[ { "key": "2AQZLAYJ", "version": 106, "library": { "type": "group", "id": 159246, "name": "NMR group", "links": { "alternate": { "href": "https://www.zotero.org/groups/nmr_group", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/159246/items/2AQZLAYJ", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/nmr_group/items/2AQZLAYJ", "type": "text/html" } }, "meta": { "createdByUser": { "id": 283300, "username": "glewin", "name": "Gary Lewin", "links": { "alternate": { "href": "https://www.zotero.org/glewin", "type": "text/html" } } }, "creatorSummary": "Holmes et al.", "parsedDate": "2011-03-17", "numChildren": 0 }, "data": { "key": "2AQZLAYJ", "version": 106, "itemType": "journalArticle", "title": "Social and hormonal triggers of neural plasticity in naked mole-rats", "creators": [ { "creatorType": "author", "firstName": "Melissa M.", "lastName": "Holmes" }, { "creatorType": "author", "firstName": "Marianne L.", "lastName": "Seney" }, { "creatorType": "author", "firstName": "Bruce D.", "lastName": "Goldman" }, { "creatorType": "author", "firstName": "Nancy G.", "lastName": "Forger" } ], "abstractNote": "Naked mole-rats are eusocial rodents that live in large social groups with a strict reproductive hierarchy. In each colony only a few individuals breed; all others are non-reproductive subordinates. We previously showed that breeders have increased volume of several brain regions linked to reproduction: the paraventricular nucleus of the hypothalamus (PVN), the principal nucleus of the bed nucleus of the stria terminalis (BSTp), and the medial amygdala (MeA). Breeders also have more large motoneurons in Onuf's nucleus (ON) in the spinal cord, a cell group innervating perineal muscles that attach to the genitalia. Here, we sought to determine triggers for the neural changes seen in breeders. Specifically, we compared four groups of animals: subordinates, paired animals that did not reproduce, gonadally intact breeders, and gonadectomized breeders. We find that pairing alone is sufficient to cause breeder-like changes in volume of the PVN and cell size distribution in ON. In contrast, increases in BSTp volume were seen only in animals that actually reproduced. Those changes that were seen in successful breeders appear to be independent of gonadal steroids because long-term gonadectomy did not reverse the breeder-like neural changes in the PVN, BSTp or ON, although a trend for gonadectomized animals having larger MeA volumes was detected. Thus, neural changes associated with breeding status in naked mole-rats may be triggered by different aspects of the social and reproductive environment; once changes occur they are largely independent of gonadal hormones and may be permanent.", "publicationTitle": "Behavioural Brain Research", "publisher": "", "place": "", "date": "2011-03-17", "volume": "218", "issue": "1", "section": "", "partNumber": "", "partTitle": "", "pages": "234-239", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "Behavioural Brain Research", "DOI": "10.1016/j.bbr.2010.11.056", "citationKey": "", "url": "https://www.sciencedirect.com/science/article/pii/S0166432810007813", "accessDate": "2025-08-31T16:16:15Z", "PMID": "", "PMCID": "", "ISSN": "0166-4328", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "", "libraryCatalog": "ScienceDirect", "callNumber": "", "rights": "", "extra": "", "tags": [ { "tag": "Bed nucleus of the stria terminalis", "type": 1 }, { "tag": "Naked mole-rat", "type": 1 }, { "tag": "Neuroplasticity", "type": 1 }, { "tag": "Onuf's nucleus", "type": 1 }, { "tag": "Paraventricular nucleus", "type": 1 }, { "tag": "Social status", "type": 1 } ], "collections": [], "relations": {}, "dateAdded": "2025-08-31T16:16:15Z", "dateModified": "2025-08-31T16:16:15Z" } }, { "key": "C7B7RQF2", "version": 105, "library": { "type": "group", "id": 159246, "name": "NMR