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2011 - A potential role for α-actinin in inside-out αIIbβ.pdf", "md5": null, "mtime": null, "tags": [], "relations": {}, "dateAdded": "2016-04-19T02:28:26Z", "dateModified": "2016-04-19T02:28:26Z" } }, { "key": "HV775D2G", "version": 263, "library": { "type": "group", "id": 363678, "name": "Team BLOOD", "links": { "alternate": { "href": "https://www.zotero.org/groups/team_blood", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/363678/items/HV775D2G", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/team_blood/items/HV775D2G", "type": "text/html" } }, "meta": { "createdByUser": { "id": 2346930, "username": "awalls", "name": "", "links": { "alternate": { "href": "https://www.zotero.org/awalls", "type": "text/html" } } }, "creatorSummary": "Chen et al.", "parsedDate": "2015-11-15", "numChildren": 2 }, "data": { "key": "HV775D2G", "version": 263, "itemType": "journalArticle", "title": "Megakaryocytic differentiation of mouse embryonic stem cells via coculture with immortalized OP9 stromal cells", "creators": [ { "creatorType": "author", "firstName": "Po-Kong", "lastName": "Chen" }, { "creatorType": "author", "firstName": "Guan-Ling", "lastName": "Lin" }, { "creatorType": "author", "firstName": "Hsin-Hou", "lastName": "Chang" }, { "creatorType": "author", "firstName": "Der-Shan", "lastName": "Sun" } ], "abstractNote": "Established from the calvaria of newborn macrophage colony-stimulating factor (M-CSF)-deficient mice, OP9 is a stromal cell line that used as a feeder layer to support the in vitro differentiation of pluripotent stem cells into various hematopoietic lineage cells, including granulocytes, erythrocytes, lymphocytes, and megakaryocytes. However, as a primary culture cell line, OP9 can be used as stromal cells for only 1 month. Therefore, to obtain functional OP9 cells, numerous M-CSF-deficient newborn mice must be sacrificed. These limitations in some ways restrict the application of OP9 cells in longterm and largescale experiments. In this study, we used human papillomavirus 16 E6 and E7 genes to generate immortalized OP9 stromal cells, designated I-OP9 cells, and then tested their ability to support the megakaryocytic differentiation of pluripotent stem cells in vitro. I-OP9 cells have similar morphology and properties as do parental OP9 cells, and, as expected, have an extended lifespan and can support megakaryocytic differentiation. Our data suggest that the method used in this study, including establishing I-OP9 cells, enables the possibility to enlarge and lengthen the scale of the experiment and, more critically, provides a humanistic approach for preparing stromal cells that support the hematopoietic differentiation of pluripotent stem cells in vitro.", "publicationTitle": "Experimental Cell Research", "publisher": "", "place": "", "date": "November 15, 2015", "volume": "339", "issue": "1", "section": "", "partNumber": "", "partTitle": "", "pages": "44-50", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "Experimental Cell Research", "DOI": "10.1016/j.yexcr.2015.10.002", "citationKey": "", "url": "http://www.sciencedirect.com/science/article/pii/S0014482715301087", "accessDate": "2016-04-19T02:12:04Z", "PMID": "", "PMCID": "", "ISSN": "0014-4827", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "", "libraryCatalog": "ScienceDirect", "callNumber": "", "rights": "", "extra": "", "tags": [ { "tag": "Immortalization", "type": 1 }, { "tag": "Megakaryocytic differentiation", "type": 1 }, { "tag": "OP9 stromal cells", "type": 1 } ], "collections": [ "VSSNN4ZS" ], "relations": {}, "dateAdded": "2016-04-19T02:28:26Z", "dateModified": "2016-04-19T02:28:26Z" } }, { "key": "BDQK627W", "version": 263, "library": { "type": "group", "id": 363678, "name": "Team BLOOD", "links": { "alternate": { "href": "https://www.zotero.org/groups/team_blood", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/363678/items/BDQK627W", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/team_blood/items/BDQK627W", "type": "text/html" } }, "meta": { "createdByUser": { "id": 2346930, "username": "awalls", "name": "", "links": { "alternate": { "href": "https://www.zotero.org/awalls", "type": "text/html" } } }, "creatorSummary": "Figueiredo