[ { "key": "7CUWHXRC", "version": 37, "library": { "type": "group", "id": 249844, "name": "RBC_Cytometry", "links": { "alternate": { "href": "https://www.zotero.org/groups/rbc_cytometry", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/249844/items/7CUWHXRC", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/rbc_cytometry/items/7CUWHXRC", "type": "text/html" } }, "meta": { "createdByUser": { "id": 46158, "username": "fyshou", "name": "", "links": { "alternate": { "href": "https://www.zotero.org/fyshou", "type": "text/html" } } }, "creatorSummary": "Malleret et al.", "parsedDate": "2013", "numChildren": 1 }, "data": { "key": "7CUWHXRC", "version": 37, "itemType": "journalArticle", "title": "Significant biochemical, biophysical and metabolic diversity in circulating human cord blood reticulocytes", "creators": [ { "creatorType": "author", "firstName": "Benoît", "lastName": "Malleret" }, { "creatorType": "author", "firstName": "Fenggao", "lastName": "Xu" }, { "creatorType": "author", "firstName": "Narla", "lastName": "Mohandas" }, { "creatorType": "author", "firstName": "Rossarin", "lastName": "Suwanarusk" }, { "creatorType": "author", "firstName": "Cindy", "lastName": "Chu" }, { "creatorType": "author", "firstName": "Juliana A", "lastName": "Leite" }, { "creatorType": "author", "firstName": "Kayen", "lastName": "Low" }, { "creatorType": "author", "firstName": "Claudia", "lastName": "Turner" }, { "creatorType": "author", "firstName": "Kanlaya", "lastName": "Sriprawat" }, { "creatorType": "author", "firstName": "Rou", "lastName": "Zhang" }, { "creatorType": "author", "firstName": "Olivier", "lastName": "Bertrand" }, { "creatorType": "author", "firstName": "Yves", "lastName": "Colin" }, { "creatorType": "author", "firstName": "Fabio T M", "lastName": "Costa" }, { "creatorType": "author", "firstName": "Choon Nam", "lastName": "Ong" }, { "creatorType": "author", "firstName": "Mah Lee", "lastName": "Ng" }, { "creatorType": "author", "firstName": "Chwee Teck", "lastName": "Lim" }, { "creatorType": "author", "firstName": "Francois", "lastName": "Nosten" }, { "creatorType": "author", "firstName": "Laurent", "lastName": "Rénia" }, { "creatorType": "author", "firstName": "Bruce", "lastName": "Russell" } ], "abstractNote": "BACKGROUND: The transition from enucleated reticulocytes to mature normocytes is marked by substantial remodeling of the erythrocytic cytoplasm and membrane. Despite conspicuous changes, most studies describe the maturing reticulocyte as a homogenous erythropoietic cell type. While reticulocyte staging based on fluorescent RNA stains such as thiazole orange have been useful in a clinical setting; these 'sub-vital' stains may confound delicate studies on reticulocyte biology and may preclude their use in heamoparasite invasion studies.\nDESIGN AND METHODS: Here we use highly purified populations of reticulocytes isolated from cord blood, sorted by flow cytometry into four sequential subpopulations based on transferrin receptor (CD71) expression: CD71high, CD71medium, CD71low and CD71negative. Each of these subgroups was phenotyped in terms of their, morphology, membrane antigens, biomechanical properties and metabolomic profile.\nRESULTS: Superficially CD71high and CD71medium reticulocytes share a similar gross morphology (large and multilobular) when compared to the smaller, smooth and increasingly concave reticulocytes as seen in the in the CD71low and CD71negativesamples. However, between each of the four sample sets we observe significant decreases in shear modulus, cytoadhesive capacity, erythroid receptor expression (CD44, CD55, CD147, CD235R, and CD242) and metabolite concentrations. Interestingly increasing amounts of boric acid was found in the mature reticulocytes.\nCONCLUSIONS: Reticulocyte maturation is a dynamic and continuous process, confounding efforts to rigidly classify them. Certainly this study does not offer an alternative classification strategy; instead we used a nondestructive sampling method to examine key phenotypic changes of in reticulocytes. Our study emphasizes a need to focus greater attention on reticulocyte biology.", "publicationTitle": "PloS one", "publisher": "", "place": "", "date": "2013", "volume": "8", "issue": "10", "section": "", "partNumber": "", "partTitle": "", "pages": "e76062", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "PLoS ONE", "DOI": "10.1371/journal.pone.0076062", "citationKey": "", "url": "", "accessDate": "", "PMID": "", "PMCID": "", "ISSN": "1932-6203", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "eng", "libraryCatalog": "NCBI PubMed", "callNumber": "", "rights": "", "extra": "PMID: 24116088 \nPMCID: PMC3793000", "tags": [], "collections": [ "UVFKH62W" ], "relations": {}, "dateAdded": "2014-03-31T03:19:58Z", "dateModified": "2014-03-31T03:19:58Z" } }, { "key": "MCB36C9E", "version": 37, "library": { "type": "group", "id": 249844, "name": "RBC_Cytometry", "links": { "alternate": { "href": "https://www.zotero.org/groups/rbc_cytometry", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/249844/items/MCB36C9E", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/rbc_cytometry/items/MCB36C9E", "type": "text/html" }, "up": { "href": "https://api.zotero.org/groups/249844/items/7CUWHXRC", "type": "application/json" } }, "meta": { "createdByUser": { "id": 46158, "username": "fyshou", "name": "", "links": { "alternate": { "href": "https://www.zotero.org/fyshou", "type": "text/html" } } } }, "data": { "key": "MCB36C9E", "version": 37, "parentItem": "7CUWHXRC", "itemType": "attachment", "linkMode": "linked_url", "title": "PubMed entry", "accessDate": "2014-03-31T03:19:58Z", "url": "http://www.ncbi.nlm.nih.gov/pubmed/24116088", "note": "", "contentType": "text/html", "charset": "", "tags": [], "relations": {}, "dateAdded": "2014-03-31T03:19:58Z", "dateModified": "2014-03-31T03:19:58Z" } }, { "key": "S6WQ9ZHF", "version": 36, "library": { "type": "group", "id": 249844, "name": "RBC_Cytometry", "links": { "alternate": { "href": "https://www.zotero.org/groups/rbc_cytometry", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/249844/items/S6WQ9ZHF", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/rbc_cytometry/items/S6WQ9ZHF", "type": "text/html" }, "up": { "href": "https://api.zotero.org/groups/249844/items/K4FJV8JS", "type": "application/json" } }, "meta": { "createdByUser": { "id": 46158, "username": "fyshou", "name": "", "links": { "alternate": { "href": "https://www.zotero.org/fyshou", "type": "text/html" } } } }, "data": { "key": "S6WQ9ZHF", "version": 36, "parentItem": "K4FJV8JS", "itemType": "attachment", "linkMode": "linked_url", "title": "PubMed entry", "accessDate": "2014-03-31T02:44:24Z", "url": "http://www.ncbi.nlm.nih.gov/pubmed/15727926", "note": "", "contentType": "text/html", "charset": "", "tags": [], "relations": {}, "dateAdded": "2014-03-31T02:44:24Z", "dateModified": "2014-03-31T02:44:24Z" } }, { "key": "K4FJV8JS", "version": 36, "library": { "type": "group", "id": 249844, "name": "RBC_Cytometry", "links": { "alternate": { "href": "https://www.zotero.org/groups/rbc_cytometry", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/249844/items/K4FJV8JS", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/rbc_cytometry/items/K4FJV8JS", "type": "text/html" } }, "meta": { "createdByUser": { "id": 46158, "username": "fyshou", "name": "", "links": { "alternate": { "href": "https://www.zotero.org/fyshou", "type": "text/html" } } }, "creatorSummary": "Kucinskiene et al.", "parsedDate": "2005-03", "numChildren": 1 }, "data": { "key": "K4FJV8JS", "version": 36, "itemType": "journalArticle", "title": "Flow cytometric evaluation of bound IgG on erythrocytes of anaemic dogs", "creators": [ { "creatorType": "author", "firstName": "Gintare", "lastName": "Kucinskiene" }, { "creatorType": "author", "firstName": "Hans-Joachim", "lastName": "Schuberth" }, { "creatorType": "author", "firstName": "Wolfgang", "lastName": "Leibold" }, { "creatorType": "author", "firstName": "Juozas", "lastName": "Pieskus" } ], "abstractNote": "The detection of red blood cell (RBC)-bound immunoglobulins in case of anaemia with the direct agglutination test (DAT or Coombs test) has been reported to be of low sensitivity. We therefore tested the applicability of flow cytometry for the detection of canine IgG on RBC using two different IgG-specific secondary reagents: goat-anti-dog IgG (GalphaD-IgG) and rabbit-anti-dog IgG (RalphaD-IgG). Membrane staining RBC samples were performed at 4 degrees C. Comparisons of agglutination test at 37 degrees C and 4 degrees C showed, that binding of the secondary antibodies at 4 degrees C was more sensitive compared to agglutination at 37 degrees C and the two antisera differed considerable in their agglutination activity. Binding of GalphaD-IgG and RalphaD-IgG to RBC of healthy dogs (n=15) was low and mean fluorescence intensities were taken to calculate thresholds above which RBC of patients were judged positive. As in agglutination tests, both secondary antisera displayed considerable differences (concentration-dependent binding and histogram profiles) after flow cytometric analysis. Using flow cytometry, with GalphaD-IgG 8 of 17 agglutination-negative patients were positive and RalphaD-IgG was positive with 3 of 3 agglutination-negative RBC samples. Thus, flow cytometric analysis of proved to be a sensitive technique, detecting RBC-bound canine IgG of DAT-negative patients. The results of both techniques, however, are significantly influenced by the used IgG-specific polyclonal reagents.", "publicationTitle": "Veterinary journal (London, England: 1997)", "publisher": "", "place": "", "date": "Mar 2005", "volume": "169", "issue": "2", "section": "", "partNumber": "", "partTitle": "", "pages": "303-307", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "Vet. J.", "DOI": "10.1016/j.tvjl.2004.01.011", "citationKey": "", "url": "", "accessDate": "", "PMID": "", "PMCID": "", "ISSN": "1090-0233", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "eng", "libraryCatalog": "NCBI PubMed", "callNumber": "", "rights": "", "extra": "PMID: 15727926", "tags": [ { "tag": "Agglutination Tests", "type": 1 }, { "tag": "Anemia, Hemolytic, Autoimmune", "type": 1 }, { "tag": "Animals", "type": 1 }, { "tag": "Autoantibodies", "type": 1 }, { "tag": "Dog Diseases", "type": 1 }, { "tag": "Dogs", "type": 1 }, { "tag": "Erythrocytes", "type": 1 }, { "tag": "Female", "type": 1 }, { "tag": "Flow Cytometry", "type": 1 }, { "tag": "Immunoglobulin G", "type": 1 }, { "tag": "Male", "type": 1 }, { "tag": "Predictive Value of Tests", "type": 1 }, { "tag": "Sensitivity and Specificity", "type": 1 } ], "collections": [ "XRXBJVQD" ], "relations": {}, "dateAdded": "2014-03-31T02:44:24Z", "dateModified": "2014-03-31T02:44:24Z" } }, { "key": "VHWB54MM", "version": 35, "library": { "type": "group", "id": 249844, "name": "RBC_Cytometry", "links": { "alternate": { "href": "https://www.zotero.org/groups/rbc_cytometry", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/249844/items/VHWB54MM", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/rbc_cytometry/items/VHWB54MM", "type": "text/html" } }, "meta": { "createdByUser": { "id": 46158, "username": "fyshou", "name": "", "links": { "alternate": { "href": "https://www.zotero.org/fyshou", "type": "text/html" } } }, "creatorSummary": "Chaudhary et al.", "parsedDate": "2006-08", "numChildren": 1 }, "data": { "key": "VHWB54MM", "version": 35, "itemType": "journalArticle", "title": "Application of flow cytometry in detection of red-cell-bound IgG in Coombs-negative AIHA", "creators": [ { "creatorType": "author", "firstName": "Rajendra", "lastName": "Chaudhary" }, { "creatorType": "author", "firstName": "Sudipta S", "lastName": "Das" }, { "creatorType": "author", "firstName": "Ritu", "lastName": "Gupta" }, { "creatorType": "author", "firstName": "Dheeraj", "lastName": "Khetan" } ], "abstractNote": "Coombs negative autoimmune hemolytic anemia (AIHA) is characterized by laboratory evidence of in vivo hemolysis along with a negative direct antiglobulin test (DAT) performed by conventional tube technique (CTT) in clinically suspected AIHA patients. The sensitive gel test (GT) and flow cytometry (FC) can effectively diagnose such patients where CTT does not detect low level of red cell autoantibodies. We investigated the use of FC in the serological evaluation of CTT DAT negative AIHA and its comparison with GT DAT. Of the 50 patients with suspected AIHA, CTT DAT was negative in 5 patients (Coombs negative AIHA). GT DAT could detect red cell autoantibodies in 4 of these 5 patients. Monospecific GT DAT showed IgG and/or C3d as the responsible autoantibody. FC was considered as reactive when MFI was >3.6 (mean of 20 healthy negative volunteers +2SD). FC was reactive in all five Coombs negative AIHA patients. The mean MFI in five known CTT DAT positive samples taken for comparison was significantly higher compared to 5 DAT negative AIHA (18.3 +/- 7.78 vs. 7.88 +/- 1.35, p < 0.05). There was poor correlation between strength of GT DAT and MFI by FC. We conclude that FC is more sensitive test than the CTT and helps in the serological diagnosis of Coombs negative AIHA. However, in resource poor settings, GT DAT can be a good alternative to FC.", "publicationTitle": "Hematology (Amsterdam, Netherlands)", "publisher": "", "place": "", "date": "Aug 2006", "volume": "11", "issue": "4", "section": "", "partNumber": "", "partTitle": "", "pages": "295-300", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "Hematology", "DOI": "10.1080/10245330600915958", "citationKey": "", "url": "", "accessDate": "", "PMID": "", "PMCID": "", "ISSN": "1607-8454", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "eng", "libraryCatalog": "NCBI PubMed", "callNumber": "", "rights": "", "extra": "PMID: 17178671", "tags": [ { "tag": "Adolescent", "type": 1 }, { "tag": "Adult", "type": 1 }, { "tag": "Anemia, Hemolytic, Autoimmune", "type": 1 }, { "tag": "Antibodies, Anti-Idiotypic", "type": 1 }, { "tag": "Arthritis, Rheumatoid", "type": 1 }, { "tag": "Complement C3d", "type": 1 }, { "tag": "Coombs Test", "type": 1 }, { "tag": "Developing Countries", "type": 1 }, { "tag": "Erythrocytes", "type": 1 }, { "tag": "False Negative Reactions", "type": 1 }, { "tag": "Female", "type": 1 }, { "tag": "Flow Cytometry", "type": 1 }, { "tag": "Hemolytic Plaque Technique", "type": 1 }, { "tag": "Hepatitis, Autoimmune", "type": 1 }, { "tag": "Humans", "type": 1 }, { "tag": "Immunoglobulin G", "type": 1 }, { "tag": "Incidence", "type": 1 }, { "tag": "India", "type": 1 }, { "tag": "Male", "type": 1 }, { "tag": "Middle Aged", "type": 1 }, { "tag": "Prospective Studies", "type": 1 }, { "tag": "Sensitivity and Specificity", "type": 1 } ], "collections": [ "XRXBJVQD" ], "relations": {}, "dateAdded": "2014-03-31T02:42:28Z", "dateModified": "2014-03-31T02:42:28Z" } }, { "key": "KJ5CATIV", "version": 35, "library": { "type": "group", "id": 249844, "name": "RBC_Cytometry", "links": { "alternate": { "href": "https://www.zotero.org/groups/rbc_cytometry", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/249844/items/KJ5CATIV", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/rbc_cytometry/items/KJ5CATIV", "type": "text/html" }, "up": { "href": "https://api.zotero.org/groups/249844/items/VHWB54MM", "type": "application/json" } }, "meta": { "createdByUser": { "id": 46158, "username": "fyshou", "name": "", "links": { "alternate": { "href": "https://www.zotero.org/fyshou", "type": "text/html" } } } }, "data": { "key": "KJ5CATIV", "version": 