[ { "key": "MUBZA7Q6", "version": 6, "library": { "type": "group", "id": 334868, "name": "Built Enviro Microbiome", "links": { "alternate": { "href": "https://www.zotero.org/groups/built_enviro_microbiome", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/334868/items/MUBZA7Q6", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/built_enviro_microbiome/items/MUBZA7Q6", "type": "text/html" } }, "meta": { "createdByUser": { "id": 36430, "username": "mikefortun", "name": "Mike Fortun", "links": { "alternate": { "href": "https://www.zotero.org/mikefortun", "type": "text/html" } } }, "creatorSummary": "Hoisington et al.", "parsedDate": "2015", "numChildren": 0 }, "data": { "key": "MUBZA7Q6", "version": 6, "itemType": "journalArticle", "title": "The microbiome of the built environment and mental health", "creators": [ { "creatorType": "author", "firstName": "Andrew J.", "lastName": "Hoisington" }, { "creatorType": "author", "firstName": "Lisa A.", "lastName": "Brenner" }, { "creatorType": "author", "firstName": "Kerry A.", "lastName": "Kinney" }, { "creatorType": "author", "firstName": "Teodor T.", "lastName": "Postolache" }, { "creatorType": "author", "firstName": "Christopher A.", "lastName": "Lowry" } ], "abstractNote": "", "publicationTitle": "Microbiome", "publisher": "", "place": "", "date": "12/2015", "volume": "3", "issue": "1", "section": "", "partNumber": "", "partTitle": "", "pages": "", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "", "DOI": "10.1186/s40168-015-0127-0", "citationKey": "", "url": "http://www.microbiomejournal.com/content/3/1/60", "accessDate": "2015-12-31T00:36:02Z", "PMID": "", "PMCID": "", "ISSN": "2049-2618", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "en", "libraryCatalog": "CrossRef", "callNumber": "", "rights": "", "extra": "00000", "tags": [], "collections": [], "relations": {}, "dateAdded": "2015-12-31T00:36:02Z", "dateModified": "2015-12-31T00:36:03Z" } }, { "key": "U6BADKTU", "version": 5, "library": { "type": "group", "id": 334868, "name": "Built Enviro Microbiome", "links": { "alternate": { "href": "https://www.zotero.org/groups/built_enviro_microbiome", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/334868/items/U6BADKTU", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/built_enviro_microbiome/items/U6BADKTU", "type": "text/html" } }, "meta": { "createdByUser": { "id": 36430, "username": "mikefortun", "name": "Mike Fortun", "links": { "alternate": { "href": "https://www.zotero.org/mikefortun", "type": "text/html" } } }, "creatorSummary": "Shong et al.", "parsedDate": "2013-04-19", "numChildren": 2 }, "data": { "key": "U6BADKTU", "version": 5, "itemType": "journalArticle", "title": "Directed Evolution of the Quorum-Sensing Regulator EsaR for Increased Signal Sensitivity", "creators": [ { "creatorType": "author", "firstName": "Jasmine", "lastName": "Shong" }, { "creatorType": "author", "firstName": "Yao-Ming", "lastName": "Huang" }, { "creatorType": "author", "firstName": "Christopher", "lastName": "Bystroff" }, { "creatorType": "author", "firstName": "Cynthia H.", "lastName": "Collins" } ], "abstractNote": "The use of cell?cell communication or ?quorum sensing (QS)? elements from Gram-negative Proteobacteria has enabled synthetic biologists to begin engineering systems composed of multiple interacting organisms. However, additional tools are necessary if we are to progress toward synthetic microbial consortia that exhibit more complex, dynamic behaviors. EsaR from Pantoea stewartii subsp. stewartii is a QS regulator that binds to DNA as an apoprotein and releases the DNA when it binds to its cognate signal molecule, 3-oxohexanoyl-homoserine lactone (3OC6HSL). In the absence of 3OC6HSL, EsaR binds to DNA and can act as either an activator or a