[ { "key": "TXZJDF5K", "version": 7, "library": { "type": "group", "id": 252653, "name": "Lewin Group", "links": { "alternate": { "href": "https://www.zotero.org/groups/lewin_group", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/252653/items/TXZJDF5K", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/lewin_group/items/TXZJDF5K", "type": "text/html" }, "up": { "href": "https://api.zotero.org/groups/252653/items/INNSZ9DB", "type": "application/json" } }, "meta": { "createdByUser": { "id": 283300, "username": "glewin", "name": "Gary Lewin", "links": { "alternate": { "href": "https://www.zotero.org/glewin", "type": "text/html" } } } }, "data": { "key": "TXZJDF5K", "version": 7, "parentItem": "INNSZ9DB", "itemType": "attachment", "linkMode": "linked_url", "title": "PubMed entry", "accessDate": "2014-03-06T20:21:54Z", "url": "http://www.ncbi.nlm.nih.gov/pubmed/24336289", "note": "", "contentType": "text/html", "charset": "", "tags": [], "relations": {}, "dateAdded": "2014-03-06T20:21:54Z", "dateModified": "2014-03-06T20:21:54Z" } }, { "key": "INNSZ9DB", "version": 7, "library": { "type": "group", "id": 252653, "name": "Lewin Group", "links": { "alternate": { "href": "https://www.zotero.org/groups/lewin_group", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/252653/items/INNSZ9DB", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/lewin_group/items/INNSZ9DB", "type": "text/html" } }, "meta": { "createdByUser": { "id": 283300, "username": "glewin", "name": "Gary Lewin", "links": { "alternate": { "href": "https://www.zotero.org/glewin", "type": "text/html" } } }, "creatorSummary": "DeCaen et al.", "parsedDate": "2013-12-12", "numChildren": 1 }, "data": { "key": "INNSZ9DB", "version": 7, "itemType": "journalArticle", "title": "Direct recording and molecular identification of the calcium channel of primary cilia", "creators": [ { "creatorType": "author", "firstName": "Paul G", "lastName": "DeCaen" }, { "creatorType": "author", "firstName": "Markus", "lastName": "Delling" }, { "creatorType": "author", "firstName": "Thuy N", "lastName": "Vien" }, { "creatorType": "author", "firstName": "David E", "lastName": "Clapham" } ], "abstractNote": "A primary cilium is a solitary, slender, non-motile protuberance of structured microtubules (9+0) enclosed by plasma membrane. Housing components of the cell division apparatus between cell divisions, primary cilia also serve as specialized compartments for calcium signalling and hedgehog signalling pathways. Specialized sensory cilia such as retinal photoreceptors and olfactory cilia use diverse ion channels. An ion current has been measured from primary cilia of kidney cells, but the responsible genes have not been identified. The polycystin proteins (PC and PKD), identified in linkage studies of polycystic kidney disease, are candidate channels divided into two structural classes: 11-transmembrane proteins (PKD1, PKD1L1 and PKD1L2) remarkable for a large extracellular amino terminus of putative cell adhesion domains and a G-protein-coupled receptor proteolytic site, and the 6-transmembrane channel proteins (PKD2, PKD2L1 and PKD2L2; TRPPs). Evidence indicates that the PKD1 proteins associate with the PKD2 proteins via coiled-coil domains. Here we use a transgenic mouse in which only cilia express a fluorophore and use it to record directly from primary cilia, and demonstrate that PKD1L1 and PKD2L1 form ion channels at high densities in several cell types. In conjunction with an accompanying manuscript, we show that the PKD1L1-PKD2L1 heteromeric channel establishes the cilia as a unique calcium compartment within cells that modulates established hedgehog pathways.", "publicationTitle": "Nature", "publisher": "", "place": "", "date": "Dec 12, 2013", "volume": "504", "issue": "7479", "section": "", "partNumber": "", "partTitle": "", "pages": "315-318", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "Nature", "DOI": "10.1038/nature12832", "citationKey": "", "url": "", "accessDate": "", "PMID": "", "PMCID": "", "ISSN": "1476-4687", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "eng", "libraryCatalog": "NCBI