35, "parentItem": "VHWB54MM", "itemType": "attachment", "linkMode": "linked_url", "title": "PubMed entry", "accessDate": "2014-03-31T02:42:28Z", "url": "http://www.ncbi.nlm.nih.gov/pubmed/17178671", "note": "", "contentType": "text/html", "charset": "", "tags": [], "relations": {}, "dateAdded": "2014-03-31T02:42:28Z", "dateModified": "2014-03-31T02:42:28Z" } }, { "key": "72QMFNE6", "version": 34, "library": { "type": "group", "id": 249844, "name": "RBC_Cytometry", "links": { "alternate": { "href": "https://www.zotero.org/groups/rbc_cytometry", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/249844/items/72QMFNE6", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/rbc_cytometry/items/72QMFNE6", "type": "text/html" }, "up": { "href": "https://api.zotero.org/groups/249844/items/W8XEFF6C", "type": "application/json" } }, "meta": { "createdByUser": { "id": 46158, "username": "fyshou", "name": "", "links": { "alternate": { "href": "https://www.zotero.org/fyshou", "type": "text/html" } } } }, "data": { "key": "72QMFNE6", "version": 34, "parentItem": "W8XEFF6C", "itemType": "attachment", "linkMode": "linked_url", "title": "PubMed entry", "accessDate": "2014-03-31T02:40:34Z", "url": "http://www.ncbi.nlm.nih.gov/pubmed/19320864", "note": "", "contentType": "text/html", "charset": "", "tags": [], "relations": {}, "dateAdded": "2014-03-31T02:40:34Z", "dateModified": "2014-03-31T02:40:34Z" } }, { "key": "W8XEFF6C", "version": 34, "library": { "type": "group", "id": 249844, "name": "RBC_Cytometry", "links": { "alternate": { "href": "https://www.zotero.org/groups/rbc_cytometry", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/249844/items/W8XEFF6C", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/rbc_cytometry/items/W8XEFF6C", "type": "text/html" } }, "meta": { "createdByUser": { "id": 46158, "username": "fyshou", "name": "", "links": { "alternate": { "href": "https://www.zotero.org/fyshou", "type": "text/html" } } }, "creatorSummary": "Lin et al.", "parsedDate": "2009-07", "numChildren": 1 }, "data": { "key": "W8XEFF6C", "version": 34, "itemType": "journalArticle", "title": "Clinical application of a flow cytometric direct antiglobulin test", "creators": [ { "creatorType": "author", "firstName": "Jeong-Shi", "lastName": "Lin" }, { "creatorType": "author", "firstName": "Tsung-Chi", "lastName": "Hao" }, { "creatorType": "author", "firstName": "Jau-Yi", "lastName": "Lyou" }, { "creatorType": "author", "firstName": "Ying-Ju", "lastName": "Chen" }, { "creatorType": "author", "firstName": "Hsueng-Mei", "lastName": "Liu" }, { "creatorType": "author", "firstName": "Cheng-Hwai", "lastName": "Tzeng" }, { "creatorType": "author", "firstName": "Tzeon-Jye", "lastName": "Chiou" } ], "abstractNote": "BACKGROUND: The clinical application of flow cytometric direct antiglobulin test (FC-DAT) has rarely been evaluated for patients with various diseases including immune and nonimmune hemolytic anemia.\nSTUDY DESIGN AND METHODS: Blood samples from 380 patients with a variety of diseases were studied using the tube direct DAT and FC-DAT. The results of tube DAT and FC-DAT were compared. The predictive values of DAT for hemolysis were evaluated.\nRESULTS: Of 57 patients with autoimmune hemolytic anemia (AIHA), 6 of the 17 with a negative tube DAT (immunoglobulin G [IgG]) had a positive FC-DAT (IgG) and 23 of the 36 patients with a negative tube DAT (complement 3d [C3d]) had a positive FC-DAT (C3d). In 57 patients with AIHA, the incidence of positive results of FC-DAT (IgG) and tube DAT (IgG) were similar (42 positive vs. 40 positive); but in 323 patients without AIHA, the incidence of positive FC-DATs (IgG) was higher than that of tube DAT (IgG; 47 positive vs. 9 positive). The higher incidence of positive FC-DAT (C3d) than that of tube DAT (C3d) was seen in patients with AIHA (42 positive vs. 21 positive) as well as in patients without AIHA (61 positive vs. 5 positive). Both DAT (IgG) and DAT (C3d) positive has highest positive predictive value for hemolysis, followed by DAT (IgG) alone positive and DAT (C3d) alone positive.\nCONCLUSIONS: FC-DAT is a complementary test for diagnosing AIHA. There is a synergistic effect of the red blood cell-bound IgG and complement in predicting hemolysis.", "publicationTitle": "Transfusion", "publisher": "", "place": "", "date": "Jul 2009", "volume": "49", "issue": "7", "section": "", "partNumber": "", "partTitle": "", "pages": "1335-1346", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "Transfusion", "DOI": "10.1111/j.1537-2995.2009.02130.x", "citationKey": "", "url": "", "accessDate": "", "PMID": "", "PMCID": "", "ISSN": "1537-2995", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "eng", "libraryCatalog": "NCBI PubMed", "callNumber": "", "rights": "", "extra": "PMID: 19320864", "tags": [ { "tag": "Adult", "type": 1 }, { "tag": "Aged", "type": 1 }, { "tag": "Aged, 80 and over", "type": 1 }, { "tag": "Anemia, Hemolytic, Autoimmune", "type": 1 }, { "tag": "Coombs Test", "type": 1 }, { "tag": "Female", "type": 1 }, { "tag": "Flow Cytometry", "type": 1 }, { "tag": "Hemolysis", "type": 1 }, { "tag": "Humans", "type": 1 }, { "tag": "Male", "type": 1 }, { "tag": "Middle Aged", "type": 1 } ], "collections": [ "XRXBJVQD" ], "relations": {}, "dateAdded": "2014-03-31T02:40:34Z", "dateModified": "2014-03-31T02:40:34Z" } }, { "key": "PFTK6M8N", "version": 33, "library": { "type": "group", "id": 249844, "name": "RBC_Cytometry", "links": { "alternate": { "href": "https://www.zotero.org/groups/rbc_cytometry", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/249844/items/PFTK6M8N", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/rbc_cytometry/items/PFTK6M8N", "type": "text/html" }, "up": { "href": "https://api.zotero.org/groups/249844/items/SRXGBUCR", "type": "application/json" } }, "meta": { "createdByUser": { "id": 46158, "username": "fyshou", "name": "", "links": { "alternate": { "href": "https://www.zotero.org/fyshou", "type": "text/html" } } } }, "data": { "key": "PFTK6M8N", "version": 33, "parentItem": "SRXGBUCR", "itemType": "attachment", "linkMode": "linked_url", "title": "PubMed entry", "accessDate": "2014-03-31T02:20:11Z", "url": "http://www.ncbi.nlm.nih.gov/pubmed/6188505", "note": "", "contentType": "text/html", "charset": "", "tags": [], "relations": {}, "dateAdded": "2014-03-31T02:20:11Z", "dateModified": "2014-03-31T02:20:11Z" } }, { "key": "SRXGBUCR", "version": 33, "library": { "type": "group", "id": 249844, "name": "RBC_Cytometry", "links": { "alternate": { "href": "https://www.zotero.org/groups/rbc_cytometry", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/249844/items/SRXGBUCR", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/rbc_cytometry/items/SRXGBUCR", "type": "text/html" } }, "meta": { "createdByUser": { "id": 46158, "username": "fyshou", "name": "", "links": { "alternate": { "href": "https://www.zotero.org/fyshou", "type": "text/html" } } }, "creatorSummary": "Tanke et al.", "parsedDate": "1983-06", "numChildren": 1 }, "data": { "key": "SRXGBUCR", "version": 33, "itemType": "journalArticle", "title": "Flow cytometry of reticulocytes applied to clinical hematology", "creators": [ { "creatorType": "author", "firstName": "H J", "lastName": "Tanke" }, { "creatorType": "author", "firstName": "P H", "lastName": "Rothbarth" }, { "creatorType": "author", "firstName": "J M", "lastName": "Vossen" }, { "creatorType": "author", "firstName": "G J", "lastName": "Koper" }, { "creatorType": "author", "firstName": "J S", "lastName": "Ploem" } ], "abstractNote": "Reticulocytes in fixed human blood samples were stained for RNA with the fluorescent dye pyronin Y and measured by flow cytometry. The resulting relative frequency distributions of the