[ { "key": "5MQGZPMZ", "version": 179, "library": { "type": "group", "id": 316688, "name": "Regenerative/PRP", "links": { "alternate": { "href": "https://www.zotero.org/groups/regenerativeprp", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/316688/items/5MQGZPMZ", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/regenerativeprp/items/5MQGZPMZ", "type": "text/html" }, "up": { "href": "https://api.zotero.org/groups/316688/items/9EYAUTQI", "type": "application/json" } }, "meta": { "createdByUser": { "id": 472710, "username": "Lancelgoetz", "name": "Lance L. Goetz, M.D.", "links": { "alternate": { "href": "https://www.zotero.org/lancelgoetz", "type": "text/html" } } }, "numChildren": 0 }, "data": { "key": "5MQGZPMZ", "version": 179, "parentItem": "9EYAUTQI", "itemType": "attachment", "linkMode": "imported_url", "title": "Full Text", "accessDate": "2020-12-23T16:51:18Z", "url": "https://bmcmusculoskeletdisord.biomedcentral.com/track/pdf/10.1186/s12891-020-3120-0", "note": "", "contentType": "application/pdf", "charset": "", "filename": "Li et al. - 2020 - Profiling of inflammatory mediators in the synovia.pdf", "md5": "4e052fd08d8952223a14583a3d5d6b0f", "mtime": 1608742278343, "tags": [], "relations": {}, "dateAdded": "2020-12-23T16:51:18Z", "dateModified": "2020-12-23T16:51:18Z" } }, { "key": "XCYQN8DE", "version": 178, "library": { "type": "group", "id": 316688, "name": "Regenerative/PRP", "links": { "alternate": { "href": "https://www.zotero.org/groups/regenerativeprp", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/316688/items/XCYQN8DE", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/regenerativeprp/items/XCYQN8DE", "type": "text/html" }, "up": { "href": "https://api.zotero.org/groups/316688/items/9EYAUTQI", "type": "application/json" } }, "meta": { "createdByUser": { "id": 472710, "username": "Lancelgoetz", "name": "Lance L. Goetz, M.D.", "links": { "alternate": { "href": "https://www.zotero.org/lancelgoetz", "type": "text/html" } } } }, "data": { "key": "XCYQN8DE", "version": 178, "parentItem": "9EYAUTQI", "itemType": "attachment", "linkMode": "linked_url", "title": "PubMed entry", "accessDate": "2020-12-23T16:51:13Z", "url": "http://www.ncbi.nlm.nih.gov/pubmed/32059658", "note": "", "contentType": "text/html", "charset": "", "tags": [], "relations": {}, "dateAdded": "2020-12-23T16:51:13Z", "dateModified": "2020-12-23T16:51:13Z" } }, { "key": "9EYAUTQI", "version": 178, "library": { "type": "group", "id": 316688, "name": "Regenerative/PRP", "links": { "alternate": { "href": "https://www.zotero.org/groups/regenerativeprp", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/316688/items/9EYAUTQI", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/regenerativeprp/items/9EYAUTQI", "type": "text/html" } }, "meta": { "createdByUser": { "id": 472710, "username": "Lancelgoetz", "name": "Lance L. Goetz, M.D.", "links": { "alternate": { "href": "https://www.zotero.org/lancelgoetz", "type": "text/html" } } }, "creatorSummary": "Li et al.", "parsedDate": "2020-02-14", "numChildren": 2 }, "data": { "key": "9EYAUTQI", "version": 178, "itemType": "journalArticle", "title": "Profiling of inflammatory mediators in the synovial fluid related to pain in knee osteoarthritis", "creators": [ { "creatorType": "author", "firstName": "Li", "lastName": "Li" }, { "creatorType": "author", "firstName": "Zhenxing", "lastName": "Li" }, { "creatorType": "author", "firstName": "Yuyan", "lastName": "Li" }, { "creatorType": "author", "firstName": "Xi", "lastName": "Hu" }, { "creatorType": "author", "firstName": "Yu", "lastName": "Zhang" }, { "creatorType": "author", "firstName": "Pei", "lastName": "Fan" } ], "abstractNote": "BACKGROUND: Inflammatory mediators in the synovial fluid (SF) play critical roles in the initiation and development of pain in knee osteoarthritis (KOA). However, data for inflammatory marker expression are conflicting, and the role of SF inflammatory mediators in neuropathic pain is not clear. Therefore, the aim of this study was to identify SF inflammatory mediators associated with nociceptive and neuropathic pain in KOA.\nMETHODS: Levels of IL-1β, IL-6, TNF-α, macrophage colony-stimulating factor, MMP-3, MMP-13, metalloproteinase with thrombospondin motifs 5, calcitonin gene-related peptide, neuropeptide Y, substance P and bradykinin were measured using enzyme-linked immunosorbent assays in 86 patients. Nociceptive pain was assessed using the numeric rating scale (NRS), visual analog scale (VAS) and Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) pain score. Neuropathic pain was determined using the PainDETECT questionnaire. Moreover, knee function was evaluated by the WOMAC score and range of motion (ROM) assessments. Radiological grade was defined using the Kellgren-Lawrence (K-L) grading scale.\nRESULTS: Pain scores measured using different methods correlated highly with each other. A worse K-L grade and knee function were associated with worse pain. Expression of IL-1β and IL-6 was increased in the early stage compared with the late stage. The NRS score correlated positively with age, K-L grade, and the WOMAC score and negatively with ROM and TNF-α expression. The VAS correlated positively with age, K-L grade, and the WOMAC score but negatively with ROM and levels of IL-1β, IL-6 and TNF-α. The WOMAC pain score did not correlate with any of the inflammatory mediators measured; it correlated only with ROM. The PainDETECT score correlated only with the WOMAC score. Expression of other inflammatory mediators did not correlate with any of the pain scores.