repressor of transcription. Gene expression from PesaR, which is repressed by wild-type EsaR, requires 100- to 1000-fold higher concentrations of signal than commonly used QS activators, such as LuxR and LasR. Here we have identified EsaR variants with increased sensitivity to 3OC6HSL using directed evolution and a dual ON/OFF screening strategy. Although we targeted EsaR-dependent derepression of PesaR, our EsaR variants also showed increased 3OC6HSL sensitivity at a second promoter, PesaS, which is activated by EsaR in the absence of 3OC6HSL. Here, the increase in AHL sensitivity led to gene expression being turned off at lower concentrations of 3OC6HSL. Overall, we have increased the signal sensitivity of EsaR more than 70-fold and generated a set of EsaR variants that recognize 3OC6HSL concentrations ranging over 4 orders of magnitude. QS-dependent transcriptional regulators that bind to DNA and are active in the absence of a QS signal represent a new set of tools for engineering cell?cell communication-dependent gene expression.", "publicationTitle": "ACS Chemical Biology", "publisher": "", "place": "", "date": "April 19, 2013", "volume": "8", "issue": "4", "section": "", "partNumber": "", "partTitle": "", "pages": "789-795", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "ACS Chem. Biol.", "DOI": "10.1021/cb3006402", "citationKey": "", "url": "http://dx.doi.org/10.1021/cb3006402", "accessDate": "2015-06-23T09:57:09Z", "PMID": "", "PMCID": "", "ISSN": "1554-8929", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "", "libraryCatalog": "ACS Publications", "callNumber": "", "rights": "", "extra": "00009", "tags": [], "collections": [], "relations": {}, "dateAdded": "2015-06-23T09:57:09Z", "dateModified": "2015-06-23T09:57:10Z" } }, { "key": "SSSDEQSJ", "version": 5, "library": { "type": "group", "id": 334868, "name": "Built Enviro Microbiome", "links": { "alternate": { "href": "https://www.zotero.org/groups/built_enviro_microbiome", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/334868/items/SSSDEQSJ", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/built_enviro_microbiome/items/SSSDEQSJ", "type": "text/html" }, "up": { "href": "https://api.zotero.org/groups/334868/items/U6BADKTU", "type": "application/json" } }, "meta": { "createdByUser": { "id": 36430, "username": "mikefortun", "name": "Mike Fortun", "links": { "alternate": { "href": "https://www.zotero.org/mikefortun", "type": "text/html" } } } }, "data": { "key": "SSSDEQSJ", "version": 5, "parentItem": "U6BADKTU", "itemType": "attachment", "linkMode": "linked_url", "title": "ACS Full Text Snapshot", "accessDate": "2015-06-23T09:57:09Z", "url": "http://pubs.acs.org.libproxy.rpi.edu/doi/full/10.1021/cb3006402", "note": "", "contentType": "text/html", "charset": "", "tags": [], "relations": {}, "dateAdded": "2015-06-23T09:57:09Z", "dateModified": "2015-06-23T09:57:09Z" } }, { "key": "X4RGNGAX", "version": 5, "library": { "type": "group", "id": 334868, "name": "Built Enviro Microbiome", "links": { "alternate": { "href": "https://www.zotero.org/groups/built_enviro_microbiome", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/334868/items/X4RGNGAX", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/built_enviro_microbiome/items/X4RGNGAX", "type": "text/html" }, "up": { "href": "https://api.zotero.org/groups/334868/items/U6BADKTU", "type": "application/json" } }, "meta": { "createdByUser": { "id": 36430, "username": "mikefortun", "name": "Mike Fortun", "links": { "alternate": { "href": "https://www.zotero.org/mikefortun", "type": "text/html" } } } }, "data": { "key": "X4RGNGAX", "version": 5, "parentItem": "U6BADKTU", "itemType": "attachment", "linkMode": "linked_url", "title": "ACS Full Text PDF w/ Links", "accessDate": "2015-06-23T09:57:09Z", "url": "http://pubs.acs.org.libproxy.rpi.edu/doi/pdfplus/10.1021/cb3006402", "note": "", "contentType": "application/pdf", "charset": "", "tags": [], "relations": {}, "dateAdded": "2015-06-23T09:57:09Z", "dateModified": "2015-06-23T09:57:09Z" } }, { "key": "Q9MFT8AA", "version": 4, "library": { "type": "group", "id": 334868, "name": "Built Enviro Microbiome", "links": { "alternate": { "href": "https://www.zotero.org/groups/built_enviro_microbiome", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/334868/items/Q9MFT8AA", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/built_enviro_microbiome/items/Q9MFT8AA", "type": "text/html" } }, "meta": { "createdByUser": { "id": 36430, "username": "mikefortun", "name": "Mike Fortun", "links": { "alternate": { "href": "https://www.zotero.org/mikefortun", "type": "text/html" } } }, "creatorSummary": "Shong et al.", "parsedDate": "2012-10", "numChildren": 2 }, "data": { "key": "Q9MFT8AA", "version": 4, "itemType": "journalArticle", "title": "Towards synthetic microbial consortia for bioprocessing", "creators": [ { "creatorType": "author", "firstName": "Jasmine", "lastName": "Shong" }, { "creatorType": "author", "firstName": "Manuel Rafael", "lastName": "Jimenez Diaz" }, { "creatorType": "author", "firstName": "Cynthia H", "lastName": "Collins" } ], "abstractNote": "The use of microbial consortia for bioprocessing has been limited by our ability to reliably control community composition and function simultaneously. Recent advances in synthetic biology have enabled population-level coordination and control of ecosystem stability and dynamics. Further, new experimental and computational tools for screening and predicting community behavior have also been developed. The integration of synthetic biology with metabolic engineering at the community level is vital to our ability to apply system-level approaches to building and optimizing synthetic consortia for bioprocessing applications. This review details new methods, tools and opportunities that together have the potential to enable a new paradigm of bioprocessing using synthetic microbial consortia.", "publicationTitle": "Current Opinion in Biotechnology", "publisher": "", "place": "", "date": "October 2012", "volume": "23", "issue": "5", "section": "", "partNumber": "", "partTitle": "", "pages": "798-802", "series": "Tissue, cell and pathway engineering", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "Current Opinion in Biotechnology", "DOI": "10.1016/j.copbio.2012.02.001", "citationKey": "", "url": "http://www.sciencedirect.com/science/article/pii/S0958166912000341", "accessDate": "2015-06-23T09:47:10Z", "PMID": "", "PMCID": "", "ISSN": "0958-1669", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "", "libraryCatalog": "ScienceDirect", "callNumber": "", "rights": "", "extra": "00040", "tags": [], "collections": [], "relations": {}, "dateAdded": "2015-06-23T09:47:10Z", "dateModified": "2015-06-23T09:47:11Z" } }, { "key": "QFIECAVS", "version": 4, "library": { "type": "group", "id": 334868, "name": "Built Enviro Microbiome", "links": { "alternate": { "href": "https://www.zotero.org/groups/built_enviro_microbiome", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/334868/items/QFIECAVS", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/built_enviro_microbiome/items/QFIECAVS", "type": "text/html" }, "up": { "href": "https://api.zotero.org/groups/334868/items/Q9MFT8AA", "type": "application/json" } }, "meta": { "createdByUser": { "id": 36430, "username": "mikefortun", "name": "Mike Fortun", "links": { "alternate": { "href": "https://www.zotero.org/mikefortun", "type": "text/html" } } } }, "data": { "key": "QFIECAVS", "version": 4, "parentItem": "Q9MFT8AA", "itemType": "attachment", "linkMode": "linked_url", "title": "ScienceDirect