group", "links": { "alternate": { "href": "https://www.zotero.org/groups/nmr_group", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/159246/items/C7B7RQF2", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/nmr_group/items/C7B7RQF2", "type": "text/html" }, "up": { "href": "https://api.zotero.org/groups/159246/items/XUKW9Y2W", "type": "application/json" } }, "meta": { "createdByUser": { "id": 283300, "username": "glewin", "name": "Gary Lewin", "links": { "alternate": { "href": "https://www.zotero.org/glewin", "type": "text/html" } } } }, "data": { "key": "C7B7RQF2", "version": 105, "parentItem": "XUKW9Y2W", "itemType": "attachment", "linkMode": "linked_url", "title": "PubMed entry", "accessDate": "2025-08-31T11:16:49Z", "url": "http://www.ncbi.nlm.nih.gov/pubmed/29771243", "note": "", "contentType": "text/html", "charset": "", "tags": [], "relations": {}, "dateAdded": "2025-08-31T11:16:49Z", "dateModified": "2025-08-31T11:16:49Z" } }, { "key": "XUKW9Y2W", "version": 105, "library": { "type": "group", "id": 159246, "name": "NMR group", "links": { "alternate": { "href": "https://www.zotero.org/groups/nmr_group", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/159246/items/XUKW9Y2W", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/nmr_group/items/XUKW9Y2W", "type": "text/html" } }, "meta": { "createdByUser": { "id": 283300, "username": "glewin", "name": "Gary Lewin", "links": { "alternate": { "href": "https://www.zotero.org/glewin", "type": "text/html" } } }, "creatorSummary": "Küppers et al.", "parsedDate": "2018-06-20", "numChildren": 1 }, "data": { "key": "XUKW9Y2W", "version": 105, "itemType": "journalArticle", "title": "Breath volatile organic compounds of lung transplant recipients with and without chronic lung allograft dysfunction", "creators": [ { "creatorType": "author", "firstName": "L.", "lastName": "Küppers" }, { "creatorType": "author", "firstName": "O.", "lastName": "Holz" }, { "creatorType": "author", "firstName": "S.", "lastName": "Schuchardt" }, { "creatorType": "author", "firstName": "J.", "lastName": "Gottlieb" }, { "creatorType": "author", "firstName": "J.", "lastName": "Fuge" }, { "creatorType": "author", "firstName": "M.", "lastName": "Greer" }, { "creatorType": "author", "firstName": "J. M.", "lastName": "Hohlfeld" } ], "abstractNote": "INTRODUCTION: Chronic lung allograft dysfunction with its clinical correlative of bronchiolitis obliterans syndrome (BOS) remains the major limiting factor for long-term graft survival. Currently there are no established methods for the early diagnosis or prediction of BOS. To assess the feasibility of breath collection as a non-invasive tool and the potential of breath volatile organic compounds (VOC) for the early detection of BOS, we compared the breath VOC composition between transplant patients without and different stages of BOS.\nMETHODS: 75 outpatients (25 BOS stage 0, 25 BOS stage 1 + 2, 25 BOS stage 3) after bilateral lung transplantation were included. Exclusion criteria were active smoking, oxygen therapy and acute infection. Patients inhaled room air through a VOC and sterile filter and exhaled into an aluminum reservoir tube. Breath was loaded directly onto Tenax® TA adsorption tubes and was subsequently analyzed by gas-chromatography/mass-spectrometry.\nRESULTS: The three groups were age and gender matched, but differed with respect to time since transplantation, the spectrum of underlying disease, and treatment regimes. Relative to patients without BOS, BOS stage 3 patients showed a larger number of different VOCs, and more pronounced differences in the level of VOCs as compared to BOS stage 1 + 2 patients. Logistic regression analysis found no differences between controls and BOS 1 + 2, but four VOCs (heptane, isopropyl-myristate, ethyl-acetate, ionone) with a significant contribution to the discrimination between controls and BOS stage 3. A combination of these four VOCs separated these groups with an area under the curve of 0.87.