\nCONCLUSIONS: IL-1β, IL-6 and TNF-α play critical roles in pain in the early stage of KOA and correlate with pain. The catabolic enzymes and neuropeptides measured do not correlate with nociceptive and neuropathic pain. New biomarkers related to pain in the late stage need to be further investigated.", "publicationTitle": "BMC musculoskeletal disorders", "publisher": "", "place": "", "date": "2020-02-14", "volume": "21", "issue": "1", "section": "", "partNumber": "", "partTitle": "", "pages": "99", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "BMC Musculoskelet Disord", "DOI": "10.1186/s12891-020-3120-0", "citationKey": "", "url": "", "accessDate": "", "PMID": "", "PMCID": "", "ISSN": "1471-2474", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "eng", "libraryCatalog": "PubMed", "callNumber": "", "rights": "", "extra": "PMID: 32059658\nPMCID: PMC7023718", "tags": [ { "tag": "Aged", "type": 1 }, { "tag": "Aged, 80 and over", "type": 1 }, { "tag": "Biomarkers", "type": 1 }, { "tag": "Cytokines", "type": 1 }, { "tag": "Enzyme-Linked Immunosorbent Assay", "type": 1 }, { "tag": "Female", "type": 1 }, { "tag": "Humans", "type": 1 }, { "tag": "Inflammation Mediators", "type": 1 }, { "tag": "Inflammation mediators", "type": 1 }, { "tag": "Knee Joint", "type": 1 }, { "tag": "Male", "type": 1 }, { "tag": "Metalloproteases", "type": 1 }, { "tag": "Middle Aged", "type": 1 }, { "tag": "Neuralgia", "type": 1 }, { "tag": "Osteoarthritis, Knee", "type": 1 }, { "tag": "Osteoarthritis, knee", "type": 1 }, { "tag": "Pain", "type": 1 }, { "tag": "Pain Measurement", "type": 1 }, { "tag": "Radiography", "type": 1 }, { "tag": "Range of Motion, Articular", "type": 1 }, { "tag": "Severity of Illness Index", "type": 1 }, { "tag": "Surveys and Questionnaires", "type": 1 }, { "tag": "Synovial Fluid", "type": 1 }, { "tag": "Synovial fluid", "type": 1 }, { "tag": "Visual Analog Scale", "type": 1 } ], "collections": [], "relations": {}, "dateAdded": "2020-12-23T16:51:12Z", "dateModified": "2020-12-23T16:51:12Z" } }, { "key": "9YPMUASU", "version": 176, "library": { "type": "group", "id": 316688, "name": "Regenerative/PRP", "links": { "alternate": { "href": "https://www.zotero.org/groups/regenerativeprp", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/316688/items/9YPMUASU", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/regenerativeprp/items/9YPMUASU", "type": "text/html" }, "up": { "href": "https://api.zotero.org/groups/316688/items/YWT4EZVL", "type": "application/json" } }, "meta": { "createdByUser": { "id": 472710, "username": "Lancelgoetz", "name": "Lance L. Goetz, M.D.", "links": { "alternate": { "href": "https://www.zotero.org/lancelgoetz", "type": "text/html" } } } }, "data": { "key": "9YPMUASU", "version": 176, "parentItem": "YWT4EZVL", "itemType": "attachment", "linkMode": "linked_url", "title": "PubMed entry", "accessDate": "2020-12-23T16:29:28Z", "url": "http://www.ncbi.nlm.nih.gov/pubmed/28910372", "note": "", "contentType": "text/html", "charset": "", "tags": [], "relations": {}, "dateAdded": "2020-12-23T16:29:28Z", "dateModified": "2020-12-23T16:29:28Z" } }, { "key": "YWT4EZVL", "version": 176, "library": { "type": "group", "id": 316688, "name": "Regenerative/PRP", "links": { "alternate": { "href": "https://www.zotero.org/groups/regenerativeprp", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/316688/items/YWT4EZVL", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/regenerativeprp/items/YWT4EZVL", "type": "text/html" } }, "meta": { "createdByUser": { "id": 472710, "username": "Lancelgoetz", "name": "Lance L. Goetz, M.D.", "links": { "alternate": { "href": "https://www.zotero.org/lancelgoetz", "type": "text/html" } } }, "creatorSummary": "Das Gupta et al.", "parsedDate": "2017", "numChildren": 1 }, "data": { "key": "YWT4EZVL", "version": 176, "itemType": "journalArticle", "title": "Correlation of serum cartilage oligomeric matrix protein (COMP) and interleukin-16 (IL-16) levels with disease severity in primary knee osteoarthritis: A pilot study in a Malaysian population", "creators": [ { "creatorType": "author", "firstName": "Esha", "lastName": "Das Gupta" }, { "creatorType": "author", "firstName": "Wei Ren", "lastName": "Ng" }, { "creatorType": "author", "firstName": "Shew Fung", "lastName": "Wong" }, { "creatorType": "author", "firstName": "Abdul Kareem", "lastName": "Bhurhanudeen" }, { "creatorType": "author", "firstName": "Swan Sim", "lastName": "Yeap" } ], "abstractNote": "OBJECTIVE: The aim of this study was to investigate the correlations between serum cartilage oligomeric matrix protein (COMP), interleukin-16 (IL-16) and different grades of knee osteoarthritis (KOA) in Malaysian subjects.\nMETHODS: Ninety subjects were recruited comprising 30 with Kellgren-Lawrence (K-L) grade 2 KOA, 27 with K-L grade 3 KOA, 7 with grade 4 KOA, and 30 healthy controls. All subjects completed the Western Ontario and McMaster Universities Arthritis Index (WOMAC) questionnaire. Serum COMP and IL-16 levels were measured using ELISA and their values log transformed to ensure a normal distribution.\nRESULTS: There was no significant differences in levels of log serum COMP and IL-16 between healthy controls and KOA patients. There were no significant differences in the log serum COMP and IL-16 levels within the different K-L grades in the KOA patients. In KOA patients, log serum IL-16 levels significantly correlated with the WOMAC score (p = 0.001) and its subscales, pain (p = 0.005), stiffness (p = 0.019) and physical function (p<0.0001). Serum IL-16 levels were significantly higher in Malaysian Indians compared to Malays and Chinese (p = 0.024).\nCONCLUSIONS: In this multi-ethnic Malaysian population, there was no difference in serum COMP and IL-16 levels between healthy controls and patients with KOA, nor was there any difference in serum COMP or IL-16 levels across the various K-L grades of KOA. However, there were significant inter-racial differences in serum IL-16 levels.", "publicationTitle": "PloS One", "publisher": "", "place": "", "date": "2017", "volume": "12", "issue": "9", "section": "", "partNumber": "", "partTitle": "", "pages": "e0184802", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "PLoS One", "DOI": "10.1371/journal.pone.0184802", "citationKey": "", "url": "", "accessDate": "", "PMID": "", "PMCID": "", "ISSN": "1932-6203", "archive": "", "archiveLocation": "", "shortTitle": "Correlation of serum cartilage oligomeric matrix protein (COMP) and interleukin-16 (IL-16) levels with disease severity in primary knee osteoarthritis", "language": "eng", "libraryCatalog": "PubMed", "callNumber": "", "rights": "", "extra": "PMID: 28910372\nPMCID: PMC5599033", "tags": [ { "tag": "Aged", "type": 1 }, { "tag": "Asian Continental Ancestry Group", "type": 1 }, { "tag": "Cartilage Oligomeric Matrix Protein", "type": 