas" }, { "tag": "Flagella" }, { "tag": "SURFACE" }, { "tag": "motility" } ], "collections": [], "relations": {}, "dateAdded": "2011-02-24T19:45:33Z", "dateModified": "2011-02-24T19:45:33Z" } }, { "key": "NG2G8EVF", "version": 1, "library": { "type": "group", "id": 39098, "name": "guilford_bead_size", "links": { "alternate": { "href": "https://www.zotero.org/groups/guilford_bead_size", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/39098/items/NG2G8EVF", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/guilford_bead_size/items/NG2G8EVF", "type": "text/html" } }, "meta": { "createdByUser": { "id": 190601, "username": "whg2n", "name": "Will Guilford", "links": { "alternate": { "href": "https://www.zotero.org/whg2n", "type": "text/html" } } }, "creatorSummary": "Rasband", "parsedDate": "1997-01-01", "numChildren": 0 }, "data": { "key": "NG2G8EVF", "version": 1, "itemType": "book", "title": "NIH Image, ImageJ", "creators": [ { "creatorType": "author", "firstName": "W.S.", "lastName": "Rasband" } ], "abstractNote": "", "series": "", "seriesNumber": "", "volume": "", "numberOfVolumes": "", "edition": "", "date": "1997-2003 January 01, 1997", "publisher": "National Institutes of Health", "place": "Bethesda, Maryland", "originalDate": "", "originalPublisher": "", "originalPlace": "", "format": "", "numPages": "", "ISBN": "", "DOI": "", "citationKey": "", "url": "http://rsb.info.nih.gov/ij/", "accessDate": "", "ISSN": "", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "", "libraryCatalog": "", "callNumber": "", "rights": "", "extra": "", "tags": [], "collections": [], "relations": {}, "dateAdded": "2011-02-24T19:42:56Z", "dateModified": "2011-02-24T19:42:56Z" } }, { "key": "A44H7NIU", "version": 1, "library": { "type": "group", "id": 39098, "name": "guilford_bead_size", "links": { "alternate": { "href": "https://www.zotero.org/groups/guilford_bead_size", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/39098/items/A44H7NIU", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/guilford_bead_size/items/A44H7NIU", "type": "text/html" } }, "meta": { "createdByUser": { "id": 526192, "username": "bdhamankar", "name": "", "links": { "alternate": { "href": "https://www.zotero.org/bdhamankar", "type": "text/html" } } }, "creatorSummary": "Toba et al.", "parsedDate": "2006-04-11", "numChildren": 0 }, "data": { "key": "A44H7NIU", "version": 1, "itemType": "journalArticle", "title": "Overlapping hand-over-hand mechanism of single molecular motility of cytoplasmic dynein", "creators": [ { "creatorType": "author", "firstName": "Shiori", "lastName": "Toba" }, { "creatorType": "author", "firstName": "Tomonobu M.", "lastName": "Watanabe" }, { "creatorType": "author", "firstName": "Lisa", "lastName": "Yamaguchi-Okimoto" }, { "creatorType": "author", "firstName": "Yoko Yano", "lastName": "Toyoshima" }, { "creatorType": "author", "firstName": "Hideo", "lastName": "Higuchi" } ], "abstractNote": "Structural differences between dynein and kinesin suggest a unique molecular mechanism of dynein motility. Measuring the mechanical properties of a single molecule of dynein is crucial for revealing the mechanisms underlying its movement. We measured the step size and force produced by single molecules of active cytoplasmic dynein by using an optical trap and fluorescence imaging with a high temporal resolution. The velocity of dynein movement, 800 nm/s, is consistent with that reported in cells. The maximum force of 7–8 pN was independent of the ATP concentration and similar to that of kinesin. Dynein exhibited forward and occasional backwards steps of ≈8 nm, independent of load. It is suggested that the large dynein heads take 16-nm steps by using an overlapping hand-over-hand mechanism.", "publicationTitle": "Proceedings of the National Academy of Sciences", "publisher": "", "place": "", "date": "April 11 , 2006", "volume": "103", "issue": "15", "section": "", "partNumber": "", "partTitle": "", "pages": "5741 -5745", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "", "DOI": "10.1073/pnas.0508511103", "citationKey": "", "url": "http://www.pnas.org/content/103/15/5741.abstract", "accessDate": "2010-08-06T19:53:53Z", "PMID": "", "PMCID": "", "ISSN": "", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "", "libraryCatalog": "Highwire 2.0", "callNumber": "", "rights": "", "extra": "", "tags": [], "collections": [], "relations": {}, "dateAdded": "2011-02-23T19:56:52Z", "dateModified": "2011-02-23T19:56:52Z" } }, { "key": "98VX7RMG", "version": 1, "library": { "type": "group", "id": 39098, "name": "guilford_bead_size", "links": { "alternate": { "href": "https://www.zotero.org/groups/guilford_bead_size", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/39098/items/98VX7RMG", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/guilford_bead_size/items/98VX7RMG", "type": "text/html" } }, "meta": { "createdByUser": { "id": 