\nCONCLUSION: Breath sample collection using our reservoir sampler in the clinical environment was feasible. Our results suggest that breath VOCs can discriminate severe BOS. However, convincing evidence for VOCs with a potential to detect early onset BOS is lacking.", "publicationTitle": "Journal of Breath Research", "publisher": "", "place": "", "date": "2018-06-20", "volume": "12", "issue": "3", "section": "", "partNumber": "", "partTitle": "", "pages": "036023", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "J Breath Res", "DOI": "10.1088/1752-7163/aac5af", "citationKey": "", "url": "", "accessDate": "", "PMID": "", "PMCID": "", "ISSN": "1752-7163", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "eng", "libraryCatalog": "PubMed", "callNumber": "", "rights": "", "extra": "PMID: 29771243", "tags": [ { "tag": "Allografts", "type": 1 }, { "tag": "Breath Tests", "type": 1 }, { "tag": "Confounding Factors, Epidemiologic", "type": 1 }, { "tag": "Female", "type": 1 }, { "tag": "Humans", "type": 1 }, { "tag": "Lung Transplantation", "type": 1 }, { "tag": "Male", "type": 1 }, { "tag": "Middle Aged", "type": 1 }, { "tag": "Multivariate Analysis", "type": 1 }, { "tag": "Smoking", "type": 1 }, { "tag": "Transplant Recipients", "type": 1 }, { "tag": "Volatile Organic Compounds", "type": 1 } ], "collections": [], "relations": {}, "dateAdded": "2025-08-31T11:16:48Z", "dateModified": "2025-08-31T11:16:48Z" } }, { "key": "W7J7QEE8", "version": 104, "library": { "type": "group", "id": 159246, "name": "NMR group", "links": { "alternate": { "href": "https://www.zotero.org/groups/nmr_group", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/159246/items/W7J7QEE8", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/nmr_group/items/W7J7QEE8", "type": "text/html" }, "up": { "href": "https://api.zotero.org/groups/159246/items/AYIGVL9R", "type": "application/json" } }, "meta": { "createdByUser": { "id": 283300, "username": "glewin", "name": "Gary Lewin", "links": { "alternate": { "href": "https://www.zotero.org/glewin", "type": "text/html" } } } }, "data": { "key": "W7J7QEE8", "version": 104, "parentItem": "AYIGVL9R", "itemType": "attachment", "linkMode": "linked_url", "title": "PubMed entry", "accessDate": "2025-08-20T14:32:39Z", "url": "http://www.ncbi.nlm.nih.gov/pubmed/38281330", "note": "", "contentType": "text/html", "charset": "", "tags": [], "relations": {}, "dateAdded": "2025-08-20T14:32:39Z", "dateModified": "2025-08-20T14:32:39Z" } }, { "key": "AYIGVL9R", "version": 104, "library": { "type": "group", "id": 159246, "name": "NMR group", "links": { "alternate": { "href": "https://www.zotero.org/groups/nmr_group", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/159246/items/AYIGVL9R", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/nmr_group/items/AYIGVL9R", "type": "text/html" } }, "meta": { "createdByUser": { "id": 283300, "username": "glewin", "name": "Gary Lewin", "links": { "alternate": { "href": "https://www.zotero.org/glewin", "type": "text/html" } } }, "creatorSummary": "Shin et al.", "parsedDate": "2024-03", "numChildren": 1 }, "data": { "key": "AYIGVL9R", "version": 104, "itemType": "journalArticle", "title": "Identification and comparison of aroma and taste-related compounds from breast meat of three breeds of Korean native chickens", "creators": [ { "creatorType": "author", "firstName": "Dong-Jin", "lastName": "Shin" }, { "creatorType": "author", "firstName": "Yousung", "lastName": "Jung" }, { "creatorType": "author", "firstName": "Dongwook", "lastName": "Kim" }, { "creatorType": "author", "firstName": "Cheorun", "lastName": "Jo" }, { "creatorType": "author", "firstName": "Ki-Chang", "lastName": "Nam" }, { "creatorType": "author", "firstName": "Jun-Heon", "lastName": "Lee" }, { "creatorType": "author", "firstName": "Hyo-Joon", "lastName": "Choo" }, { "creatorType": "author", "firstName": "Aera", "lastName": "Jang" } ], "abstractNote": "This study was aimed to identify and compare the taste-related compounds (nucleotide-related compounds, free