1 }, { "tag": "Female", "type": 1 }, { "tag": "Humans", "type": 1 }, { "tag": "Interleukin-6", "type": 1 }, { "tag": "Malaysia", "type": 1 }, { "tag": "Male", "type": 1 }, { "tag": "Middle Aged", "type": 1 }, { "tag": "Osteoarthritis, Knee", "type": 1 }, { "tag": "Pilot Projects", "type": 1 }, { "tag": "Severity of Illness Index", "type": 1 } ], "collections": [], "relations": {}, "dateAdded": "2020-12-23T16:29:28Z", "dateModified": "2020-12-23T16:29:28Z" } }, { "key": "KRCPY5VI", "version": 174, "library": { "type": "group", "id": 316688, "name": "Regenerative/PRP", "links": { "alternate": { "href": "https://www.zotero.org/groups/regenerativeprp", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/316688/items/KRCPY5VI", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/regenerativeprp/items/KRCPY5VI", "type": "text/html" }, "up": { "href": "https://api.zotero.org/groups/316688/items/Z9JSH9P8", "type": "application/json" } }, "meta": { "createdByUser": { "id": 472710, "username": "Lancelgoetz", "name": "Lance L. Goetz, M.D.", "links": { "alternate": { "href": "https://www.zotero.org/lancelgoetz", "type": "text/html" } } }, "numChildren": 0 }, "data": { "key": "KRCPY5VI", "version": 174, "parentItem": "Z9JSH9P8", "itemType": "attachment", "linkMode": "imported_url", "title": "Full Text", "accessDate": "2020-12-23T16:24:13Z", "url": "https://downloads.hindawi.com/journals/prt/2013/165967.pdf", "note": "", "contentType": "application/pdf", "charset": "", "filename": "Raeissadat et al. - 2013 - The effect of platelet-rich plasma on pain, functi.pdf", "md5": "ddbff393b4177b8c93ab4f44ef1ec958", "mtime": 1608740653731, "tags": [], "relations": {}, "dateAdded": "2020-12-23T16:24:13Z", "dateModified": "2020-12-23T16:24:13Z" } }, { "key": "R5M7LPIC", "version": 173, "library": { "type": "group", "id": 316688, "name": "Regenerative/PRP", "links": { "alternate": { "href": "https://www.zotero.org/groups/regenerativeprp", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/316688/items/R5M7LPIC", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/regenerativeprp/items/R5M7LPIC", "type": "text/html" }, "up": { "href": "https://api.zotero.org/groups/316688/items/Z9JSH9P8", "type": "application/json" } }, "meta": { "createdByUser": { "id": 472710, "username": "Lancelgoetz", "name": "Lance L. Goetz, M.D.", "links": { "alternate": { "href": "https://www.zotero.org/lancelgoetz", "type": "text/html" } } } }, "data": { "key": "R5M7LPIC", "version": 173, "parentItem": "Z9JSH9P8", "itemType": "attachment", "linkMode": "linked_url", "title": "PubMed entry", "accessDate": "2020-12-23T16:24:08Z", "url": "http://www.ncbi.nlm.nih.gov/pubmed/24386565", "note": "", "contentType": "text/html", "charset": "", "tags": [], "relations": {}, "dateAdded": "2020-12-23T16:24:08Z", "dateModified": "2020-12-23T16:24:08Z" } }, { "key": "Z9JSH9P8", "version": 173, "library": { "type": "group", "id": 316688, "name": "Regenerative/PRP", "links": { "alternate": { "href": "https://www.zotero.org/groups/regenerativeprp", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/316688/items/Z9JSH9P8", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/regenerativeprp/items/Z9JSH9P8", "type": "text/html" } }, "meta": { "createdByUser": { "id": 472710, "username": "Lancelgoetz", "name": "Lance L. Goetz, M.D.", "links": { "alternate": { "href": "https://www.zotero.org/lancelgoetz", "type": "text/html" } } }, "creatorSummary": "Raeissadat et al.", "parsedDate": "2013", "numChildren": 2 }, "data": { "key": "Z9JSH9P8", "version": 173, "itemType": "journalArticle", "title": "The effect of platelet-rich plasma on pain, function, and quality of life of patients with knee osteoarthritis", "creators": [ { "creatorType": "author", "firstName": "Seyed Ahmad", "lastName": "Raeissadat" }, { "creatorType": "author", "firstName": "Seyed Mansoor", "lastName": "Rayegani" }, { "creatorType": "author", "firstName": "Marzieh", "lastName": "Babaee" }, { "creatorType": "author", "firstName": "Elham", "lastName": "Ghorbani" } ], "abstractNote": "Background. New studies in the management of knee osteoarthritis have focused on modern therapeutic methods stimulating cartilage healing process. In the present study, we evaluated the effects of 2 courses of leucocyte-rich PRP (LR-PRP) injections on patients' QOL and functions and also the relationship between the PRP concentration and mentioned variables. Material and Methods. Sixty-five patients were evaluated. For each participant, WOMAC and the native (Farsi) edition of the SF-36 questionnaire were filled. Two courses of LR-PRP injections with 4-week interval were used. After 6 months, SF-36 and WOMAC questionnaires were filled again for each patient. Results. 60 patients were included in the final analysis. The mean platelet concentrations and white blood cell in PRP was 5-fold increase and 220 per microliter, respectively. The mean total WOMAC revealed significant change (P = 0.001). In SF-36, the mean changes of 2 major physical and mental domains were meaningful (P = 0.001). Discussion. In our study, 2 injections of PRP, with 4-week interval, improved the pain, stiffness, and functional capacity. Improvements in QOL (both PCS and MCS) were meaningful after injections. These changes were more significant in physical domains. PRP injection may be an alternative therapy in selective patients resistant to current nonsurgical treatments of knee osteoarthritis.", "publicationTitle": "Pain Research and Treatment", "publisher": "", "place": "", "date": "2013", "volume": "2013", "issue": "", "section": "", "partNumber": "", "partTitle": "", "pages": "165967", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "Pain Res Treat", "DOI": "10.1155/2013/165967", "citationKey": "", "url": "", "accessDate": "", "PMID": "", "PMCID": "", "ISSN": "2090-1542", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "eng", "libraryCatalog": "PubMed", "callNumber": "", "rights": "", "extra": "PMID: 24386565\nPMCID: PMC3872432", "tags": [], "collections": [], "relations": {}, "dateAdded": "2020-12-23T16:24:07Z", "dateModified": "2020-12-23T16:24:07Z" } }, { "key": "TKZZX4TN", "version": 172, "library": { "type": "group", "id": 