526192, "username": "bdhamankar", "name": "", "links": { "alternate": { "href": "https://www.zotero.org/bdhamankar", "type": "text/html" } } }, "creatorSummary": "Svoboda and Block", "parsedDate": "1994", "numChildren": 0 }, "data": { "key": "98VX7RMG", "version": 1, "itemType": "journalArticle", "title": "Biological Applications of Optical Forces", "creators": [ { "creatorType": "author", "firstName": "K", "lastName": "Svoboda" }, { "creatorType": "author", "firstName": "S M", "lastName": "Block" } ], "abstractNote": "", "publicationTitle": "Annual Review of Biophysics and Biomolecular Structure", "publisher": "", "place": "", "date": "06/1994", "volume": "23", "issue": "1", "section": "", "partNumber": "", "partTitle": "", "pages": "247-285", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "Annu. Rev. Biophys. Biomol. Struct.", "DOI": "10.1146/annurev.bb.23.060194.001335", "citationKey": "", "url": "http://arjournals.annualreviews.org/doi/abs/10.1146/annurev.bb.23.060194.001335", "accessDate": "2010-08-09T21:34:18Z", "PMID": "", "PMCID": "", "ISSN": "1056-8700", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "", "libraryCatalog": "CrossRef", "callNumber": "", "rights": "", "extra": "", "tags": [], "collections": [], "relations": {}, "dateAdded": "2011-02-23T19:56:52Z", "dateModified": "2011-02-23T19:56:52Z" } }, { "key": "HDT9ERDK", "version": 1, "library": { "type": "group", "id": 39098, "name": "guilford_bead_size", "links": { "alternate": { "href": "https://www.zotero.org/groups/guilford_bead_size", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/39098/items/HDT9ERDK", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/guilford_bead_size/items/HDT9ERDK", "type": "text/html" } }, "meta": { "createdByUser": { "id": 526192, "username": "bdhamankar", "name": "", "links": { "alternate": { "href": "https://www.zotero.org/bdhamankar", "type": "text/html" } } }, "creatorSummary": "Rosenbaum and Witman", "parsedDate": "2002-11", "numChildren": 0 }, "data": { "key": "HDT9ERDK", "version": 1, "itemType": "journalArticle", "title": "Intraflagellar transport", "creators": [ { "creatorType": "author", "firstName": "Joel L.", "lastName": "Rosenbaum" }, { "creatorType": "author", "firstName": "George B.", "lastName": "Witman" } ], "abstractNote": "", "publicationTitle": "Nat Rev Mol Cell Biol", "publisher": "", "place": "", "date": "November 2002", "volume": "3", "issue": "11", "section": "", "partNumber": "", "partTitle": "", "pages": "813-825", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "Nat Rev Mol Cell Biol", "DOI": "10.1038/nrm952", "citationKey": "", "url": "http://dx.doi.org/10.1038/nrm952", "accessDate": "2010-09-23T02:36:00Z", "PMID": "", "PMCID": "", "ISSN": "1471-0072", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "", "libraryCatalog": "Nature", "callNumber": "", "rights": "", "extra": "", "tags": [], "collections": [], "relations": {}, "dateAdded": "2011-02-23T19:56:52Z", "dateModified": "2011-02-23T19:56:52Z" } }, { "key": "26BMP82C", "version": 1, "library": { "type": "group", "id": 39098, "name": "guilford_bead_size", "links": { "alternate": { "href": "https://www.zotero.org/groups/guilford_bead_size", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/39098/items/26BMP82C", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/guilford_bead_size/items/26BMP82C", "type": "text/html" } }, "meta": { "createdByUser": { "id": 526192, "username": "bdhamankar", "name": "", "links": { "alternate": { "href": "https://www.zotero.org/bdhamankar", "type": "text/html" } } }, "creatorSummary": "Neuman et al.", "parsedDate": "2005-06-01", "numChildren": 0 }, "data": { "key": "26BMP82C", "version": 1, "itemType": "journalArticle", "title": "Measurement of the effective focal shift in an optical trap", "creators": [ { "creatorType": "author", "firstName": "Keir C.", "lastName": "Neuman" }, { "creatorType": "author", "firstName": "Elio A.", "lastName": "Abbondanzieri" }, { "creatorType": "author", "firstName": "Steven M.", "lastName": "Block" } ], "abstractNote": "The focus of an oil-immersion microscope objective is shifted because of the refractive-index mismatch between the cover glass and the aqueous sample. We present a procedure with which to determine the focal shift by use of an inverted microscope equipped with optical tweezers. As the position of the sample chamber is scanned vertically, we measure the axial displacement of an optically trapped bead; the relative motion of the bead with respect to the surface supplies the effective focal shift. Measurements of this quantity deviate from electromagnetic calculations of the