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"cmorg13", "name": "", "links": { "alternate": { "href": "https://www.zotero.org/cmorg13", "type": "text/html" } } }, "creatorSummary": "Rumbo-Feal et al.", "parsedDate": "2013", "numChildren": 1 }, "data": { "key": "RKEV7CW4", "version": 96, "itemType": "journalArticle", "title": "Whole transcriptome analysis of Acinetobacter baumannii assessed by RNA-sequencing reveals different mRNA expression profiles in biofilm compared to planktonic cells", "creators": [ { "creatorType": "author", "firstName": "Soraya", "lastName": "Rumbo-Feal" }, { "creatorType": "author", "firstName": "Manuel J", "lastName": "Gómez" }, { "creatorType": "author", "firstName": "Carmen", "lastName": "Gayoso" }, { "creatorType": "author", "firstName": "Laura", "lastName": "Álvarez-Fraga" }, { "creatorType": "author", "firstName": "María P", "lastName": "Cabral" }, { "creatorType": "author", "firstName": "Ana M", "lastName": "Aransay" }, { "creatorType": "author", "firstName": "Naiara", "lastName": "Rodríguez-Ezpeleta" }, { "creatorType": "author", "firstName": "Ane", "lastName": "Fullaondo" }, { "creatorType": "author", "firstName": "Jaione", "lastName": "Valle" }, { "creatorType": "author", "firstName": "María", "lastName": "Tomás" }, { "creatorType": "author", "firstName": "Germán", "lastName": "Bou" }, { "creatorType": "author", "firstName": "Margarita", "lastName": "Poza" } ], "abstractNote": "Acinetobacterbaumannii has emerged as a dangerous opportunistic pathogen, with many strains able to form biofilms and thus cause persistent infections. The aim of the present study was to use high-throughput sequencing techniques to establish complete transcriptome profiles of planktonic (free-living) and sessile (biofilm) forms of A. baumannii ATCC 17978 and thereby identify differences in their gene expression patterns. Collections of mRNA from planktonic (both exponential and stationary phase cultures) and sessile (biofilm) cells were sequenced. Six mRNA libraries were prepared following the mRNA-Seq protocols from Illumina. Reads were obtained in a HiScanSQ platform and mapped against the complete genome to describe the complete mRNA transcriptomes of planktonic and sessile cells. The results showed that the gene expression pattern of A. baumannii biofilm cells was distinct from that of planktonic cells, including 1621 genes over-expressed in biofilms relative to stationary phase cells and 55 genes expressed only in biofilms. These differences suggested important changes in amino acid and fatty acid metabolism, motility, active transport, DNA-methylation, iron acquisition, transcriptional regulation, and quorum sensing, among other processes. Disruption or deletion of five of these genes caused a significant decrease in biofilm formation ability in the corresponding mutant strains. Among the genes over-expressed in biofilm cells were those in an operon involved in quorum sensing. One of them, encoding an acyl carrier protein, was shown to be involved in biofilm formation as demonstrated by the significant decrease in biofilm formation by the corresponding knockout strain. The present work serves as a basis for future studies examining the complex network systems that regulate bacterial biofilm formation and maintenance.", "publicationTitle": "PloS one", "publisher": "", "place": "", "date": "2013", "volume": "8", "issue": "8", "section": "", "partNumber": "", "partTitle": "", "pages": "e72968", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "PLoS ONE", "DOI": "10.1371/journal.pone.0072968", "citationKey": "", "url": "", "accessDate": "", "PMID": "", "PMCID": "", "ISSN": "1932-6203", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "eng", "libraryCatalog": "NCBI PubMed", "callNumber": "", "rights": "", "extra": "PMID: 24023660 \nPMCID: PMC3758355", "tags": [], "collections": [], "relations": {}, "dateAdded": "2014-06-02T15:08:35Z", "dateModified": "2014-06-02T15:08:35Z" } }, { "key": "MD6IUMJE", "version": 96, "library": { "type": "group", "id": 252250, "name": "Summer Research Grant", "links": { "alternate": { "href": "https://www.zotero.org/groups/summer_research_grant", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/252250/items/MD6IUMJE", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/summer_research_grant/items/MD6IUMJE", "type": "text/html" } }, "meta": { "createdByUser": { "id": 