PubMed", "callNumber": "", "rights": "", "extra": "PMID: 24336289", "tags": [ { "tag": "Animals", "type": 1 }, { "tag": "Calcium Channels", "type": 1 }, { "tag": "Cell Division", "type": 1 }, { "tag": "Cell Line", "type": 1 }, { "tag": "Cell Membrane", "type": 1 }, { "tag": "Cells, Cultured", "type": 1 }, { "tag": "Cilia", "type": 1 }, { "tag": "HEK293 Cells", "type": 1 }, { "tag": "Hedgehog Proteins", "type": 1 }, { "tag": "Humans", "type": 1 }, { "tag": "Membrane Proteins", "type": 1 }, { "tag": "Mice", "type": 1 }, { "tag": "Mice, Transgenic", "type": 1 }, { "tag": "Oncogene Proteins", "type": 1 }, { "tag": "Receptors, Cell Surface", "type": 1 }, { "tag": "Receptors, G-Protein-Coupled", "type": 1 }, { "tag": "Trans-Activators", "type": 1 } ], "collections": [ "FSZKANT5" ], "relations": {}, "dateAdded": "2014-03-06T20:21:54Z", "dateModified": "2014-03-06T20:21:54Z" } }, { "key": "2B3E2C8S", "version": 5, "library": { "type": "group", "id": 252653, "name": "Lewin Group", "links": { "alternate": { "href": "https://www.zotero.org/groups/lewin_group", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/252653/items/2B3E2C8S", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/lewin_group/items/2B3E2C8S", "type": "text/html" }, "up": { "href": "https://api.zotero.org/groups/252653/items/VTR29RZK", "type": "application/json" } }, "meta": { "createdByUser": { "id": 283300, "username": "glewin", "name": "Gary Lewin", "links": { "alternate": { "href": "https://www.zotero.org/glewin", "type": "text/html" } } } }, "data": { "key": "2B3E2C8S", "version": 5, "parentItem": "VTR29RZK", "itemType": "attachment", "linkMode": "linked_url", "title": "PubMed entry", "accessDate": "2014-03-06T20:21:27Z", "url": "http://www.ncbi.nlm.nih.gov/pubmed/24157948", "note": "", "contentType": "text/html", "charset": "", "tags": [], "relations": {}, "dateAdded": "2014-03-06T20:21:27Z", "dateModified": "2014-03-06T20:21:27Z" } }, { "key": "VTR29RZK", "version": 5, "library": { "type": "group", "id": 252653, "name": "Lewin Group", "links": { "alternate": { "href": "https://www.zotero.org/groups/lewin_group", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/252653/items/VTR29RZK", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/lewin_group/items/VTR29RZK", "type": "text/html" } }, "meta": { "createdByUser": { "id": 283300, "username": "glewin", "name": "Gary Lewin", "links": { "alternate": { "href": "https://www.zotero.org/glewin", "type": "text/html" } } }, "creatorSummary": "Peyronnet et al.", "parsedDate": "2013-12", "numChildren": 1 }, "data": { "key": "VTR29RZK", "version": 5, "itemType": "journalArticle", "title": "Piezo1-dependent stretch-activated channels are inhibited by Polycystin-2 in renal tubular epithelial cells", "creators": [ { "creatorType": "author", "firstName": "Rémi", "lastName": "Peyronnet" }, { "creatorType": "author", "firstName": "Joana R", "lastName": "Martins" }, { "creatorType": "author", "firstName": "Fabrice", "lastName": "Duprat" }, { "creatorType": "author", "firstName": "Sophie", "lastName": "Demolombe" }, { "creatorType": "author", "firstName": "Malika", "lastName": "Arhatte" }, { "creatorType": "author", "firstName": "Martine", "lastName": "Jodar" }, { "creatorType": "author", "firstName": "Michel", "lastName": "Tauc" }, { "creatorType": "author", "firstName": "Christophe", "lastName": "Duranton" }, { "creatorType": "author", "firstName": "Marc", "lastName": "Paulais" }, { "creatorType": "author", "firstName": "Jacques", "lastName": "Teulon" }, { "creatorType": "author", "firstName": "Eric", "lastName": "Honoré" }, { "creatorType": "author", "firstName": "Amanda", "lastName": "Patel" } ], "abstractNote": "Mechanical forces associated with fluid flow and/or circumferential stretch are sensed by renal epithelial cells and contribute to both adaptive or disease states. Non-selective stretch-activated ion channels (SACs), characterized by a lack of inactivation and a remarkably slow deactivation, are active at the basolateral side of renal proximal convoluted tubules. Knockdown of Piezo1 