amino acid contents, and fatty acid composition) and aroma (volatile organic compounds [VOC]) compounds in the chicken breast meat from 3 kinds of Korean native chicken (KNC), namely Hanhyup 3 (HH3), Woorimatdag 1 (WRMD1) and Woorimatdag 2 (WRMD2). Among the 3 breeds, WRMD1 had significantly higher IMP and AMP contents than HH3. WRMD2 exhibited higher levels of umami and sweet-taste amino acids and oleic acid composition compared to HH3 (P < 0.05). HH3 showed a higher composition of unsaturated fatty acids than WRMD2 (P < 0.05). On their discrimination by flavor composition, some compounds including aspartic acid were analyzed as important compounds. Regarding aroma compounds, unique aroma compounds were detected for each breed and some compounds such as isopropyl myristate, p-cresol, (S)-(+)-3-Methyl-1-pentanol, and cyclic octa-atomic sulfur were expected to be utilized as key compounds in discrimination of the 3 breeds. From the result of this study, the differences on the flavor compounds of three breeds were elucidated and key compounds for their discrimination were presented.", "publicationTitle": "Poultry Science", "publisher": "", "place": "", "date": "2024-03", "volume": "103", "issue": "3", "section": "", "partNumber": "", "partTitle": "", "pages": "103462", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "Poult Sci", "DOI": "10.1016/j.psj.2024.103462", "citationKey": "", "url": "", "accessDate": "", "PMID": "", "PMCID": "", "ISSN": "1525-3171", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "eng", "libraryCatalog": "PubMed", "callNumber": "", "rights": "", "extra": "PMID: 38281330\nPMCID: PMC10840104", "tags": [ { "tag": "Amino Acids", "type": 1 }, { "tag": "Animals", "type": 1 }, { "tag": "Chickens", "type": 1 }, { "tag": "Korean native chicken", "type": 1 }, { "tag": "Meat", "type": 1 }, { "tag": "Odorants", "type": 1 }, { "tag": "Republic of Korea", "type": 1 }, { "tag": "Taste", "type": 1 }, { "tag": "Woorimatdag", "type": 1 }, { "tag": "flavor compound", "type": 1 }, { "tag": "taste-related compound", "type": 1 }, { "tag": "volatile organic compound", "type": 1 } ], "collections": [], "relations": {}, "dateAdded": "2025-08-20T14:32:38Z", "dateModified": "2025-08-20T14:32:38Z" } }, { "key": "7RGCASQ9", "version": 103, "library": { "type": "group", "id": 159246, "name": "NMR group", "links": { "alternate": { "href": "https://www.zotero.org/groups/nmr_group", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/159246/items/7RGCASQ9", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/nmr_group/items/7RGCASQ9", "type": "text/html" }, "up": { "href": "https://api.zotero.org/groups/159246/items/L4WKRUQV", "type": "application/json" } }, "meta": { "createdByUser": { "id": 283300, "username": "glewin", "name": "Gary Lewin", "links": { "alternate": { "href": "https://www.zotero.org/glewin", "type": "text/html" } } } }, "data": { "key": "7RGCASQ9", "version": 103, "parentItem": "L4WKRUQV", "itemType": "attachment", "linkMode": "linked_url", "title": "PubMed entry", "accessDate": "2025-08-20T14:02:33Z", "url": "http://www.ncbi.nlm.nih.gov/pubmed/36283785", "note": "", "contentType": "text/html", "charset": "", "tags": [], "relations": {}, "dateAdded": "2025-08-20T14:02:33Z", "dateModified": "2025-08-20T14:02:33Z" } }, { "key": "L4WKRUQV", "version": 103, "library": { "type": "group", "id": 159246, "name": "NMR group", "links": { "alternate": { "href": "https://www.zotero.org/groups/nmr_group", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/159246/items/L4WKRUQV", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/nmr_group/items/L4WKRUQV", "type": "text/html" } }, "meta": { "createdByUser": { "id": 283300, "username": "glewin", "name": "Gary Lewin", "links": { "alternate": { "href": "https://www.zotero.org/glewin", "type": "text/html" } } }, "creatorSummary": "Zhang et al.", "parsedDate": "2022-11-15", "numChildren": 1 }, "data": { "key": "L4WKRUQV", "version": 