1822692, "username": "cmorg13", "name": "", "links": { "alternate": { "href": "https://www.zotero.org/cmorg13", "type": "text/html" } } }, "creatorSummary": "Fujisawa et al.", "parsedDate": "2008-11", "numChildren": 1 }, "data": { "key": "MD6IUMJE", "version": 96, "itemType": "journalArticle", "title": "Thermostability of allicin determined by chemical and biological assays", "creators": [ { "creatorType": "author", "firstName": "Hiroyuki", "lastName": "Fujisawa" }, { "creatorType": "author", "firstName": "Kaoru", "lastName": "Suma" }, { "creatorType": "author", "firstName": "Kana", "lastName": "Origuchi" }, { "creatorType": "author", "firstName": "Taiichiro", "lastName": "Seki" }, { "creatorType": "author", "firstName": "Toyohiko", "lastName": "Ariga" } ], "abstractNote": "The garlic-derived antibacterial principle, alk(en)yl sulfinate compounds, has long been considered as very short-lived substance. However, there are some data showing a rather more stable nature of allicin. We determined here the thermostability of allicin by a systematic analyses employing chemical quantification and an antibacterial activity assay. Allicin in an aqueous extract of garlic was degraded stoichiometrically in proportion to the temperature; we estimated the half-life of allicin to be about a year at 4 degrees C (from 1.8 mg/ml to 0.9 mg/ml) and 32 d at 15 degrees C, but only 1 d at 37 degrees C (from 2.0 mg/ml to 1.0 mg/ml). The half-life values for antibacterial activity showed a similar trend in results: 63 d or more at 4 degrees C for both antibacterial activities, 14 d for anti-staphylococcal activity, and 26 d for anti-escherichia activity at 15 degrees C, but only 1.2 d and 1.9 d for the respective activities at 37 degrees C. Such antibacterial activities were attributable to the major allicin, allyl 2-propenylthiosulfinate. Surprisingly, the decline in the quantity of allicin was not accompanied by its degradation; instead, allicin became a larger molecule, ajoene, which was 3-times larger than allicin.", "publicationTitle": "Bioscience, biotechnology, and biochemistry", "publisher": "", "place": "", "date": "Nov 2008", "volume": "72", "issue": "11", "section": "", "partNumber": "", "partTitle": "", "pages": "2877-2883", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "Biosci. Biotechnol. Biochem.", "DOI": "", "citationKey": "", "url": "", "accessDate": "", "PMID": "", "PMCID": "", "ISSN": "1347-6947", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "eng", "libraryCatalog": "NCBI PubMed", "callNumber": "", "rights": "", "extra": "PMID: 18997429", "tags": [ { "tag": "Anti-Bacterial Agents", "type": 1 }, { "tag": "Biological Assay", "type": 1 }, { "tag": "Chromatography, High Pressure Liquid", "type": 1 }, { "tag": "Escherichia coli", "type": 1 }, { "tag": "Garlic", "type": 1 }, { "tag": "Half-Life", "type": 1 }, { "tag": "Plant Extracts", "type": 1 }, { "tag": "Staphylococcus aureus", "type": 1 }, { "tag": "Sulfinic Acids", "type": 1 }, { "tag": "Temperature", "type": 1 }, { "tag": "Water", "type": 1 } ], "collections": [], "relations": {}, "dateAdded": "2014-06-02T15:08:35Z", "dateModified": "2014-06-02T15:08:35Z" } }, { "key": "FEQ7EIHJ", "version": 96, "library": { "type": "group", "id": 252250, "name": "Summer Research Grant", "links": { "alternate": { "href": "https://www.zotero.org/groups/summer_research_grant", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/252250/items/FEQ7EIHJ", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/summer_research_grant/items/FEQ7EIHJ", "type": "text/html" } }, "meta": { "createdByUser": { "id": 1822692, "username": "cmorg13", "name": "", "links": { "alternate": { "href": "https://www.zotero.org/cmorg13", "type": "text/html" } } }, "creatorSummary": "Dötsch et al.", "parsedDate": "2012-02-03", "numChildren": 2 }, "data": { "key": "FEQ7EIHJ", "version": 96, "itemType": "journalArticle", "title": "The Pseudomonas aeruginosa Transcriptome in Planktonic Cultures and Static Biofilms Using RNA Sequencing", "creators": [ { "creatorType": "author", "firstName": "Andreas", "lastName": "Dötsch" }, { "creatorType": "author", "firstName": "Denitsa", "lastName": "Eckweiler" }, { "creatorType": "author", "firstName": "Monika", "lastName": "Schniederjans" }, { "creatorType": "author", "firstName": "Ariane", "lastName": "Zimmermann" }, { "creatorType": "author", "firstName": "Vanessa", "lastName": "Jensen" }, { "creatorType": "author", "firstName": "Maren", "lastName": "Scharfe" }, { "creatorType": "author", "firstName": "Robert", "lastName": "Geffers" }, { "creatorType": "author", "firstName": "Susanne", "lastName": "Häussler" } ], "abstractNote": "In this study, we evaluated how gene expression differs in mature Pseudomonas aeruginosa biofilms as opposed to planktonic cells by the use of RNA sequencing technology that gives rise to both quantitative and qualitative information on the transcriptome. Although a large proportion of genes were consistently regulated in both the stationary phase and biofilm cultures as opposed to the late exponential growth phase cultures, the global biofilm gene expression pattern was clearly distinct indicating that biofilms are not just surface attached cells in stationary phase. A large amount of the genes found to be biofilm specific were involved in adaptation to microaerophilic growth conditions, repression of type three secretion and production of extracellular matrix components. Additionally, we found many small RNAs to be differentially regulated most of them similarly in stationary phase cultures and biofilms. A qualitative analysis of the RNA-seq data revealed more than 3000 putative transcriptional start sites (TSS). By the use of rapid amplification of cDNA ends (5′-RACE) we confirmed the presence of three different TSS associated with the pqsABCDE operon, two in the promoter of pqsA and one upstream of the second gene, pqsB. Taken together, this study reports the first transcriptome study on P. aeruginosa that employs RNA sequencing technology and provides insights into the quantitative and qualitative transcriptome including the expression of small RNAs in P. aeruginosa biofilms.", "publicationTitle": "PLoS ONE", "publisher": "", "place": "", "date": "February 3, 2012", "volume": "7", "issue": "2", "section": "", "partNumber": "", "partTitle": "", "pages": "e31092", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "PLoS ONE", "DOI": "10.1371/journal.pone.0031092", "citationKey": "", "url": "http://dx.doi.org/10.1371/journal.pone.0031092", "accessDate": "2014-03-05T19:46:28Z", "PMID": "", "PMCID": "", "ISSN": "", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "", "libraryCatalog": "PLoS Journals", "callNumber": "", "rights": "", "extra": "", "tags": [], "collections": [], "relations": {}, "dateAdded": "2014-06-02T15:08:35Z", "dateModified": "2014-06-02T15:08:35Z" } }, { "key": "62N52CHX", "version": 96, "library": { "type": "group", "id": 252250, "name": "Summer Research Grant", "links": { "alternate": { "href": "https://www.zotero.org/groups/summer_research_grant", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/252250/items/62N52CHX", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/summer_research_grant/items/62N52CHX", "type": "text/html" } }, "meta": { "createdByUser": { "id": 1822692, "username": "cmorg13", "name": "", "links": { "alternate": { "href": "https://www.zotero.org/cmorg13", "type": "text/html" } } }, "creatorSummary": "Morkunas et al.", "parsedDate": "2012-11-14", "numChildren": 1 }, "data": { "key": "62N52CHX", "version": 96, "itemType": "journalArticle", "title": "Inhibition of the production of the Pseudomonas aeruginosa virulence factor pyocyanin in wild-type cells by quorum sensing autoinducer-mimics", "creators": [ { "creatorType": "author", "firstName": "Bernardas", "lastName": "Morkunas" }, { "creatorType": "author", "firstName": "Warren R J D", "lastName": "Galloway" }, { "creatorType": "author", "firstName": "Megan", "lastName": "Wright" }, { "creatorType": "author", "firstName": "Brett M", "lastName": "Ibbeson" }, { "creatorType": "author", "firstName": "James T", "lastName": "Hodgkinson" }, { "creatorType": "author", "firstName": "Kieron M G", "lastName": "O'Connell" }, { "creatorType": "author", "firstName": "Noemi", "lastName": "Bartolucci" }, { "creatorType": "author", "firstName": "Martina", "lastName": "Della Valle" }, { "creatorType": "author", "firstName": "Martin", "lastName": "Welch" }, { "creatorType": "author", "firstName": "David