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The International Barcode of Life (iBOL) provides global coordination, but national nodes are essential for implementing barcoding at scale, building local capacity, and translating scientific advances into practice. This paper synthesizes experiences from 20 countries (17 in Europe), drawing on a survey and a workshop conducted under the Horizon Europe Biodiversity Genomics Europe project. We examine how national nodes are initiated, governed, and sustained, and identify common challenges such as defining scope, securing funding, harmonizing methods, and engaging stakeholders. Most nodes were initiated by research communities, and operate as informal networks with heterogeneous governance and staffing models. Key priorities include constructing comprehensive DNA barcode reference libraries, aligning activities with biomonitoring needs, and promoting FAIR and CARE data principles. We highlight strategies for capacity building, methodological standardization, and stakeholder engagement, alongside approaches for diversifying funding and strengthening communication. Based on these insights, we present ten practical recommendations to guide the establishment and long-term success of national DNA barcoding nodes. Strengthening these infrastructures will enhance Europe’s ability to deliver robust DNA-based biodiversity monitoring, underpin metabarcoding and metagenomic studies, and contribute to global efforts in species discovery, conservation, and environmental management.", "publicationTitle": "ARPHA Preprints", "publisher": "", "place": "", "date": "2026/01/08", "volume": "7", "issue": "", "section": "", "partNumber": "", "partTitle": "", "pages": "e184579", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "", "DOI": "10.3897/arphapreprints.e184579", "citationKey": "", "url": "https://preprints.arphahub.com/article/184579/", "accessDate": "2026-01-14T10:01:04Z", "PMID": "", "PMCID": "", "ISSN": "", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "en", "libraryCatalog": "preprints.arphahub.com", "callNumber": "", "rights": "2026 Elisavet Kaitetzidou, Piotr Gadawski, Will Goodall-Copestake, Gabriela Dankova, Konstantinos Gkagkavouzis, Sylwia Holak, Tomasz Rewicz, Karolina Bącela-Spychalska, Tomasz Mamos, Kasia Fantoni, Aleksandra Jabłońska, Grzegorz Tończyk, Łukasz Trębicki, Filippos Aravanopoulos, Claudia Bruschini, Georgi Bonchev, Magda Bou Dagher Kharrat, Fedor Čiampor Jr, Filipe Costa, Leonardo Dapporto, Torbjørn Ekrem, Sónia Ferreira, Matthias Geiger, Axel Hausmann, Paul Hebert, Belma Kalamujić Stroil, Stefaniya Kamenova, Ivona Kautmanova, Emre Keskin, Mladen Kučinić, Tatsiana Lipinskaya, Marko Mutanen, Spiros Papakostas, Ben Price, Rina Ramírez, Rodolphe Rougerie, Björn Rulik, Nikolaus Szucsich, Michelle van der Bank, Alexandros Triantafyllidis, Peter Hollingsworth, Michal Grabowski", "extra": "", "tags": [], "collections": [ "HRIG9SA7" ], "relations": {}, "dateAdded": "2026-01-14T10:10:12Z", "dateModified": "2026-01-14T10:10:12Z" } }, { "key": "HZDMWJKI", "version": 1003, "library": { "type": "group", "id": 369745, "name": "ABOL Literatur", "links": { "alternate": { "href": "https://www.zotero.org/groups/abol_literatur", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/369745/items/HZDMWJKI", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/abol_literatur/items/HZDMWJKI", "type": "text/html" }, "up": { "href": "https://api.zotero.org/groups/369745/items/HREAHBHF", "type": "application/json" } }, "meta": { "createdByUser": { "id": 2033903, "username": "abol", "name": "ABOL", "links": { "alternate": { "href": "https://www.zotero.org/abol", "type": "text/html" } } }, "numChildren": 0 }, "data": { "key": "HZDMWJKI", "version": 1003, "parentItem": "HREAHBHF", "itemType": "attachment", "linkMode": "imported_url", "title": "ScienceDirect Full Text PDF", "accessDate": "2025-12-31T11:28:56Z", "url": "https://www.sciencedirect.com/science/article/pii/S0378112725005122/pdfft?download=true", "note": "", "contentType": "application/pdf", "charset": "", "filename": "Jabot et al. - 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2025 - Use of massive DNA barcoding to monitor biodiversi.html", "md5": "76dd18a7e8c23654dae0a42cf0a232c7", "mtime": 1767180536552, "tags": [], "relations": {}, "dateAdded": "2025-12-31T11:31:16Z", "dateModified": "2025-12-31T11:31:16Z" } }, { "key": "HREAHBHF", "version": 1000, "library": { "type": "group", "id": 369745, "name": "ABOL Literatur", "links": { "alternate": { "href": "https://www.zotero.org/groups/abol_literatur", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/369745/items/HREAHBHF", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/abol_literatur/items/HREAHBHF", "type": "text/html" } }, "meta": { "createdByUser": { "id": 2033903, "username": "abol", "name": "ABOL", "links": { "alternate": { "href": "https://www.zotero.org/abol", "type": "text/html" } } }, "creatorSummary": "Jabot et al.", "parsedDate": "2025-11-01", "numChildren": 2 }, "data": { "key": "HREAHBHF", "version": 1000, "itemType": "journalArticle", "title": "Use of massive DNA barcoding to monitor biodiversity: A test on forest soil macrofauna", "creators": [ { "creatorType": "author", "firstName": "Franck", "lastName": "Jabot" }, { "creatorType": "author", "firstName": "Gwenaëlle", "lastName": "Auger" }, { "creatorType": "author", "firstName": "Pauline", "lastName": "Bonnal" }, { "creatorType": "author", "firstName": "Mathilde", "lastName": "Pizaine" }, { "creatorType": "author", "firstName": "Marilyn", "lastName": "Roncoroni" }, { "creatorType": "author", "firstName": "Sandrine", "lastName": "Revaillot" }, { "creatorType": "author", "firstName": "Julien", "lastName": "Pottier" } ], "abstractNote": "Biodiversity monitoring primarily focuses on particular taxonomic groups for which identification expertise is widely available, such as vascular plants or birds. As a result, the response of many taxonomic