316688, "name": "Regenerative/PRP", "links": { "alternate": { "href": "https://www.zotero.org/groups/regenerativeprp", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/316688/items/TKZZX4TN", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/regenerativeprp/items/TKZZX4TN", "type": "text/html" }, "up": { "href": "https://api.zotero.org/groups/316688/items/6MD8AEZ8", "type": "application/json" } }, "meta": { "createdByUser": { "id": 472710, "username": "Lancelgoetz", "name": "Lance L. Goetz, M.D.", "links": { "alternate": { "href": "https://www.zotero.org/lancelgoetz", "type": "text/html" } } } }, "data": { "key": "TKZZX4TN", "version": 172, "parentItem": "6MD8AEZ8", "itemType": "attachment", "linkMode": "linked_url", "title": "PubMed entry", "accessDate": "2020-12-23T16:22:08Z", "url": "http://www.ncbi.nlm.nih.gov/pubmed/23299850", "note": "", "contentType": "text/html", "charset": "", "tags": [], "relations": {}, "dateAdded": "2020-12-23T16:22:08Z", "dateModified": "2020-12-23T16:22:08Z" } }, { "key": "6MD8AEZ8", "version": 172, "library": { "type": "group", "id": 316688, "name": "Regenerative/PRP", "links": { "alternate": { "href": "https://www.zotero.org/groups/regenerativeprp", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/316688/items/6MD8AEZ8", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/regenerativeprp/items/6MD8AEZ8", "type": "text/html" } }, "meta": { "createdByUser": { "id": 472710, "username": "Lancelgoetz", "name": "Lance L. Goetz, M.D.", "links": { "alternate": { "href": "https://www.zotero.org/lancelgoetz", "type": "text/html" } } }, "creatorSummary": "Patel et al.", "parsedDate": "2013-02", "numChildren": 1 }, "data": { "key": "6MD8AEZ8", "version": 172, "itemType": "journalArticle", "title": "Treatment with platelet-rich plasma is more effective than placebo for knee osteoarthritis: a prospective, double-blind, randomized trial", "creators": [ { "creatorType": "author", "firstName": "Sandeep", "lastName": "Patel" }, { "creatorType": "author", "firstName": "Mandeep S.", "lastName": "Dhillon" }, { "creatorType": "author", "firstName": "Sameer", "lastName": "Aggarwal" }, { "creatorType": "author", "firstName": "Neelam", "lastName": "Marwaha" }, { "creatorType": "author", "firstName": "Ashish", "lastName": "Jain" } ], "abstractNote": "BACKGROUND: Specific growth factors have been proposed as therapeutic proteins for cartilage repair.\nHYPOTHESIS: Platelet-rich plasma (PRP) provides symptomatic relief in early osteoarthritis (OA) of the knee.\nSTUDY DESIGN: Randomized controlled trial; Level of evidence, 1.\nMETHODS: A total of 78 patients (156 knees) with bilateral OA were divided randomly into 3 groups. Group A (52 knees) received a single injection of PRP, group B (50 knees) received 2 injections of PRP 3 weeks apart, and group C (46 knees) received a single injection of normal saline. White blood cell (WBC)-filtered PRP with a platelet count 3 times that of baseline (PRP type 4B) was administered in all. All the groups were homogeneous and comparable in baseline characteristics. Clinical outcome was evaluated using the Western Ontario and McMaster Universities Arthritis Index (WOMAC) questionnaire before treatment and at 6 weeks, 3 months, and 6 months after treatment. They were also evaluated for pain by a visual analog scale, and overall satisfaction with the procedure and complications were noted.\nRESULTS: Statistically significant improvement in all WOMAC parameters was noted in groups A and B within 2 to 3 weeks and lasting until the final follow-up at 6 months, with slight worsening at the 6-month follow-up. The mean WOMAC scores (pain, stiffness, physical function, and total score) for group A at baseline were 10.18, 3.12, 36.56, and 49.86, respectively, and at final follow-up were 5.00, 2.10, 20.08, and 27.18, respectively, showing significant improvement. Similar improvement was noted in group B (mean WOMAC scores at baseline: 10.62, 3.50, 39.10, and 53.20, respectively; mean WOMAC scores at final follow-up: 6.18, 1.88, 22.40, and 30.48, respectively). In group C, the mean WOMAC scores deteriorated from baseline (9.04, 2.70, 33.80, and 45.54, respectively) to final follow-up (10.87, 2.76, 39.46, and 53.09, respectively). The 3 groups were compared with each other, and no improvement was noted in group C as compared with groups A and B (P < .001). There was no difference between groups A and B, and there was no influence of age, sex, weight, or body mass index on the outcome. Knees with Ahlback grade 1 fared better than those with grade 2. Mild complications such as nausea and dizziness, which were of short duration, were observed in 6 patients (22.2%) in group A and 11 patients (44%) in group B.\nCONCLUSION: A single dose of WBC-filtered PRP in concentrations of 10 times the normal amount is as effective as 2 injections to alleviate symptoms in early knee OA. The results, however, deteriorate after 6 months. Both groups treated with PRP had better results than did the group injected with saline only.", "publicationTitle": "The American Journal of Sports Medicine", "publisher": "", "place": "", "date": "2013-02", "volume": "41", "issue": "2", "section": "", "partNumber": "", "partTitle": "", "pages": "356-364", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "Am J Sports Med", "DOI": "10.1177/0363546512471299", "citationKey": "", "url": "", "accessDate": "", "PMID": "", "PMCID": "", "ISSN": "1552-3365", "archive": "", "archiveLocation": "", "shortTitle": "Treatment with platelet-rich plasma is more effective than placebo for knee osteoarthritis", "language": "eng", "libraryCatalog": "PubMed", "callNumber": "", "rights": "", "extra": "PMID: 23299850", "tags": [ { "tag": "Adult", "type": 1 }, { "tag": "Aged", "type": 1 }, { "tag": "Aged, 80 and over", "type": 1 }, { "tag": "Double-Blind Method", "type": 1 }, { "tag": "Female", "type": 1 }, { "tag": "Humans", "type": 1 }, { "tag": "Male", "type": 1 }, { "tag": "Middle Aged", "type": 1 }, { "tag": "Osteoarthritis, Knee", "type": 1 }, { "tag": "Platelet-Rich Plasma", "type": 1 }, { "tag": "Prospective Studies", "type": 1 }, { "tag": "Treatment Outcome", "type": 1 } ], "collections": [], "relations": {}, "dateAdded": "2020-12-23T16:22:07Z", "dateModified": "2020-12-23T16:22:07Z" } }, { "key": "YBJWSTY7", "version": 170, "library": { "type": "group", "id": 316688, "name": "Regenerative/PRP", "links": { "alternate": { "href": "https://www.zotero.org/groups/regenerativeprp", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/316688/items/YBJWSTY7", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/regenerativeprp/items/YBJWSTY7", "type": "text/html" }, "up": { "href": "https://api.zotero.org/groups/316688/items/RF83H2QI", "type": "application/json" } }, "meta": { "createdByUser": { "id": 472710, "username": "Lancelgoetz", "name": "Lance L. Goetz, M.D.", "links": { "alternate": { "href": "https://www.zotero.org/lancelgoetz", "type": "text/html" } } }, "numChildren": 0 }, "data": { "key": "YBJWSTY7", "version": 170, "parentItem": "RF83H2QI", "itemType": "attachment", "linkMode": "imported_url", "title": "Full Text", "accessDate": "2020-12-23T15:36:20Z", "url": "https://downloads.hindawi.com/journals/sci/2012/953510.pdf", "note": "", "contentType": "application/pdf", "charset": "", "filename": "Pascual-Garrido et al. - 2012 - Treatment of Chronic Patellar Tendinopathy with Au.pdf", "md5": "cf9afc18989c0d7f4f1377e64c3c52a9", "mtime": 1608737780563, "tags": [], "relations": {}, "dateAdded": "2020-12-23T15:36:20Z", "dateModified": "2020-12-23T15:36:20Z" } }, { "key": "RF83H2QI", "version": 169, "library": { "type": "group", "id": 316688, "name": "Regenerative/PRP", "links": { "alternate": { "href": "https://www.zotero.org/groups/regenerativeprp", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/316688/items/RF83H2QI", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/regenerativeprp/items/RF83H2QI", "type": "text/html" } }, "meta": { "createdByUser": { "id": 472710, "username": "Lancelgoetz", "name": "Lance L. Goetz, M.D.", "links": { "alternate": { "href": "https://www.zotero.org/lancelgoetz", "type": "text/html" } } }, "creatorSummary": "Pascual-Garrido et al.", "parsedDate": "2012", "numChildren": 1 }, "data": { "key": "RF83H2QI", "version": 169, "itemType": "journalArticle", "title": "Treatment of Chronic Patellar Tendinopathy with Autologous Bone Marrow Stem Cells: A 5-Year-Followup", "creators": [ { "creatorType": "author", "firstName": "Cecilia", "lastName": "Pascual-Garrido" }, { "creatorType": "author", "firstName": "A.", "lastName": "Rolón" }, { "creatorType": "author", "firstName": "A.", "lastName": "Makino" } ], "abstractNote": "The purpose of this study is to determine if patients with chronic patellar tendinopathy will improve clinically after the inoculation of bone marrow mononuclear cells (BM-MNCs). Eight patients with chronic patellar tendinopathy were included. Patients averaged 24 years old (range 14–35). All patients were refractory to conservative treatment for at least 6 months before the procedure. BM-MNCs were harvested from the iliac bone crest and inoculated under ultrasound guide in the patellar tendon lesion. Improvement was assessed through established clinical scores and ultrasound. At 5-year followup, statistically significant improvement was seen for most clinical scores. Seven of eight patients said they would have the procedure again if they had the same problem in the opposite knee and were completely satisfied with the procedure. Seven of 8 patients thought that the results of the procedure were excellent. According to our results, inoculation of BM-MNCs could be considered as a potential therapy for those patients with chronic patellar tendinopathy refractory to nonoperative treatments.", "publicationTitle": "Stem Cells International", "publisher": "", "place": "", "date": "2012", "volume": "2012", "issue": "", "section": "", "partNumber": "", "partTitle": "", "pages": "1-5", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "Stem Cells International", "DOI": "10.1155/2012/953510", "citationKey": "", "url": "http://www.hindawi.com/journals/sci/2012/953510/", "accessDate": "2020-12-23T15:36:16Z", "PMID": "", "PMCID": "", "ISSN": "1687-966X, 1687-9678", "archive": "", "archiveLocation": "", "shortTitle": "Treatment of Chronic Patellar Tendinopathy with Autologous Bone Marrow Stem Cells", "language": "en", "libraryCatalog": "DOI.org (Crossref)", "callNumber": "", "rights": "", "extra": "", "tags": [], "collections": [], "relations": {}, "dateAdded": "2020-12-23T15:36:16Z", "dateModified": "2020-12-23T15:36:16Z" } }, { "key": "G5UI7EFA", "version": 168, "library": { "type": "group", "id": 316688, "name": "Regenerative/PRP", "links": { "alternate": { "href": "https://www.zotero.org/groups/regenerativeprp", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/316688/items/G5UI7EFA", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/regenerativeprp/items/G5UI7EFA", "type": "text/html" } }, "meta": { "createdByUser": { "id": 472710, "username": "Lancelgoetz", "name": "Lance L. Goetz, M.D.", "links": { "alternate": { "href": "https://www.zotero.org/lancelgoetz", "type": "text/html" } } }, "creatorSummary": "Pettine et al.", "parsedDate": "2017", "numChildren": 0 }, "data": { "key": "G5UI7EFA", "version": 168, "itemType": "journalArticle", "title": "Autologous bone marrow concentrate intradiscal injection for the treatment of degenerative disc disease with three-year follow-up", "creators": [ { "creatorType": "author", "firstName": "Kenneth A.", "lastName": "Pettine" }, { "creatorType": "author", "firstName": "Richard K.", "lastName": "Suzuki" }, { "creatorType": "author", "firstName": "Theodore T.", "lastName": "Sand" }, { "creatorType": "author", "firstName": "Matthew B.", "lastName": "Murphy" } ], "abstractNote": "", "publicationTitle": "International Orthopaedics", "publisher": "", "place": "", "date": "10/2017", "volume": "41", "issue": "10", "section": "", "partNumber": "", "partTitle": "", "pages": "2097-2103", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "International Orthopaedics (SICOT)", "DOI": "10.1007/s00264-017-3560-9", "citationKey": "", "url": "http://link.springer.com/10.1007/s00264-017-3560-9", "accessDate": "2020-12-23T15:35:36Z", "PMID": "", "PMCID": "", "ISSN": "0341-2695, 1432-5195", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "en", "libraryCatalog": "DOI.org (Crossref)", "callNumber": "", "rights": "", "extra": "", "tags": [], "collections": [], "relations": {}, "dateAdded": "2020-12-23T15:35:36Z", "dateModified": "2020-12-23T15:35:36Z" } }, { "key": "33UVC49J", "version": 167, "library": { "type": "group", "id": 316688, "name": "Regenerative/PRP", "links": { "alternate": { "href": "https://www.zotero.org/groups/regenerativeprp", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/316688/items/33UVC49J", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/regenerativeprp/items/33UVC49J", "type": "text/html" } }, "meta": { "createdByUser": { "id": 472710, "username": "Lancelgoetz", "name": "Lance L. Goetz, M.D.", "links": { "alternate": { "href": "https://www.zotero.org/lancelgoetz", "type": "text/html" } } }, "creatorSummary": "Hernigou et al.", "parsedDate": "2020-07-02", "numChildren": 0 }, "data": { "key": "33UVC49J", "version": 167, "itemType": "journalArticle", "title": "Subchondral bone or intra-articular injection of bone marrow concentrate mesenchymal stem cells in bilateral knee osteoarthritis: what better postpone knee arthroplasty at fifteen years? A randomized study", "creators": [ { "creatorType": "author", "firstName": "Philippe", "lastName": "Hernigou" }, { "creatorType": "author", "firstName": "Charlie", "lastName": "Bouthors" }, { "creatorType": "author", "firstName": "Claire", "lastName": "Bastard" }, { "creatorType": "author", "firstName": "Charles Henri", "lastName": "Flouzat Lachaniette" }, { "creatorType": "author", "firstName": "Helene", "lastName": "Rouard" }, { "creatorType": "author", "firstName": "Arnaud", "lastName": "Dubory" } ], "abstractNote": "", "publicationTitle": "International Orthopaedics", "publisher": "", "place": "", "date": "2020-7-2", "volume": "", "issue": "", "section": "", "partNumber": "", "partTitle": "", "pages": "", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "International Orthopaedics (SICOT)", "DOI": "10.1007/s00264-020-04687-7", "citationKey": "", "url": "http://link.springer.com/10.1007/s00264-020-04687-7", "accessDate": "2020-12-23T15:35:01Z", "PMID": "", "PMCID": "", "ISSN": "0341-2695, 1432-5195", "archive": "", "archiveLocation": "", "shortTitle": "Subchondral bone or intra-articular injection of bone marrow concentrate mesenchymal stem cells in bilateral knee osteoarthritis", "language": "en", "libraryCatalog": "DOI.org (Crossref)", "callNumber": "", "rights": "", "extra": "", "tags": [], "collections": [], "relations": {}, "dateAdded": "2020-12-23T15:35:01Z", "dateModified": "2020-12-23T15:35:01Z" } }, { "key": "33LHX4KL", "version": 166, "library": { "type": "group", "id": 316688, "name": "Regenerative/PRP", "links": { "alternate": { "href": "https://www.zotero.org/groups/regenerativeprp", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/316688/items/33LHX4KL", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/regenerativeprp/items/33LHX4KL", "type": "text/html" } }, "meta": { "createdByUser": { "id": 472710, "username": "Lancelgoetz", "name": "Lance L. 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Goetz, M.D.", "links": { "alternate": { "href": "https://www.zotero.org/lancelgoetz", "type": "text/html" } } }, "numChildren": 0 }, "data": { "key": "SQT99JBR", "version": 164, "parentItem": "BNNTJ98D", "itemType": "attachment", "linkMode": "imported_url", "title": "Full Text", "accessDate": "2020-09-18T19:53:45Z", "url": "https://journals.sagepub.com/doi/pdf/10.3727/096368916X693464", "note": "", "contentType": "application/pdf", "charset": "", "filename": "Lu et al. - 2017 - Chondrogenic Differentiation of Mesenchymal Stem C.pdf", "md5": "ac9bc266f9881c144cd527edfb7f6f62", "mtime": 1600458825512, "tags": [], "relations": {}, "dateAdded": "2020-09-18T19:53:45Z", "dateModified": "2020-09-18T19:53:45Z" } }, { "key": "4HT8SXUM", "version": 163, "library": { "type": "group", "id": 316688, "name": "Regenerative/PRP", "links": { "alternate": { "href": "https://www.zotero.org/groups/regenerativeprp", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/316688/items/4HT8SXUM", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/regenerativeprp/items/4HT8SXUM", "type": "text/html" }, "up": { "href": "https://api.zotero.org/groups/316688/items/BNNTJ98D", "type": "application/json" } }, "meta": { "createdByUser": { "id": 472710, "username": "Lancelgoetz", "name": "Lance L. Goetz, M.D.", "links": { "alternate": { "href": "https://www.zotero.org/lancelgoetz", "type": "text/html" } } } }, "data": { "key": "4HT8SXUM", "version": 163, "parentItem": "BNNTJ98D", "itemType": "attachment", "linkMode": "linked_url", "title": "PubMed entry", "accessDate": "2020-09-18T19:53:44Z", "url": "http://www.ncbi.nlm.nih.gov/pubmed/27737727", "note": "", "contentType": "text/html", "charset": "", "tags": [], "relations": {}, "dateAdded": "2020-09-18T19:53:44Z", "dateModified": "2020-09-18T19:53:44Z" } }, { "key": "BNNTJ98D", "version": 163, "library": { "type": "group", "id": 316688, "name": "Regenerative/PRP", "links": { "alternate": { "href": "https://www.zotero.org/groups/regenerativeprp", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/316688/items/BNNTJ98D", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/regenerativeprp/items/BNNTJ98D", "type": "text/html" } }, "meta": { "createdByUser": { "id": 472710, "username": "Lancelgoetz", "name": "Lance L. Goetz, M.D.", "links": { "alternate": { "href": "https://www.zotero.org/lancelgoetz", "type": "text/html" } } }, "creatorSummary": "Lu et al.", "parsedDate": "2017", "numChildren": 2 }, "data": { "key": "BNNTJ98D", "version": 163, "itemType": "journalArticle", "title": "Chondrogenic Differentiation of Mesenchymal Stem Cells in Three-Dimensional Chitosan Film Culture", "creators": [ { "creatorType": "author", "firstName": "Tsai-Jung", "lastName": "Lu" }, { "creatorType": "author", "firstName": "Fang-Yao", "lastName": "Chiu" }, { "creatorType": "author", "firstName": "Hsiao-Ying", "lastName": "Chiu" }, { "creatorType": "author", "firstName": "Ming-Chau", "lastName": "Chang" }, { "creatorType": "author", "firstName": "Shih-Chieh", "lastName": "Hung" } ], "abstractNote": "Articular cartilage has a very limited capacity for self-repair, and