et al.", "parsedDate": "2015-08-21", "numChildren": 2 }, "data": { "key": "BDQK627W", "version": 263, "itemType": "journalArticle", "title": "OP9 Stromal Cells Proteins Involved in Hematoendothelial Differentiation from Human Embryonic Stem Cells", "creators": [ { "creatorType": "author", "firstName": "Lilian M.", "lastName": "Figueiredo" }, { "creatorType": "author", "firstName": "Everton B.O.", "lastName": "Costa" }, { "creatorType": "author", "firstName": "Maristela D.", "lastName": "Orellana" }, { "creatorType": "author", "firstName": "Virginia", "lastName": "Picanço-Castro" }, { "creatorType": "author", "firstName": "Dimas T.", "lastName": "Covas" } ], "abstractNote": "Hematopoietic cells (HCs) and endothelial cells (ECs) can be produced in vitro from human embryonic stem cells (hESCs), but the differentiation systems used are still inefficient. To overcome this obstacle, it is necessary to understand the differentiation process. One of the methods used to obtain HCs and ECs from hESCs is their co-culture with stromal cells. The soluble factors secreted by these cells and cell–cell contact have a great impact on the differentiation process. Here, we performed comparative proteomic analyses of proteins obtained from the total extract of OP9 stromal cells and secreted by these cells before and during in vitro generation of HCs and ECs (hematoendothelial) from hESCs. We identified a total of 83 secreted and 759 intracellular proteins during differentiation. Twenty-five secreted and 181 proteins from the total extract were more abundant. Some secreted proteins are involved in cell–matrix interactions and HC and/or EC development. Moreover, 13 proteins of the total extract from OP9 cells that were exclusive/or more abundant during differentiation are involved in the Nrf2/Nfe2l2 gene pathway, that is, they are described to have a key role in oxidative stress and in hematopoietic development and maturation. Our proteomic profiles provide valuable insight about the proteins involved in in vitro hematoendothelial cell generation and in the future they might be used to optimize the differentiation process and produce both cell types in vitro.", "publicationTitle": "Cellular Reprogramming", "publisher": "", "place": "", "date": "August 21, 2015", "volume": "17", "issue": "5", "section": "", "partNumber": "", "partTitle": "", "pages": "338-346", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "Cellular Reprogramming", "DOI": "10.1089/cell.2015.0014", "citationKey": "", "url": "http://online.liebertpub.com/doi/abs/10.1089/cell.2015.0014", "accessDate": "2016-04-19T02:19:39Z", "PMID": "", "PMCID": "", "ISSN": "2152-4971", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "", "libraryCatalog": "online.liebertpub.com (Atypon)", "callNumber": "", "rights": "", "extra": "", "tags": [], "collections": [ "VSSNN4ZS" ], "relations": {}, "dateAdded": "2016-04-19T02:28:26Z", "dateModified": "2016-04-19T02:28:26Z" } }, { "key": "IWFJ6TS2", "version": 263, "library": { "type": "group", "id": 363678, "name": "Team BLOOD", "links": { "alternate": { "href": "https://www.zotero.org/groups/team_blood", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/363678/items/IWFJ6TS2", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/team_blood/items/IWFJ6TS2", "type": "text/html" } }, "meta": { "createdByUser": { "id": 2346930, "username": "awalls", "name": "", "links": { "alternate": { "href": "https://www.zotero.org/awalls", "type": "text/html" } } }, "creatorSummary": "Tadokoro et al.", "parsedDate": "2011-01-06", "numChildren": 3 }, "data": { "key": "IWFJ6TS2", "version": 263, "itemType": "journalArticle", "title": "A potential role for α-actinin in inside-out αIIbβ3 signaling", "creators": [ { "creatorType": "author", "firstName": "Seiji", "lastName": "Tadokoro" }, { "creatorType": "author", "firstName": "Tsuyoshi", "lastName": "Nakazawa" }, { "creatorType": "author", "firstName": "Tsuyoshi", "lastName": "Kamae" }, { "creatorType": "author", "firstName": "Kazunobu", "lastName": "Kiyomizu" }, { "creatorType": "author", "firstName": "Hirokazu", "lastName": "Kashiwagi" }, { "creatorType": "author", "firstName": "Shigenori", "lastName": "Honda" }, { "creatorType": "author", "firstName": "Yuzuru", "lastName": "Kanakura" }, { "creatorType": "author", "firstName": "Yoshiaki", "lastName": "Tomiyama" } ], "abstractNote": "Many