focal shift, a discrepancy attributable to the depth-dependent decrease in axial trap stiffness that arises from spherical aberration.", "publicationTitle": "Optics Letters", "publisher": "", "place": "", "date": "June 01, 2005", "volume": "30", "issue": "11", "section": "", "partNumber": "", "partTitle": "", "pages": "1318-1320", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "Opt. Lett.", "DOI": "10.1364/OL.30.001318", "citationKey": "", "url": "http://ol.osa.org/abstract.cfm?URI=ol-30-11-1318", "accessDate": "2010-08-09T21:29:32Z", "PMID": "", "PMCID": "", "ISSN": "", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "", "libraryCatalog": "Optical Society of America", "callNumber": "", "rights": "", "extra": "", "tags": [], "collections": [], "relations": {}, "dateAdded": "2011-02-23T19:56:52Z", "dateModified": "2011-02-23T19:56:52Z" } }, { "key": "XJ8FQ8JM", "version": 1, "library": { "type": "group", "id": 39098, "name": "guilford_bead_size", "links": { "alternate": { "href": "https://www.zotero.org/groups/guilford_bead_size", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/39098/items/XJ8FQ8JM", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/guilford_bead_size/items/XJ8FQ8JM", "type": "text/html" } }, "meta": { "createdByUser": { "id": 526192, "username": "bdhamankar", "name": "", "links": { "alternate": { "href": "https://www.zotero.org/bdhamankar", "type": "text/html" } } }, "creatorSummary": "Dupuis et al.", "parsedDate": "1997", "numChildren": 0 }, "data": { "key": "XJ8FQ8JM", "version": 1, "itemType": "journalArticle", "title": "Actin filament mechanics in the laser trap", "creators": [ { "creatorType": "author", "firstName": "DE", "lastName": "Dupuis" }, { "creatorType": "author", "firstName": "WH", "lastName": "Guilford" }, { "creatorType": "author", "firstName": "J", "lastName": "Wu" }, { "creatorType": "author", "firstName": "DM", "lastName": "Warshaw" } ], "abstractNote": "", "publicationTitle": "J Muscle Res Cell Motil", "publisher": "", "place": "", "date": "1997", "volume": "", "issue": "18", "section": "", "partNumber": "", "partTitle": "", "pages": "17-30", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "", "DOI": "", "citationKey": "", "url": "", "accessDate": "", "PMID": "", "PMCID": "", "ISSN": "", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "", "libraryCatalog": "", "callNumber": "", "rights": "", "extra": "", "tags": [ { "tag": "Bead Sizes" } ], "collections": [], "relations": {}, "dateAdded": "2011-02-23T19:56:52Z", "dateModified": "2011-02-23T19:56:52Z" } }, { "key": "9PUT8PJJ", "version": 1, "library": { "type": "group", "id": 39098, "name": "guilford_bead_size", "links": { "alternate": { "href": "https://www.zotero.org/groups/guilford_bead_size", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/39098/items/9PUT8PJJ", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/guilford_bead_size/items/9PUT8PJJ", "type": "text/html" } }, "meta": { "createdByUser": { "id": 526192, "username": "bdhamankar", "name": "", "links": { "alternate": { "href": "https://www.zotero.org/bdhamankar", "type": "text/html" } } }, "creatorSummary": "Bloodgood", "parsedDate": "1981", "numChildren": 0 }, "data": { "key": "9PUT8PJJ", "version": 1, "itemType": "journalArticle", "title": "Flagella-dependent gliding motility in Chlamydomonas", "creators": [ { "creatorType": "author", "firstName": "R. A.", "lastName": "Bloodgood" } ], "abstractNote": "Summary Flagella are generally recognized as organelles of motility responsible for the ability ofChlamydomonas to swim through its environment. However, the same flagella are also responsible for an alternative form of whole cell locomotion, termed gliding. Use of paralyzed flagella mutants demonstrates that gliding is independent of axonemal bend propagation. Gliding motility results from an interaction of the flagellar surface with a solid substrate. Gliding is characterized by bidirectional movements at 1.6±0.3 µm/second and occurs when the cell is in a characteristic gliding configuration, where the two flagella are oriented at 180° to one another. A variety of observations suggest that the leading flagellum is responsible for the force transduction resulting in cell locomotion, although both flagella have the capacity to function as the active flagellum. The characteristics of gliding motility have been compared with theChlamydomonas flagellar surface motility phenomenon defined as surface translocation of polystyrene