R", "lastName": "Spring" } ], "abstractNote": "Pseudomonas aeruginosa is a notorious human pathogen associated with a range of life-threatening nosocomial infections. There is an increasing problem of antibiotic resistance in P. aeruginosa, highlighted by the emergence of multi-drug resistant strains. Thus the exploration of new strategies for the treatment of P. aeruginosa infections is clearly warranted. P. aeruginosa is known to produce a range of virulence factors that enhance its ability to damage the host tissue and cause disease. One of the most important virulence factors is pyocyanin. P. aeruginosa regulates pyocyanin production using an intercellular communication mechanism called quorum sensing, which is mediated by small signalling molecules termed autoinducers. One native autoinducer is N-(3-oxododecanoyl)-L-homoserine lactone (OdDHL). Herein we report the synthesis of a collection of abiotic OdDHL-mimics. A number of novel compounds capable of competing with the endogenous OdDHL and consequently, inhibiting the production of pyocyanin in cultures of wild type P. aeruginosa were identified. We present evidence suggesting that compounds of this general structural type act as direct antagonists of quorum sensing in P. aeruginosa and as such may find value as molecular tools for the study and manipulation of this signalling pathway. A direct quantitative comparison of the pyocyanin suppressive activities of the most active OdDHL-mimics with some previously-reported inhibitors (based around different general structural frameworks) of quorum sensing from the literature, was also made.", "publicationTitle": "Organic & biomolecular chemistry", "publisher": "", "place": "", "date": "Nov 14, 2012", "volume": "10", "issue": "42", "section": "", "partNumber": "", "partTitle": "", "pages": "8452-8464", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "Org. Biomol. Chem.", "DOI": "10.1039/c2ob26501j", "citationKey": "", "url": "", "accessDate": "", "PMID": "", "PMCID": "", "ISSN": "1477-0539", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "eng", "libraryCatalog": "NCBI PubMed", "callNumber": "", "rights": "", "extra": "PMID: 23014532", "tags": [ { "tag": "4-Butyrolactone", "type": 1 }, { "tag": "Homoserine", "type": 1 }, { "tag": "Humans", "type": 1 }, { "tag": "PSEUDOMONAS aeruginosa", "type": 1 }, { "tag": "Pseudomonas Infections", "type": 1 }, { "tag": "Pyocyanine", "type": 1 }, { "tag": "Quorum sensing", "type": 1 }, { "tag": "Small Molecule Libraries", "type": 1 }, { "tag": "Virulence Factors", "type": 1 } ], "collections": [], "relations": {}, "dateAdded": "2014-06-02T15:08:35Z", "dateModified": "2014-06-02T15:08:35Z" } }, { "key": "RMMQDDVK", "version": 96, "library": { "type": "group", "id": 252250, "name": "Summer Research Grant", "links": { "alternate": { "href": "https://www.zotero.org/groups/summer_research_grant", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/252250/items/RMMQDDVK", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/summer_research_grant/items/RMMQDDVK", "type": "text/html" } }, "meta": { "createdByUser": { "id": 1822692, "username": "cmorg13", "name": "", "links": { "alternate": { "href": "https://www.zotero.org/cmorg13", "type": "text/html" } } }, "creatorSummary": "Jeukens et al.", "parsedDate": "2014", "numChildren": 1 }, "data": { "key": "RMMQDDVK", "version": 96, "itemType": "journalArticle", "title": "Comparative Genomics of Isolates of a Pseudomonas aeruginosa Epidemic Strain Associated with Chronic Lung Infections of Cystic Fibrosis Patients", "creators": [ { "creatorType": "author", "firstName": "Julie", "lastName": "Jeukens" }, { "creatorType": "author", "firstName": "Brian", "lastName": "Boyle" }, { "creatorType": "author", "firstName": "Irena", "lastName": "Kukavica-Ibrulj" }, { "creatorType": "author", "firstName": "Myriam M", "lastName": "Ouellet" }, { "creatorType": "author", "firstName": "Shawn D", "lastName": "Aaron" }, { "creatorType": "author", "firstName": "Steve J", "lastName": "Charette" }, { "creatorType": "author", "firstName": "Joanne L", "lastName": "Fothergill" }, { "creatorType": "author", "firstName": "Nicholas P", "lastName": "Tucker" }, { "creatorType": "author", "firstName": "Craig", "lastName": "Winstanley" }, { "creatorType": "author", "firstName": "Roger