different biochemical signaling pathways regulate integrin activation through the integrin cytoplasmic tail. Here, we describe a new role for α-actinin in inside-out integrin activation. In resting human platelets, α-actinin was associated with αIIbβ3, whereas inside-out signaling (αIIbβ3 activation signals) from protease-activated receptors (PARs) dephosphorylated and dissociated α-actinin from αIIbβ3. We evaluated the time-dependent changes of the αIIbβ3 activation state by measuring PAC-1 binding velocity. The initial velocity analysis clearly showed that PAR1-activating peptide stimulation induced only transient αIIbβ3 activation, whereas PAR4-activating peptide induced long-lasting αIIbβ3 activation. When αIIbβ3 activation signaling dwindled, α-actinin became rephosphorylated and reassociated with αIIbβ3. Compared with control platelets, the dissociation of α-actinin from αIIbβ3 was only transient in PAR4-stimulated P2Y12-deficient platelets in which the sustained αIIbβ3 activation was markedly impaired. Overexpression of wild-type α-actinin, but not the mutant Y12F α-actinin, increased its binding to αIIbβ3 and inhibited PAR1-induced initial αIIbβ3 activation in the human megakaryoblastic cell line, CMK. In contrast, knockdown of α-actinin augmented PAR-induced αIIbβ3 activation in CMK. These observations suggest that α-actinin might play a potential role in setting integrins to a default low-affinity ligand-binding state in resting platelets and regulating αIIbβ3 activation by inside-out signaling.", "publicationTitle": "Blood", "publisher": "", "place": "", "date": "2011/01/06", "volume": "117", "issue": "1", "section": "", "partNumber": "", "partTitle": "", "pages": "250-258", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "", "DOI": "10.1182/blood-2009-10-246751", "citationKey": "", "url": "http://www.bloodjournal.org/content/117/1/250", "accessDate": "2016-04-19T02:23:35Z", "PMID": "", "PMCID": "", "ISSN": "0006-4971, 1528-0020", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "en", "libraryCatalog": "www.bloodjournal.org", "callNumber": "", "rights": "© 2011 by The American Society of Hematology", "extra": "PMID: 20940419", "tags": [], "collections": [ "VSSNN4ZS" ], "relations": {}, "dateAdded": "2016-04-19T02:28:26Z", "dateModified": "2016-04-19T02:28:26Z" } }, { "key": "XQPRTJAM", "version": 265, "library": { "type": "group", "id": 363678, "name": "Team BLOOD", "links": { "alternate": { "href": "https://www.zotero.org/groups/team_blood", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/363678/items/XQPRTJAM", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/team_blood/items/XQPRTJAM", "type": "text/html" }, "up": { "href": "https://api.zotero.org/groups/363678/items/9IEMF52D", "type": "application/json" } }, "meta": { "createdByUser": { "id": 2346930, "username": "awalls", "name": "", "links": { "alternate": { "href": "https://www.zotero.org/awalls", "type": "text/html" } } }, "lastModifiedByUser": { "id": 2366100, "username": "amilsahai", "name": "", "links": { "alternate": { "href": "https://www.zotero.org/amilsahai", "type": "text/html" } } } }, "data": { "key": "XQPRTJAM", "version": 265, "parentItem": "9IEMF52D", "itemType": "attachment", "linkMode": "linked_url", "title": "PubMed entry", "accessDate": "2016-04-07T12:25:10Z", "url": "http://www.ncbi.nlm.nih.gov/pubmed/1375113", "note": "", "contentType": "text/html", "charset": "", "tags": [], "relations": {}, "dateAdded": "2016-04-07T12:26:05Z", "dateModified": "2016-04-07T12:26:05Z" } }, { "key": "HBQHE3ME", "version": 265, "library": { "type": "group", "id": 363678, "name": "Team BLOOD", "links": { "alternate": { "href": "https://www.zotero.org/groups/team_blood", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/363678/items/HBQHE3ME", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/team_blood/items/HBQHE3ME", "type": "text/html" }, "up": { "href": "https://api.zotero.org/groups/363678/items/9IEMF52D", "type": "application/json" } }, "meta": { "createdByUser": { "id": 