Full Text PDF", "accessDate": "2015-06-23T09:47:10Z", "url": "http://www.sciencedirect.com.libproxy.rpi.edu/science/article/pii/S0958166912000341/pdfft?md5=2399afa285be58ea590834d19dcecf65&pid=1-s2.0-S0958166912000341-main.pdf", "note": "", "contentType": "application/pdf", "charset": "", "tags": [], "relations": {}, "dateAdded": "2015-06-23T09:47:10Z", "dateModified": "2015-06-23T09:47:10Z" } }, { "key": "73INCJ95", "version": 4, "library": { "type": "group", "id": 334868, "name": "Built Enviro Microbiome", "links": { "alternate": { "href": "https://www.zotero.org/groups/built_enviro_microbiome", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/334868/items/73INCJ95", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/built_enviro_microbiome/items/73INCJ95", "type": "text/html" }, "up": { "href": "https://api.zotero.org/groups/334868/items/Q9MFT8AA", "type": "application/json" } }, "meta": { "createdByUser": { "id": 36430, "username": "mikefortun", "name": "Mike Fortun", "links": { "alternate": { "href": "https://www.zotero.org/mikefortun", "type": "text/html" } } } }, "data": { "key": "73INCJ95", "version": 4, "parentItem": "Q9MFT8AA", "itemType": "attachment", "linkMode": "linked_url", "title": "ScienceDirect Snapshot", "accessDate": "2015-06-23T09:47:10Z", "url": "http://www.sciencedirect.com.libproxy.rpi.edu/science/article/pii/S0958166912000341", "note": "", "contentType": "text/html", "charset": "", "tags": [], "relations": {}, "dateAdded": "2015-06-23T09:47:10Z", "dateModified": "2015-06-23T09:47:10Z" } }, { "key": "2KM6IPJ3", "version": 3, "library": { "type": "group", "id": 334868, "name": "Built Enviro Microbiome", "links": { "alternate": { "href": "https://www.zotero.org/groups/built_enviro_microbiome", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/334868/items/2KM6IPJ3", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/built_enviro_microbiome/items/2KM6IPJ3", "type": "text/html" } }, "meta": { "createdByUser": { "id": 36430, "username": "mikefortun", "name": "Mike Fortun", "links": { "alternate": { "href": "https://www.zotero.org/mikefortun", "type": "text/html" } } }, "creatorSummary": "Basu et al.", "parsedDate": "2005-04-28", "numChildren": 2 }, "data": { "key": "2KM6IPJ3", "version": 3, "itemType": "journalArticle", "title": "A synthetic multicellular system for programmed pattern formation", "creators": [ { "creatorType": "author", "firstName": "Subhayu", "lastName": "Basu" }, { "creatorType": "author", "firstName": "Yoram", "lastName": "Gerchman" }, { "creatorType": "author", "firstName": "Cynthia H.", "lastName": "Collins" }, { "creatorType": "author", "firstName": "Frances H.", "lastName": "Arnold" }, { "creatorType": "author", "firstName": "Ron", "lastName": "Weiss" } ], "abstractNote": "Pattern formation is a hallmark of coordinated cell behaviour in both single and multicellular organisms. It typically involves cell–cell communication and intracellular signal processing. Here we show a synthetic multicellular system in which genetically engineered ‘receiver’ cells are programmed to form ring-like patterns of differentiation based on chemical gradients of an acyl-homoserine lactone (AHL) signal that is synthesized by ‘sender’ cells. In receiver cells, ‘band-detect’ gene networks respond to user-defined ranges of AHL concentrations. By fusing different fluorescent proteins as outputs of network variants, an initially undifferentiated ‘lawn’ of receivers is engineered to form a bullseye pattern around a sender colony. Other patterns, such as ellipses and clovers, are achieved by placing senders in different configurations. Experimental and theoretical analyses reveal which kinetic parameters most significantly affect ring development over