103, "itemType": "journalArticle", "title": "Identifying robust and reliable volatile organic compounds in human sebum for biomarker discovery", "creators": [ { "creatorType": "author", "firstName": "J. Diana", "lastName": "Zhang" }, { "creatorType": "author", "firstName": "Minh N.", "lastName": "Le" }, { "creatorType": "author", "firstName": "Kathryn J.", "lastName": "Hill" }, { "creatorType": "author", "firstName": "Antony A.", "lastName": "Cooper" }, { "creatorType": "author", "firstName": "Richard M.", "lastName": "Stuetz" }, { "creatorType": "author", "firstName": "William A.", "lastName": "Donald" } ], "abstractNote": "Sebum from sebaceous glands is a rich source of volatile organic compounds (VOCs) that can readily be sampled non-invasively from the surface of skin. The VOC profiles of sebum can then be used to obtain information regarding different medical conditions including diabetes and Parkinson's Disease. However, the effects of sampling approaches and environmental factors on sebum VOC profiles are not established and the confident attribution of VOCs to disease states needs to be free of extraneous influences such as sampling materials and preparatory conditions. Here, we investigated a more standardised skin swab sampling approach for profiling sebum VOCs from healthy human subjects using thermal desorption-gas chromatography-mass spectrometry (TD-GC-MS). Using a standard GC-MS method for the chemical analysis of sebum swabs, a surprisingly high number of VOCs originate from 'blank' medical swab material alone (up to 74 VOCs) and from the ambient environment (up to 29 VOCs) based on control experiments. We found that heat-treatment of medical swabs prior to GC-MS reduced the number of VOCs detected from 'blank' swabs and improved the reproducibility of VOC profiling, however significant VOC absorption can still occur from environmental exposure to ambient air. VOCs identified in 'blank' swabs consisted predominantly of hydrocarbons, esters, and silicon-based compounds and depended strongly on the material used (cotton and polyester-rayon). Environmental VOCs found to absorb to swabs from the ambient air during sampling included 1-butylheptyl-benzene and hexadecanoic acid methyl ester as well as exogenous VOCs such as isopropyl palmitate and isopropyl myristate. In contrast, sebum VOCs consisted primarily of esters, alcohols, ketones, and aldehydes. 23 and 18 VOCs were identified in sebum collected using polyester-rayon and cotton-based medical swabs, respectively, with 14 VOCs common to both swabs. The effect of subject bathing prior to sebum sampling had minimal impact on the VOC profiles. However, individual differences owing to external factors such as skin type, diet, and exercise will likely influence sebum production. This study highlights the importance of using rigorous controls in sebum sampling, and recommendations are provided for future research involving sebum VOC analysis. For example, the use of sebum sample replicates across multiple days, and the use of control swabs during sample collection is required to confirm the origin and reliability of sebum VOCs. It is anticipated that these recommendations in conjunction with a library of well-established VOCs from medical swabs will further strengthen biomarker identification resulting from sebum VOC analysis.", "publicationTitle": "Analytica Chimica Acta", "publisher": "", "place": "", "date": "2022-11-15", "volume": "1233", "issue": "", "section": "", "partNumber": "", "partTitle": "", "pages": "340506", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "Anal Chim Acta", "DOI": "10.1016/j.aca.2022.340506", "citationKey": "", "url": "", "accessDate": "", "PMID": "", "PMCID": "", "ISSN": "1873-4324", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "eng", "libraryCatalog": "PubMed", "callNumber": "", "rights": "", "extra": "PMID: 36283785", "tags": [ { "tag": "Air Pollutants", "type": 1 }, { "tag": "Aldehydes", "type": 