[ { "key": "R8VCIT8M", "version": 1, "library": { "type": "group", "id": 22361, "name": "TCM", "links": { "alternate": { "href": "https://www.zotero.org/groups/tcm", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/22361/items/R8VCIT8M", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/tcm/items/R8VCIT8M", "type": "text/html" } }, "meta": { "createdByUser": { "id": 119088, "username": "Rayaloha", "name": "", "links": { "alternate": { "href": "https://www.zotero.org/rayaloha", "type": "text/html" } } }, "creatorSummary": "Wu et al.", "parsedDate": "2008-08", "numChildren": 0 }, "data": { "key": "R8VCIT8M", "version": 1, "itemType": "journalArticle", "title": "Analysis of trace elements and macro elements in 13 anti-anoxic traditional Tibetan medicine", "creators": [ { "creatorType": "author", "firstName": "Qi-xun", "lastName": "Wu" }, { "creatorType": "author", "firstName": "Lan", "lastName": "Ji" }, { "creatorType": "author", "firstName": "Jin-liang", "lastName": "Ku" }, { "creatorType": "author", "firstName": "Ping", "lastName": "Song" } ], "abstractNote": "Thirteen kinds of anti-anoxic Tibetan traditional medicine including Hongjingtianjiaonang, Anshensan, Wuweishiliusan, Zhangsongbaweichenxiangsan, Shiliuweidujuansan, Ershiwuweiyuganziwan, Ershiwuweizhuhuangjiaonang, Shiwuweilongdanwan, Mijueqingliangsan, Qishiweizhenzhuwan, Bawei-chenxiangsan, Liuweidingxiangsan and Qiweiputansan were digested with HNO3-HClO4 mixed acid. Fourteen trace elements and macro elements in the 13 kinds of anti-anoxic Tibetan traditional medicine, such as silver, aluminium, cadmium, cobalt, chromium, copper, ferrum, hydrargyrum, magnesium, manganese, nickel, lead, stannum and zinc were determined by flame atomic absorption spectrophotometry (FAAS). The results showed that there were comparatively rich trace elements and macro elements in kinds of anti-anoxic Tibetan traditional Medicine. The 13 Anti-anoxic Tibetan traditional medicine contains higher amount of ferrum, magnesium, copper, chromium, zinc and manganese than other elements. This provided useful data for discussing the relationship between trace elements and macro elements and anti-anoxic activities in Tibetan traditional medicine.", "publicationTitle": "Guang Pu Xue Yu Guang Pu Fen Xi = Guang Pu", "publisher": "", "place": "", "date": "Aug 2008", "volume": "28", "issue": "8", "section": "", "partNumber": "", "partTitle": "", "pages": "1938-1941", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "Guang Pu Xue Yu Guang Pu Fen Xi", "DOI": "", "citationKey": "", "url": "http://www.ncbi.nlm.nih.gov/pubmed/18975838", "accessDate": "2010-07-27T06:42:44Z", "PMID": "", "PMCID": "", "ISSN": "1000-0593", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "", "libraryCatalog": "NCBI PubMed", "callNumber": "", "rights": "", "extra": "PMID: 18975838", "tags": [ { "tag": "Anoxia", "type": 1 }, { "tag": "Drugs, Chinese Herbal", "type": 1 }, { "tag": "Humans", "type": 1 }, { "tag": "Medicine, Tibetan Traditional", "type": 1 }, { "tag": "Oxygen Consumption", "type": 1 }, { "tag": "Principal Component Analysis", "type": 1 }, { "tag": "Spectrophotometry, Atomic", "type": 1 }, { "tag": "Trace Elements", "type": 1 } ], "collections": [ "UITAV6DP" ], "relations": {}, "dateAdded": "2010-07-27T06:42:44Z", "dateModified": "2011-03-08T07:38:54Z" } }, { "key": "AG3H9DGN", "version": 1, "library": { "type": "group", "id": 22361, "name": "TCM", "links": { "alternate": { "href": "https://www.zotero.org/groups/tcm", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/22361/items/AG3H9DGN", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/tcm/items/AG3H9DGN", "type": "text/html" } }, "meta": { "createdByUser": { "id": 119088, "username": "Rayaloha", "name": "", "links": { "alternate": { "href": "https://www.zotero.org/rayaloha", "type": "text/html" } } }, "creatorSummary": "Qiu et al.", "parsedDate": "2007-04-11", "numChildren": 0 }, "data": { "key": "AG3H9DGN", "version": 1, "itemType": "journalArticle", "title": "Study of traditional Chinese medicine volatile oils from different geographical origins by comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry (GC × GC-TOFMS) in combination with multivariate analysis", "creators": [ { "creatorType": "author", "firstName": "Yaqiong", "lastName": "Qiu" }, { "creatorType": "author", "firstName": "Xin", "lastName": "Lu" }, { "creatorType": "author", "firstName": "Tao", "lastName": "Pang" }, { "creatorType": "author", "firstName": "Shukui", "lastName": "Zhu" }, { "creatorType": "author", "firstName": "Hongwei", "lastName": "Kong" }, { "creatorType": "author", "firstName": "Guowang", "lastName": "Xu" } ], "abstractNote": "Chemical constitutes of volatile oil in the rhizomes and radixes of Notopterygium incisum Ting ex H.T. chang (Qianghuo in Chinese) from different regions were investigated using comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry (GC × GC-TOFMS) and GC × GC-flame ionization detector (FID). A total of 769 compounds were tentatively identified and quantified in a typical sample from Sichuan province, a producing area of the Genuine Medicinal Materials. An obvious group-type separation was observed in the GC × GC-TOFMS chromatogram. Identification and quantitative results showed that Qianghuo from Sichuan province has some significant differences in the chemical composition from other geographical origin of herbs, especially in monoterpenes and oxygenated sesquiterpenes. The data of all individual peaks collected by GC × GC-FID were processed using a principal component analysis (PCA) method to classify the samples from different regions, find and identify the marker compounds that lead to the differentiation. The abundances of monoterpenes and oxygenated sesquiterpenes were responsible for the differentiation, which is in good agreement with the group quantitative results of GC × GC analysis.", "publicationTitle": "Journal of Pharmaceutical and Biomedical Analysis", "publisher": "", "place": "", "date": "April 11, 2007", "volume": "43", "issue": "5", "section": "", "partNumber": "", "partTitle": "", "pages": "1721-1727", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "", "DOI": "10.1016/j.jpba.2007.01.013", "citationKey": "", "url": "http://www.sciencedirect.com/science/article/B6TGX-4MV0M8R-1/2/d43f0dcfe72d1e3d192f0ba39583bd7d", "accessDate": "2011-03-03T07:31:04Z", "PMID": "", "PMCID": "", "ISSN": "0731-7085", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "", "libraryCatalog": "ScienceDirect", "callNumber": "", "rights": "", "extra": "", "tags": [ { "tag": "GC × GC-TOFMS", "type": 1 }, { "tag": "Notopterygium incisum Ting ex H.T. chang", "type": 1 }, { "tag": "Principal Component Analysis", "type": 1 }, { "tag": "Qianghuo", "type": 1 }, { "tag": "Traditional Chinese medicine", "type": 1 } ], "collections": [], "relations": { "dc:relation": "http://zotero.org/groups/22361/items/JMSFDTAB" }, "dateAdded": "2011-03-03T07:31:08Z", "dateModified": "2011-03-07T13:50:51Z" } }, { "key": "JMSFDTAB", "version": 1, "library": { "type": "group", "id": 22361, "name": "TCM", "links": { "alternate": { "href": "https://www.zotero.org/groups/tcm", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/22361/items/JMSFDTAB", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/tcm/items/JMSFDTAB", "type": "text/html" } }, "meta": { "createdByUser": { "id": 119088, "username": "Rayaloha", "name": "", "links": { "alternate": { "href": "https://www.zotero.org/rayaloha", "type": "text/html" } } }, "creatorSummary": "Wu et al.", "parsedDate": "2004-04-23", "numChildren": 0 }, "data": { "key": "JMSFDTAB", "version": 1, "itemType": "journalArticle", "title": "Application of comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry in the analysis of volatile oil of traditional Chinese medicines", "creators": [ { "creatorType": "author", "firstName": "Jianfang", "lastName": "Wu" }, { "creatorType": "author", "firstName": "Xin", "lastName": "Lu" }, { "creatorType": "author", "firstName": "Wanying", "lastName": "Tang" }, { "creatorType": "author", "firstName": "Hongwei", "lastName": "Kong" }, { "creatorType": "author", "firstName": "Shenfan", "lastName": "Zhou" }, { "creatorType": "author", "firstName": "Guowang", "lastName": "Xu" } ], "abstractNote": "This paper reports comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry (GC × GC-TOF MS) analysis of Pogostemon cablin Benth (Cablin Patchouli) volatile oil. The suitable column system and operation conditions were chosen on the basis of the properties of composition of the volatile oil. One-dimensional gas chromatography (1D-GC) and GC × GC, GC-MS and GC × GC-TOF MS were compared under appropriate conditions, and the enhanced sensitivity and superior resolution of GC × GC were demonstrated. 