groups to forest management is much less documented. With the advent of low-cost next generation barcoding approaches, it is now possible to envisage monitoring strategies based on molecular approaches, beyond metabarcoding/eDNA strategies that do not give access to species abundances and integrate species presence over largely unknown spatio-temporal scales. In this contribution, we demonstrate the use of massive DNA barcoding – also referred as megabarcoding – as a promising solution to overcome identification difficulties in demanding taxonomic groups. We performed a proof of concept study in a mountainous beech forest in the Massif Central, France. We sampled soil macrofauna at 25 sampling sites and managed to visually identify at the species level 130 out of the 1413 sampled individuals. Using megabarcoding on the 1283 remaining non-identified individuals, we managed to assign 1124 additional individuals to an operational taxonomic species at a competitive cost. We present the summary statistics of barcoding success in the different taxonomic groups encountered and in larvae versus adult individuals. We demonstrate that larvae individuals, which can hardly be visually identified at the species level, make a substantial contribution to overall macrofauna diversity. We finally showcase how this megabarcoding approach provides a concrete avenue for forest biodiversity monitoring, by assessing the cost and labour intensity of this approach.", "publicationTitle": "Forest Ecology and Management", "publisher": "", "place": "", "date": "2025-11-01", "volume": "595", "issue": "", "section": "", "partNumber": "", "partTitle": "", "pages": "123004", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "Forest Ecology and Management", "DOI": "10.1016/j.foreco.2025.123004", "citationKey": "", "url": "https://www.sciencedirect.com/science/article/pii/S0378112725005122", "accessDate": "2025-12-31T11:28:44Z", "PMID": "", "PMCID": "", "ISSN": "0378-1127", "archive": "", "archiveLocation": "", "shortTitle": "Use of massive DNA barcoding to monitor biodiversity", "language": "", "libraryCatalog": "ScienceDirect", "callNumber": "", "rights": "", "extra": "", "tags": [ { "tag": "Biodiversity", "type": 1 }, { "tag": "DNA barcoding", "type": 1 }, { "tag": "Megabarcoding", "type": 1 }, { "tag": "Monitoring", "type": 1 }, { "tag": "Soil fauna", "type": 1 } ], "collections": [ "74H4DIHW" ], "relations": {}, "dateAdded": "2025-12-31T11:31:15Z", "dateModified": "2025-12-31T11:31:15Z" } }, { "key": "9BZVWCQB", "version": 999, "library": { "type": "group", "id": 369745, "name": "ABOL Literatur", "links": { "alternate": { "href": "https://www.zotero.org/groups/abol_literatur", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/369745/items/9BZVWCQB", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/abol_literatur/items/9BZVWCQB", "type": "text/html" }, "up": { "href": "https://api.zotero.org/groups/369745/items/PZA44CIP", "type": "application/json" } }, "meta": { "createdByUser": { "id": 2033903, "username": "abol", "name": "ABOL", "links": { "alternate": { "href": "https://www.zotero.org/abol", "type": "text/html" } } }, "numChildren": 0 }, "data": { "key": "9BZVWCQB", "version": 999, "parentItem": "PZA44CIP", "itemType": "attachment", "linkMode": "imported_url", "title": "Full Text PDF", "accessDate": "2025-11-10T10:37:53Z", "url": "https://www.nature.com/articles/s44185-025-00108-3.pdf", "note": "", "contentType": "application/pdf", "charset": "", "filename": "Lehmitz et al. - 2025 - Unknown Germany - An integrative biodiversity disc.pdf", "md5": "2d5771eb01dc0bec50b7b9279175c7d9", "mtime": 1762771073806, "tags": [], "relations": {}, "dateAdded": "2025-11-10T10:56:54Z", "dateModified": "2025-11-10T10:56:54Z" } }, { "key": "PZA44CIP", "version": 997, "library": { "type": "group", "id": 369745, "name": "ABOL Literatur", "links": { "alternate": { "href": "https://www.zotero.org/groups/abol_literatur", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/369745/items/PZA44CIP", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/abol_literatur/items/PZA44CIP", "type": "text/html" } }, "meta": { "createdByUser": { "id": 2033903, "username": "abol", "name": "ABOL", "links": { "alternate": { "href": "https://www.zotero.org/abol", "type": "text/html" } } }, "creatorSummary": "Lehmitz et al.", "parsedDate": "2025-11-05", "numChildren": 1 }, "data": { "key": "PZA44CIP", "version": 997, "itemType": "journalArticle", "title": "Unknown Germany - An integrative biodiversity discovery program", "creators": [ { "creatorType": "author", "firstName": "Ricarda", "lastName": "Lehmitz" }, { "creatorType": "author", "firstName": "Karin", "lastName": "Hohberg" }, { "creatorType": "author", "firstName": "Martin", "lastName": "Husemann" }, { "creatorType": "author", "firstName": "Ralph S.", "lastName": "Peters" }, { "creatorType": "author", "firstName": "Nicole", "lastName": "Scheunemann" }, { "creatorType": "author", "firstName": "Miklós", "lastName": "Bálint" }, { "creatorType": "author", "firstName": "Michael", "lastName": "Balke" }, { "creatorType": "author", "firstName": "Judith", "lastName": "Bieberich" }, { "creatorType": "author", "firstName": "Iliana", "lastName": "Bista" }, { "creatorType": "author", "firstName": "Thomas", "lastName": "Borsch" }, { "creatorType": "author", "firstName": "Sarah J.", "lastName": "Bourlat" }, { "creatorType": "author", "firstName": "Rainer W.", "lastName": "Bussmann" }, { "creatorType": "author", "firstName": "Ulrike", "lastName": "Damm" }, { "creatorType": "author", "firstName": "Lara-Sophie", "lastName": "Dey" }, { "creatorType": "author", "firstName": "Cecilia G.", "lastName": "Flocco" }, { "creatorType": "author", "firstName": "Hans-Peter", "lastName": "Grossart" }, { "creatorType": "author", "firstName": "Peter", "lastName": "Haase" }, { "creatorType": "author", "firstName": "Hubert", "lastName": "Höfer" }, { "creatorType": "author", "firstName": "Valentyna", "lastName": "Krashevska" }, { "creatorType": "author", "firstName": "Lars", "lastName": "Krogmann" }, { "creatorType": "author", "firstName": "Robert", "lastName": "Lücking" }, { "creatorType": "author", "firstName": "Pedro