1 }, { "tag": "Benzene", "type": 1 }, { "tag": "Biomarkers", "type": 1 }, { "tag": "Environmental Monitoring", "type": 1 }, { "tag": "Esters", "type": 1 }, { "tag": "Gas Chromatography-Mass Spectrometry", "type": 1 }, { "tag": "Humans", "type": 1 }, { "tag": "Hydrocarbons", "type": 1 }, { "tag": "Ketones", "type": 1 }, { "tag": "Palmitic Acid", "type": 1 }, { "tag": "Polyesters", "type": 1 }, { "tag": "Reproducibility of Results", "type": 1 }, { "tag": "Sebum", "type": 1 }, { "tag": "Silicon", "type": 1 }, { "tag": "Volatile Organic Compounds", "type": 1 } ], "collections": [], "relations": {}, "dateAdded": "2025-08-20T14:02:32Z", "dateModified": "2025-08-20T14:02:32Z" } }, { "key": "QS7NKTNC", "version": 102, "library": { "type": "group", "id": 159246, "name": "NMR group", "links": { "alternate": { "href": "https://www.zotero.org/groups/nmr_group", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/159246/items/QS7NKTNC", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/nmr_group/items/QS7NKTNC", "type": "text/html" } }, "meta": { "createdByUser": { "id": 283300, "username": "glewin", "name": "Gary Lewin", "links": { "alternate": { "href": "https://www.zotero.org/glewin", "type": "text/html" } } }, "creatorSummary": "Vladimirov et al.", "parsedDate": "2024-03-27", "numChildren": 0 }, "data": { "key": "QS7NKTNC", "version": 102, "itemType": "journalArticle", "title": "Benchtop mesoSPIM: a next-generation open-source light-sheet microscope for cleared samples", "creators": [ { "creatorType": "author", "firstName": "Nikita", "lastName": "Vladimirov" }, { "creatorType": "author", "firstName": "Fabian F.", "lastName": "Voigt" }, { "creatorType": "author", "firstName": "Thomas", "lastName": "Naert" }, { "creatorType": "author", "firstName": "Gabriela R.", "lastName": "Araujo" }, { "creatorType": "author", "firstName": "Ruiyao", "lastName": "Cai" }, { "creatorType": "author", "firstName": "Anna Maria", "lastName": "Reuss" }, { "creatorType": "author", "firstName": "Shan", "lastName": "Zhao" }, { "creatorType": "author", "firstName": "Patricia", "lastName": "Schmid" }, { "creatorType": "author", "firstName": "Sven", "lastName": "Hildebrand" }, { "creatorType": "author", "firstName": "Martina", "lastName": "Schaettin" }, { "creatorType": "author", "firstName": "Dominik", "lastName": "Groos" }, { "creatorType": "author", "firstName": "José María", "lastName": "Mateos" }, { "creatorType": "author", "firstName": "Philipp", "lastName": "Bethge" }, { "creatorType": "author", "firstName": "Taiyo", "lastName": "Yamamoto" }, { "creatorType": "author", "firstName": "Valentino", "lastName": "Aerne" }, { "creatorType": "author", "firstName": "Alard", "lastName": "Roebroeck" }, { "creatorType": "author", "firstName": "Ali", "lastName": "Ertürk" }, { "creatorType": "author", "firstName": "Adriano", "lastName": "Aguzzi" }, { "creatorType": "author", "firstName": "Urs", "lastName": "Ziegler" }, { "creatorType": "author", "firstName": "Esther", "lastName": "Stoeckli" }, { "creatorType": "author", "firstName": "Laura", "lastName": "Baudis" }, { "creatorType": "author", "firstName": "Soeren S.", "lastName": "Lienkamp" }, { "creatorType": "author", "firstName": "Fritjof", "lastName": "Helmchen" } ], "abstractNote": "In 2015, we launched the mesoSPIM initiative, an open-source project for making light-sheet microscopy of large cleared tissues more accessible. Meanwhile, the demand for imaging larger samples at higher speed and resolution has increased, requiring major improvements in the capabilities of such microscopes. Here, we introduce the next-generation mesoSPIM (“Benchtop”) with a significantly increased field of view, improved resolution, higher throughput, more affordable cost, and simpler assembly compared to the original version. We develop an optical method for testing detection objectives that enables us to select objectives optimal for light-sheet imaging with large-sensor cameras. The