394 components were tentatively identified by GC × GC-TOF MS.", "publicationTitle": "Journal of Chromatography A", "publisher": "", "place": "", "date": "April 23, 2004", "volume": "1034", "issue": "1-2", "section": "", "partNumber": "", "partTitle": "", "pages": "199-205", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "", "DOI": "10.1016/j.chroma.2004.02.028", "citationKey": "", "url": "http://www.sciencedirect.com/science/article/B6TG8-4BWYRJM-G/2/d6314f56f7dc8b3c1144e134324e8dfe", "accessDate": "2011-03-07T13:49:56Z", "PMID": "", "PMCID": "", "ISSN": "0021-9673", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "", "libraryCatalog": "ScienceDirect", "callNumber": "", "rights": "", "extra": "", "tags": [ { "tag": "Chinese medicines", "type": 1 }, { "tag": "Essential oils", "type": 1 }, { "tag": "Gas chromatography, comprehensive two-dimensional", "type": 1 }, { "tag": "Pogostemon cablin Benth", "type": 1 } ], "collections": [], "relations": { "dc:relation": "http://zotero.org/groups/22361/items/AG3H9DGN" }, "dateAdded": "2011-03-07T13:49:56Z", "dateModified": "2011-03-07T13:49:56Z" } }, { "key": "EWUA2PFN", "version": 1, "library": { "type": "group", "id": 22361, "name": "TCM", "links": { "alternate": { "href": "https://www.zotero.org/groups/tcm", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/22361/items/EWUA2PFN", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/tcm/items/EWUA2PFN", "type": "text/html" } }, "meta": { "createdByUser": { "id": 119088, "username": "Rayaloha", "name": "", "links": { "alternate": { "href": "https://www.zotero.org/rayaloha", "type": "text/html" } } }, "creatorSummary": "Yang et al.", "parsedDate": "2008-10", "numChildren": 0 }, "data": { "key": "EWUA2PFN", "version": 1, "itemType": "journalArticle", "title": "Principal component and cluster analysis of trace elements in Chinese herb Salvia miltiorrhiza and its relative species", "creators": [ { "creatorType": "author", "firstName": "Zai-jun", "lastName": "Yang" }, { "creatorType": "author", "firstName": "Li", "lastName": "Zhang" }, { "creatorType": "author", "firstName": "Rui-wu", "lastName": "Yang" }, { "creatorType": "author", "firstName": "Chun-bang", "lastName": "Ding" }, { "creatorType": "author", "firstName": "Yong-hong", "lastName": "Zhou" }, { "creatorType": "author", "firstName": "De-guang", "lastName": "Wan" } ], "abstractNote": "The contents of trace elements, including copper, zinc, iron, magnesium, calcium, chromium, lead, molybdenum, manganese, and cadmium in Chinese traditional herb S. miltiorrhiza and its relative species such as S. miltiorrhiza f. alba, S. evansiana, S. yunnanensis, S. przewarlskii, S. cavaleriei, S. cavaleriei var. simplicifolia, S. roborowskii, S. omeiana, S. tricuspis, S. brevilabra and S. cynica were determined by atomic absorption spectroscopy. The principal components analysis of SPSS was applied to the study of characteristic elements in S. miltiorrhiza and its relative species. Three principal components which accounted for 79.3% of total variance were extracted from the original data. The first factor accounted for 49.6% of the total variance, which means that iron, manganese, copper, zinc, cadmium and lead are the characteristic elements in S. miltiorrhiza and its relative species. The results of cluster analysis show that the samples could cluster reasonably into two groups. The samples of S. miltiorrhiza from different regions were classified into one group except S. miltiorrhiza from Zhejiang and S. miltiorrhiza f. alba. The other Salvia species were classified into another group except S. cavaleriei. The S. miltiorrhiza and other Salvia species can be distinguished by this method, whose accuracy of classification is 90%. The cluster analysis based on the contents of trace element in S. miltiorrhiza and its relative species provided a quick, accurate and simple method for authentication of herb Salvia miltiorrhiza.", "publicationTitle": "Guang Pu Xue Yu Guang Pu Fen Xi = Guang Pu", "publisher": "", "place": "", "date": "Oct 