strongly reduces SAC activity in proximal convoluted tubule epithelial cells. Similarly, overexpression of Polycystin-2 (PC2) or, to a greater extent its pathogenic mutant PC2-740X, impairs native SACs. Moreover, PC2 inhibits exogenous Piezo1 SAC activity. PC2 coimmunoprecipitates with Piezo1 and deletion of its N-terminal domain prevents both this interaction and inhibition of SAC activity. These findings indicate that renal SACs depend on Piezo1, but are critically conditioned by PC2.", "publicationTitle": "EMBO reports", "publisher": "", "place": "", "date": "Dec 2013", "volume": "14", "issue": "12", "section": "", "partNumber": "", "partTitle": "", "pages": "1143-1148", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "EMBO Rep.", "DOI": "10.1038/embor.2013.170", "citationKey": "", "url": "", "accessDate": "", "PMID": "", "PMCID": "", "ISSN": "1469-3178", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "eng", "libraryCatalog": "NCBI PubMed", "callNumber": "", "rights": "", "extra": "PMID: 24157948", "tags": [], "collections": [ "FSZKANT5" ], "relations": {}, "dateAdded": "2014-03-06T20:21:27Z", "dateModified": "2014-03-06T20:21:27Z" } }, { "key": "742NXP3M", "version": 8, "library": { "type": "group", "id": 252653, "name": "Lewin Group", "links": { "alternate": { "href": "https://www.zotero.org/groups/lewin_group", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/252653/items/742NXP3M", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/lewin_group/items/742NXP3M", "type": "text/html" }, "up": { "href": "https://api.zotero.org/groups/252653/items/TECG66V8", "type": "application/json" } }, "meta": { "createdByUser": { "id": 283300, "username": "glewin", "name": "Gary Lewin", "links": { "alternate": { "href": "https://www.zotero.org/glewin", "type": "text/html" } } }, "numChildren": 0 }, "data": { "key": "742NXP3M", "version": 8, "parentItem": "TECG66V8", "itemType": "attachment", "linkMode": "imported_url", "title": "Full Text PDF", "accessDate": "2014-03-06T20:20:44Z", "url": "http://circres.ahajournals.org/content/98/2/245.full.pdf", "note": "", "contentType": "application/pdf", "charset": "", "filename": "Oancea et al. - 2006 - Functional TRPM7 Channels Accumulate at the Plasma.pdf", "md5": "4a9d396ad5937db2984031ac3ffae02c", "mtime": 1394137278236, "tags": [], "relations": {}, "dateAdded": "2014-03-06T20:20:44Z", "dateModified": "2014-03-06T20:21:18Z" } }, { "key": "JGWUH756", "version": 9, "library": { "type": "group", "id": 252653, "name": "Lewin Group", "links": { "alternate": { "href": "https://www.zotero.org/groups/lewin_group", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/252653/items/JGWUH756", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/lewin_group/items/JGWUH756", "type": "text/html" }, "up": { "href": "https://api.zotero.org/groups/252653/items/TECG66V8", "type": "application/json" } }, "meta": { "createdByUser": { "id": 283300, "username": "glewin", "name": "Gary Lewin", "links": { "alternate": { "href": "https://www.zotero.org/glewin", "type": "text/html" } } } }, "data": { "key": "JGWUH756", "version": 9, "parentItem": "TECG66V8", "itemType": "attachment", "linkMode": "imported_url", "title": "Snapshot", "accessDate": "2014-03-06T20:20:44Z", "url": "http://circres.ahajournals.org/content/98/2/245.long", "note": "", "contentType": "text/html", "charset": "utf-8", "filename": "245.html", "md5": "8c2b8e991d5c2c10a52684b5a547467c", "mtime": 1394137246000, "tags": [], "relations": {}, "dateAdded": "2014-03-06T20:20:44Z", "dateModified": "2014-03-06T20:20:44Z" } }, { "key": "CWRA8J9X", "version": 4, "library": { "type": "group", "id": 252653, "name": "Lewin Group", "links": { "alternate": { "href": "https://www.zotero.org/groups/lewin_group", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/252653/items/CWRA8J9X", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/lewin_group/items/CWRA8J9X", "type": "text/html" }, "up": { "href": "https://api.zotero.org/groups/252653/items/TECG66V8", "type": "application/json" } }, "meta": { "createdByUser": { "id": 283300, "username": "glewin", "name": "Gary