time. Construction and study of such synthetic multicellular systems can improve our quantitative understanding of naturally occurring developmental processes and may foster applications in tissue engineering, biomaterial fabrication and biosensing.", "publicationTitle": "Nature", "publisher": "", "place": "", "date": "April 28, 2005", "volume": "434", "issue": "7037", "section": "", "partNumber": "", "partTitle": "", "pages": "1130-1134", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "Nature", "DOI": "10.1038/nature03461", "citationKey": "", "url": "http://www.nature.com/nature/journal/v434/n7037/full/nature03461.html", "accessDate": "2015-06-22T23:29:55Z", "PMID": "", "PMCID": "", "ISSN": "0028-0836", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "en", "libraryCatalog": "www.nature.com", "callNumber": "", "rights": "© 2005 Nature Publishing Group", "extra": "00694", "tags": [], "collections": [], "relations": {}, "dateAdded": "2015-06-22T23:29:55Z", "dateModified": "2015-06-22T23:29:56Z" } }, { "key": "WQ4BQKT7", "version": 3, "library": { "type": "group", "id": 334868, "name": "Built Enviro Microbiome", "links": { "alternate": { "href": "https://www.zotero.org/groups/built_enviro_microbiome", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/334868/items/WQ4BQKT7", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/built_enviro_microbiome/items/WQ4BQKT7", "type": "text/html" }, "up": { "href": "https://api.zotero.org/groups/334868/items/2KM6IPJ3", "type": "application/json" } }, "meta": { "createdByUser": { "id": 36430, "username": "mikefortun", "name": "Mike Fortun", "links": { "alternate": { "href": "https://www.zotero.org/mikefortun", "type": "text/html" } } } }, "data": { "key": "WQ4BQKT7", "version": 3, "parentItem": "2KM6IPJ3", "itemType": "attachment", "linkMode": "linked_url", "title": "Full Text PDF", "accessDate": "2015-06-22T23:29:55Z", "url": "http://www.nature.com/nature/journal/v434/n7037/pdf/nature03461.pdf", "note": "", "contentType": "application/pdf", "charset": "", "tags": [], "relations": {}, "dateAdded": "2015-06-22T23:29:55Z", "dateModified": "2015-06-22T23:29:55Z" } }, { "key": "258UJC9S", "version": 3, "library": { "type": "group", "id": 334868, "name": "Built Enviro Microbiome", "links": { "alternate": { "href": "https://www.zotero.org/groups/built_enviro_microbiome", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/334868/items/258UJC9S", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/built_enviro_microbiome/items/258UJC9S", "type": "text/html" }, "up": { "href": "https://api.zotero.org/groups/334868/items/2KM6IPJ3", "type": "application/json" } }, "meta": { "createdByUser": { "id": 36430, "username": "mikefortun", "name": "Mike Fortun", "links": { "alternate": { "href": "https://www.zotero.org/mikefortun", "type": "text/html" } } } }, "data": { "key": "258UJC9S", "version": 3, "parentItem": "2KM6IPJ3", "itemType": "attachment", "linkMode": "linked_url", "title": "Snapshot", "accessDate": "2015-06-22T23:29:55Z", "url": "http://www.nature.com/nature/journal/v434/n7037/full/nature03461.html", "note": "", "contentType": "text/html", "charset": "", "tags": [], "relations": {}, "dateAdded": "2015-06-22T23:29:55Z", "dateModified": "2015-06-22T23:29:55Z" } }, { "key": "3S48WNFW", "version": 7, "library": { "type": "group", "id": 334868, "name": "Built Enviro Microbiome", "links": { "alternate": { "href": "https://www.zotero.org/groups/built_enviro_microbiome", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/334868/items/3S48WNFW", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/built_enviro_microbiome/items/3S48WNFW", "type": "text/html" } }, "meta": { "createdByUser": { "id": 36430, "username": "mikefortun", "name": "Mike