2008", "volume": "28", "issue": "10", "section": "", "partNumber": "", "partTitle": "", "pages": "2441-2445", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "Guang Pu Xue Yu Guang Pu Fen Xi", "DOI": "", "citationKey": "", "url": "http://www.ncbi.nlm.nih.gov/pubmed/19123426", "accessDate": "2010-07-27T06:33:54Z", "PMID": "", "PMCID": "", "ISSN": "1000-0593", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "", "libraryCatalog": "NCBI PubMed", "callNumber": "", "rights": "", "extra": "PMID: 19123426", "tags": [ { "tag": "Cadmium", "type": 1 }, { "tag": "Calcium", "type": 1 }, { "tag": "Chromium", "type": 1 }, { "tag": "Cluster Analysis", "type": 1 }, { "tag": "Copper", "type": 1 }, { "tag": "Iron", "type": 1 }, { "tag": "Magnesium", "type": 1 }, { "tag": "Manganese", "type": 1 }, { "tag": "Principal Component Analysis", "type": 1 }, { "tag": "Salvia miltiorrhiza", "type": 1 }, { "tag": "Spectrophotometry, Atomic", "type": 1 }, { "tag": "Trace Elements", "type": 1 }, { "tag": "Zinc", "type": 1 } ], "collections": [ "S5WJHTTJ" ], "relations": { "owl:sameAs": "http://zotero.org/groups/21547/items/DTUP4RFX" }, "dateAdded": "2010-07-27T06:38:33Z", "dateModified": "2011-03-07T05:26:53Z" } }, { "key": "VEIWEZBJ", "version": 1, "library": { "type": "group", "id": 22361, "name": "TCM", "links": { "alternate": { "href": "https://www.zotero.org/groups/tcm", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/22361/items/VEIWEZBJ", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/tcm/items/VEIWEZBJ", "type": "text/html" } }, "meta": { "createdByUser": { "id": 119088, "username": "Rayaloha", "name": "", "links": { "alternate": { "href": "https://www.zotero.org/rayaloha", "type": "text/html" } } }, "creatorSummary": "He et al.", "parsedDate": "2008-01", "numChildren": 0 }, "data": { "key": "VEIWEZBJ", "version": 1, "itemType": "journalArticle", "title": "Principal factor analysis of symptoms of rheumatoid arthritis and their correlations with efficacy of traditional Chinese medicine and Western medicine", "creators": [ { "creatorType": "author", "firstName": "Yi-Ting", "lastName": "He" }, { "creatorType": "author", "firstName": "Qing-Lin", "lastName": "Zha" }, { "creatorType": "author", "firstName": "Jian-Ping", "lastName": "Yu" }, { "creatorType": "author", "firstName": "Yong", "lastName": "Tan" }, { "creatorType": "author", "firstName": "Cheng", "lastName": "Lu" }, { "creatorType": "author", "firstName": "Ai-Ping", "lastName": "Lu" } ], "abstractNote": "OBJECTIVE: To explore the correlations between symptom-based principal factors of rheumatoid arthritis (RA) and the effect of traditional Chinese medicine or Western medicine on RA after factor analysis of RA symptoms. METHODS: Four hundred and thirteen RA patients from 9 clinical centers were included in the clinical trial. They were randomly divided into Western medicine (WM) treated group with 204 cases and traditional Chinese medicine (CM) treated group with 209 cases. A complete physical examination and 18 common clinical manifestations were recorded before the randomization and after the treatment. The WM therapy included voltaren extended action tablet, methotrexate and sulfasalazine. The CM therapy included glucosidorum Tripterygll totorum tablet and Yishen Juanbi Tablet. The American College of Rheumatology 20 (ACR20) was used for efficacy evaluation. All data were analyzed on SAS 8.2 statistical package. Eighteen symptoms in the RA patients were analyzed by factor analysis and the relationships between the factors and effects were analyzed with Chi-Square test. RESULTS: Four principal factors were obtained from the analysis results of the 18 symptoms. The factors could represent the symptoms related to joints, cold-syndrome, deficiency syndrome and heat-syndrome in traditional Chinese medicine (TCM), respectively. The effect of WM therapy was better than CM therapy. After 12 weeks of treatment, the effect of CM on patients without deficiency-syndrome was better than the patients with deficiency-syndrome. After 24 weeks of treatment, WM therapy showed better effect on patients with cold syndrome than patients without cold syndrome. CONCLUSION: The results based on the factor analysis of RA symptoms are similar to the results of syndrome differentiation of TCM, and the factor-related