[ { "key": "BUD936B2", "version": 5, "library": { "type": "group", "id": 261320, "name": "aurimas_public", "links": { "alternate": { "href": "https://www.zotero.org/groups/aurimas_public", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/261320/items/BUD936B2", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/aurimas_public/items/BUD936B2", "type": "text/html" }, "up": { "href": "https://api.zotero.org/groups/261320/items/F6QATFMB", "type": "application/json" } }, "meta": { "createdByUser": { "id": 275915, "username": "aurimas", "name": "Aurimas Vinckevicius", "links": { "alternate": { "href": "https://www.zotero.org/aurimas", "type": "text/html" } } } }, "data": { "key": "BUD936B2", "version": 5, "parentItem": "F6QATFMB", "itemType": "attachment", "linkMode": "linked_url", "title": "nprot.2007.249.pdf", "accessDate": "2014-04-21T21:53:12Z", "url": "http://www.nature.com/nprot/journal/v2/n8/pdf/nprot.2007.249.pdf", "note": "", "contentType": "application/pdf", "charset": "", "tags": [], "relations": {}, "dateAdded": "2014-04-21T21:53:12Z", "dateModified": "2014-04-21T21:53:12Z" } }, { "key": "NI7UI6AC", "version": 4, "library": { "type": "group", "id": 261320, "name": "aurimas_public", "links": { "alternate": { "href": "https://www.zotero.org/groups/aurimas_public", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/261320/items/NI7UI6AC", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/aurimas_public/items/NI7UI6AC", "type": "text/html" }, "up": { "href": "https://api.zotero.org/groups/261320/items/9RJCA259", "type": "application/json" } }, "meta": { "createdByUser": { "id": 275915, "username": "aurimas", "name": "Aurimas Vinckevicius", "links": { "alternate": { "href": "https://www.zotero.org/aurimas", "type": "text/html" } } } }, "data": { "key": "NI7UI6AC", "version": 4, "parentItem": "9RJCA259", "itemType": "attachment", "linkMode": "linked_url", "title": "34920.pdf", "accessDate": "2014-04-21T21:50:29Z", "url": "http://cdn.intechopen.com/pdfs/34920.pdf", "note": "", "contentType": "application/pdf", "charset": "", "tags": [], "relations": {}, "dateAdded": "2014-04-21T21:50:29Z", "dateModified": "2014-04-21T21:50:29Z" } }, { "key": "F6QATFMB", "version": 6, "library": { "type": "group", "id": 261320, "name": "aurimas_public", "links": { "alternate": { "href": "https://www.zotero.org/groups/aurimas_public", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/261320/items/F6QATFMB", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/aurimas_public/items/F6QATFMB", "type": "text/html" } }, "meta": { "createdByUser": { "id": 275915, "username": "aurimas", "name": "Aurimas Vinckevicius", "links": { "alternate": { "href": "https://www.zotero.org/aurimas", "type": "text/html" } } }, "creatorSummary": "Hellman and Fried", "parsedDate": "2007-08", "numChildren": 1 }, "data": { "key": "F6QATFMB", "version": 6, "itemType": "journalArticle", "title": "Electrophoretic mobility shift assay (EMSA) for detecting protein-nucleic acid interactions", "creators": [ { "creatorType": "author", "firstName": "Lance M", "lastName": "Hellman" }, { "creatorType": "author", "firstName": "Michael G", "lastName": "Fried" } ], "abstractNote": "The gel electrophoresis mobility shift assay (EMSA) is used to detect protein complexes with nucleic acids. It is the core technology underlying a wide range of qualitative and quantitative analyses for the characterization of interacting systems. In the classical assay, solutions of protein and nucleic acid are combined and the resulting mixtures are subjected to electrophoresis under native conditions through polyacrylamide or agarose gel. After electrophoresis, the distribution of species containing nucleic acid is determined, usually by autoradiography of 32P-labeled nucleic acid. In general, protein–nucleic acid complexes migrate more slowly than the corresponding free nucleic acid. In this protocol, we identify the most important factors that determine the stabilities and