2346930, "username": "awalls", "name": "", "links": { "alternate": { "href": "https://www.zotero.org/awalls", "type": "text/html" } } }, "lastModifiedByUser": { "id": 2366100, "username": "amilsahai", "name": "", "links": { "alternate": { "href": "https://www.zotero.org/amilsahai", "type": "text/html" } } } }, "data": { "key": "HBQHE3ME", "version": 265, "parentItem": "9IEMF52D", "itemType": "attachment", "linkMode": "imported_url", "title": "Snapshot", "accessDate": "2016-04-07T12:25:16Z", "url": "http://www.bloodjournal.org/content/79/11/2821.short?sso-checked=true", "note": "", "contentType": "text/html", "charset": "utf-8", "filename": "2821.html", "md5": "dc701636f2244dcd5bf989ad9d944c72", "mtime": 1460031917179, "tags": [], "relations": {}, "dateAdded": "2016-04-07T12:26:05Z", "dateModified": "2016-04-07T12:26:05Z" } }, { "key": "VCB29I5V", "version": 265, "library": { "type": "group", "id": 363678, "name": "Team BLOOD", "links": { "alternate": { "href": "https://www.zotero.org/groups/team_blood", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/363678/items/VCB29I5V", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/team_blood/items/VCB29I5V", "type": "text/html" }, "up": { "href": "https://api.zotero.org/groups/363678/items/9IEMF52D", "type": "application/json" } }, "meta": { "createdByUser": { "id": 2346930, "username": "awalls", "name": "", "links": { "alternate": { "href": "https://www.zotero.org/awalls", "type": "text/html" } } }, "lastModifiedByUser": { "id": 2366100, "username": "amilsahai", "name": "", "links": { "alternate": { "href": "https://www.zotero.org/amilsahai", "type": "text/html" } } }, "numChildren": 0 }, "data": { "key": "VCB29I5V", "version": 265, "parentItem": "9IEMF52D", "itemType": "attachment", "linkMode": "imported_url", "title": "Full Text PDF", "accessDate": "2016-04-07T12:25:10Z", "url": "http://www.bloodjournal.org/content/bloodjournal/79/11/2821.full.pdf", "note": "", "contentType": "application/pdf", "charset": "", "filename": "Verfaillie - 1992 - Direct contact between human primitive hematopoiet.pdf", "md5": "ca081799e61612461f489ac01ee8704f", "mtime": 1460031925128, "tags": [], "relations": {}, "dateAdded": "2016-04-07T12:26:05Z", "dateModified": "2016-04-07T12:26:05Z" } }, { "key": "9IEMF52D", "version": 261, "library": { "type": "group", "id": 363678, "name": "Team BLOOD", "links": { "alternate": { "href": "https://www.zotero.org/groups/team_blood", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/363678/items/9IEMF52D", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/team_blood/items/9IEMF52D", "type": "text/html" } }, "meta": { "createdByUser": { "id": 2346930, "username": "awalls", "name": "", "links": { "alternate": { "href": "https://www.zotero.org/awalls", "type": "text/html" } } }, "creatorSummary": "Verfaillie", "parsedDate": "1992-06-01", "numChildren": 3 }, "data": { "key": "9IEMF52D", "version": 261, "itemType": "journalArticle", "title": "Direct contact between human primitive hematopoietic progenitors and bone marrow stroma is not required for long-term in vitro hematopoiesis", "creators": [ { "creatorType": "author", "firstName": "C. M.", "lastName": "Verfaillie" } ], "abstractNote": "Long-term bone marrow cultures support both differentiation and conservation of primitive human hematopoietic progenitors in the absence of exogenous cytokines. It is believed that hematopoiesis in such cultures requires direct contact between hematopoietic progenitors and stroma. In the present study, we demonstrate that primitive progenitors physically separated from the stromal layer by a 0.45- microns microporous membrane continue to generate differentiated progenitors for at least 8 weeks. Moreover, primitive progenitors are conserved to a greater extent under these conditions, as when cultured in direct contact with the stroma. However, excessive production of granulocyte-macrophage progenitors occurs when primitive progenitors are not allowed to interact directly with the stroma. Thus, direct contact between hematopoietic and stromal cells is not required for either differentiation or conservation of primitive hematopoietic progenitors but is essential for