Martinez", "lastName": "Arbizu" }, { "creatorType": "author", "firstName": "Rudolf", "lastName": "Meier" }, { "creatorType": "author", "firstName": "Ximo", "lastName": "Mengual" }, { "creatorType": "author", "firstName": "Nancy F.", "lastName": "Mercado-Salas" }, { "creatorType": "author", "firstName": "Jenna M.", "lastName": "Moore" }, { "creatorType": "author", "firstName": "Michael C.", "lastName": "Orr" }, { "creatorType": "author", "firstName": "Volker", "lastName": "Otte" }, { "creatorType": "author", "firstName": "Jörg", "lastName": "Overmann" }, { "creatorType": "author", "firstName": "Steffen U.", "lastName": "Pauls" }, { "creatorType": "author", "firstName": "Ricardo J.", "lastName": "Pereira" }, { "creatorType": "author", "firstName": "Joris", "lastName": "Peters" }, { "creatorType": "author", "firstName": "Anton", "lastName": "Potapov" }, { "creatorType": "author", "firstName": "Christian", "lastName": "Printzen" }, { "creatorType": "author", "firstName": "Florian", "lastName": "Raub" }, { "creatorType": "author", "firstName": "Michael J.", "lastName": "Raupach" }, { "creatorType": "author", "firstName": "Vera", "lastName": "Rduch" }, { "creatorType": "author", "firstName": "Alice", "lastName": "Retter" }, { "creatorType": "author", "firstName": "Björn M.", "lastName": "von Reumont" }, { "creatorType": "author", "firstName": "Sven", "lastName": "Rossel" }, { "creatorType": "author", "firstName": "Rüdiger M.", "lastName": "Schmelz" }, { "creatorType": "author", "firstName": "Thomas", "lastName": "Schmitt" }, { "creatorType": "author", "firstName": "Markus", "lastName": "Scholler" }, { "creatorType": "author", "firstName": "Eckart", "lastName": "Stolle" }, { "creatorType": "author", "firstName": "Marco", "lastName": "Thines" }, { "creatorType": "author", "firstName": "Cristina", "lastName": "Vasilita" }, { "creatorType": "author", "firstName": "Thomas", "lastName": "Wesener" }, { "creatorType": "author", "firstName": "Reza", "lastName": "Zahiri" }, { "creatorType": "author", "firstName": "Andrey", "lastName": "Zaitsev" }, { "creatorType": "author", "firstName": "Jonas", "lastName": "Zimmermann" }, { "creatorType": "author", "firstName": "Dagmara", "lastName": "Żyła" }, { "creatorType": "author", "firstName": "Bernhard", "lastName": "Misof" }, { "creatorType": "author", "firstName": "Klement", "lastName": "Tockner" } ], "abstractNote": "Biodiversity knowledge, from genes to ecosystems, is crucial for addressing the biodiversity crisis. However, even in well-explored countries like Germany, much biodiversity remains unknown. Therefore, several research institutions are joining forces to conduct a comprehensive biodiversity inventory, combining broad taxonomic expertise with advanced technologies. By consolidating data across many organismic groups, the Unknown Germany initiative will significantly enhance conservation strategies and may serve as a model for similar efforts worldwide.", "publicationTitle": "npj Biodiversity", "publisher": "", "place": "", "date": "2025-11-05", "volume": "4", "issue": "1", "section": "", "partNumber": "", "partTitle": "", "pages": "41", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "npj biodivers", "DOI": "10.1038/s44185-025-00108-3", "citationKey": "", "url": "https://www.nature.com/articles/s44185-025-00108-3", "accessDate": "2025-11-10T10:37:52Z", "PMID": "", "PMCID": "", "ISSN": "2731-4243", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "en", "libraryCatalog": "www.nature.com", "callNumber": "", "rights": "2025 The Author(s)", "extra": "", "tags": [ { "tag": "Biodiversity", "type": 1 }, { "tag": "Conservation biology", "type": 1 } ], "collections": [ "DUI7UNXL" ], "relations": {}, "dateAdded": "2025-11-10T10:56:53Z", "dateModified": "2025-11-10T10:56:53Z" } }, { "key": "CQHINRBS", "version": 996, "library": { "type": "group", "id": 369745, "name": "ABOL Literatur", "links": { "alternate": { "href": "https://www.zotero.org/groups/abol_literatur", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/369745/items/CQHINRBS", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/abol_literatur/items/CQHINRBS", "type": "text/html" }, "up": { "href": "https://api.zotero.org/groups/369745/items/5AU74VCU", "type": "application/json" } }, "meta": { "createdByUser": { "id": 2033903, "username": "abol", "name": "ABOL", "links": { "alternate": { "href": "https://www.zotero.org/abol", "type": "text/html" } } }, "lastModifiedByUser": { "id": 1061585, "username": "nhm_vienna_crl", "name": "", "links": { "alternate": { "href": "https://www.zotero.org/nhm_vienna_crl", "type": "text/html" } } } }, "data": { "key": "CQHINRBS", "version": 996, "parentItem": "5AU74VCU", "itemType": "attachment", "linkMode": "imported_url", "title": "ScienceDirect Snapshot", "accessDate": "2025-11-03T14:13:38Z", "url": "https://www.sciencedirect.com/science/article/pii/S0043135424018876", "note": "", "contentType": "text/html", "charset": "", "filename": "Macher et al. - 2025 - Fit for purpose Evaluating benthic invertebrate D.html", "md5": "7e57a749f910126e8c35e87680ad7384", "mtime": 1762179218915, "tags": [], "relations": {}, "dateAdded": "2025-11-03T14:14:46Z", "dateModified": "2025-11-03T14:14:46Z" } }, { "key": "5KREN3QP", "version": 996, "library": { "type": "group", "id": 369745, "name": "ABOL Literatur", "links": { "alternate": { "href": "https://www.zotero.org/groups/abol_literatur", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/369745/items/5KREN3QP", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/abol_literatur/items/5KREN3QP", "type": "text/html" }, "up": { "href": "https://api.zotero.org/groups/369745/items/5AU74VCU", "type": "application/json" } }, "meta": { "createdByUser": { "id": 