Lewin", "links": { "alternate": { "href": "https://www.zotero.org/glewin", "type": "text/html" } } } }, "data": { "key": "CWRA8J9X", "version": 4, "parentItem": "TECG66V8", "itemType": "attachment", "linkMode": "linked_url", "title": "PubMed entry", "accessDate": "2014-03-06T20:20:44Z", "url": "http://www.ncbi.nlm.nih.gov/pubmed/16357306", "note": "", "contentType": "text/html", "charset": "", "tags": [], "relations": {}, "dateAdded": "2014-03-06T20:20:44Z", "dateModified": "2014-03-06T20:20:44Z" } }, { "key": "TECG66V8", "version": 4, "library": { "type": "group", "id": 252653, "name": "Lewin Group", "links": { "alternate": { "href": "https://www.zotero.org/groups/lewin_group", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/252653/items/TECG66V8", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/lewin_group/items/TECG66V8", "type": "text/html" } }, "meta": { "createdByUser": { "id": 283300, "username": "glewin", "name": "Gary Lewin", "links": { "alternate": { "href": "https://www.zotero.org/glewin", "type": "text/html" } } }, "creatorSummary": "Oancea et al.", "parsedDate": "2006-02-03", "numChildren": 3 }, "data": { "key": "TECG66V8", "version": 4, "itemType": "journalArticle", "title": "Functional TRPM7 Channels Accumulate at the Plasma Membrane in Response to Fluid Flow", "creators": [ { "creatorType": "author", "firstName": "Elena", "lastName": "Oancea" }, { "creatorType": "author", "firstName": "Joshua T.", "lastName": "Wolfe" }, { "creatorType": "author", "firstName": "David E.", "lastName": "Clapham" } ], "abstractNote": "Many cells are constantly exposed to fluid mechanical forces generated by flowing blood, and wall shear stresses modulate aspects of their structure and function. However, the mechanisms for mechanotransduction of flow are not well understood. Here we report that TRPM7, which is both an ion channel and a functional kinase, is translocated within cells in response to laminar flow. After exposure of cells to physiological values of laminar fluid flow, the number of TRPM7 molecules localized at or near the plasma membrane increased up to 2-fold, in less than 100 seconds. This increase in membrane-localized GFP-TRPM7, as seen by total internal reflection fluorescence microscopy, closely correlated with increases in TRPM7 current. Both endogenous and heterologously expressed TRPM7 was found in tubulovesicular structures that were translocated to the region of the plasma membrane on induction of shear stress. In vascular smooth muscle cells, but not in several types of endothelial cells, fluid flow increased endogenous native TRPM7 current amplitude. We hypothesize that TRPM7 plays a role in pathological response to vessel wall injury.", "publicationTitle": "Circulation Research", "publisher": "", "place": "", "date": "02/03/2006", "volume": "98", "issue": "2", "section": "", "partNumber": "", "partTitle": "", "pages": "245-253", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "Circulation Research", "DOI": "10.1161/01.RES.0000200179.29375.cc", "citationKey": "", "url": "http://circres.ahajournals.org/content/98/2/245", "accessDate": "2014-03-06T20:20:44Z", "PMID": "", "PMCID": "", "ISSN": "0009-7330, 1524-4571", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "en", "libraryCatalog": "circres.ahajournals.org", "callNumber": "", "rights": "", "extra": "PMID: 16357306", "tags": [ { "tag": "TRP ion channels", "type": 1 }, { "tag": "TRPM7", "type": 1 }, { "tag": "shear force", "type": 1 }, { "tag": "total internal reflection fluorescence (TIRF) microscopy", "type": 1 }, { "tag": "vascular smooth muscle cells", "type": 1 } ], "collections": [ "FSZKANT5" ], "relations": {}, "dateAdded": "2014-03-06T20:20:44Z", "dateModified": "2014-03-06T20:20:44Z" } }, { "key": "AIWUVBP5", "version": 6, "library": { "type": "group", "id": 252653, "name": "Lewin Group", "links": { "alternate": { "href": "https://www.zotero.org/groups/lewin_group", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/252653/items/AIWUVBP5", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/lewin_group/items/AIWUVBP5", "type": "text/html" }, "up": { "href": "https://api.zotero.org/groups/252653/items/QHSFNNW9", "type": "application/json" } }, "meta": { "createdByUser": { "id": 