RNA fluorescence intensities concurred with the different stages in maturation from early reticulocytes to mature red cells. A computer program was written to calculate from these frequency distributions the relative number of reticulocytes, their relative RNA content, and the median of the reticulocyte population (RNA index). This method was applied to 30 healthy blood bank donors (control group), as well as to patients with various hematologic disorders showing abnormal erythropoietic activity. The measured percentage of reticulocytes, RNA content, and RNA index were found to correlate well with the various hematologic disorders. Changes in erythropoiesis could be clearly followed, as was demonstrated by analyzing blood samples from children with aplastic anemia or acute myeloid leukemia, who were treated with allogeneic bone marrow transplantation. Measurements on blood samples from healthy blood bank donors showed that with this method, small changes in the reticulocyte population, such as the appearance of polychromatic erythrocytes in the peripheral blood 5-8 hr after donation, can be detected. The statistical reliability and the information provided on the maturation stage of the entire reticulocyte population make flow cytometry of peripheral blood reticulocytes a more informative method for the study of hematologic abnormalities than conventional methods for reticulocyte counting and classification.", "publicationTitle": "Blood", "publisher": "", "place": "", "date": "Jun 1983", "volume": "61", "issue": "6", "section": "", "partNumber": "", "partTitle": "", "pages": "1091-1097", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "Blood", "DOI": "", "citationKey": "", "url": "", "accessDate": "", "PMID": "", "PMCID": "", "ISSN": "0006-4971", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "eng", "libraryCatalog": "NCBI PubMed", "callNumber": "", "rights": "", "extra": "PMID: 6188505", "tags": [ { "tag": "Anemia, Aplastic", "type": 1 }, { "tag": "Blood Donors", "type": 1 }, { "tag": "Bone Marrow Transplantation", "type": 1 }, { "tag": "Cell Count", "type": 1 }, { "tag": "Cell Separation", "type": 1 }, { "tag": "Erythrocyte Aging", "type": 1 }, { "tag": "Erythropoiesis", "type": 1 }, { "tag": "Flow Cytometry", "type": 1 }, { "tag": "Humans", "type": 1 }, { "tag": "Leukemia, Myeloid, Acute", "type": 1 }, { "tag": "RNA", "type": 1 }, { "tag": "Reticulocytes", "type": 1 }, { "tag": "Thalassemia", "type": 1 } ], "collections": [ "UVFKH62W" ], "relations": {}, "dateAdded": "2014-03-31T02:20:11Z", "dateModified": "2014-03-31T02:20:11Z" } }, { "key": "DVE262NV", "version": 32, "library": { "type": "group", "id": 249844, "name": "RBC_Cytometry", "links": { "alternate": { "href": "https://www.zotero.org/groups/rbc_cytometry", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/249844/items/DVE262NV", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/rbc_cytometry/items/DVE262NV", "type": "text/html" } }, "meta": { "createdByUser": { "id": 46158, "username": "fyshou", "name": "", "links": { "alternate": { "href": "https://www.zotero.org/fyshou", "type": "text/html" } } }, "creatorSummary": "Davis", "parsedDate": "2001-12", "numChildren": 1 }, "data": { "key": "DVE262NV", "version": 32, "itemType": "journalArticle", "title": "Diagnostic utility of red cell flow cytometric analysis", "creators": [ { "creatorType": "author", "firstName": "B H", "lastName": "Davis" } ], "abstractNote": "Although most of the diagnostic applications of flow cytometry bring forth examples of leukocyte immunophenotyping for immunodeficiency diseases and leukemia-lymphoma diagnosis, the same technology has improved medical assessment of diseases affecting the red cell and erythropoiesis. Flow cytometric methods were first applied to laboratory hematology with the improvement in reticulocyte counting and the creation of the immature reticulocyte fraction for better anemia evaluation and therapeutic monitoring. A more recent improvement attributable to flow cytometry is accurate detection of fetal red cells in the evaluation of FMH hemorrhage. The same method used in the detection of fetal RBCs based on HbF content measurement using monoclonal antibodies also offers the potential for enumeration of F cells, which promises to have use in therapeutic monitoring of patients with sickle cell disease and the evaluation of other hemoglobinopathies and myelodysplasia. Other clinical uses of flow cytometric RBC analysis include nonisotopic red cell survival studies, sensitive blood group typing, sensitive detection of immune-mediated hemolytic diseases, and evaluation of parasitic diseases whose life cycle involves intracellular RBC infestation. This article summarizes red cell flow cytometry, particularly as it impacts the areas of immunohematology and laboratory hematology, and points to areas of potential future contribution of this technology to diagnostic medicine.", "publicationTitle": "Clinics in laboratory medicine", "publisher": "", "place": "", "date": "Dec 2001", "volume": "21", "issue": "4", "section": "", "partNumber": "", "partTitle": "", "pages": "829-840", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "Clin. Lab. Med.", "DOI": "", "citationKey": "", "url": "", "accessDate": "", "PMID": "", "PMCID": "", "ISSN": "0272-2712", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "eng", "libraryCatalog": "NCBI PubMed", "callNumber": "", "rights": "", "extra": "PMID: 11770290", "tags": [ { "tag": "Adult", "type": 1 }, { "tag": "Antigens, Surface", "type": 1 }, { "tag": "Erythrocytes, Abnormal", "type": 1 }, { "tag": "Erythropoiesis", "type": 1 }, { "tag": "Female", "type": 1 }, { "tag": "Fetomaternal Transfusion", "type": 1 }, { "tag": "Flow Cytometry", "type": 1 }, { "tag": "Hematologic Diseases", "type": 1 }, { "tag": "Hemoglobins", "type": 1 }, { "tag": "Humans", "type": 1 }, { "tag": "Pregnancy", "type": 1 }, { "tag": "Reproducibility of Results", "type": 1 }, { "tag": "Sensitivity and Specificity", "type": 1 } ], "collections": [], "relations": {}, "dateAdded": "2014-03-31T02:00:06Z", "dateModified": "2014-03-31T02:00:06Z" } }, { "key": "PAD34NM8", "version": 32, "library": { "type": "group", "id": 249844, "name": "RBC_Cytometry", "links": { "alternate": { "href": "https://www.zotero.org/groups/rbc_cytometry", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/249844/items/PAD34NM8", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/rbc_cytometry/items/PAD34NM8", "type": "text/html" }, "up": { "href": "https://api.zotero.org/groups/249844/items/DVE262NV", "type": "application/json" } }, "meta": { "createdByUser": { "id": 46158, "username": "fyshou", "name": "", "links": { "alternate": { "href": "https://www.zotero.org/fyshou", "type": "text/html" } } } }, "data": { "key": "PAD34NM8", "version": 32, "parentItem": "DVE262NV", "itemType": "attachment", "linkMode": "linked_url", "title": "PubMed entry", "accessDate": "2014-03-31T02:00:06Z", "url": "http://www.ncbi.nlm.nih.gov/pubmed/11770290", "note": "", "contentType": "text/html", "charset": "", "tags": [], "relations": {}, "dateAdded": "2014-03-31T02:00:06Z", "dateModified": "2014-03-31T02:00:06Z" } }, { "key": "PU37B5J9", "version": 31, "library": { "type": "group", "id": 249844, "name": "RBC_Cytometry", "links": { "alternate": { "href": "https://www.zotero.org/groups/rbc_cytometry", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/249844/items/PU37B5J9", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/rbc_cytometry/items/PU37B5J9", "type": "text/html" }, "up": { "href": "https://api.zotero.org/groups/249844/items/JQXH8PRR", "type": "application/json" } }, "meta": { "createdByUser": { "id": 