2033903, "username": "abol", "name": "ABOL", "links": { "alternate": { "href": "https://www.zotero.org/abol", "type": "text/html" } } }, "lastModifiedByUser": { "id": 1061585, "username": "nhm_vienna_crl", "name": "", "links": { "alternate": { "href": "https://www.zotero.org/nhm_vienna_crl", "type": "text/html" } } }, "numChildren": 0 }, "data": { "key": "5KREN3QP", "version": 996, "parentItem": "5AU74VCU", "itemType": "attachment", "linkMode": "imported_url", "title": "ScienceDirect Full Text PDF", "accessDate": "2025-11-03T14:13:38Z", "url": "https://www.sciencedirect.com/science/article/pii/S0043135424018876/pdfft?download=true", "note": "", "contentType": "application/pdf", "charset": "", "filename": "Macher et al. - 2025 - Fit for purpose Evaluating benthic invertebrate D.pdf", "md5": "ac0fcbfc87b7be953d32ffc0cdbc623a", "mtime": 1762179226053, "tags": [], "relations": {}, "dateAdded": "2025-11-03T14:14:45Z", "dateModified": "2025-11-03T14:14:45Z" } }, { "key": "5AU74VCU", "version": 992, "library": { "type": "group", "id": 369745, "name": "ABOL Literatur", "links": { "alternate": { "href": "https://www.zotero.org/groups/abol_literatur", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/369745/items/5AU74VCU", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/abol_literatur/items/5AU74VCU", "type": "text/html" } }, "meta": { "createdByUser": { "id": 2033903, "username": "abol", "name": "ABOL", "links": { "alternate": { "href": "https://www.zotero.org/abol", "type": "text/html" } } }, "creatorSummary": "Macher et al.", "parsedDate": "2025-03-15", "numChildren": 2 }, "data": { "key": "5AU74VCU", "version": 992, "itemType": "journalArticle", "title": "Fit for purpose? Evaluating benthic invertebrate DNA metabarcoding for ecological status class assessment in streams under the Water Framework Directive", "creators": [ { "creatorType": "author", "firstName": "Till-Hendrik", "lastName": "Macher" }, { "creatorType": "author", "firstName": "Arne J.", "lastName": "Beermann" }, { "creatorType": "author", "firstName": "Jens", "lastName": "Arle" }, { "creatorType": "author", "firstName": "Julia", "lastName": "Foerster" }, { "creatorType": "author", "firstName": "Matthias", "lastName": "Greyer" }, { "creatorType": "author", "firstName": "Demetrio", "lastName": "Mora" }, { "creatorType": "author", "firstName": "Jan", "lastName": "Koschorreck" }, { "creatorType": "author", "firstName": "Peter", "lastName": "Rolauffs" }, { "creatorType": "author", "firstName": "Anne", "lastName": "Rother" }, { "creatorType": "author", "firstName": "Susanne", "lastName": "Schüler" }, { "creatorType": "author", "firstName": "Jonas", "lastName": "Zimmermann" }, { "creatorType": "author", "firstName": "Daniel", "lastName": "Hering" }, { "creatorType": "author", "firstName": "Florian", "lastName": "Leese" } ], "abstractNote": "The ecological state of aquatic ecosystems is systematically monitored using various bioindicators in many countries worldwide. In the European Union, freshwater biomonitoring is the central component of the EU Water Framework Directive (WFD, 2000/60/EC) and currently based on morpho-taxonomic methods. DNA metabarcoding is a novel approach to assess the ecological state fast and efficiently based on organismal DNA signatures and thereby support and upscale biomonitoring. However, compliance of metabarcoding with existing morpho-taxonomic methods must be ensured prior to official implementation. Thus, this study, co-designed by research institutions and environmental agencies, explored necessary key parameters and performed method intercalibration for the implementation of metabarcoding into WFD assessments of running waters. We focussed on benthic invertebrates as the most commonly used bioindicators. We analysed 170 invertebrate samples collected as part of the German federal state WFD routine stream biomonitoring, first via microscopic determination and then using metabarcoding. Our goals were to quantify overlap in i) taxonomic composition and ii) ecological status derived with both methods. For this purpose, we established data harmonisation measures to integrate invertebrate metabarcoding data into the official national WFD classification modules considering abundance and presence/absence data. Our results revealed a high (ca. 70 %) overlap of bioindicator taxa found with both methods. Metabarcoding identified significantly more small invertebrate taxa and detected similar proportions of the important bioindicator ‘EPT’ taxa (mayflies, stoneflies, caddisflies). Despite deviations in some detected bioindicator taxa, the derived ecological status classes were highly correlated between methods, particularly after intercalibration (R2 = 0.74, Spearman rho = 0.86). Regardless of whether we used abundance or presence/absence data, the resulting stream type classifications showed strong agreement. Thus, our study not only demonstrates the consistency of the methods for the stream types analysed but is also the first to operationalise a path to integration of metabarcoding data into the WFD assessment modules based on formal intercalibration guidelines.", "publicationTitle": "Water Research", "publisher": "", "place": "", "date": "2025-03-15", "volume": "272", "issue": "", "section": "", "partNumber": "", "partTitle": "", "pages": "122987", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "Water Research", "DOI": "10.1016/j.watres.2024.122987", "citationKey": "", "url": "https://www.sciencedirect.com/science/article/pii/S0043135424018876", "accessDate": "2025-11-03T14:13:29Z", "PMID": "", "PMCID": "", "ISSN": "0043-1354", "archive": "", "archiveLocation": "", "shortTitle": "Fit for purpose?", "language": "", "libraryCatalog": "ScienceDirect", "callNumber": "", "rights": "", "extra": "", "tags": [ { "tag": "Bioassessment", "type": 1 }, { "tag": "Biomonitoring", "type": 1 }, { "tag": "Regulation", "type": 1 }, { "tag": "Rivers", "type": 1 } ], "collections": [ "PZDJ5JA3" ], "relations": {}, "dateAdded": "2025-11-03T14:14:45Z", "dateModified": "2025-11-03T14:14:45Z" } }, { "key": "UPSFEBHU", "version": 991, "library": { "type": "group", "id": 369745, "name": "ABOL Literatur", "links": { "alternate": { "href": "https://www.zotero.org/groups/abol_literatur", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/369745/items/UPSFEBHU", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/abol_literatur/items/UPSFEBHU", "type": "text/html" } }, "meta": { "createdByUser": { "id": 2033903, "username": "abol", "name": "ABOL", "links": { "alternate": { "href": "https://www.zotero.org/abol", "type": "text/html" } } }, "creatorSummary": "Jones et al.", "parsedDate": "2025", "numChildren": 0 }, "data": { "key": "UPSFEBHU", "version": 991, "itemType": "journalArticle", "title": "Sources of uncertainty in DNA metabarcoding of whole communities: Implications for its use in biomonitoring", "creators": [ { "creatorType": "author", "firstName": "J. Iwan", "lastName": "Jones" }, { "creatorType": "author", "firstName": "Amanda", "lastName": "Arnold" }, { "creatorType": "author", "firstName": "Dominik", "lastName": "Buchner" }, { "creatorType": "author", "firstName": "John F.", "lastName": "Murphy" }, { "creatorType": "author", "firstName": "Jasmine N.", "lastName": "Childress" }, { "creatorType": "author", "firstName": "Pedro", "lastName": "Beja" }, { "creatorType": "author", "firstName": "Florian", "lastName": "Altermatt" }, { "creatorType": "author", "firstName": "Kristian", "lastName": "Meissner" }, { "creatorType": "author", "firstName": "Ian", "lastName": "Adams" }, { "creatorType": "author", "firstName": "Meri", "lastName": "Anderson" }, { "creatorType": "author", "firstName": "Yaisel J.", "lastName": "Borrell" }, { "creatorType": "author", "firstName": "Andrew G.", "lastName": "Briscoe" }, { "creatorType": "author", "firstName": "Kat", "lastName": "Bruce" }, { "creatorType": "author", "firstName": "Cátia", "lastName": "Chaves" }, { "creatorType": "author", "firstName": "Chris", "lastName": "Conyers" }, { "creatorType": "author", "firstName": "Kristy", "lastName": "Deiner" }, { "creatorType": "author", "firstName": "Vasco", "lastName": "Elbrecht" }, { "creatorType": "author", "firstName": "Álvaro", "lastName": "Fueyo" }, { "creatorType": "author", "firstName": "Tim", "lastName": "Goodall" }, { "creatorType": "author", "firstName": "Javier", "lastName": "Granero‐Castro" }, { "creatorType": "author", "firstName": "Cristina", "lastName": "Hartmann‐Fatu" }, { "creatorType": "author", "firstName": "Eleanor", "lastName": "Jones" }, { "creatorType": "author", "firstName": "Elvira", "lastName": "Mächler" }, { "creatorType": "author", "firstName": "Filipa M. S.", "lastName": "Martins" }, { "creatorType": "author", "firstName": "Sam", "lastName": "McGreig" }, { "creatorType": "author", "firstName": "Lyndall", "lastName": "Pereira‐da‐Conceicoa" }, { "creatorType": "author", "firstName": "James L.", "lastName": "Pretty" }, { "creatorType": "author", "firstName": "Daniel S.", "lastName": "Read" }, { "creatorType": "author", "firstName": "Ben", "lastName": "Price" }, { "creatorType": "author", "firstName": "Florian", "lastName": "Leese" }, { "creatorType": "author", "firstName": "Rosetta C.", "lastName": "Blackman" } ], "abstractNote": "Abstract \n \n \n \n Advancements have been made in the use of DNA‐based methods for the detection of single species. However, the routine application of DNA‐based methods to monitor whole communities using a metabarcoding approach and derive ecosystem status continues to be limited. \n \n \n We undertook a structured experiment to assess factors influencing the precision and accuracy of molecular methods for freshwater macroinvertebrate community assessment. Macroinvertebrates were sorted, identified and counted from kick‐net samples using standard morphometric protocols, then reconstituted. These bulk specimen samples were then homogenised. From each bulk sample, aliquots of the homogenate were distributed among seven laboratories across Europe for DNA extraction, PCR amplification, library preparation and sequencing. Additionally, each laboratory was provided with a DNA extract from the bulk homogenate to allow for amplification through to sequencing, in order to assess the influence of DNA extraction. Hierarchical nested analyses were used to identify sources of uncertainty in the data and explore factors influencing the returned community and metrics of ecosystem quality. Furthermore, comparison with morphological analysis enabled us to benchmark molecular to traditional processing approaches. \n \n \n Metrics derived by morphological and DNA‐based methods differed substantially. The strongest methodological influences on variance in metrics derived from DNA‐based methods were attributed to sequencing (read count per sample) and laboratory (strong variation within samples). Furthermore, the performance of the DNA‐based method varied among samples, most likely due to differences in the composition of the invertebrate community. Logistic regression indicated that detectability did not vary among taxa, but that taxa occurring in low abundance (less than five individuals in our samples) were more likely to be missed via metabarcoding. \n \n \n Our findings highlight sources of uncertainty influencing DNA‐based methods and, particularly, the variability of results generated from individual laboratories. Our findings underline the need for clear guidelines, standardisation and quality assurance schemes.", "publicationTitle": "Methods in Ecology and Evolution", "publisher": "", "place": "", "date": "08/2025", "volume": "16", "issue": "8", "section": "", "partNumber": "", "partTitle": "", "pages": "1658-1673", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "Methods Ecol Evol", "DOI": "10.1111/2041-210X.70093", "citationKey": "", "url": "https://besjournals.onlinelibrary.wiley.com/doi/10.1111/2041-210X.70093", "accessDate": "2025-11-03T14:05:49Z", "PMID": "", "PMCID": "", "ISSN": "2041-210X, 2041-210X", "archive": "", "archiveLocation": "", "shortTitle": "Sources of uncertainty in e14023 MER-23-0497.R3