microspheres.", "publicationTitle": "Protoplasma", "publisher": "", "place": "", "date": "1981", "volume": "106", "issue": "3", "section": "", "partNumber": "", "partTitle": "", "pages": "183-192", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "", "DOI": "10.1007/BF01275550", "citationKey": "", "url": "http://dx.doi.org/10.1007/BF01275550", "accessDate": "2010-08-06T19:00:01Z", "PMID": "", "PMCID": "", "ISSN": "", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "", "libraryCatalog": "SpringerLink", "callNumber": "", "rights": "", "extra": "", "tags": [ { "tag": "Bead Sizes" } ], "collections": [], "relations": {}, "dateAdded": "2011-02-23T19:56:52Z", "dateModified": "2011-02-23T19:56:52Z" } }, { "key": "W3T3CEE3", "version": 1, "library": { "type": "group", "id": 39098, "name": "guilford_bead_size", "links": { "alternate": { "href": "https://www.zotero.org/groups/guilford_bead_size", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/39098/items/W3T3CEE3", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/guilford_bead_size/items/W3T3CEE3", "type": "text/html" } }, "meta": { "createdByUser": { "id": 526192, "username": "bdhamankar", "name": "", "links": { "alternate": { "href": "https://www.zotero.org/bdhamankar", "type": "text/html" } } }, "creatorSummary": "Block et al.", "parsedDate": "2003-03-04", "numChildren": 0 }, "data": { "key": "W3T3CEE3", "version": 1, "itemType": "journalArticle", "title": "Probing the kinesin reaction cycle with a 2D optical force clamp", "creators": [ { "creatorType": "author", "firstName": "Steven M.", "lastName": "Block" }, { "creatorType": "author", "firstName": "Charles L.", "lastName": "Asbury" }, { "creatorType": "author", "firstName": "Joshua W.", "lastName": "Shaevitz" }, { "creatorType": "author", "firstName": "Matthew J.", "lastName": "Lang" } ], "abstractNote": "With every step it takes, the kinesin motor undergoes a mechanochemical reaction cycle that includes the hydrolysis of one ATP molecule, ADP/Pi release, plus an unknown number of additional transitions. Kinesin velocity depends on both the magnitude and the direction of the applied load. Using specialized apparatus, we subjected single kinesin molecules to forces in differing directions. Sideways and forward loads up to 8 pN exert only a weak effect, whereas comparable forces applied in the backward direction lead to stall. This strong directional bias suggests that the primary working stroke is closely aligned with the microtubule axis. Sideways loads slow the motor asymmetrically, but only at higher ATP levels, revealing the presence of additional, load-dependent transitions late in the cycle. Fluctuation analysis shows that the cycle contains at least four transitions, and confirms that hydrolysis remains tightly coupled to stepping. Together, our findings pose challenges for models of kinesin motion.", "publicationTitle": "Proceedings of the National Academy of Sciences of the United States of America", "publisher": "", "place": "", "date": "March 04 , 2003", "volume": "100", "issue": "5", "section": "", "partNumber": "", "partTitle": "", "pages": "2351 -2356", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "", "DOI": "10.1073/pnas.0436709100", "citationKey": "", "url": "http://www.pnas.org/content/100/5/2351.abstract", "accessDate": "2010-08-06T19:52:18Z", "PMID": "", "PMCID": "", "ISSN": "", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "", "libraryCatalog": "Highwire 2.0", "callNumber": "", "rights": "", "extra": "", "tags": [], "collections": [], "relations": {}, "dateAdded": "2011-02-23T19:56:52Z", "dateModified": "2011-02-23T19:56:52Z" } }, { "key": "6RCGNCWG", "version": 1, "library": { "type": "group", "id": 39098, "name": "guilford_bead_size", "links": { "alternate": { "href": "https://www.zotero.org/groups/guilford_bead_size", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/39098/items/6RCGNCWG", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/guilford_bead_size/items/6RCGNCWG", "type": "text/html" } }, "meta": { "createdByUser": { "id": 526192, "username": "bdhamankar", "name": "", "links": { "alternate": { "href": "https://www.zotero.org/bdhamankar", "type": "text/html" } } }, "creatorSummary": "Laib et al.", "parsedDate": "2009-03-03", "numChildren": 0 }, "data": { "key": "6RCGNCWG", "version": 1, "itemType": "journalArticle", "title": "The reciprocal coordination and mechanics of molecular motors in living cells", "creators": [ { "creatorType": "author", "firstName": "Jeneva A.", "lastName": "Laib" }, { "creatorType": "author", "firstName": "John A.", "lastName": "Marin" }, { "creatorType": "author", "firstName": "Robert A.", "lastName": "Bloodgood" }, { "creatorType": "author", "firstName": "William H.", "lastName": "Guilford" } ], "abstractNote": "Molecular motors in living cells are involved in whole-cell locomotion, contractility, developmental shape changes, and organelle movement and positioning. Whether motors of different directionality are functionally coordinated in cells or operate in a semirandom “tug of war” is unclear. We show here that anterograde and retrograde microtubule-based motors in the flagella of Chlamydomonas are regulated such that only motors of a common directionality are engaged at any single time. A laser trap was used to position microspheres on the plasma membrane of immobilized paralyzed Chlamydomonas flagella. The anterograde and retrograde movements of the microsphere were measured with nanometer resolution as microtubule-based motors engaged the transmembrane protein FMG-1. An average of 10 motors acted to move the microsphere in either direction. Reversal of direction during a transport event was uncommon, and quiescent periods separated every transport event, suggesting the coordinated and exclusive action of only a single motor type. After a jump to 32 °C, temperature-sensitive mutants of kinesin-2 (fla10) showed exclusively retrograde transport events, driven by 7 motors on average. These data suggest that molecular motors in living cells can be reciprocally coordinated to engage simultaneously in large numbers and for exclusive transport in a single direction, even when a mixed population of motors is present. This offers a unique model for studying the mechanics, regulation, and directional coordination of molecular motors in a living intracellular environment.", "publicationTitle": "Proceedings of the National Academy of Sciences", "publisher": "", "place": "", "date": "March 03 , 2009", "volume": "106", "issue": "9", "section": "", "partNumber": "", "partTitle": "", "pages": "3190 -3195", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "", "DOI": "10.1073/pnas.0809849106", "citationKey": "", "url": "http://www.pnas.org/content/106/9/3190.abstract", "accessDate": "2010-08-06T16:48:51Z", "PMID": "", "PMCID": "", "ISSN": "", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "", "libraryCatalog": "Highwire 2.0", "callNumber": "", "rights": "", "extra": "", "tags": [ { "tag": "Bead Sizes" } ], "collections": [], "relations": {}, "dateAdded": "2011-02-23T19:56:52Z", "dateModified": "2011-02-23T19:56:52Z" } }, { "key": "DI8ZSAV4", "version": 1, "library": { "type": "group", "id": 39098, "name": "guilford_bead_size", "links": { "alternate": { "href": "https://www.zotero.org/groups/guilford_bead_size", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/39098/items/DI8ZSAV4", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/guilford_bead_size/items/DI8ZSAV4", "type": "text/html" } }, "meta": { "createdByUser": { "id": 526192, "username": "bdhamankar", "name": "", "links": { "alternate": { "href": "https://www.zotero.org/bdhamankar", "type": "text/html" } } }, "creatorSummary": "Guilford et al.", "parsedDate": "2004", "numChildren": 0 }, "data": { "key": "DI8ZSAV4", "version": 1, "itemType": "journalArticle", "title": "Creating multiple time-shared laser traps with simultaneous displacement detection using digital signal processing hardware", "creators": [ { "creatorType": "author", "firstName": "WH", "lastName": "Guilford" }, { "creatorType": "author", "firstName": "JA", "lastName": "Tournas" }, { "creatorType": "author", "firstName": "D", "lastName": "Dascalu" }, { "creatorType": "author", "firstName": "DS", "lastName": "Watson" } ], "abstractNote": "", "publicationTitle": "Anal Biochem", "publisher": "", "place": "", "date": "2004", "volume": "", "issue": "326", "section": "", "partNumber": "", "partTitle": "", "pages": "153-166", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "", "DOI": "", "citationKey": "", "url": "http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6W9V-4BRP4J5-3&_user=709071&_coverDate=03%2F15%2F2004&_rdoc=1&_fmt=high&_orig=search&_sort=d&_docanchor=&view=c&_searchStrId=1423508126&_rerunOrigin=scholar.google&_acct=C000039638&_version=1&_urlVersion=0&_userid=709071&md5=84ca6a1ca95a4a810d232553f549acc3", "accessDate": "2010-08-06T19:34:59Z", "PMID": "", "PMCID": "", "ISSN": "", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "", "libraryCatalog": "", "callNumber": "", "rights": "", "extra": "", "tags": [], "collections": [], "relations": {}, "dateAdded": "2011-02-23T19:56:52Z", "dateModified": "2011-02-23T19:56:52Z" } }, { "key": "TZQWG2AZ", "version": 1, "library": { "type": "group", "id": 39098, "name": "guilford_bead_size", "links": { "alternate": { "href": "https://www.zotero.org/groups/guilford_bead_size", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/39098/items/TZQWG2AZ", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/guilford_bead_size/items/TZQWG2AZ", "type": "text/html" } }, "meta": { "createdByUser": { "id": 526192, "username": "bdhamankar", "name": "", "links": { "alternate": { "href": "https://www.zotero.org/bdhamankar", "type": "text/html" } } }, "creatorSummary": "Bloodgood