46158, "username": "fyshou", "name": "", "links": { "alternate": { "href": "https://www.zotero.org/fyshou", "type": "text/html" } } } }, "data": { "key": "PU37B5J9", "version": 31, "parentItem": "JQXH8PRR", "itemType": "attachment", "linkMode": "linked_url", "title": "PubMed entry", "accessDate": "2014-03-31T01:31:34Z", "url": "http://www.ncbi.nlm.nih.gov/pubmed/18302186", "note": "", "contentType": "text/html", "charset": "", "tags": [], "relations": {}, "dateAdded": "2014-03-31T01:31:34Z", "dateModified": "2014-03-31T01:31:34Z" } }, { "key": "JQXH8PRR", "version": 31, "library": { "type": "group", "id": 249844, "name": "RBC_Cytometry", "links": { "alternate": { "href": "https://www.zotero.org/groups/rbc_cytometry", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/249844/items/JQXH8PRR", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/rbc_cytometry/items/JQXH8PRR", "type": "text/html" } }, "meta": { "createdByUser": { "id": 46158, "username": "fyshou", "name": "", "links": { "alternate": { "href": "https://www.zotero.org/fyshou", "type": "text/html" } } }, "creatorSummary": "Grimberg et al.", "parsedDate": "2008-06", "numChildren": 1 }, "data": { "key": "JQXH8PRR", "version": 31, "itemType": "journalArticle", "title": "Monitoring Plasmodium falciparum growth and development by UV flow cytometry using an optimized Hoechst-thiazole orange staining strategy", "creators": [ { "creatorType": "author", "firstName": "Brian T", "lastName": "Grimberg" }, { "creatorType": "author", "firstName": "John J", "lastName": "Erickson" }, { "creatorType": "author", "firstName": "R Michael", "lastName": "Sramkoski" }, { "creatorType": "author", "firstName": "James W", "lastName": "Jacobberger" }, { "creatorType": "author", "firstName": "Peter A", "lastName": "Zimmerman" } ], "abstractNote": "The complex life cycle of Plasmodium falciparum (Pf) makes it difficult to limit infections and reduce the risk of severe malaria. Improved understanding of Pf blood-stage growth and development would provide new opportunities to evaluate and interfere with successful completion of the parasite's life cycle. Cultured blood stage Pf was incubated with Hoechst 33342 (HO) and thiazole orange (TO) to stain DNA and total nucleic acids, respectively. Correlated HO and TO fluorescence emissions were then measured by flow cytometry. Complex bivariate data patterns were analyzed by manual cluster gating to quantify parasite life cycle stages. The permutations of viable staining with both reagents were tested for optimal detection of parasitized RBC (pRBC). Pf cultures were exposed to HO and TO simultaneously to achieve optimal staining of pRBC and consistent quantification of early and late stages of the replicative cycle (rings through schizonts). Staining of Pf nucleic acids allows for analysis of parasite development in the absence of fixatives, lysis, or radioactivity to enable examination of erythrocytes from parasite invasion through schizont rupture using sensitive and rapid assay procedures. Investigation of the mechanisms by which anti-malarial drugs and antibodies act against different Pf lifecycle stages will be aided by this cytometric strategy.", "publicationTitle": "Cytometry. Part A: the journal of the International Society for Analytical Cytology", "publisher": "", "place": "", "date": "Jun 2008", "volume": "73", "issue": "6", "section": "", "partNumber": "", "partTitle": "", "pages": "546-554", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "Cytometry A", "DOI": "10.1002/cyto.a.20541", "citationKey": "", "url": "", "accessDate": "", "PMID": "", "PMCID": "", "ISSN": "1552-4930", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "eng", "libraryCatalog": "NCBI PubMed", "callNumber": "", "rights": "", "extra": "PMID: 18302186 \nPMCID: PMC3619977", "tags": [ { "tag": "Animals", "type": 1 }, { "tag": "Benzimidazoles", "type": 1 }, { "tag": "Benzothiazoles", "type": 1 }, { "tag": "DNA, Protozoan", "type": 1 }, { "tag": "Erythrocytes", "type": 1 }, { "tag": "Flow Cytometry", "type": 1 }, { "tag": "Fluorescent Dyes", "type": 1 }, { "tag": "Humans", "type": 1 }, { "tag": "Life Cycle Stages", "type": 1 }, { "tag": "Malaria, Falciparum", "type": 1 }, { "tag": "Plasmodium falciparum", "type": 1 }, { "tag": "Quinolines", "type": 1 }, { "tag": "RNA, Protozoan", "type": 1 }, { "tag": "Ultraviolet Rays", "type": 1 } ], "collections": [ "6DGAWS27" ], "relations": {}, "dateAdded": "2014-03-31T01:31:34Z", "dateModified": "2014-03-31T01:31:34Z" } }, { "key": "BM6JII6M", "version": 30, "library": { "type": "group", "id": 249844, "name": "RBC_Cytometry", "links": { "alternate": { "href": "https://www.zotero.org/groups/rbc_cytometry", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/249844/items/BM6JII6M", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/rbc_cytometry/items/BM6JII6M", "type": "text/html" }, "up": { "href": "https://api.zotero.org/groups/249844/items/XJ9JNJMQ", "type": "application/json" } }, "meta": { "createdByUser": { "id": 46158, "username": "fyshou", "name": "", "links": { "alternate": { "href": "https://www.zotero.org/fyshou", "type": "text/html" } } } }, "data": { "key": "BM6JII6M", "version": 30, "parentItem": "XJ9JNJMQ", "itemType": "attachment", "linkMode": "linked_url", "title": "PubMed entry", "accessDate": "2014-03-31T01:24:36Z", "url": "http://www.ncbi.nlm.nih.gov/pubmed/21296083", "note": "", "contentType": "text/html", "charset": "", "tags": [], "relations": {}, "dateAdded": "2014-03-31T01:24:36Z", "dateModified": "2014-03-31T01:24:36Z" } }, { "key": "XJ9JNJMQ", "version": 30, "library": { "type": "group", "id": 249844, "name": "RBC_Cytometry", "links": { "alternate": { "href": "https://www.zotero.org/groups/rbc_cytometry", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/249844/items/XJ9JNJMQ", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/rbc_cytometry/items/XJ9JNJMQ", "type": "text/html" } }, "meta": { "createdByUser": { "id": 46158, "username": "fyshou", "name": "", "links": { "alternate": { "href": "https://www.zotero.org/fyshou", "type": "text/html" } } }, "creatorSummary": "Grimberg", "parsedDate": "2011-03-31", "numChildren": 1 }, "data": { "key": "XJ9JNJMQ", "version": 30, "itemType": "journalArticle", "title": "Methodology and application of flow cytometry for investigation of human malaria parasites", "creators": [ { "creatorType": "author", "firstName": "Brian T", "lastName": "Grimberg" } ], "abstractNote": "Historically, examinations of the inhibition of malaria parasite growth/invasion, whether using drugs or antibodies, have relied on the use of microscopy or radioactive hypoxanthine uptake. These are considered gold standards for measuring the effectiveness of antimalarial treatments, however, these methods have well known shortcomings. With the advent of flow cytometry coupled with the use of fluorescent DNA stains allowed for increased speed, reproducibility, and qualitative estimates of the effectiveness of antibodies and drugs to limit malaria parasite growth which addresses the challenges of traditional techniques. Because materials and machines available to research facilities are so varied, different methods have been developed to investigate malaria parasites by flow cytometry. This review is intended to serve as a reference guide for advanced users and importantly, as a primer for new users, to support expanded use and improvements to malaria flow cytometry, particularly in endemic countries.", "publicationTitle": "Journal of immunological methods", "publisher": "", "place": "", "date": "Mar 31, 2011", "volume": "367", "issue": "1-2", "section": "", "partNumber": "", "partTitle": "", "pages": "1-16", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "J. Immunol. Methods", "DOI": "10.1016/j.jim.2011.01.015", "citationKey": "", "url": "", "accessDate": "", "PMID": "", "PMCID": "", "ISSN": "1872-7905", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "eng", "libraryCatalog": "NCBI