electrophoretic mobilities of complexes under assay conditions. A representative protocol is provided and commonly used variants are discussed. Expected outcomes are briefly described. References to extensions of the method and a troubleshooting guide are provided.", "publicationTitle": "Nature Protocols", "publisher": "", "place": "", "date": "August 2007", "volume": "2", "issue": "8", "section": "", "partNumber": "", "partTitle": "", "pages": "1849-1861", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "", "DOI": "10.1038/nprot.2007.249", "citationKey": "", "url": "http://www.nature.com/nprot/journal/v2/n8/full/nprot.2007.249.html", "accessDate": "2012-06-08T20:53:58Z", "PMID": "", "PMCID": "", "ISSN": "1754-2189", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "en", "libraryCatalog": "www.nature.com", "callNumber": "", "rights": "© 2007 Nature Publishing Group", "extra": "", "tags": [], "collections": [ "DCWR2H5X" ], "relations": {}, "dateAdded": "2014-04-21T21:40:57Z", "dateModified": "2014-04-21T21:40:57Z" } }, { "key": "37NSQMVK", "version": 3, "library": { "type": "group", "id": 261320, "name": "aurimas_public", "links": { "alternate": { "href": "https://www.zotero.org/groups/aurimas_public", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/261320/items/37NSQMVK", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/aurimas_public/items/37NSQMVK", "type": "text/html" } }, "meta": { "createdByUser": { "id": 275915, "username": "aurimas", "name": "Aurimas Vinckevicius", "links": { "alternate": { "href": "https://www.zotero.org/aurimas", "type": "text/html" } } }, "creatorSummary": "Kucera and Nichols", "parsedDate": "2001", "numChildren": 0 }, "data": { "key": "37NSQMVK", "version": 3, "itemType": "bookSection", "title": "DNA-Dependent DNA Polymerases", "creators": [ { "creatorType": "author", "firstName": "Rebecca B.", "lastName": "Kucera" }, { "creatorType": "author", "firstName": "Nicole M.", "lastName": "Nichols" } ], "abstractNote": "This unit presents characteristics and reaction conditions of the DNA-dependent DNA polymerases, including E. coli DNA polymerase I and its Klenow fragment, T4 DNA polymerase, native and modified T7 DNA polymerase, phi29 DNA polymerase, Bst DNA polymerase, and Taq DNA polymerase. The unit also provides overviews of other classes of thermophilic DNA polymerases used in PCR applications (described fully in UNIT 15.1), and the rapidly expanding class of lesion-bypass DNA polymerases that play a role in DNA damage repair.", "bookTitle": "Current Protocols in Molecular Biology", "series": "", "seriesNumber": "", "volume": "", "numberOfVolumes": "", "edition": "", "date": "2001", "publisher": "John Wiley & Sons, Inc.", "place": "", "originalDate": "", "originalPublisher": "", "originalPlace": "", "format": "", "pages": "", "ISBN": "9780471142720", "DOI": "", "citationKey": "", "url": "http://onlinelibrary.wiley.com/doi/10.1002/0471142727.mb0305s84/abstract", "accessDate": "2013-04-25T11:05:59Z", "ISSN": "", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "en", "libraryCatalog": "Wiley Online Library", "callNumber": "", "rights": "Copyright © 2008 by John Wiley & Sons, Inc.", "extra": "", "tags": [], "collections": [ "DCWR2H5X" ], "relations": {}, "dateAdded": "2014-04-21T21:40:57Z", "dateModified": "2014-04-21T21:40:57Z" } }, { "key": "9RJCA259", "version": 3, "library": { "type": "group", "id": 261320, "name": "aurimas_public", "links": { "alternate": { "href": "https://www.zotero.org/groups/aurimas_public", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/261320/items/9RJCA259", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/aurimas_public/items/9RJCA259", "type": "text/html" } }, "meta": { "createdByUser": { "id": 275915, "username": "aurimas", "name": "Aurimas Vinckevicius", "links": { "alternate": { "href": "https://www.zotero.org/aurimas", "type": "text/html" } } }, "creatorSummary": "Alves and Cunh", "parsedDate": "2012-04-04", "numChildren": 1 }, "data": { "key": "9RJCA259", "version": 3, "itemType": "bookSection", "title": "Electrophoretic Mobility Shift Assay: Analyzing Protein - Nucleic Acid Interactions", "creators": [ { "creatorType": "editor", "firstName": "Sameh", "lastName": "Magdeldin" }, { "creatorType": "author", "firstName": "Carolina", "lastName": "Alves" }, { "creatorType": "author", "firstName": "Celso", "lastName": "Cunh" } ], "abstractNote": "", "bookTitle": "Gel Electrophoresis - Advanced Techniques", "series": "", "seriesNumber": "", "volume": "", "numberOfVolumes": "", "edition": "", "date": "2012-04-04", "publisher": "InTech", "place": "", "originalDate": "", "originalPublisher": "", "originalPlace": "", "format": "", "pages": "", "ISBN": "978-953-51-0457-5", "DOI": "", "citationKey": "", "url": "http://www.intechopen.com/books/gel-electrophoresis-advanced-techniques/electrophoretic-mobility-shift-assay-analyzing-protein-nucleic-acid-interactions", "accessDate": "2013-10-09T01:21:08Z", "ISSN": "", "archive": "", "archiveLocation": "", "shortTitle": "Electrophoretic Mobility Shift Assay", "language": "", "libraryCatalog": "CrossRef", "callNumber": "", "rights": "", "extra": "", "tags": [], "collections": [ "DCWR2H5X" ], "relations": {}, "dateAdded": "2014-04-21T21:40:57Z", "dateModified": "2014-04-21T21:40:57Z" } }, { "key": "43I4G8S2", "version": 3, "library": { "type": "group", "id": 261320, "name": "aurimas_public", "links": { "alternate": { "href": "https://www.zotero.org/groups/aurimas_public", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/261320/items/43I4G8S2", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/aurimas_public/items/43I4G8S2", "type": "text/html" } }, "meta": { "createdByUser": { "id": 275915, "username": "aurimas", "name": "Aurimas Vinckevicius", "links": { "alternate": { "href": "https://www.zotero.org/aurimas", "type": "text/html" } } }, "creatorSummary": "Buratowski and Chodosh", "parsedDate": "2001", "numChildren": 0 }, "data": { "key": "43I4G8S2", "version": 3, "itemType": "bookSection", "title": "Mobility Shift DNA-Binding Assay Using Gel Electrophoresis", "creators": [ { "creatorType": "author", "firstName": "Stephen", "lastName": "Buratowski" }, { "creatorType": "author", "firstName": "Lewis A.", "lastName": "Chodosh" } ], "abstractNote": "The DNA-binding assay using nondenaturing polyacrylamide gel electrophoresis (PAGE) provides a simple, rapid, and extremely sensitive method for detecting sequence-specific DNA-binding proteins. Proteins that bind specifically to an end-labeled DNA fragment retard the mobility of the fragment during electrophoresis, resulting in discrete bands corresponding to the individual protein-DNA complexes. The assay described in this unit can be used to test binding of purified proteins or of uncharacterized factors found in crude extracts. This assay also permits quantitative determination of the affinity, abundance, association rate constants, dissociation rate constants, and binding specificity of DNA-binding proteins. Three additional protocols describe a competition assay using unlabeled competitor DNA, an antibody supershift assay, and multicomponent gel shift assays.", "bookTitle": "Current Protocols in Molecular Biology", "series": "", "seriesNumber": "", "volume": "", "numberOfVolumes": "", "edition": "", "date": "2001", "publisher": "John Wiley & Sons, Inc.", "place": "", "originalDate": "", "originalPublisher": "", "originalPlace": "", "format": "", "pages": "", "ISBN": "9780471142720", "DOI": "", "citationKey": "", "url": "http://onlinelibrary.wiley.com/doi/10.1002/0471142727.mb1202s36/abstract", "accessDate": "2013-01-30T17:36:19Z", "ISSN": "", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "en", "libraryCatalog": "Wiley Online Library", "callNumber": "", "rights": "Copyright © 2003 by John Wiley and Sons, Inc.", "extra": "", "tags": [], "collections": [ "DCWR2H5X" ], "relations": {}, "dateAdded": "2014-04-21T21:40:57Z", "dateModified": "2014-04-21T21:40:57Z" } } ]