mesenchymal stem cells (MSCs) have the potential to treat cartilage defects and osteoarthritis. However, in-depth mechanistic studies regarding their applications are required. Here we demonstrated the use of chitosan film culture for promoting chondrogenic differentiation of MSCs. We found that MSCs formed spheres 2 days after seeding on dishes coated with chitosan. When MSCs were induced in a chondrogenic induction medium on chitosan films, the size of the spheres continuously increased for up to 21 days. Alcian blue staining and immunohistochemistry demonstrated the expression of chondrogenic proteins, including aggrecan, type II collagen, and type X collagen at 14 and 21 days of differentiation. Importantly, chitosan, with a medium molecular weight (size: 190-310 kDa), was more suitable than other sizes for inducing chondrogenic differentiation of MSCs in terms of sphere size and expression of chondrogenic proteins and endochondral markers. We identified that the mechanistic target of rapamycin (mTOR) signaling and its downstream S6 kinase (S6K)/S6 were activated in chitosan film culture compared to that of monolayer culture. The activation of mTOR/S6K was continuously upregulated from days 2 to 7 of differentiation. Furthermore, we found that mTOR/S6K signaling was required for chondrogenic differentiation of MSCs in chitosan film culture through rapamycin treatment and mTOR knockdown. In conclusion, we showed the suitability of chitosan film culture for promoting chondrogenic differentiation of MSCs and its potential in the development of new strategies in cartilage tissue engineering.", "publicationTitle": "Cell Transplantation", "publisher": "", "place": "", "date": "03 13, 2017", "volume": "26", "issue": "3", "section": "", "partNumber": "", "partTitle": "", "pages": "417-427", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "Cell Transplant", "DOI": "10.3727/096368916X693464", "citationKey": "", "url": "", "accessDate": "", "PMID": "", "PMCID": "", "ISSN": "1555-3892", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "eng", "libraryCatalog": "PubMed", "callNumber": "", "rights": "", "extra": "PMID: 27737727\nPMCID: PMC5657699", "tags": [ { "tag": "Aggrecans", "type": 1 }, { "tag": "Blotting, Western", "type": 1 }, { "tag": "Cell Culture Techniques", "type": 1 }, { "tag": "Cell Differentiation", "type": 1 }, { "tag": "Cells, Cultured", "type": 1 }, { "tag": "Chitosan", "type": 1 }, { "tag": "Chondrogenesis", "type": 1 }, { "tag": "Collagen Type II", "type": 1 }, { "tag": "Collagen Type X", "type": 1 }, { "tag": "Humans", "type": 1 }, { "tag": "Immunohistochemistry", "type": 1 }, { "tag": "Mesenchymal Stem Cells", "type": 1 }, { "tag": "Reverse Transcriptase Polymerase Chain Reaction", "type": 1 }, { "tag": "Sirolimus", "type": 1 }, { "tag": "TOR Serine-Threonine Kinases", "type": 1 } ], "collections": [], "relations": {}, "dateAdded": "2020-09-18T19:53:42Z", "dateModified": "2020-09-18T19:53:42Z" } }, { "key": "H5RHHBER", "version": 161, "library": { "type": "group", "id": 316688, "name": "Regenerative/PRP", "links": { "alternate": { "href": "https://www.zotero.org/groups/regenerativeprp", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/316688/items/H5RHHBER", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/regenerativeprp/items/H5RHHBER", "type": "text/html" }, "up": { "href": "https://api.zotero.org/groups/316688/items/CRSKBJVC", "type": "application/json" } }, "meta": { "createdByUser": { "id": 472710, "username": "Lancelgoetz", "name": "Lance L. 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Goetz, M.D.", "links": { "alternate": { "href": "https://www.zotero.org/lancelgoetz", "type": "text/html" } } }, "creatorSummary": "Thorson et al.", "parsedDate": "2019-12", "numChildren": 2 }, "data": { "key": "CRSKBJVC", "version": 160, "itemType": "journalArticle", "title": "Matrix Metalloproteinases and Their Inhibitors and Proteoglycan 4 in Patients Undergoing Total Joint Arthroplasty", "creators": [ { "creatorType": "author", "firstName": "Chase", "lastName": "Thorson" }, { "creatorType": "author", "firstName": "Kevin", "lastName": "Galicia" }, { "creatorType": "author", "firstName": "Andrew", "lastName": "Burleson" }, { "creatorType": "author", "firstName": "Olivia", "lastName": "Bouchard" }, { "creatorType": "author", "firstName": "Debra", "lastName": "Hoppensteadt" }, { "creatorType": "author", "firstName": "Jawed", "lastName": "Fareed" }, { "creatorType": "author", "firstName": "William", "lastName": "Hopkinson" } ], "abstractNote": "Osteoarthritis, a degenerative disease of the joints, is the most common form of arthritis in the knee. Total joint arthoplasty is a commonly used treatment for joint degeneration and osteoarthritis, and due to these factors, TJA for hip and knee joints is projected to grow by 137% and 601% between 2005 and 2030. Matrix metalloproteases are enzymes found in the extracellular matrix that cleave matrix components. Normally MMPs are downregulated in tissues by Tissue Inhibitors of Metalloproteases, or TIMPs. The relative concentration of TIMPs also may denote some of the activity of the MMPs found in serum. Lubricin (proteoglycan 4) is a molecule found in the synovial fluid that protects joints by dissipating strain energy during locomotion. Lubricin synovial fluid concentration is also diminished in many patients with osteoarthritis, but not all. Given the importance of these three sets of molecules, our lab investigated the correlation between circulating lubricin, MMP levels and TIMPs levels. Blood plasma samples were obtained from de-identified subjects undergoing total joint arthroplasty at Loyola University Medical Center and the University of Utah. Normal blood plasma from pooled healthy individuals served as a control. We analyzed biomarker levels in plasma using ELISA. Our data show that MMP-1 and 9 were increased in TJA patients compared to normal controls, while MMP-2 and 13 were decreased. We also found decreased lubricin and tissue factor in surgical patients relative to controls. These data support the idea that lubricin is vital in protecting the synovial joint and that MMPs play a complex role in the destruction of the