", "tags": [], "relations": {}, "dateAdded": "2025-11-03T13:57:20Z", "dateModified": "2025-11-03T13:57:20Z" } }, { "key": "7GFVEDH4", "version": 986, "library": { "type": "group", "id": 369745, "name": "ABOL Literatur", "links": { "alternate": { "href": "https://www.zotero.org/groups/abol_literatur", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/369745/items/7GFVEDH4", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/abol_literatur/items/7GFVEDH4", "type": "text/html" } }, "meta": { "createdByUser": { "id": 2033903, "username": "abol", "name": "ABOL", "links": { "alternate": { "href": "https://www.zotero.org/abol", "type": "text/html" } } }, "creatorSummary": "Buchner et al.", "parsedDate": "2025", "numChildren": 3 }, "data": { "key": "7GFVEDH4", "version": 986, "itemType": "journalArticle", "title": "Upscaling biodiversity monitoring: Metabarcoding estimates 31,846 insect species from Malaise traps across Germany", "creators": [ { "creatorType": "author", "firstName": "Dominik", "lastName": "Buchner" }, { "creatorType": "author", "firstName": "James S.", "lastName": "Sinclair" }, { "creatorType": "author", "firstName": "Manfred", "lastName": "Ayasse" }, { "creatorType": "author", "firstName": "Arne J.", "lastName": "Beermann" }, { "creatorType": "author", "firstName": "Jörn", "lastName": "Buse" }, { "creatorType": "author", "firstName": "Frank", "lastName": "Dziock" }, { "creatorType": "author", "firstName": "Julian", "lastName": "Enss" }, { "creatorType": "author", "firstName": "Mark", "lastName": "Frenzel" }, { "creatorType": "author", "firstName": "Thomas", "lastName": "Hörren" }, { "creatorType": "author", "firstName": "Yuanheng", "lastName": "Li" }, { "creatorType": "author", "firstName": "Michael T.", "lastName": "Monaghan" }, { "creatorType": "author", "firstName": "Carsten", "lastName": "Morkel" }, { "creatorType": "author", "firstName": "Jörg", "lastName": "Müller" }, { "creatorType": "author", "firstName": "Steffen U.", "lastName": "Pauls" }, { "creatorType": "author", "firstName": "Ronny", "lastName": "Richter" }, { "creatorType": "author", "firstName": "Tobias", "lastName": "Scharnweber" }, { "creatorType": "author", "firstName": "Martin", "lastName": "Sorg" }, { "creatorType": "author", "firstName": "Stefan", "lastName": "Stoll" }, { "creatorType": "author", "firstName": "Sönke", "lastName": "Twietmeyer" }, { "creatorType": "author", "firstName": "Wolfgang W.", "lastName": "Weisser" }, { "creatorType": "author", "firstName": "Benedikt", "lastName": "Wiggering" }, { "creatorType": "author", "firstName": "Martin", "lastName": "Wilmking" }, { "creatorType": "author", "firstName": "Gerhard", "lastName": "Zotz" }, { "creatorType": "author", "firstName": "Mark O.", "lastName": "Gessner" }, { "creatorType": "author", "firstName": "Peter", "lastName": "Haase" }, { "creatorType": "author", "firstName": "Florian", "lastName": "Leese" } ], "abstractNote": "Mitigating ongoing losses of insects and their key functions (e.g. pollination) requires tracking large-scale and long-term community changes. However, doing so has been hindered by the high diversity of insect species that requires prohibitively high investments of time, funding and taxonomic expertise when addressed with conventional tools. Here, we show that these concerns can be addressed through a comprehensive, scalable and cost-efficient DNA metabarcoding workflow. We use 1815 samples from 75 Malaise traps across Germany from 2019 and 2020 to demonstrate how metabarcoding can be incorporated into large-scale insect monitoring networks for less than 50 € per sample, including supplies, labour and maintenance. We validated the detected species using two publicly available databases (GBOL and GBIF) and the judgement of taxonomic experts. With an average of 1.4 M sequence reads per sample we uncovered 10,803 validated insect species, of which 83.9% were represented by a single Operational Taxonomic Unit (OTU). We estimated another 21,043 plausible species, which we argue either lack a reference barcode or are undescribed. The total of 31,846 species is similar to the number of insect species known for Germany ( 35,500). Because Malaise traps capture only a subset of insects, our approach identified many species likely unknown from Germany or new to science. Our reproducible workflow ( 80% OTU-similarity among years) provides a blueprint for large-scale biodiversity monitoring of insects and other biodiversity components in near real time.", "publicationTitle": "Molecular Ecology Resources", "publisher": "", "place": "", "date": "2025", "volume": "25", "issue": "1", "section": "", "partNumber": "", "partTitle": "", "pages": "e14023", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "", "DOI": "10.1111/1755-0998.14023", "citationKey": "", "url": "https://onlinelibrary.wiley.com/doi/abs/10.1111/1755-0998.14023", "accessDate": "2025-11-03T13:56:39Z", "PMID": "", "PMCID": "", "ISSN": "1755-0998", "archive": "", "archiveLocation": "", "shortTitle": "Upscaling biodiversity monitoring", "language": "en", "libraryCatalog": "Wiley Online Library", "callNumber": "", "rights": "© 2024 The Author(s). Molecular Ecology Resources published by John Wiley & Sons Ltd.", "extra": "", "tags": [ { "tag": "DNA metabarcoding", "type": 1 }, { "tag": "Malaise trap", "type": 1 }, { "tag": "biodiversity monitoring", "type": 1 }, { "tag": "insect diversity", "type": 1 } ], "collections": [ "PZDJ5JA3" ], "relations": {}, "dateAdded": "2025-11-03T13:57:19Z", "dateModified": "2025-11-03T13:57:19Z" } }, { "key": "3PVU7UTZ", "version": 984, "library": { "type": "group", "id": 369745, "name": "ABOL