the regulated production of mature blood elements. These findings can now be used to define the role of diffusible factors and cell-cell or cell-extracellular matrix adhesion events in the regulation of conservation, proliferation, and differentiation of primitive human hematopoietic progenitors in vitro.", "publicationTitle": "Blood", "publisher": "", "place": "", "date": "1992/06/01", "volume": "79", "issue": "11", "section": "", "partNumber": "", "partTitle": "", "pages": "2821-2826", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "", "DOI": "", "citationKey": "", "url": "http://www.bloodjournal.org/content/79/11/2821", "accessDate": "2016-04-07T12:25:10Z", "PMID": "", "PMCID": "", "ISSN": "0006-4971, 1528-0020", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "en", "libraryCatalog": "www.bloodjournal.org", "callNumber": "", "rights": "Copyright © 1992 by The American Society of Hematology", "extra": "PMID: 1375113", "tags": [], "collections": [ "VSSNN4ZS" ], "relations": {}, "dateAdded": "2016-04-07T12:26:05Z", "dateModified": "2016-04-07T12:26:05Z" } }, { "key": "CGSNGJ2J", "version": 259, "library": { "type": "group", "id": 363678, "name": "Team BLOOD", "links": { "alternate": { "href": "https://www.zotero.org/groups/team_blood", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/363678/items/CGSNGJ2J", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/team_blood/items/CGSNGJ2J", "type": "text/html" } }, "meta": { "createdByUser": { "id": 2366100, "username": "amilsahai", "name": "", "links": { "alternate": { "href": "https://www.zotero.org/amilsahai", "type": "text/html" } } }, "numChildren": 0 }, "data": { "key": "CGSNGJ2J", "version": 259, "itemType": "journalArticle", "title": "Nicotine induces cell proliferation, invasion and epithelial-mesenchymal transition in a variety of human cancer cell lines", "creators": [], "abstractNote": "", "publicationTitle": "", "publisher": "", 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"username": "amilsahai", "name": "", "links": { "alternate": { "href": "https://www.zotero.org/amilsahai", "type": "text/html" } } }, "numChildren": 0 }, "data": { "key": "7NDSR952", "version": 265, "parentItem": "H9XZRAH2", "itemType": "attachment", "linkMode": "imported_url", "title": "Full Text PDF", "accessDate": "2016-03-01T03:12:03Z", "url": "http://www.bloodjournal.org/content/bloodjournal/102/3/906.full.pdf", "note": "", "contentType": "application/pdf", "charset": "", "filename": "Chadwick et al. - 2003 - Cytokines and BMP-4 promote hematopoietic differen.pdf", "md5": "db57a5f589d1b06bc7875102128955d3", "mtime": 1456801929711, "tags": [], "relations": {}, "dateAdded": "2016-03-01T03:12:17Z", "dateModified": "2016-03-01T03:12:17Z" } }, { "key": "H9XZRAH2", "version": 257, "library": { "type": "group", "id": 363678, "name": "Team BLOOD", "links": { "alternate": { "href": "https://www.zotero.org/groups/team_blood", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/363678/items/H9XZRAH2", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/team_blood/items/H9XZRAH2", "type": "text/html" } }, "meta": { "createdByUser": { "id": 2346930, "username": "awalls", "name": "", "links": { "alternate": { "href": "https://www.zotero.org/awalls", "type": "text/html" } } }, "creatorSummary": "Chadwick et al.", "parsedDate": "2003-08-01", "numChildren": 3 }, "data": { "key": "H9XZRAH2", "version": 257, "itemType": "journalArticle", "title": "Cytokines and BMP-4 promote hematopoietic differentiation of human embryonic stem cells", "creators": [ { "creatorType": "author", "firstName": "Kristin", "lastName": "Chadwick" }, { "creatorType": "author", "firstName": "Lisheng", "lastName": "Wang" }, { "creatorType": "author", "firstName": "Li", "lastName": "Li" }, { "creatorType": "author", "firstName": "Pablo", "lastName": "Menendez" }, { "creatorType": "author", "firstName": "Barbara", "lastName": "Murdoch" }, { "creatorType": "author", "firstName": "Anne", "lastName": "Rouleau" }, { "creatorType": "author", "firstName": "Mickie", "lastName": "Bhatia" } ], "abstractNote": "Human embryonic stem cells (hESCs) randomly differentiate into multiple cell types during embryoid body (EB) development. To date, characterization