283300, "username": "glewin", "name": "Gary Lewin", "links": { "alternate": { "href": "https://www.zotero.org/glewin", "type": "text/html" } } }, "numChildren": 0 }, "data": { "key": "AIWUVBP5", "version": 6, "parentItem": "QHSFNNW9", "itemType": "attachment", "linkMode": "imported_url", "title": "Full Text PDF", "accessDate": "2014-03-06T20:20:10Z", "url": "http://ajpcell.physiology.org/content/292/1/C460.full.pdf", "note": "", "contentType": "application/pdf", "charset": "", "filename": "Numata et al. - 2007 - TRPM7 is a stretch- and swelling-activated cation .pdf", "md5": "b4e2428bc96e26866805d1d1bfc93873", "mtime": 1394137222053, "tags": [], "relations": {}, "dateAdded": "2014-03-06T20:20:10Z", "dateModified": "2014-03-06T20:20:22Z" } }, { "key": "4GUUVDUF", "version": 10, "library": { "type": "group", "id": 252653, "name": "Lewin Group", "links": { "alternate": { "href": "https://www.zotero.org/groups/lewin_group", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/252653/items/4GUUVDUF", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/lewin_group/items/4GUUVDUF", "type": "text/html" }, "up": { "href": "https://api.zotero.org/groups/252653/items/QHSFNNW9", "type": "application/json" } }, "meta": { "createdByUser": { "id": 283300, "username": "glewin", "name": "Gary Lewin", "links": { "alternate": { "href": "https://www.zotero.org/glewin", "type": "text/html" } } } }, "data": { "key": "4GUUVDUF", "version": 10, "parentItem": "QHSFNNW9", "itemType": "attachment", "linkMode": "imported_url", "title": "Snapshot", "accessDate": "2014-03-06T20:20:10Z", "url": "http://ajpcell.physiology.org/content/292/1/C460", "note": "", "contentType": "text/html", "charset": "utf-8", "filename": "C460.html", "md5": "dd1189c8deda93dd349473ad050df8a7", "mtime": 1394137212000, "tags": [], "relations": {}, "dateAdded": "2014-03-06T20:20:10Z", "dateModified": "2014-03-06T20:20:10Z" } }, { "key": "HIPXE52T", "version": 3, "library": { "type": "group", "id": 252653, "name": "Lewin Group", "links": { "alternate": { "href": "https://www.zotero.org/groups/lewin_group", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/252653/items/HIPXE52T", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/lewin_group/items/HIPXE52T", "type": "text/html" }, "up": { "href": "https://api.zotero.org/groups/252653/items/QHSFNNW9", "type": "application/json" } }, "meta": { "createdByUser": { "id": 283300, "username": "glewin", "name": "Gary Lewin", "links": { "alternate": { "href": "https://www.zotero.org/glewin", "type": "text/html" } } } }, "data": { "key": "HIPXE52T", "version": 3, "parentItem": "QHSFNNW9", "itemType": "attachment", "linkMode": "linked_url", "title": "PubMed entry", "accessDate": "2014-03-06T20:20:10Z", "url": "http://www.ncbi.nlm.nih.gov/pubmed/16943238", "note": "", "contentType": "text/html", "charset": "", "tags": [], "relations": {}, "dateAdded": "2014-03-06T20:20:10Z", "dateModified": "2014-03-06T20:20:10Z" } }, { "key": "QHSFNNW9", "version": 3, "library": { "type": "group", "id": 252653, "name": "Lewin Group", "links": { "alternate": { "href": "https://www.zotero.org/groups/lewin_group", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/252653/items/QHSFNNW9", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/lewin_group/items/QHSFNNW9", "type": "text/html" } }, "meta": { "createdByUser": { "id": 283300, "username": "glewin", "name": "Gary Lewin", "links": { "alternate": { "href": "https://www.zotero.org/glewin", "type": "text/html" } } }, "creatorSummary": "Numata et al.", "parsedDate": "2007-01-01", "numChildren": 3 }, "data": { "key": "QHSFNNW9", "version": 3, "itemType": "journalArticle", "title": "TRPM7 is a stretch- and swelling-activated cation channel involved in volume regulation in human epithelial cells", "creators": [ { "creatorType": "author", "firstName": "Tomohiro", "lastName": "Numata" }, { "creatorType": "author", "firstName": "Takahiro", "lastName": "Shimizu" }, { "creatorType": "author", "firstName": "Yasunobu", "lastName": "Okada" } ], "abstractNote": "Stretch- and