different categories of symptoms are associated with the curative effect, thus further research on the symptoms is necessary.", "publicationTitle": "Zhong Xi Yi Jie He Xue Bao = Journal of Chinese Integrative Medicine", "publisher": "", "place": "", "date": "Jan 2008", "volume": "6", "issue": "1", "section": "", "partNumber": "", "partTitle": "", "pages": "32-36", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "Zhong Xi Yi Jie He Xue Bao", "DOI": "", "citationKey": "", "url": "http://www.ncbi.nlm.nih.gov/pubmed/18184543", "accessDate": "2010-07-27T06:49:19Z", "PMID": "", "PMCID": "", "ISSN": "1672-1977", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "", "libraryCatalog": "NCBI PubMed", "callNumber": "", "rights": "", "extra": "PMID: 18184543", "tags": [ { "tag": "Adult", "type": 1 }, { "tag": "Anti-Inflammatory Agents, Non-Steroidal", "type": 1 }, { "tag": "Arthritis, Rheumatoid", "type": 1 }, { "tag": "Diagnosis, Differential", "type": 1 }, { "tag": "Drugs, Chinese Herbal", "type": 1 }, { "tag": "Female", "type": 1 }, { "tag": "Humans", "type": 1 }, { "tag": "Male", "type": 1 }, { "tag": "Medicine, Chinese Traditional", "type": 1 }, { "tag": "Middle Aged", "type": 1 }, { "tag": "Phytotherapy", "type": 1 }, { "tag": "Principal Component Analysis", "type": 1 }, { "tag": "Treatment Outcome", "type": 1 } ], "collections": [ "S5WJHTTJ" ], "relations": {}, "dateAdded": "2010-07-27T06:49:19Z", "dateModified": "2011-03-07T05:26:42Z" } }, { "key": "4A2J4F5T", "version": 1, "library": { "type": "group", "id": 22361, "name": "TCM", "links": { "alternate": { "href": "https://www.zotero.org/groups/tcm", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/22361/items/4A2J4F5T", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/tcm/items/4A2J4F5T", "type": "text/html" } }, "meta": { "createdByUser": { "id": 119088, "username": "Rayaloha", "name": "", "links": { "alternate": { "href": "https://www.zotero.org/rayaloha", "type": "text/html" } } }, "creatorSummary": "Wang et al.", "parsedDate": "2010-11-02", "numChildren": 0 }, "data": { "key": "4A2J4F5T", "version": 1, "itemType": "journalArticle", "title": "Thyroxine and reserpine-induced changes in metabolic profiles of rat urine and the therapeutic effect of Liu Wei Di Huang Wan detected by UPLC-HDMS", "creators": [ { "creatorType": "author", "firstName": "Ping", "lastName": "Wang" }, { "creatorType": "author", "firstName": "Hui", "lastName": "Sun" }, { "creatorType": "author", "firstName": "Haitao", "lastName": "Lv" }, { "creatorType": "author", "firstName": "Wenjun", "lastName": "Sun" }, { "creatorType": "author", "firstName": "Ye", "lastName": "Yuan" }, { "creatorType": "author", "firstName": "Ying", "lastName": "Han" }, { "creatorType": "author", "firstName": "Dongwu", "lastName": "Wang" }, { "creatorType": "author", "firstName": "Aihua", "lastName": "Zhang" }, { "creatorType": "author", "firstName": "Xijun", "lastName": "Wang" } ], "abstractNote": "The promise of metabonomics, a new \"omics\" technique, to validate Chinese medicines and the compatibility of Chinese formulas has been appreciated. The present study was undertaken to explore the excretion pattern of low molecular mass metabolites in the male Wistar-derived rat model of kidney yin deficiency induced with thyroxine and reserpine as well as the therapeutic effect of Liu Wei Di Huang Wan (LW) and its separated prescriptions, a classic traditional Chinese medicine formula for treating kidney yin deficiency in China. The study utilized ultra-performance liquid chromatography/electrospray ionization synapt high definition mass spectrometry (UPLC/ESI-SYNAPT-HDMS) in both negative and positive electrospray ionization (ESI). At the same time, blood biochemistry was examined to identify specific changes in the kidney yin deficiency. Distinct changes in the pattern of metabolites, as a result of daily administration of thyroxine and reserpine, were observed by UPLC-HDMS combined with a principal component analysis (PCA). The changes in metabolic profiling were restored to their baseline values after treatment with LW according to the PCA score plots. Altogether, the current metabonomic approach based on UPLC-HDMS and orthogonal projection to latent structures discriminate analysis (OPLS-DA) indicated 