joint.", "publicationTitle": "Clinical and Applied Thrombosis/Hemostasis: Official Journal of the International Academy of Clinical and Applied Thrombosis/Hemostasis", "publisher": "", "place": "", "date": "2019 Jan-Dec", "volume": "25", "issue": "", "section": "", "partNumber": "", "partTitle": "", "pages": "1076029619828113", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "Clin. Appl. Thromb. Hemost.", "DOI": "10.1177/1076029619828113", "citationKey": "", "url": "", "accessDate": "", "PMID": "", "PMCID": "", "ISSN": "1938-2723", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "eng", "libraryCatalog": "PubMed", "callNumber": "", "rights": "", "extra": "PMID: 30754994\nPMCID: PMC6714937", "tags": [ { "tag": "Adult", "type": 1 }, { "tag": "Aged", "type": 1 }, { "tag": "Aged, 80 and over", "type": 1 }, { "tag": "Arthroplasty", "type": 1 }, { "tag": "Female", "type": 1 }, { "tag": "Humans", "type": 1 }, { "tag": "MMP-1", "type": 1 }, { "tag": "MMP-13", "type": 1 }, { "tag": "MMP-2", "type": 1 }, { "tag": "MMP-9", "type": 1 }, { "tag": "Male", "type": 1 }, { "tag": "Matrix Metalloproteinases", "type": 1 }, { "tag": "Middle Aged", "type": 1 }, { "tag": "Osteoarthritis", "type": 1 }, { "tag": "Proteoglycans", "type": 1 }, { "tag": "arthroplasty", "type": 1 }, { "tag": "lubricin", "type": 1 }, { "tag": "matrix metalloproteinases", "type": 1 }, { "tag": "osteoarthritis", "type": 1 }, { "tag": "tissue inhibitors of matrix metalloproteinases", "type": 1 }, { "tag": "vEGF", "type": 1 } ], "collections": [], "relations": {}, "dateAdded": "2020-04-29T00:56:16Z", "dateModified": "2020-04-29T00:56:16Z" } }, { "key": "5GQ7EAPN", "version": 158, "library": { "type": "group", "id": 316688, "name": "Regenerative/PRP", "links": { "alternate": { "href": "https://www.zotero.org/groups/regenerativeprp", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/316688/items/5GQ7EAPN", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/regenerativeprp/items/5GQ7EAPN", "type": "text/html" }, "up": { "href": "https://api.zotero.org/groups/316688/items/88XEVTY6", "type": "application/json" } }, "meta": { "createdByUser": { "id": 472710, "username": "Lancelgoetz", "name": "Lance L. 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Goetz, M.D.", "links": { "alternate": { "href": "https://www.zotero.org/lancelgoetz", "type": "text/html" } } }, "creatorSummary": "Meszaros and Malemud", "parsedDate": "2012-09", "numChildren": 2 }, "data": { "key": "88XEVTY6", "version": 157, "itemType": "journalArticle", "title": "Prospects for treating osteoarthritis: enzyme-protein interactions regulating matrix metalloproteinase activity", "creators": [ { "creatorType": "author", "firstName": "Evan", "lastName": "Meszaros" }, { "creatorType": "author", "firstName": "Charles J.", "lastName": "Malemud" } ], "abstractNote": "Primary osteoarthritis (OA) is a musculoskeletal disorder of unknown etiology. OA is characterized by an imbalance between anabolism and catabolism in, and altered homeostasis of articular cartilage. Matrix metalloproteinases (MMPs) and a disintegrin and metalloproteinase with thrombospondin motif are upregulated in OA joints. Extracellular matrix (ECM) proteins are critical for resistance to compressive forces and for maintaining the tensile properties of the tissue. Tissue inhibitor of metalloproteinases (TIMPs) is the endogenous inhibitor of MMPs, but in OA, TIMPs do not effectively neutralize MMP activity. Upregulation of MMP gene expression occurs in OA in a milieu of proinflammatory cytokines such as interleukin (IL)-1, IL-6 and tumor necrosis factor α. Presently, the medical therapy of OA includes mainly nonsteroidal anti-inflammatory drugs and corticosteroids which dampen pain and inflammation but appear to have little effect on restoring joint function. Experimental interventions to restore the imbalance between anabolism and catabolism include small molecule inhibitors of MMP subtypes or inhibitors of the interaction between IL-1 and its receptor. Although these agents have some positive effects on reducing MMP subtype activity they have little efficacy at the clinical level. MMP-9 is one MMP subtype implicated in the degradation of articular cartilage ECM proteins. MMP-9 was found in OA synovial fluid as a complex with neutrophil gelatinase-associated lipocalin (NGAL) which protected MMP-9 from autodegradation. Suppressing NGAL synthesis or promoting NGAL degradation may result in reducing the activity of MMP-9. We also propose initiating a search for enzyme-protein interactions to dampen other MMP subtype activity which could suppress ECM protein breakdown.", "publicationTitle": "Therapeutic Advances in Chronic Disease", "publisher": "", "place": "", "date": "Sep 2012", "volume": "3", "issue": "5", "section": "", "partNumber": "", "partTitle": "", "pages": "219-229", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "Ther Adv Chronic Dis", "DOI": "10.1177/2040622312454157", "citationKey": "", "url": "", "accessDate": "", "PMID": "", "PMCID": "", "ISSN": "2040-6223", "archive": "", "archiveLocation": "", "shortTitle": "Prospects for treating osteoarthritis", "language": "eng", "libraryCatalog": "PubMed", "callNumber": "", "rights": "", "extra": "PMID: 23342237\nPMCID: PMC3539270", "tags": [ { "tag": "gelatinase", "type": 1 }, { "tag": "matrix metalloproteinase", "type": 1 }, { "tag": "neutrophil gelatinase-associated lipocalin", "type": 1 }, { "tag": "osteoarthritis", "type": 1 } ], "collections": [], "relations": {}, "dateAdded": "2020-04-29T00:52:56Z", "dateModified": "2020-04-29T00:52:56Z" } }, { "key": "W9DSIWXA", "version": 156, "library": { "type": "group", "id": 316688, "name": "Regenerative/PRP", "links": { "alternate": { "href": "https://www.zotero.org/groups/regenerativeprp", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/316688/items/W9DSIWXA", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/regenerativeprp/items/W9DSIWXA", "type": "text/html" }, "up": { "href": "https://api.zotero.org/groups/316688/items/VPKQZUKY", "type": "application/json" } }, "meta": { "createdByUser": { "id": 472710, "username": "Lancelgoetz", "name": "Lance L. 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