swelling-activated cation (SSAC) channels play essential roles not only in sensing and transducing external mechanical stresses but also in regulating cell volume in living cells. However, the molecular nature of the SSAC channel has not been clarified. In human epithelial HeLa cells, single-channel recordings in cell-attached and inside-out patches revealed expression of a Mg2+- and Gd3+-sensitive nonselective cation channel that is exquisitely sensitive to membrane stretch. Whole cell recordings revealed that the macroscopic cationic currents exhibit transient receptor potential (TRP) melastatin (TRPM)7-like properties such as outward rectification and sensitivity to Mg2+ and Gd3+. The whole cell cation current was augmented by osmotic cell swelling. RT-PCR and Western blotting demonstrated molecular expression of TRPM7 in HeLa cells. Treatment with small interfering RNA (siRNA) targeted against TRPM7 led to abolition of single stretch-activated cation channel currents and of swelling-activated, whole cell cation currents in HeLa cells. The silencing of TRPM7 by siRNA reduced the rate of cell volume recovery after osmotic swelling. A similar inhibition of regulatory volume decrease was also observed when extracellular Ca2+ was removed or Gd3+ was applied. It is thus concluded that TRPM7 represents the SSAC channel endogenously expressed in HeLa cells and that, by serving as a swelling-induced Ca2+ influx pathway, it plays an important role in cell volume regulation.", "publicationTitle": "American Journal of Physiology - Cell Physiology", "publisher": "", "place": "", "date": "01/01/2007", "volume": "292", "issue": "1", "section": "", "partNumber": "", "partTitle": "", "pages": "C460-C467", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "", "DOI": "10.1152/ajpcell.00367.2006", "citationKey": "", "url": "http://ajpcell.physiology.org/content/292/1/C460", "accessDate": "2014-03-06T20:20:10Z", "PMID": "", "PMCID": "", "ISSN": "", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "en", "libraryCatalog": "ajpcell.physiology.org", "callNumber": "", "rights": "Copyright © 2007 the American Physiological Society", "extra": "Stretch- and swelling-activated cation (SSAC) channels play essential roles not only in sensing and transducing external mechanical stresses but also in regulating cell volume in living cells. However, the molecular nature of the SSAC channel has not been clarified. In human epithelial HeLa cells, single-channel recordings in cell-attached and inside-out patches revealed expression of a Mg2+- and Gd3+-sensitive nonselective cation channel that is exquisitely sensitive to membrane stretch. Whole cell recordings revealed that the macroscopic cationic currents exhibit transient receptor potential (TRP) melastatin (TRPM)7-like properties such as outward rectification and sensitivity to Mg2+ and Gd3+. The whole cell cation current was augmented by osmotic cell swelling. RT-PCR and Western blotting demonstrated molecular expression of TRPM7 in HeLa cells. Treatment with small interfering RNA (siRNA) targeted against TRPM7 led to abolition of single stretch-activated cation channel currents and of swelling-activated, whole cell cation currents in HeLa cells. The silencing of TRPM7 by siRNA reduced the rate of cell volume recovery after osmotic swelling. A similar inhibition of regulatory volume decrease was also observed when extracellular Ca2+ was removed or Gd3+ was applied. It is thus concluded that TRPM7 represents the SSAC channel endogenously expressed in HeLa cells and that, by serving as a swelling-induced Ca2+ influx pathway, it plays an important role in cell volume regulation.