Literatur", "links": { "alternate": { "href": "https://www.zotero.org/groups/abol_literatur", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/369745/items/3PVU7UTZ", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/abol_literatur/items/3PVU7UTZ", "type": "text/html" }, "up": { "href": "https://api.zotero.org/groups/369745/items/7XE5X6JJ", "type": "application/json" } }, "meta": { "createdByUser": { "id": 2033903, "username": "abol", "name": "ABOL", "links": { "alternate": { "href": "https://www.zotero.org/abol", "type": "text/html" } } }, "lastModifiedByUser": { "id": 1061585, "username": "nhm_vienna_crl", "name": "", "links": { "alternate": { "href": "https://www.zotero.org/nhm_vienna_crl", "type": "text/html" } } }, "numChildren": 0 }, "data": { "key": "3PVU7UTZ", "version": 984, "parentItem": "7XE5X6JJ", "itemType": "attachment", "linkMode": "imported_url", "title": "Full Text PDF", "accessDate": "2025-05-07T15:10:31Z", "url": "https://www.mdpi.com/1424-2818/14/3/213/pdf?version=1647317550", "note": "", "contentType": "application/pdf", "charset": "", "filename": "Gostel and Kress - 2022 - The Expanding Role of DNA Barcodes Indispensable.pdf", "md5": "237c67ad65c7e7c02b0f06628fccc2ec", "mtime": 1746630632968, "tags": [], "relations": {}, "dateAdded": "2025-05-07T15:10:51Z", "dateModified": "2025-05-07T15:10:51Z" } }, { "key": "7XE5X6JJ", "version": 981, "library": { "type": "group", "id": 369745, "name": "ABOL Literatur", "links": { "alternate": { "href": "https://www.zotero.org/groups/abol_literatur", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/369745/items/7XE5X6JJ", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/abol_literatur/items/7XE5X6JJ", "type": "text/html" } }, "meta": { "createdByUser": { "id": 2033903, "username": "abol", "name": "ABOL", "links": { "alternate": { "href": "https://www.zotero.org/abol", "type": "text/html" } } }, "creatorSummary": "Gostel and Kress", "parsedDate": "2022-03", "numChildren": 1 }, "data": { "key": "7XE5X6JJ", "version": 981, "itemType": "journalArticle", "title": "The Expanding Role of DNA Barcodes: Indispensable Tools for Ecology, Evolution, and Conservation", "creators": [ { "creatorType": "author", "firstName": "Morgan R.", "lastName": "Gostel" }, { "creatorType": "author", "firstName": "W. John", "lastName": "Kress" } ], "abstractNote": "DNA barcoding has transformed the fields of ecology, evolution, and conservation by providing a rapid and effective tool for species identification. The growth of DNA barcodes as a resource for biologists has followed advances in computational and sequencing technology that have enabled high-throughput barcoding applications. The global DNA barcode database is expanding to represent the diversity of species on Earth thanks to efforts by international consortia and expanding biological collections. Today, DNA barcoding is instrumental in advancing our understanding of how species evolve, how they interact, and how we can slow down their extirpation and extinction. 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Other standardized regions meeting barcoding criteria then are also evolving as DNA barcodes for fast, reliable and inexpensive assessment of species composition across all forms of life, including animals, plants, fungi, bacteria and other microorganisms. Consequently, global DNA barcoding campaigns have resulted in the formation of many online workbenches and databases, such as BOLD system, as barcode references, and facilitated the development of mini-barcodes and metabarcoding strategies as important extensions of barcode techniques. Here we intend to give an overview of the characteristics and features of these barcode markers and major reference libraries existing for barcoding the planet’s life, as well as to address the limitations and opportunities of DNA barcodes to an increasingly broader community of science and society.", "publicationTitle": "Conservation Genetics Resources", "publisher": "", "place": "", "date": "2022-12-01", "volume": "14", "issue": "4", "section": "", "partNumber": "", "partTitle": "", "pages": "351-365", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "Conservation Genet Resour", "DOI": "10.1007/s12686-022-01291-2", "citationKey": "", "url": "https://doi.org/10.1007/s12686-022-01291-2", "accessDate": "2025-05-07T15:09:44Z", "PMID": "", "PMCID": "", "ISSN": "1877-7260", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "en", "libraryCatalog": "Springer Link", "callNumber": "", "rights": "", "extra": "", "tags": [ { "tag": "COI", "type": 1 }, { "tag": "DNA barcode", "type": 1 }, { "tag": "DNA metabarcoding", "type": 1 }, { "tag": "Mini-barcode", "type": 1 }, { "tag": "Reference libraries", "type": 1 } ], "collections": [ "WMIQRWZI" ], "relations": {}, "dateAdded": "2025-05-07T15:10:01Z", "dateModified": "2025-05-07T15:10:01Z" } }, { "key": "WCZFFCEB", "version": 977, "library": { "type": "group", "id": 369745, "name": "ABOL Literatur", "links": { "alternate": { "href": "https://www.zotero.org/groups/abol_literatur", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/369745/items/WCZFFCEB", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/abol_literatur/items/WCZFFCEB", "type": "text/html" }, "up": { "href": "https://api.zotero.org/groups/369745/items/J65ZPCAK", "type": "application/json" } }, "meta": { "createdByUser": { "id": 2033903, "username": "abol", "name": "ABOL", "links": { "alternate": { "href": "https://www.zotero.org/abol", "type": "text/html" } } } }, "data": { "key": "WCZFFCEB", "version": 977, "parentItem": "J65ZPCAK", "itemType": "attachment", "linkMode": "linked_url", "title": "PubMed entry", "accessDate": "2025-05-07T15:08:37Z", "url": "http://www.ncbi.nlm.nih.gov/pubmed/36907721", "note": "", "contentType": "", "charset": "", "tags": [], "relations": {}, "dateAdded": "2025-05-07T15:08:52Z", "dateModified": "2025-05-07T15:08:52Z" } }, { "key": "XVHDQPQM", "version": 977, "library": { "type": "group", "id": 369745, "name": "ABOL