20 ions (14 in the negative mode, 8 in the positive mode, and 2 in both) as \"differentiating metabolites\".", "publicationTitle": "Journal of Pharmaceutical and Biomedical Analysis", "publisher": "", "place": "", "date": "Nov 2, 2010", "volume": "53", "issue": "3", "section": "", "partNumber": "", "partTitle": "", "pages": "631-645", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "J Pharm Biomed Anal", "DOI": "10.1016/j.jpba.2010.04.032", "citationKey": "", "url": "http://www.ncbi.nlm.nih.gov/pubmed/20605388", "accessDate": "2010-07-27T08:36:21Z", "PMID": "", "PMCID": "", "ISSN": "1873-264X", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "", "libraryCatalog": "NCBI PubMed", "callNumber": "", "rights": "", "extra": "PMID: 20605388", "tags": [], "collections": [], "relations": {}, "dateAdded": "2010-07-27T08:36:21Z", "dateModified": "2010-07-27T08:36:21Z" } }, { "key": "4ZPRRZ34", "version": 1, "library": { "type": "group", "id": 22361, "name": "TCM", "links": { "alternate": { "href": "https://www.zotero.org/groups/tcm", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/22361/items/4ZPRRZ34", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/tcm/items/4ZPRRZ34", "type": "text/html" } }, "meta": { "createdByUser": { "id": 119088, "username": "Rayaloha", "name": "", "links": { "alternate": { "href": "https://www.zotero.org/rayaloha", "type": "text/html" } } }, "creatorSummary": "Chen et al.", "parsedDate": "2008-12-17", "numChildren": 0 }, "data": { "key": "4ZPRRZ34", "version": 1, "itemType": "journalArticle", "title": "Rapid characterization of plant mutants with an altered ion-profile: a case study using Lotus japonicus", "creators": [ { "creatorType": "author", "firstName": "Zheng", "lastName": "Chen" }, { "creatorType": "author", "firstName": "Toshihiro", "lastName": "Watanabe" }, { "creatorType": "author", "firstName": "Takuro", "lastName": "Shinano" }, { "creatorType": "author", "firstName": "Keiki", "lastName": "Okazaki" }, { "creatorType": "author", "firstName": "Mitsuru", "lastName": "Osaki" } ], "abstractNote": "* Legumes are second only to cereals in their importance to humans, and study of their functional genomics of nutrition and other trace elements is crucial for agricultural production and food fortification. We describe here an ionomic screening experiment carried out to investigate the accumulation of 15 elements in shoots of mutants of Lotus japonicus, a good genetic tool for legume study. * Approximately 2000 mutagenized M2 plants were cultivated in a novel low-cost high-throughput system and their elemental profiles were determined by inductively coupled plasma mass spectroscopy (ICP-MS). * After triple-checking the element concentrations in M2 or M3 plant shoots, 31 mutants with altered elemental profiles were identified. Surprisingly, the number of genes regulating essential elements was similar to the number regulating nonessential elements. Magnesium (Mg) and nickel (Ni) were correlated in a number of mutants. Further investigation suggested that phosphorus (P) and cobalt (Co) might be involved in the ion homeostasis network of Mg and Ni. * The results suggested that the pathways for element uptake or translocation were highly linked through the ion transport-related genes. Ionomics proved to be a powerful functional genomics tool for determining genes related to ion homeostasis in this study.", "publicationTitle": "The New Phytologist", "publisher": "", "place": "", "date": "Dec 17, 2008", "volume": "", "issue": "", "section": "", "partNumber": "", "partTitle": "", "pages": "", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "New Phytol", "DOI": "10.1111/j.1469-8137.2008.02730.x", "citationKey": "", "url": "http://www.ncbi.nlm.nih.gov/pubmed/19140942", "accessDate": "2010-02-24T02:33:06Z", "PMID": "", "PMCID": "", "ISSN": "1469-8137", "archive": "", "archiveLocation": "", "shortTitle": "Rapid characterization of plant mutants with an altered ion-profile", "language": "", "libraryCatalog": "NCBI PubMed", "callNumber": "", "rights": "", "extra": "PMID: 19140942", "tags": [], "collections": [ "UITAV6DP" ], "relations": { "owl:sameAs": "http://zotero.org/groups/13326/items/DBIP2W3S" }, "dateAdded": "2010-07-27T06:43:56Z", "dateModified": "2010-07-27T06:43:56Z" } } ]