PubMed", "callNumber": "", "rights": "", "extra": "PMID: 21296083 \nPMCID: PMC3071436", "tags": [ { "tag": "Animals", "type": 1 }, { "tag": "Coloring Agents", "type": 1 }, { "tag": "DNA, Protozoan", "type": 1 }, { "tag": "Erythrocytes", "type": 1 }, { "tag": "Flow Cytometry", "type": 1 }, { "tag": "Humans", "type": 1 }, { "tag": "Life Cycle Stages", "type": 1 }, { "tag": "Parasitemia", "type": 1 }, { "tag": "Plasmodium", "type": 1 } ], "collections": [ "6DGAWS27" ], "relations": {}, "dateAdded": "2014-03-31T01:24:36Z", "dateModified": "2014-03-31T01:24:36Z" } }, { "key": "F4E3UARX", "version": 30, "library": { "type": "group", "id": 249844, "name": "RBC_Cytometry", "links": { "alternate": { "href": "https://www.zotero.org/groups/rbc_cytometry", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/249844/items/F4E3UARX", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/rbc_cytometry/items/F4E3UARX", "type": "text/html" }, "up": { "href": "https://api.zotero.org/groups/249844/items/M4G6KM8G", "type": "application/json" } }, "meta": { "createdByUser": { "id": 46158, "username": "fyshou", "name": "", "links": { "alternate": { "href": "https://www.zotero.org/fyshou", "type": "text/html" } } } }, "data": { "key": "F4E3UARX", "version": 30, "parentItem": "M4G6KM8G", "itemType": "attachment", "linkMode": "linked_url", "title": "PubMed entry", "accessDate": "2014-03-31T01:19:11Z", "url": "http://www.ncbi.nlm.nih.gov/pubmed/23835802", "note": "", "contentType": "text/html", "charset": "", "tags": [], "relations": {}, "dateAdded": "2014-03-31T01:19:11Z", "dateModified": "2014-03-31T01:19:11Z" } }, { "key": "M4G6KM8G", "version": 30, "library": { "type": "group", "id": 249844, "name": "RBC_Cytometry", "links": { "alternate": { "href": "https://www.zotero.org/groups/rbc_cytometry", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/249844/items/M4G6KM8G", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/rbc_cytometry/items/M4G6KM8G", "type": "text/html" } }, "meta": { "createdByUser": { "id": 46158, "username": "fyshou", "name": "", "links": { "alternate": { "href": "https://www.zotero.org/fyshou", "type": "text/html" } } }, "creatorSummary": "Shapiro et al.", "parsedDate": "2013-07", "numChildren": 1 }, "data": { "key": "M4G6KM8G", "version": 30, "itemType": "journalArticle", "title": "Cytometry in malaria--a practical replacement for microscopy?", "creators": [ { "creatorType": "author", "firstName": "Howard M", "lastName": "Shapiro" }, { "creatorType": "author", "firstName": "Simon H", "lastName": "Apte" }, { "creatorType": "author", "firstName": "Grace M", "lastName": "Chojnowski" }, { "creatorType": "author", "firstName": "Thomas", "lastName": "Hänscheid" }, { "creatorType": "author", "firstName": "Maria", "lastName": "Rebelo" }, { "creatorType": "author", "firstName": "Brian T", "lastName": "Grimberg" } ], "abstractNote": "Malaria, caused by protozoan Plasmodium parasites, kills ~800,000 people each year. Exact figures are uncertain because presumptive diagnoses are often made without identifying parasites in patients' blood either by microscopy, using Giemsa's century-old stain, or by simpler tests that are ultimately dependent on microscopy for quality control. Microscopy itself relies on trained observers' ability to detect subtle morphological features of parasitized red blood cells, only a few of which may be present on a slide. Quantitative and objective flow cytometric measurements of cellular constituents such as DNA, RNA, and the malaria pigment hemozoin are now useful in research in malaria biology and pharmacology, and can provide more reliable identification of parasite species and developmental stages and better detection of low-density parasitemia than could microscopy. The same measurements can now be implemented in much smaller, simpler, cheaper imaging cytometers, potentially providing a more accurate and precise diagnostic modality.", "publicationTitle": "Current protocols in cytometry / editorial board, J. Paul Robinson, managing editor ... [et al.]", "publisher": "", "place": "", "date": "Jul 2013", "volume": "Chapter 11", "issue": "", "section": "", "partNumber": "", "partTitle": "", "pages": "Unit 11.20", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "Curr Protoc Cytom", "DOI": "10.1002/0471142956.cy1120s65", "citationKey": "", "url": "", "accessDate": "", "PMID": "", "PMCID": "", "ISSN": "1934-9300", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "eng", "libraryCatalog": "NCBI PubMed", "callNumber": "", "rights": "", "extra": "PMID: 23835802", "tags": [ { "tag": "Animals", "type": 1 }, { "tag": "Azure Stains", "type": 1 }, { "tag": "Biomedical Research", "type": 1 }, { "tag": "Flow Cytometry", "type": 1 }, { "tag": "Humans", "type": 1 }, { "tag": "Malaria", "type": 1 }, { "tag": "Microscopy", "type": 1 }, { "tag": "Parasites", "type": 1 } ], "collections": [ "6DGAWS27" ], "relations": {}, "dateAdded": "2014-03-31T01:19:11Z", "dateModified": "2014-03-31T01:19:11Z" } }, { "key": "P8UDQNT7", "version": 29, "library": { "type": "group", "id": 249844, "name": "RBC_Cytometry", "links": { "alternate": { "href": "https://www.zotero.org/groups/rbc_cytometry", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/249844/items/P8UDQNT7", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/rbc_cytometry/items/P8UDQNT7", "type": "text/html" }, "up": { "href": "https://api.zotero.org/groups/249844/items/WK94REBD", "type": "application/json" } }, "meta": { "createdByUser": { "id": 46158, "username": "fyshou", "name": "", "links": { "alternate": { "href": "https://www.zotero.org/fyshou", "type": "text/html" } } } }, "data": { "key": "P8UDQNT7", "version": 29, "parentItem": "WK94REBD", "itemType": "attachment", "linkMode": "linked_url", "title": "PubMed entry", "accessDate": "2014-03-31T01:08:30Z", "url": "http://www.ncbi.nlm.nih.gov/pubmed/22355635", "note": "", "contentType": "text/html", "charset": "", "tags": [], "relations": {}, "dateAdded": "2014-03-31T01:08:30Z", "dateModified": "2014-03-31T01:08:30Z" } }, { "key": "WK94REBD", "version": 29, "library": { "type": "group", "id": 249844, "name": "RBC_Cytometry", "links": { "alternate": { "href": "https://www.zotero.org/groups/rbc_cytometry", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/249844/items/WK94REBD", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/rbc_cytometry/items/WK94REBD", "type": "text/html" } }, "meta": { "createdByUser": { "id": 46158, "username": "fyshou", "name": "", "links": { "alternate": { "href": "https://www.zotero.org/fyshou", "type": "text/html" } } }, "creatorSummary": "Malleret et al.", "parsedDate": "2011", "numChildren": 1 }, "data": { "key": "WK94REBD", "version": 29, "itemType": "journalArticle", "title": "A rapid and robust tri-color flow cytometry assay for monitoring malaria parasite development", "creators": [ { "creatorType": "author", "firstName": "Benoît", "lastName": "Malleret" }, { "creatorType": "author", "firstName": "Carla", "lastName": "Claser" }, { "creatorType": "author", "firstName": "Alice Soh Meoy", "lastName": "Ong" }, { "creatorType": "author", "firstName": "Rossarin", "lastName": "Suwanarusk" }, { "creatorType": "author", "firstName": "Kanlaya", "lastName": "Sriprawat" }, { "creatorType": "author", "firstName": "Shanshan Wu", "lastName": "Howland" }, { "creatorType": "author", "firstName": "Bruce", "lastName": "Russell" }, { "creatorType": "author", "firstName": "Francois", "lastName": "Nosten" }, { "creatorType": "author", "firstName": "Laurent", "lastName": "Rénia" } ], "abstractNote": "Microscopic examination of Giemsa-stained thin blood smears remains the gold