Literatur", "links": { "alternate": { "href": "https://www.zotero.org/groups/abol_literatur", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/369745/items/XVHDQPQM", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/abol_literatur/items/XVHDQPQM", "type": "text/html" }, "up": { "href": "https://api.zotero.org/groups/369745/items/J65ZPCAK", "type": "application/json" } }, "meta": { "createdByUser": { "id": 2033903, "username": "abol", "name": "ABOL", "links": { "alternate": { "href": "https://www.zotero.org/abol", "type": "text/html" } } } }, "data": { "key": "XVHDQPQM", "version": 977, "parentItem": "J65ZPCAK", "itemType": "attachment", "linkMode": "imported_url", "title": "Full Text PDF", "accessDate": "2025-05-07T15:08:37Z", "url": "https://www.cell.com/action/showPdf?pii=S0168952523000380", "note": "", "contentType": "", "charset": "", "filename": "", "md5": null, "mtime": null, "tags": [], "relations": {}, "dateAdded": "2025-05-07T15:08:52Z", "dateModified": "2025-05-07T15:08:52Z" } }, { "key": "J65ZPCAK", "version": 1007, "library": { "type": "group", "id": 369745, "name": "ABOL Literatur", "links": { "alternate": { "href": "https://www.zotero.org/groups/abol_literatur", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/369745/items/J65ZPCAK", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/abol_literatur/items/J65ZPCAK", "type": "text/html" } }, "meta": { "createdByUser": { "id": 2033903, "username": "abol", "name": "ABOL", "links": { "alternate": { "href": "https://www.zotero.org/abol", "type": "text/html" } } }, "lastModifiedByUser": { "id": 1061585, "username": "nhm_vienna_crl", "name": "", "links": { "alternate": { "href": "https://www.zotero.org/nhm_vienna_crl", "type": "text/html" } } }, "creatorSummary": "Chua et al.", "parsedDate": "2023-07-01", "numChildren": 2 }, "data": { "key": "J65ZPCAK", "version": 1007, "itemType": "journalArticle", "title": "Future of DNA-based insect monitoring", "creators": [ { "creatorType": "author", "firstName": "Physilia Y. 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2022 - Pragmatic Applications and Universality of DNA Bar.pdf", "md5": "1432f21ed9fbd78f54850f0c4ae4b44d", "mtime": 1746630439963, "tags": [], "relations": {}, "dateAdded": "2025-05-07T15:08:01Z", "dateModified": "2025-05-07T15:08:01Z" } }, { "key": "2UKWS9MR", "version": 972, "library": { "type": "group", "id": 369745, "name": "ABOL Literatur", "links": { "alternate": { "href": "https://www.zotero.org/groups/abol_literatur", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/369745/items/2UKWS9MR", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/abol_literatur/items/2UKWS9MR", "type": "text/html" } }, "meta": { "createdByUser": { "id": 2033903, "username": "abol", "name": "ABOL", "links": { "alternate": { "href": "https://www.zotero.org/abol", "type": "text/html" } } }, "creatorSummary": "Ahmed et al.", "parsedDate": "2022", "numChildren": 2 }, "data": { "key": "2UKWS9MR", "version": 972, "itemType": "journalArticle", "title": "Pragmatic Applications and Universality of DNA Barcoding for Substantial Organisms at Species Level: A Review to Explore a Way Forward", "creators": [ { "creatorType": "author", "firstName": "Sarfraz", "lastName": "Ahmed" }, { "creatorType": "author", "firstName": "Muhammad", "lastName": "Ibrahim" }, { "creatorType": "author", "firstName": "Chanin", "lastName": "Nantasenamat" }, { "creatorType": "author", "firstName": "Muhammad Farrukh", "lastName": "Nisar" }, { "creatorType": "author", "firstName": "Aijaz Ahmad", "lastName": "Malik" }, { "creatorType": "author", "firstName": "Rashem", "lastName": "Waheed" }, { "creatorType": "author", "firstName": "Muhammad Z.", "lastName": "Ahmed" }, { "creatorType": "author", "firstName": "Suvash Chandra", "lastName": "Ojha" }, { "creatorType": "author", "firstName": "Mohammad Khursheed", "lastName": "Alam" } ], "abstractNote": "DNA barcodes are regarded as hereditary succession codes that serve as a recognition marker to address several queries relating to the identification, classification, community ecology, and evolution of certain functional traits in organisms. The mitochondrial cytochrome c oxidase 1 (CO1) gene as a DNA barcode is highly efficient for discriminating vertebrate and invertebrate animal species. Similarly, different specific markers are used for other organisms, including ribulose bisphosphate carboxylase (rbcL), maturase kinase (matK), transfer RNA-H and photosystem II D1-ApbsArabidopsis thaliana (trnH-psbA), and internal transcribed spacer (ITS) for plant species; 16S ribosomal RNA (16S rRNA), elongation factor Tu gene (Tuf gene), and chaperonin for bacterial strains; and nuclear ITS for fungal strains. Nevertheless, the taxon coverage of reference sequences is far from complete for genus or species-level identification. Applying the next-generation sequencing approach to the parallel acquisition of DNA barcode sequences could greatly expand the potential for library preparation or accurate identification in biodiversity research. Overall, this review articulates on the DNA barcoding technology as applied to different organisms, its universality, applicability, and innovative approach to handling DNA-based species identification.", "publicationTitle": "BioMed Research International", "publisher": "", "place": "", "date": "2022", "volume": "2022", "issue": "1", "section": "", "partNumber": "", "partTitle": "", "pages": "1846485", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "", "DOI": "10.1155/2022/1846485", "citationKey": "", "url": "https://onlinelibrary.wiley.com/doi/abs/10.1155/2022/1846485", "accessDate": "2025-05-07T15:07:08Z", "PMID": "", "PMCID": "", "ISSN": "2314-6141", "archive": "", "archiveLocation": "", "shortTitle": "Pragmatic Applications and Universality of DNA Barcoding for Substantial Organisms at Species Level", "language": "en", "libraryCatalog": "Wiley Online Library", "callNumber": "", "rights": "Copyright © 2022 Sarfraz Ahmed et al.", "extra": "", "tags": [], "collections": [ "WMIQRWZI" ], "relations": {}, "dateAdded": "2025-05-07T15:07:59Z", "dateModified": "2025-05-07T15:07:59Z" } } ]