C", "lastName": "Levesque" } ], "abstractNote": "Pseudomonas aeruginosa is the main cause of fatal chronic lung infections among individuals suffering from cystic fibrosis (CF). During the past 15 years, particularly aggressive strains transmitted among CF patients have been identified, initially in Europe and more recently in Canada. The aim of this study was to generate high-quality genome sequences for 7 isolates of the Liverpool epidemic strain (LES) from the United Kingdom and Canada representing different virulence characteristics in order to: (1) associate comparative genomics results with virulence factor variability and (2) identify genomic and/or phenotypic divergence between the two geographical locations. We performed phenotypic characterization of pyoverdine, pyocyanin, motility, biofilm formation, and proteolytic activity. We also assessed the degree of virulence using the Dictyostelium discoideum amoeba model. Comparative genomics analysis revealed at least one large deletion (40-50 kb) in 6 out of the 7 isolates compared to the reference genome of LESB58. These deletions correspond to prophages, which are known to increase the competitiveness of LESB58 in chronic lung infection. We also identified 308 non-synonymous polymorphisms, of which 28 were associated with virulence determinants and 52 with regulatory proteins. At the phenotypic level, isolates showed extensive variability in production of pyocyanin, pyoverdine, proteases and biofilm as well as in swimming motility, while being predominantly avirulent in the amoeba model. Isolates from the two continents were phylogenetically and phenotypically undistinguishable. Most regulatory mutations were isolate-specific and 29% of them were predicted to have high functional impact. Therefore, polymorphism in regulatory genes is likely to be an important basis for phenotypic diversity among LES isolates, which in turn might contribute to this strain's adaptability to varying conditions in the CF lung.", "publicationTitle": "PloS one", "publisher": "", "place": "", "date": "2014", "volume": "9", "issue": "2", "section": "", "partNumber": "", "partTitle": "", "pages": "e87611", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "PLoS ONE", "DOI": "10.1371/journal.pone.0087611", "citationKey": "", "url": "", "accessDate": "", "PMID": "", "PMCID": "", "ISSN": "1932-6203", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "eng", "libraryCatalog": "NCBI PubMed", "callNumber": "", "rights": "", "extra": "PMID: 24505294 \nPMCID: PMC3914812", "tags": [], "collections": [], "relations": {}, "dateAdded": "2014-06-02T15:08:35Z", "dateModified": "2014-06-02T15:08:35Z" } }, { "key": "XD9ACDN7", "version": 96, "library": { "type": "group", "id": 252250, "name": "Summer Research Grant", "links": { "alternate": { "href": "https://www.zotero.org/groups/summer_research_grant", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/252250/items/XD9ACDN7", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/summer_research_grant/items/XD9ACDN7", "type": "text/html" } }, "meta": { "createdByUser": { "id": 1822692, "username": "cmorg13", "name": "", "links": { "alternate": { "href": "https://www.zotero.org/cmorg13", "type": "text/html" } } }, "creatorSummary": "Jakobsen et al.", "parsedDate": "2012-05", "numChildren": 1 }, "data": { "key": "XD9ACDN7", "version": 96, "itemType": "journalArticle", "title": "Ajoene, a sulfur-rich molecule from garlic, inhibits genes controlled by quorum sensing", "creators": [ { "creatorType": "author", "firstName": "Tim Holm", "lastName": "Jakobsen" }, { "creatorType": "author", "firstName": "Maria", "lastName": "van Gennip" }, { "creatorType": "author", "firstName": "Richard Kerry", "lastName": "Phipps" }, { "creatorType": "author", "firstName": "Meenakshi Sundaram", "lastName": "Shanmugham" }, { "creatorType": "author", "firstName": "Louise Dahl", "lastName": "Christensen" }, { "creatorType": "author", "firstName": "Morten", "lastName": "Alhede" }, { "creatorType": "author", "firstName": "Mette Eline", "lastName": "Skindersoe" }, { "creatorType": "author", "firstName": "Thomas