standard method used to quantify and stage malaria parasites. However, this technique is tedious, and requires trained microscopists. We have developed a fast and simple flow cytometry method to quantify and stage, various malaria parasites in red blood cells in whole blood or in vitro cultured Plasmodium falciparum. The parasites were stained with dihydroethidium and Hoechst 33342 or SYBR Green I and leukocytes were identified with an antibody against CD45. Depending on the DNA stains used, samples were analyzed using different models of flow cytometers. This protocol, which does not require any washing steps, allows infected red blood cells to be distinguished from leukocytes, as well as allowing non-infected reticulocytes and normocytes to be identified. It also allows assessing the proportion of parasites at different developmental stages. Lastly, we demonstrate how this technique can be applied to antimalarial drug testing.", "publicationTitle": "Scientific reports", "publisher": "", "place": "", "date": "2011", "volume": "1", "issue": "", "section": "", "partNumber": "", "partTitle": "", "pages": "118", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "Sci Rep", "DOI": "10.1038/srep00118", "citationKey": "", "url": "", "accessDate": "", "PMID": "", "PMCID": "", "ISSN": "2045-2322", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "eng", "libraryCatalog": "NCBI PubMed", "callNumber": "", "rights": "", "extra": "PMID: 22355635 \nPMCID: PMC3216599", "tags": [ { "tag": "Animals", "type": 1 }, { "tag": "Antimalarials", "type": 1 }, { "tag": "Drug Evaluation, Preclinical", "type": 1 }, { "tag": "Flow Cytometry", "type": 1 }, { "tag": "Fluorescent Dyes", "type": 1 }, { "tag": "Green Fluorescent Proteins", "type": 1 }, { "tag": "Humans", "type": 1 }, { "tag": "Malaria", "type": 1 }, { "tag": "Mice", "type": 1 }, { "tag": "Mice, Inbred BALB C", "type": 1 }, { "tag": "Mice, Inbred C57BL", "type": 1 }, { "tag": "Parasitemia", "type": 1 }, { "tag": "Plasmodium", "type": 1 }, { "tag": "Plasmodium berghei", "type": 1 }, { "tag": "Plasmodium vivax", "type": 1 }, { "tag": "Plasmodium yoelii", "type": 1 }, { "tag": "Staining and Labeling", "type": 1 } ], "collections": [ "6DGAWS27" ], "relations": {}, "dateAdded": "2014-03-31T01:08:30Z", "dateModified": "2014-03-31T01:08:30Z" } }, { "key": "K2AVZPDE", "version": 28, "library": { "type": "group", "id": 249844, "name": "RBC_Cytometry", "links": { "alternate": { "href": "https://www.zotero.org/groups/rbc_cytometry", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/249844/items/K2AVZPDE", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/rbc_cytometry/items/K2AVZPDE", "type": "text/html" }, "up": { "href": "https://api.zotero.org/groups/249844/items/QN9UCSVN", "type": "application/json" } }, "meta": { "createdByUser": { "id": 46158, "username": "fyshou", "name": "", "links": { "alternate": { "href": "https://www.zotero.org/fyshou", "type": "text/html" } } } }, "data": { "key": "K2AVZPDE", "version": 28, "parentItem": "QN9UCSVN", "itemType": "attachment", "linkMode": "linked_url", "title": "PubMed entry", "accessDate": "2014-03-31T00:49:07Z", "url": "http://www.ncbi.nlm.nih.gov/pubmed/12542437", "note": "", "contentType": "text/html", "charset": "", "tags": [], "relations": {}, "dateAdded": "2014-03-31T00:49:07Z", "dateModified": "2014-03-31T00:49:07Z" } }, { "key": "QN9UCSVN", "version": 28, "library": { "type": "group", "id": 249844, "name": "RBC_Cytometry", "links": { "alternate": { "href": "https://www.zotero.org/groups/rbc_cytometry", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/249844/items/QN9UCSVN", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/rbc_cytometry/items/QN9UCSVN", "type": "text/html" } }, "meta": { "createdByUser": { "id": 46158, "username": "fyshou", "name": "", "links": { "alternate": { "href": "https://www.zotero.org/fyshou", "type": "text/html" } } }, "creatorSummary": "Wang et al.", "parsedDate": "2003-02", "numChildren": 1 }, "data": { "key": "QN9UCSVN", "version": 28, "itemType": "journalArticle", "title": "Development and clinical application of nucleated red blood cell counting and staging on the automated haematology analyser XE-2100", "creators": [ { "creatorType": "author", "firstName": "F-S", "lastName": "Wang" }, { "creatorType": "author", "firstName": "Y", "lastName": "Itose" }, { "creatorType": "author", "firstName": "T", "lastName": "Tsuji" }, { "creatorType": "author", "firstName": "Y", "lastName": "Hamaguchi" }, { "creatorType": "author", "firstName": "K", "lastName": "Hirai" }, { "creatorType": "author", "firstName": "T", "lastName": "Sakata" } ], "abstractNote": "We initially developed a new flow cytometric (FCM) reference method for the enumeration and staging of nucleated red blood cells (NRBC) in 1997 [Wang et al., 1998 (XIth International Symposium on Technological Innovations in Laboratory Haematology, Banff, Canada, 1998); Tsuji et al., 1999 (Cytometry, 1999)]. The method used CD45 antibody and propidium iodide staining to separate NRBCs from other cells. Accuracy and precision were enhanced because larger numbers of cells were counted than was possible with the manual method. We also developed a method for automated NRBC counting on a haematology analyser, the XE-2100 (Wang, 1988). NRBC were separated from other cells using a special lysing buffer and a fluorescent dye. The XE-2100 was found to detect peripheral and cord blood NRBC accurately and precisely when compared with cell morphology or FCM control methods. The FCM NRBC staging method was established through the identification of different NRBC populations following the novel staining and lysing method. To evaluate the method further, we sorted samples containing NRBCs using a FACSort and investigated NRBC staging on the Sysmex XE-2100 based on the cell sorting results. Data were analysed using special software (ida). First, we used the data in various parameter combinations. We then established gates to classify the NRBC populations. Finally, we analysed blood specimens from patients with different types of diseases to explore possible clinical applications.", "publicationTitle": "Clinical and laboratory haematology", "publisher": "", "place": "", "date": "Feb 2003", "volume": "25", "issue": "1", "section": "", "partNumber": "", "partTitle": "", "pages": "17-23", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "Clin Lab Haematol", "DOI": "", "citationKey": "", "url": "", "accessDate": "", "PMID": "", "PMCID": "", "ISSN": "0141-9854", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "eng", "libraryCatalog": "NCBI PubMed", "callNumber": "", "rights": "", "extra": "PMID: 12542437", "tags": [ { "tag": "Blood Cells", "type": 1 }, { "tag": "Cell Separation", "type": 1 }, { "tag": "Erythrocyte Count", "type": 1 }, { "tag": "Fetal Blood", "type": 1 }, { "tag": "Flow Cytometry", "type": 1 }, { "tag": "Hematologic Diseases", "type": 1 }, { "tag": "Humans", "type": 1 }, { "tag": "Reproducibility of Results", "type": 1 } ], "collections": [ "57H3TX72" ], "relations": {}, "dateAdded": "2014-03-31T00:49:07Z", "dateModified": "2014-03-31T00:49:07Z" } }, { "key": "XCNEHII9", "version": 27, "library": { "type": "group", "id": 249844, "name": "RBC_Cytometry", "links": { "alternate": { "href": "https://www.zotero.org/groups/rbc_cytometry", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/249844/items/XCNEHII9", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/rbc_cytometry/items/XCNEHII9", "type": "text/html" }, "up": { "href": "https://api.zotero.org/groups/249844/items/QRSH657Z", "type": "application/json" } }, "meta": { "createdByUser": { "id": 46158, "username": "fyshou", "name": "", "links": { "alternate": { "href": "https://www.zotero.org/fyshou", "type": "text/html" } } } }, "data": { "key": "XCNEHII9", "version": 