and Workman", "parsedDate": "1984", "numChildren": 0 }, "data": { "key": "TZQWG2AZ", "version": 1, "itemType": "journalArticle", "title": "A flagellar surface glycoprotein mediating cell-substrate interaction in Chlamydomonas", "creators": [ { "creatorType": "author", "firstName": "Robert A.", "lastName": "Bloodgood" }, { "creatorType": "author", "firstName": "Lisa J.", "lastName": "Workman" } ], "abstractNote": "The Chlamydomonas flagellar surface exhibits interesting adhesive properties that are associated with flagellar surface motility. This dynamic surface property can be exhibited as the binding and movement of small polystyrene microspheres or as the interaction of the flagellar surface with a solid substrate followed by whole cell locomotion, termed ldquogliding.rdquo In order to identify flagellar surface proteins that mediate substrate interaction during flagellar surface motility, two immobilized iodination systems were employed that mimic the conditions for flagellar surface motility: small polystyrene microspheres derivatized with lactoperoxidase, and large glass beads derivatized with Iodogen. Use of these iodination conditions resulted in preferential iodination of a high-molecular-weight glycoprotein with apparent molecular weight of 300,000-350,000. These results suggest this glycoprotein as a major candidate for the surface-exposed adhesive component that directly interacts with the substrate and couples the substrate to a system of force transduction presumed to be located within the flagellum.", "publicationTitle": "Cell Motility and the Cytoskeleton", "publisher": "", "place": "", "date": "1984", "volume": "4", "issue": "2", "section": "", "partNumber": "", "partTitle": "", "pages": "77-87", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "", "DOI": "10.1002/cm.970040202", "citationKey": "", "url": "http://dx.doi.org/10.1002/cm.970040202", "accessDate": "2010-08-06T20:26:14Z", "PMID": "", "PMCID": "", "ISSN": "", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "", "libraryCatalog": "Wiley InterScience", "callNumber": "", "rights": "", "extra": "", "tags": [ { "tag": "Bead Sizes" } ], "collections": [], "relations": {}, "dateAdded": "2011-02-23T19:56:52Z", "dateModified": "2011-02-23T19:56:52Z" } }, { "key": "2U6VG2FP", "version": 1, "library": { "type": "group", "id": 39098, "name": "guilford_bead_size", "links": { "alternate": { "href": "https://www.zotero.org/groups/guilford_bead_size", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/39098/items/2U6VG2FP", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/guilford_bead_size/items/2U6VG2FP", "type": "text/html" } }, "meta": { "createdByUser": { "id": 526192, "username": "bdhamankar", "name": "", "links": { "alternate": { "href": "https://www.zotero.org/bdhamankar", "type": "text/html" } } }, "creatorSummary": "Bloodgood and Salomonsky", "parsedDate": "1998-03-01", "numChildren": 0 }, "data": { "key": "2U6VG2FP", "version": 1, "itemType": "journalArticle", "title": "Microsphere attachment induces glycoprotein redistribution and transmembrane signaling in theChlamydomonas flagellum", "creators": [ { "creatorType": "author", "firstName": "Robert A.", "lastName": "Bloodgood" }, { "creatorType": "author", "firstName": "Nancy L.", "lastName": "Salomonsky" } ], "abstractNote": "Summary The biflagellate green algaChlamydomonas can exhibit substrate-associated gliding motility in addition to its ability to swim through a liquid medium. The flagella are the organelles responsible for both forms of whole-cell locomotion although the mechanism in each case is very different. In this study, we demonstrate that the binding of polystyrene microspheres to the flagellar surface ofChlamydomonas initiates clustering of the major flagellar-membrane glycoprotein, which is known to be involved in motility-associated substrate adhesion. In addition, we demonstrate that microsphere binding to the flagellar surface initiates the same transmembrane signaling pathway that is initiated by antibody- or lectin-induced crosslinking of the major flagellar-membrane glycoprotein. In each case, the signaling pathway involves the activation of a calciumdependent protein phosphatase that dephosphorylates a flagellar phosphoprotein known to be associated with the major flagellar-membrane glycoprotein. Bound microspheres are translocated along the flagellar surface at approximately the same velocity as whole-cell gliding motility. Previous observations suggest that microsphere binding and translocation along the flagellar surface may be a reflection of the same force-transducing system responsible for whole-cell gliding motility. In which case, these observations suggest that the transmembrane signaling pathway initiated by