Bovbjerg", "lastName": "Rasmussen" }, { "creatorType": "author", "firstName": "Karlheinz", "lastName": "Friedrich" }, { "creatorType": "author", "firstName": "Friedrich", "lastName": "Uthe" }, { "creatorType": "author", "firstName": "Peter Østrup", "lastName": "Jensen" }, { "creatorType": "author", "firstName": "Claus", "lastName": "Moser" }, { "creatorType": "author", "firstName": "Kristian Fog", "lastName": "Nielsen" }, { "creatorType": "author", "firstName": "Leo", "lastName": "Eberl" }, { "creatorType": "author", "firstName": "Thomas Ostenfeld", "lastName": "Larsen" }, { "creatorType": "author", "firstName": "David", "lastName": "Tanner" }, { "creatorType": "author", "firstName": "Niels", "lastName": "Høiby" }, { "creatorType": "author", "firstName": "Thomas", "lastName": "Bjarnsholt" }, { "creatorType": "author", "firstName": "Michael", "lastName": "Givskov" } ], "abstractNote": "In relation to emerging multiresistant bacteria, development of antimicrobials and new treatment strategies of infections should be expected to become a high-priority research area. Quorum sensing (QS), a communication system used by pathogenic bacteria like Pseudomonas aeruginosa to synchronize the expression of specific genes involved in pathogenicity, is a possible drug target. Previous in vitro and in vivo studies revealed a significant inhibition of P. aeruginosa QS by crude garlic extract. By bioassay-guided fractionation of garlic extracts, we determined the primary QS inhibitor present in garlic to be ajoene, a sulfur-containing compound with potential as an antipathogenic drug. By comprehensive in vitro and in vivo studies, the effect of synthetic ajoene toward P. aeruginosa was elucidated. DNA microarray studies of ajoene-treated P. aeruginosa cultures revealed a concentration-dependent attenuation of a few but central QS-controlled virulence factors, including rhamnolipid. Furthermore, ajoene treatment of in vitro biofilms demonstrated a clear synergistic, antimicrobial effect with tobramycin on biofilm killing and a cease in lytic necrosis of polymorphonuclear leukocytes. Furthermore, in a mouse model of pulmonary infection, a significant clearing of infecting P. aeruginosa was detected in ajoene-treated mice compared to a nontreated control group. This study adds to the list of examples demonstrating the potential of QS-interfering compounds in the treatment of bacterial infections.", "publicationTitle": "Antimicrobial agents and chemotherapy", "publisher": "", "place": "", "date": "May 2012", "volume": "56", "issue": "5", "section": "", "partNumber": "", "partTitle": "", "pages": "2314-2325", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "Antimicrob. Agents Chemother.", "DOI": "10.1128/AAC.05919-11", "citationKey": "", "url": "", "accessDate": "", "PMID": "", "PMCID": "", "ISSN": "1098-6596", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "eng", "libraryCatalog": "NCBI PubMed", "callNumber": "", "rights": "", "extra": "PMID: 22314537 \nPMCID: PMC3346669", "tags": [ { "tag": "Animals", "type": 1 }, { "tag": "Anti-Bacterial Agents", "type": 1 }, { "tag": "BIOFILMS", "type": 1 }, { "tag": "Chemical Fractionation", "type": 1 }, { "tag": "Disulfides", "type": 1 }, { "tag": "Drug Synergism", "type": 1 }, { "tag": "Garlic", "type": 1 }, { "tag": "Gene Expression Regulation, Bacterial", "type": 1 }, { "tag": "Genes, Reporter", "type": 1 }, { "tag": "Glycolipids", "type": 1 }, { "tag": "Mice", "type": 1 }, { "tag": "Neutrophils", "type": 1 }, { "tag": "PSEUDOMONAS aeruginosa", "type": 1 }, { "tag": "Plant Extracts", "type": 1 }, { "tag": "Pseudomonas Infections", "type": 1 }, { "tag": "Quorum sensing", "type": 1 }, { "tag": "Respiratory Tract Infections", "type": 1 }, { "tag": "Tobramycin", "type": 1 }, { "tag": "Virulence Factors", "type": 1 } ], "collections": [], "relations": {}, "dateAdded": "2014-06-02T15:08:35Z", "dateModified": "2014-06-02T15:08:35Z" } }, { "key": "D8RAEVQZ", "version": 96, "library": { "type": "group", "id": 252250, "name": "Summer Research Grant", "links": { "alternate": { "href": "https://www.zotero.org/groups/summer_research_grant", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/252250/items/D8RAEVQZ", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/summer_research_grant/items/D8RAEVQZ", "type": "text/html" } }, "meta": { "createdByUser": { "id": 