\nPMID: 16943238", "tags": [], "collections": [], "relations": {}, "dateAdded": "2014-03-06T20:20:10Z", "dateModified": "2014-03-06T20:20:10Z" } }, { "key": "6GHWNKV9", "version": 5, "library": { "type": "group", "id": 252653, "name": "Lewin Group", "links": { "alternate": { "href": "https://www.zotero.org/groups/lewin_group", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/252653/items/6GHWNKV9", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/lewin_group/items/6GHWNKV9", "type": "text/html" }, "up": { "href": "https://api.zotero.org/groups/252653/items/EX9VVGVZ", "type": "application/json" } }, "meta": { "createdByUser": { "id": 283300, "username": "glewin", "name": "Gary Lewin", "links": { "alternate": { "href": "https://www.zotero.org/glewin", "type": "text/html" } } }, "numChildren": 0 }, "data": { "key": "6GHWNKV9", "version": 5, "parentItem": "EX9VVGVZ", "itemType": "attachment", "linkMode": "imported_url", "title": "Full Text PDF", "accessDate": "2014-03-06T20:17:58Z", "url": "http://ajpcell.physiology.org/content/292/1/C460.full.pdf", "note": "", "contentType": "application/pdf", "charset": "", "filename": "Numata et al. - 2007 - TRPM7 is a stretch- and swelling-activated cation .pdf", "md5": "b4e2428bc96e26866805d1d1bfc93873", "mtime": 1394137094682, "tags": [], "relations": {}, "dateAdded": "2014-03-06T20:17:58Z", "dateModified": "2014-03-06T20:18:14Z" } }, { "key": "R6VZ9UVD", "version": 10, "library": { "type": "group", "id": 252653, "name": "Lewin Group", "links": { "alternate": { "href": "https://www.zotero.org/groups/lewin_group", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/252653/items/R6VZ9UVD", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/lewin_group/items/R6VZ9UVD", "type": "text/html" }, "up": { "href": "https://api.zotero.org/groups/252653/items/EX9VVGVZ", "type": "application/json" } }, "meta": { "createdByUser": { "id": 283300, "username": "glewin", "name": "Gary Lewin", "links": { "alternate": { "href": "https://www.zotero.org/glewin", "type": "text/html" } } } }, "data": { "key": "R6VZ9UVD", "version": 10, "parentItem": "EX9VVGVZ", "itemType": "attachment", "linkMode": "imported_url", "title": "Snapshot", "accessDate": "2014-03-06T20:17:58Z", "url": "http://ajpcell.physiology.org/content/292/1/C460", "note": "", "contentType": "text/html", "charset": "utf-8", "filename": "C460.html", "md5": "d7be84f0e83d07c7c439b19c4c9c75a5", "mtime": 1394137080000, "tags": [], "relations": {}, "dateAdded": "2014-03-06T20:17:58Z", "dateModified": "2014-03-06T20:17:58Z" } }, { "key": "EX9VVGVZ", "version": 2, "library": { "type": "group", "id": 252653, "name": "Lewin Group", "links": { "alternate": { "href": "https://www.zotero.org/groups/lewin_group", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/252653/items/EX9VVGVZ", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/lewin_group/items/EX9VVGVZ", "type": "text/html" } }, "meta": { "createdByUser": { "id": 283300, "username": "glewin", "name": "Gary Lewin", "links": { "alternate": { "href": "https://www.zotero.org/glewin", "type": "text/html" } } }, "creatorSummary": "Numata et al.", "parsedDate": "2007-01-01", "numChildren": 3 }, "data": { "key": "EX9VVGVZ", "version": 2, "itemType": "journalArticle", "title": "TRPM7 is a stretch- and swelling-activated cation channel involved in volume regulation in human epithelial cells", "creators": [ { "creatorType": "author", "firstName": "Tomohiro", "lastName": "Numata" }, { "creatorType": "author", "firstName": "Takahiro", "lastName": "Shimizu" }, { "creatorType": "author", "firstName": "Yasunobu", "lastName": "Okada" } ], "abstractNote": "Stretch- and swelling-activated cation (SSAC) channels play essential roles not only in sensing and transducing external mechanical stresses but also in regulating cell volume in living cells. However, the molecular nature of the SSAC channel has not been clarified. In human epithelial HeLa cells, single-channel recordings in cell-attached and inside-out patches revealed expression of a Mg2+- and Gd3+-sensitive nonselective cation channel that is exquisitely sensitive to membrane stretch. Whole cell recordings revealed that the macroscopic cationic currents exhibit transient receptor potential (TRP) melastatin (TRPM)7-like properties such as outward rectification and sensitivity to Mg2+ and Gd3+. The whole cell cation current was augmented by osmotic cell swelling. RT-PCR and