crosslinking the major flagellar-membrane glycoprotein is the same one that is activated when the cell contacts a physiological substrate by its flagellar surface.", "publicationTitle": "Protoplasma", "publisher": "", "place": "", "date": "March 01, 1998", "volume": "202", "issue": "1", "section": "", "partNumber": "", "partTitle": "", "pages": "76-83", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "", "DOI": "10.1007/BF01280876", "citationKey": "", "url": "http://dx.doi.org/10.1007/BF01280876", "accessDate": "2010-08-06T20:39:54Z", "PMID": "", "PMCID": "", "ISSN": "", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "", "libraryCatalog": "SpringerLink", "callNumber": "", "rights": "", "extra": "", "tags": [], "collections": [], "relations": {}, "dateAdded": "2011-02-23T19:56:52Z", "dateModified": "2011-02-23T19:56:52Z" } }, { "key": "MFJ7RJGI", "version": 1, "library": { "type": "group", "id": 39098, "name": "guilford_bead_size", "links": { "alternate": { "href": "https://www.zotero.org/groups/guilford_bead_size", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/39098/items/MFJ7RJGI", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/guilford_bead_size/items/MFJ7RJGI", "type": "text/html" } }, "meta": { "createdByUser": { "id": 526192, "username": "bdhamankar", "name": "", "links": { "alternate": { "href": "https://www.zotero.org/bdhamankar", "type": "text/html" } } }, "creatorSummary": "Bloodgood and Salomonsky", "parsedDate": "1994", "numChildren": 0 }, "data": { "key": "MFJ7RJGI", "version": 1, "itemType": "journalArticle", "title": "The transmembrane signaling pathway involved in directed movements of Chlamydomonas flagellar membrane glycoproteins involves the dephosphorylation of a 60-kD phosphoprotein that binds to the major flagellar membrane glycoprotein", "creators": [ { "creatorType": "author", "firstName": "RA", "lastName": "Bloodgood" }, { "creatorType": "author", "firstName": "NL", "lastName": "Salomonsky" } ], "abstractNote": "", "publicationTitle": "J Cell Biol", "publisher": "", "place": "", "date": "1994", "volume": "", "issue": "127", "section": "", "partNumber": "", "partTitle": "", "pages": "803-811", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "", "DOI": "", "citationKey": "", "url": "", "accessDate": "", "PMID": "", "PMCID": "", "ISSN": "", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "", "libraryCatalog": "", "callNumber": "", "rights": "", "extra": "", "tags": [], "collections": [], "relations": {}, "dateAdded": "2011-02-23T19:56:52Z", "dateModified": "2011-02-23T19:56:52Z" } }, { "key": "PPU4X5XF", "version": 1, "library": { "type": "group", "id": 39098, "name": "guilford_bead_size", "links": { "alternate": { "href": "https://www.zotero.org/groups/guilford_bead_size", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/39098/items/PPU4X5XF", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/guilford_bead_size/items/PPU4X5XF", "type": "text/html" } }, "meta": { "createdByUser": { "id": 526192, "username": "bdhamankar", "name": "", "links": { "alternate": { "href": "https://www.zotero.org/bdhamankar", "type": "text/html" } } }, "creatorSummary": "Allersma et al.", "parsedDate": "1998-02", "numChildren": 0 }, "data": { "key": "PPU4X5XF", "version": 1, "itemType": "journalArticle", "title": "Two-Dimensional Tracking of ncd Motility by Back Focal Plane Interferometry", "creators": [ { "creatorType": "author", "firstName": "Miriam W.", "lastName": "Allersma" }, { "creatorType": "author", "firstName": "Frederick", "lastName": "Gittes" }, { "creatorType": "author", "firstName": "Michael J.", "lastName": "deCastro" }, { "creatorType": "author", "firstName": "Russell J.", "lastName": "Stewart" }, { "creatorType": "author", "firstName": "Christoph F.", "lastName": "Schmidt" } ], "abstractNote": "A technique for detecting the displacement of micron-sized optically trapped probes using far-field interference is introduced, theoretically explained, and used to study the motility of the ncd motor protein. Bead motions in the focal plane relative to the optical trap were detected by measuring laser intensity shifts in the back-focal plane of the microscope condenser by projection on a quadrant diode. This detection method is two-dimensional, largely independent of the position of the trap in the field of view and has ~10-[mu]s time resolution. The high resolution makes it possible to apply spectral analysis to measure dynamic parameters such as local viscosity and attachment compliance. A simple quantitative theory for back-focal-plane detection was derived that shows that the laser intensity shifts are caused primarily by a far-field interference effect. The theory predicts the detector response to bead displacement, without adjustable