1822692, "username": "cmorg13", "name": "", "links": { "alternate": { "href": "https://www.zotero.org/cmorg13", "type": "text/html" } } }, "creatorSummary": "O'Loughlin et al.", "parsedDate": "2013-10-29", "numChildren": 1 }, "data": { "key": "D8RAEVQZ", "version": 96, "itemType": "journalArticle", "title": "A quorum-sensing inhibitor blocks Pseudomonas aeruginosa virulence and biofilm formation", "creators": [ { "creatorType": "author", "firstName": "Colleen T", "lastName": "O'Loughlin" }, { "creatorType": "author", "firstName": "Laura C", "lastName": "Miller" }, { "creatorType": "author", "firstName": "Albert", "lastName": "Siryaporn" }, { "creatorType": "author", "firstName": "Knut", "lastName": "Drescher" }, { "creatorType": "author", "firstName": "Martin F", "lastName": "Semmelhack" }, { "creatorType": "author", "firstName": "Bonnie L", "lastName": "Bassler" } ], "abstractNote": "Quorum sensing is a chemical communication process that bacteria use to regulate collective behaviors. Disabling quorum-sensing circuits with small molecules has been proposed as a potential strategy to prevent bacterial pathogenicity. The human pathogen Pseudomonas aeruginosa uses quorum sensing to control virulence and biofilm formation. Here, we analyze synthetic molecules for inhibition of the two P. aeruginosa quorum-sensing receptors, LasR and RhlR. Our most effective compound, meta-bromo-thiolactone (mBTL), inhibits both the production of the virulence factor pyocyanin and biofilm formation. mBTL also protects Caenorhabditis elegans and human lung epithelial cells from killing by P. aeruginosa. Both LasR and RhlR are partially inhibited by mBTL in vivo and in vitro; however, RhlR, not LasR, is the relevant in vivo target. More potent antagonists do not exhibit superior function in impeding virulence. Because LasR and RhlR reciprocally control crucial virulence factors, appropriately tuning rather than completely inhibiting their activities appears to hold the key to blocking pathogenesis in vivo.", "publicationTitle": "Proceedings of the National Academy of Sciences of the United States of America", "publisher": "", "place": "", "date": "Oct 29, 2013", "volume": "110", "issue": "44", "section": "", "partNumber": "", "partTitle": "", "pages": "17981-17986", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "Proc. Natl. Acad. Sci. U.S.A.", "DOI": "10.1073/pnas.1316981110", "citationKey": "", "url": "", "accessDate": "", "PMID": "", "PMCID": "", "ISSN": "1091-6490", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "eng", "libraryCatalog": "NCBI PubMed", "callNumber": "", "rights": "", "extra": "PMID: 24143808 \nPMCID: PMC3816427", "tags": [ { "tag": "Animals", "type": 1 }, { "tag": "BIOFILMS", "type": 1 }, { "tag": "Bacterial Proteins", "type": 1 }, { "tag": "Caenorhabditis elegans", "type": 1 }, { "tag": "Cell Line", "type": 1 }, { "tag": "Escherichia coli", "type": 1 }, { "tag": "Humans", "type": 1 }, { "tag": "Lactones", "type": 1 }, { "tag": "Microarray Analysis", "type": 1 }, { "tag": "Molecular Structure", "type": 1 }, { "tag": "PSEUDOMONAS aeruginosa", "type": 1 }, { "tag": "Pyocyanine", "type": 1 }, { "tag": "Quorum sensing", "type": 1 }, { "tag": "Respiratory Mucosa", "type": 1 }, { "tag": "Sulfur Compounds", "type": 1 }, { "tag": "Trans-Activators", "type": 1 }, { "tag": "Virulence", "type": 1 } ], "collections": [], "relations": {}, "dateAdded": "2014-06-02T15:08:35Z", "dateModified": "2014-06-02T15:08:35Z" } }, { "key": "KVK6FM4C", "version": 96, "library": { "type": "group", "id": 252250, "name": "Summer Research Grant", "links": { "alternate": { "href": "https://www.zotero.org/groups/summer_research_grant", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/252250/items/KVK6FM4C", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/summer_research_grant/items/KVK6FM4C", "type": "text/html" }, "up": { "href": "https://api.zotero.org/groups/252250/items/RKEV7CW4", "type": "application/json" } }, "meta": { "createdByUser": { "id": 1822692, "username": "cmorg13", "name": "", "links": { "alternate": { "href": "https://www.zotero.org/cmorg13", "type": "text/html" } } } }, "data": { "key": "KVK6FM4C", "version": 96, "parentItem": "RKEV7CW4", "itemType": "attachment", "linkMode": "linked_url", "title": 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