of specific factors capable of influencing hematopoietic cell fate from hESCs remains elusive. Here, we report that the treatment of hESCs during EB development with a combination of cytokines and bone morphogenetic protein-4 (BMP-4), a ventral mesoderm inducer, strongly promotes hematopoietic differentiation. Hematopoietic progenitors of multiple lineages were generated from EBs and were found to be restricted to the population of progeny expressing cell surface CD45. Addition of BMP-4 had no statistically significant effect on hematopoietic differentiation but enabled significant enhancement in progenitor self-renewal, independent of cytokine treatment. Hematopoietic commitment was characterized as the temporal emergence of single CD45+ cells first detectable after day 10 of culture and was accompanied by expression of hematopoietic transcription factors. Despite the removal of cytokines at day 10, hematopoietic differentiation of hESCs continued, suggesting that cytokines act on hematopoietic precursors as opposed to differentiated hematopoietic cells. Our study establishes the first evidence for the role of cytokines and BMP-4 in promoting hematopoietic differentiation of hESC lines and provides an unprecedented system to study early developmental events that govern the initiation of hematopoiesis in the human.", "publicationTitle": "Blood", "publisher": "", "place": "", "date": "2003/08/01", "volume": "102", "issue": "3", "section": "", "partNumber": "", "partTitle": "", "pages": "906-915", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "", "DOI": "10.1182/blood-2003-03-0832", "citationKey": "", "url": "http://www.bloodjournal.org/content/102/3/906", "accessDate": "2016-03-01T03:12:03Z", "PMID": "", "PMCID": "", "ISSN": "0006-4971, 1528-0020", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "en", "libraryCatalog": "www.bloodjournal.org", "callNumber": "", "rights": "Copyright © 2003 by The American Society of Hematology", "extra": "PMID: 12702499", "tags": [], "collections": [], "relations": {}, "dateAdded": "2016-03-01T03:12:17Z", "dateModified": "2016-03-01T03:12:17Z" } }, { "key": "SF8ZX7NC", "version": 265, "library": { "type": "group", "id": 363678, "name": "Team BLOOD", "links": { "alternate": { "href": "https://www.zotero.org/groups/team_blood", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/363678/items/SF8ZX7NC", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/team_blood/items/SF8ZX7NC", "type": "text/html" }, "up": { "href": "https://api.zotero.org/groups/363678/items/J4D6Z9N9", "type": "application/json" } }, "meta": { "createdByUser": { "id": 2346930, "username": "awalls", "name": "", "links": { "alternate": { "href": "https://www.zotero.org/awalls", "type": "text/html" } } }, "lastModifiedByUser": { "id": 2366100, "username": "amilsahai", "name": "", "links": { "alternate": { "href": "https://www.zotero.org/amilsahai", "type": "text/html" } } }, "numChildren": 0 }, "data": { "key": "SF8ZX7NC", "version": 265, "parentItem": "J4D6Z9N9", "itemType": "attachment", "linkMode": "imported_url", "title": "Full Text PDF", "accessDate": "2016-03-01T02:38:49Z", "url": "http://www.bloodjournal.org/content/bloodjournal/114/9/1764.full.pdf", "note": "", "contentType": "application/pdf", "charset": "", "filename": "Okabe et al. - 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Although proof of cellular origin requires genetic-marker identification in both target cells and established iPSCs, somatic cells other than mature lymphocytes mostly lack such markers. Here we show definitive proof of direct reprogramming of murine hematopoietic cells with no rearranged genes. Using iPSC factor transduction, we successfully derived iPSCs from bone marrow progenitor cells obtained from a mouse whose hematopoiesis was reconstituted from a single congenic hematopoietic stem cell. Established clones were demonstrated to be genetically identical to the transplanted single hematopoietic stem cell, thus proving their cellular origin. These hematopoietic cell–derived iPSCs showed typical characteristics of iPSCs, including the ability to contribute to chimerism in mice. 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