parameters, with good accuracy. To demonstrate the potential of the method, the ATP-dependent motility of ncd, a kinesin-related motor protein, was observed with an in vitro bead assay. A fusion protein consisting of truncated ncd (amino acids 195-685) fused with glutathione-S-transferase was adsorbed to silica beads, and the axial and lateral motions of the beads along the microtubule surface were observed with high spatial and temporal resolution. The average axial velocity of the ncd-coated beads was 230 ± 30 nm/s (average ± SD). Spectral analysis of bead motion showed the increase in viscous drag near the surface; we also found that any elastic constraints of the moving motors are much smaller than the constraints due to binding in the presence of the nonhydrolyzable nucleotide adenylylimidodiphosphate.", "publicationTitle": "Biophysical Journal", "publisher": "", "place": "", "date": "February 1998", "volume": "74", "issue": "2", "section": "", "partNumber": "", "partTitle": "", "pages": "1074-1085", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "", "DOI": "10.1016/S0006-3495(98)74031-7", "citationKey": "", "url": "http://www.sciencedirect.com/science/article/B94RW-4V295RH-1C/2/e61378ad23510eebe2a7afc4df48b32a", "accessDate": "2010-08-09T21:28:13Z", "PMID": "", "PMCID": "", "ISSN": "0006-3495", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "", "libraryCatalog": "ScienceDirect", "callNumber": "", "rights": "", "extra": "", "tags": [], "collections": [], "relations": {}, "dateAdded": "2011-02-23T19:56:52Z", "dateModified": "2011-02-23T19:56:52Z" } }, { "key": "T6CMRBHR", "version": 1, "library": { "type": "group", "id": 39098, "name": "guilford_bead_size", "links": { "alternate": { "href": "https://www.zotero.org/groups/guilford_bead_size", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/39098/items/T6CMRBHR", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/guilford_bead_size/items/T6CMRBHR", "type": "text/html" } }, "meta": { "createdByUser": { "id": 526192, "username": "bdhamankar", "name": "", "links": { "alternate": { "href": "https://www.zotero.org/bdhamankar", "type": "text/html" } } }, "creatorSummary": "Badoual et al.", "parsedDate": "2002-05-14", "numChildren": 0 }, "data": { "key": "T6CMRBHR", "version": 1, "itemType": "journalArticle", "title": "Bidirectional cooperative motion of molecular motors", "creators": [ { "creatorType": "author", "firstName": "M.", "lastName": "Badoual" }, { "creatorType": "author", "firstName": "F.", "lastName": "Jülicher" }, { "creatorType": "author", "firstName": "J.", "lastName": "Prost" } ], "abstractNote": "Recently, in a beautiful set of experiments, it has been shown that a Ncd mutant, NK11, which lacks directionality in its individual motion, was able to exhibit a new kind of directed motion in motility assays (Endow, S. A. & Higuchi, H. (2000) Nature (London) 406, 913–916): the filaments keep a given velocity for a while and then suddenly move in the opposite direction with similar velocity. We show that these observations nicely illustrate the concept of dynamic transitions in motor collections introduced earlier in the case of an infinite number of motors. We investigate the experimentally relevant case of a finite number of motors both when directionality is present (kinesins, myosins, Ncd) and absent (NK11). Using a symmetric two-state model, we demonstrate that bidirectional motion is the signature of a dynamic transition that results from the collective behavior of many motors acting on the same filament. For motors exhibiting directional bias individually, an asymmetric two-state model is appropriate. In that case, dynamic transitions exist for motor collections in the presence of an external force. We give predictions for the dependence of motion on ATP concentration, external forces, and the number of motors involved. In particular, we show that the reversal time grows exponentially with the number of motors per filament.", "publicationTitle": "Proceedings of the National Academy of Sciences of the United States of America", "publisher": "", "place": "", "date": "May 14 , 2002", "volume": "99", "issue": "10", "section": "", "partNumber": "", "partTitle": "", "pages": "6696 -6701", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "", "DOI": "10.1073/pnas.102692399", "citationKey": "", "url": "http://www.pnas.org/content/99/10/6696.abstract", "accessDate": "2010-08-06T20:41:30Z", "PMID": "", "PMCID": "", "ISSN": "", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "", "libraryCatalog": "Highwire 2.0", "callNumber": "", "rights": "", "extra": "", "tags": [ { "tag": "Bead Sizes" } ], "collections": [], "relations": {}, "dateAdded": "2011-02-23T19:56:45Z", "dateModified": "2011-02-23T19:56:45Z" } } ]