Western blotting demonstrated molecular expression of TRPM7 in HeLa cells. Treatment with small interfering RNA (siRNA) targeted against TRPM7 led to abolition of single stretch-activated cation channel currents and of swelling-activated, whole cell cation currents in HeLa cells. The silencing of TRPM7 by siRNA reduced the rate of cell volume recovery after osmotic swelling. A similar inhibition of regulatory volume decrease was also observed when extracellular Ca2+ was removed or Gd3+ was applied. It is thus concluded that TRPM7 represents the SSAC channel endogenously expressed in HeLa cells and that, by serving as a swelling-induced Ca2+ influx pathway, it plays an important role in cell volume regulation.", "publicationTitle": "American Journal of Physiology - Cell Physiology", "publisher": "", "place": "", "date": "01/01/2007", "volume": "292", "issue": "1", "section": "", "partNumber": "", "partTitle": "", "pages": "C460-C467", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "", "DOI": "10.1152/ajpcell.00367.2006", "citationKey": "", "url": "http://ajpcell.physiology.org/content/292/1/C460", "accessDate": "2014-03-06T20:17:58Z", "PMID": "", "PMCID": "", "ISSN": "", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "en", "libraryCatalog": "ajpcell.physiology.org", "callNumber": "", "rights": "Copyright © 2007 the American Physiological Society", "extra": "Stretch- and swelling-activated cation (SSAC) channels play essential roles not only in sensing and transducing external mechanical stresses but also in regulating cell volume in living cells. However, the molecular nature of the SSAC channel has not been clarified. In human epithelial HeLa cells, single-channel recordings in cell-attached and inside-out patches revealed expression of a Mg2+- and Gd3+-sensitive nonselective cation channel that is exquisitely sensitive to membrane stretch. Whole cell recordings revealed that the macroscopic cationic currents exhibit transient receptor potential (TRP) melastatin (TRPM)7-like properties such as outward rectification and sensitivity to Mg2+ and Gd3+. The whole cell cation current was augmented by osmotic cell swelling. RT-PCR and Western blotting demonstrated molecular expression of TRPM7 in HeLa cells. Treatment with small interfering RNA (siRNA) targeted against TRPM7 led to abolition of single stretch-activated cation channel currents and of swelling-activated, whole cell cation currents in HeLa cells. The silencing of TRPM7 by siRNA reduced the rate of cell volume recovery after osmotic swelling. A similar inhibition of regulatory volume decrease was also observed when extracellular Ca2+ was removed or Gd3+ was applied. It is thus concluded that TRPM7 represents the SSAC channel endogenously expressed in HeLa cells and that, by serving as a swelling-induced Ca2+ influx pathway, it plays an important role in cell volume regulation.\nPMID: 16943238", "tags": [], "collections": [ "FSZKANT5" ], "relations": {}, "dateAdded": "2014-03-06T20:17:58Z", "dateModified": "2014-03-06T20:17:58Z" } }, { "key": "8QJRPQNU", "version": 2, "library": { "type": "group", "id": 252653, "name": "Lewin Group", "links": { "alternate": { "href": "https://www.zotero.org/groups/lewin_group", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/252653/items/8QJRPQNU", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/lewin_group/items/8QJRPQNU", "type": "text/html" }, "up": { "href": "https://api.zotero.org/groups/252653/items/EX9VVGVZ", "type": "application/json" } }, "meta": { "createdByUser": { "id": 283300, "username": "glewin", "name": "Gary Lewin", "links": { "alternate": { "href": "https://www.zotero.org/glewin", "type": "text/html" } } } }, "data": { "key": "8QJRPQNU", "version": 2, "parentItem": "EX9VVGVZ", "itemType": "attachment", "linkMode": "linked_url", "title": "PubMed entry", "accessDate": "2014-03-06T20:17:58Z", "url": "http://www.ncbi.nlm.nih.gov/pubmed/16943238", "note": "", "contentType": "text/html", "charset": "", "tags": [], "relations": {}, "dateAdded": "2014-03-06T20:17:58Z", "dateModified": "2014-03-06T20:17:58Z" } } ]