Fortun", "links": { "alternate": { "href": "https://www.zotero.org/mikefortun", "type": "text/html" } } }, "creatorSummary": "Mendes et al.", "parsedDate": "2013-09", "numChildren": 1 }, "data": { "key": "3S48WNFW", "version": 7, "itemType": "journalArticle", "title": "The rhizosphere microbiome: significance of plant beneficial, plant pathogenic, and human pathogenic microorganisms", "creators": [ { "creatorType": "author", "firstName": "Rodrigo", "lastName": "Mendes" }, { "creatorType": "author", "firstName": "Paolina", "lastName": "Garbeva" }, { "creatorType": "author", "firstName": "Jos M.", "lastName": "Raaijmakers" } ], "abstractNote": "Microbial communities play a pivotal role in the functioning of plants by influencing their physiology and development. While many members of the rhizosphere microbiome are beneficial to plant growth, also plant pathogenic microorganisms colonize the rhizosphere striving to break through the protective microbial shield and to overcome the innate plant defense mechanisms in order to cause disease. A third group of microorganisms that can be found in the rhizosphere are the true and opportunistic human pathogenic bacteria, which can be carried on or in plant tissue and may cause disease when introduced into debilitated humans. Although the importance of the rhizosphere microbiome for plant growth has been widely recognized, for the vast majority of rhizosphere microorganisms no knowledge exists. To enhance plant growth and health, it is essential to know which microorganism is present in the rhizosphere microbiome and what they are doing. Here, we review the main functions of rhizosphere microorganisms and how they impact on health and disease. We discuss the mechanisms involved in the multitrophic interactions and chemical dialogues that occur in the rhizosphere. Finally, we highlight several strategies to redirect or reshape the rhizosphere microbiome in favor of microorganisms that are beneficial to plant growth and health.", "publicationTitle": "FEMS microbiology reviews", "publisher": "", "place": "", "date": "Sep 2013", "volume": "37", "issue": "5", "section": "", "partNumber": "", "partTitle": "", "pages": "634-663", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "FEMS Microbiol. Rev.", "DOI": "10.1111/1574-6976.12028", "citationKey": "", "url": "", "accessDate": "", "PMID": "23790204", "PMCID": "", "ISSN": "1574-6976", "archive": "", "archiveLocation": "", "shortTitle": "The rhizosphere microbiome", "language": "eng", "libraryCatalog": "NCBI PubMed", "callNumber": "", "rights": "", "extra": "00058", "tags": [], "collections": [], "relations": {}, "dateAdded": "2015-03-06T17:54:17Z", "dateModified": "2015-03-06T17:54:18Z" } }, { "key": "JIFGBQ7T", "version": 2, "library": { "type": "group", "id": 334868, "name": "Built Enviro Microbiome", "links": { "alternate": { "href": "https://www.zotero.org/groups/built_enviro_microbiome", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/334868/items/JIFGBQ7T", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/built_enviro_microbiome/items/JIFGBQ7T", "type": "text/html" }, "up": { "href": "https://api.zotero.org/groups/334868/items/3S48WNFW", "type": "application/json" } }, "meta": { "createdByUser": { "id": 36430, "username": "mikefortun", "name": "Mike Fortun", "links": { "alternate": { "href": "https://www.zotero.org/mikefortun", "type": "text/html" } } } }, "data": { "key": "JIFGBQ7T", "version": 2, "parentItem": "3S48WNFW", "itemType": "attachment", "linkMode": "linked_url", "title": "PubMed entry", "accessDate": "2015-03-06T17:54:17Z", "url": "http://www.ncbi.nlm.nih.gov/pubmed/23790204", "note": "", "contentType": "text/html", "charset": "", "tags": [], "relations": {}, "dateAdded": "2015-03-06T17:54:17Z", "dateModified": "2015-03-06T17:54:17Z" } } ]