27, "parentItem": "QRSH657Z", "itemType": "attachment", "linkMode": "linked_url", "title": "PubMed entry", "accessDate": "2014-03-31T00:43:32Z", "url": "http://www.ncbi.nlm.nih.gov/pubmed/10547614", "note": "", "contentType": "text/html", "charset": "", "tags": [], "relations": {}, "dateAdded": "2014-03-31T00:43:32Z", "dateModified": "2014-03-31T00:43:32Z" } }, { "key": "QRSH657Z", "version": 27, "library": { "type": "group", "id": 249844, "name": "RBC_Cytometry", "links": { "alternate": { "href": "https://www.zotero.org/groups/rbc_cytometry", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/249844/items/QRSH657Z", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/rbc_cytometry/items/QRSH657Z", "type": "text/html" } }, "meta": { "createdByUser": { "id": 46158, "username": "fyshou", "name": "", "links": { "alternate": { "href": "https://www.zotero.org/fyshou", "type": "text/html" } } }, "creatorSummary": "Tsuji et al.", "parsedDate": "1999-12-01", "numChildren": 1 }, "data": { "key": "QRSH657Z", "version": 27, "itemType": "journalArticle", "title": "New rapid flow cytometric method for the enumeration of nucleated red blood cells", "creators": [ { "creatorType": "author", "firstName": "T", "lastName": "Tsuji" }, { "creatorType": "author", "firstName": "T", "lastName": "Sakata" }, { "creatorType": "author", "firstName": "Y", "lastName": "Hamaguchi" }, { "creatorType": "author", "firstName": "F s", "lastName": "Wang" }, { "creatorType": "author", "firstName": "B", "lastName": "Houwen" } ], "abstractNote": "BACKGROUND: Nucleated red blood cells (NRBC) in blood specimens compromise the automated white blood cell (WBC) count on most hematology analyzers. This makes it necessary to correct the WBC count by subtracting separately counted NRBC by manual microscopy. In addition, it is clinically important to establish the non-physiological presence of NRBC in blood specimens because of their association with significant hematological and non-hematological disease. Unfortunately, manual microscopic methods lack sensitivity, specificity and reproducibility required for both.\nMETHODS: We have developed a new, rapid flow cytometric method for the detection and enumeration of NRBC, based on two-color staining with anti-CD45-fluorescein-isothiocyanate (CD45-FITC) and propidium iodide (PI). EDTA anticoagulated blood samples are incubated for 30 min with CD45-FITC, followed by 30 sec acid-hypotonic lysis, containing PI and subsequent addition of an alkaline-hypertonic solution. The samples are thus ready for flow cytometric analysis.\nRESULTS: The method typically yields up to four populations, (1) red blood cell (RBC) ghosts, debris, lyse-resistant RBC, reticulocytes and platelets, (2) CD45(+) WBC unstained by PI, (3) CD45(+) WBC stained by PI, and (4) CD45(-)/PI(bright) NRBC. Manual microscopic reference NRBC counts of 25 patient specimens showed excellent correlation with flow cytometric NRBC determinations (y = 0.943x+0. 66; r(2) = 0.982). Performance for precision showed a mean coefficient of variation (CV) for the flow cytometric method of =10%, with a mean CV for manual NRBC counts of 40%.\nCONCLUSIONS: We conclude that this method is suitable for NRBC counting in peripheral blood specimens with improved performance in terms of accuracy, reproducibility when compared to manual microscopic methods.", "publicationTitle": "Cytometry", "publisher": "", "place": "", "date": "Dec 1, 1999", "volume": "37", "issue": "4", "section": "", "partNumber": "", "partTitle": "", "pages": "291-301", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "Cytometry", "DOI": "", "citationKey": "", "url": "", "accessDate": "", "PMID": "", "PMCID": "", "ISSN": "0196-4763", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "eng", "libraryCatalog": "NCBI PubMed", "callNumber": "", "rights": "", "extra": "PMID: 10547614", "tags": [ { "tag": "Adult", "type": 1 }, { "tag": "Antigens, CD45", "type": 1 }, { "tag": "Blood Preservation", "type": 1 }, { "tag": "Erythroblasts", "type": 1 }, { "tag": "Erythrocyte Count", "type": 1 }, { "tag": "Flow Cytometry", "type": 1 }, { "tag": "Fluorescein-5-isothiocyanate", "type": 1 }, { "tag": "Fluorescent Dyes", "type": 1 }, { "tag": "Humans", "type": 1 }, { "tag": "Platelet Aggregation", "type": 1 }, { "tag": "Reproducibility of Results", "type": 1 } ], "collections": [ "57H3TX72" ], "relations": {}, "dateAdded": "2014-03-31T00:43:32Z", "dateModified": "2014-03-31T00:43:32Z" } }, { "key": "HBJ6VZUT", "version": 26, "library": { "type": "group", "id": 249844, "name": "RBC_Cytometry", "links": { "alternate": { "href": "https://www.zotero.org/groups/rbc_cytometry", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/249844/items/HBJ6VZUT", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/rbc_cytometry/items/HBJ6VZUT", "type": "text/html" } }, "meta": { "createdByUser": { "id": 46158, "username": "fyshou", "name": "", "links": { "alternate": { "href": "https://www.zotero.org/fyshou", "type": "text/html" } } }, "creatorSummary": "Won", "parsedDate": "2010-08", "numChildren": 1 }, "data": { "key": "HBJ6VZUT", "version": 26, "itemType": "journalArticle", "title": "[Flow-assisted differential diagnosis of hemolytic anemia with spherocytosis: a case report]", "creators": [ { "creatorType": "author", "firstName": "Dong Il", "lastName": "Won" } ], "abstractNote": "In patients with hemolytic anemia associated with spherocytosis, differential diagnosis has to be made whether the hemolysis is immune-mediated or of non-immune origin. We report a case of hereditary spherocytosis in a 12-yr-old male child, in whom flow-assisted diagnosis was made. In this case, diagnosis was not determined because routine laboratory workups for hereditary spherocytosis yielded discrepant\nRESULTS: positive osmotic fragility test, positive direct antiglobulin test, and normal result in the red cell membrane protein sodium dodecyl succinimide polyacrylamide gel electrophoresis. However, all flow cytometry-based tests, such as osmotic fragility, direct antiglobulin, and eosin 5-maleimide binding test, yielded results compatible with hereditary spherocytosis. Additionally, in family study, the results of eosin 5-maleimide binding test suggested his disease being hereditary. In cases with diagnostic difficulties, flow cytometry may be used as an alternative tool, which can provide additional information in the differential diagnosis of hemolytic anemia with spherocytosis.", "publicationTitle": "The Korean journal of laboratory medicine", "publisher": "", "place": "", "date": "Aug 2010", "volume": "30", "issue": "4", "section": "", "partNumber": "", "partTitle": "", "pages": "339-344", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "Korean J Lab Med", "DOI": "10.3343/kjlm.2010.30.4.339", "citationKey": "", "url": "", "accessDate": "", "PMID": "", "PMCID": "", "ISSN": "1598-6535", "archive": "", "archiveLocation": "", "shortTitle": "[Flow-assisted differential diagnosis of hemolytic anemia with spherocytosis", "language": "kor", "libraryCatalog": "NCBI PubMed", "callNumber": "", "rights": "", "extra": "PMID: 20805704", "tags": [ { "tag": "Anemia, Hemolytic", "type": 1 }, { "tag": "Child", "type": 1 }, { "tag": "Coombs Test", "type": 1 }, { "tag": "Diagnosis, Differential", "type": 1 }, { "tag": "Eosine Yellowish-(YS)", "type": 1 }, { "tag": "Erythrocytes", "type": 1 }, { "tag": "Flow Cytometry", "type": 1 }, { "tag": "Humans", "type": 1 }, { "tag": "Male", "type": 1 }, { "tag": "Osmotic Fragility", "type": 1 }, { "tag": "Spherocytosis, Hereditary", "type": 1 } ], "collections": [ "M8HI482D" ], "relations": {}, "dateAdded": "2014-03-30T23:53:22Z", "dateModified": "2014-03-30T23:53:22Z" } } ]