improved mesoSPIM achieves high spatial resolution (1.5 µm laterally, 3.3 µm axially) across the entire field of view, magnification up to 20×, and supports sample sizes ranging from sub-mm up to several centimeters while being compatible with multiple clearing techniques. The microscope serves a broad range of applications in neuroscience, developmental biology, pathology, and even physics.", "publicationTitle": "Nature Communications", "publisher": "", "place": "", "date": "2024-03-27", "volume": "15", "issue": "1", "section": "", "partNumber": "", "partTitle": "", "pages": "2679", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "Nat Commun", "DOI": "10.1038/s41467-024-46770-2", "citationKey": "", "url": "https://www.nature.com/articles/s41467-024-46770-2", "accessDate": "2025-08-20T12:10:25Z", "PMID": "", "PMCID": "", "ISSN": "2041-1723", "archive": "", "archiveLocation": "", "shortTitle": "Benchtop mesoSPIM", "language": "en", "libraryCatalog": "www.nature.com", "callNumber": "", "rights": "2024 The Author(s)", "extra": "Publisher: Nature Publishing Group", "tags": [ { "tag": "Fluorescence imaging", "type": 1 }, { "tag": "Light-sheet microscopy", "type": 1 }, { "tag": "Particle astrophysics", "type": 1 } ], "collections": [], "relations": {}, "dateAdded": "2025-08-20T12:10:25Z", "dateModified": "2025-08-20T12:10:25Z" } }, { "key": "G286ELDJ", "version": 101, "library": { "type": "group", "id": 159246, "name": "NMR group", "links": { "alternate": { "href": "https://www.zotero.org/groups/nmr_group", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/159246/items/G286ELDJ", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/nmr_group/items/G286ELDJ", "type": "text/html" } }, "meta": { "createdByUser": { "id": 283300, "username": "glewin", "name": "Gary Lewin", "links": { "alternate": { "href": "https://www.zotero.org/glewin", "type": "text/html" } } }, "creatorSummary": "Camoletto et al.", "parsedDate": "2001-01-01", "numChildren": 1 }, "data": { "key": "G286ELDJ", "version": 101, "itemType": "journalArticle", "title": "Expression of stathmin and SCG10 proteins in the olfactory neurogenesis during development and after lesion in the adulthood", "creators": [ { "creatorType": "author", "firstName": "P.", "lastName": "Camoletto" }, { "creatorType": "author", "firstName": "A.", "lastName": "Colesanti" }, { "creatorType": "author", "firstName": "S.", "lastName": "Ozon" }, { "creatorType": "author", "firstName": "A.", "lastName": "Sobel" }, { "creatorType": "author", "firstName": "A.", "lastName": "Fasolo" } ], "abstractNote": "Stathmin and SCG10 belong to a family of phosphoproteins associated to cell proliferation and differentiation. In the present study, we have analyzed immunocytochemically the distribution of these proteins during neurogenesis in the mouse olfactory system, from midgestation to adulthood. Data show that already at embryonic day 12, stathmin and SCG10 immunoreactivities were present in the olfactory and vomeronasal neurons, and their number increased greatly, colocalizing with neuronal specific tubulin, a marker of immature neurons. Later on up to adulthood, the distribution of stathmin and SCG10 became progressively restricted to a few immature receptor and chemosensory neurons. Significantly, in the olfactory epithelium, stathmin was seen in immature neurons and also in basal cells representing precursors of neuronal elements. Interestingly, before birth stathmin and SCG10 immunopositive cells were seen outside the olfactory epithelium, seemingly migrating toward the olfactory bulb. After regeneration in the adult following peripheral lesion of the olfactory epithelium, stathmin and SCG10 were again strongly expressed and generally colocalized with neuronal specific tubulin immunoreactivity. Overall these results indicate that stathmin and SCG10 are expressed in immature olfactory neurons as well as in the migrating cells generated from the olfactory epithelium, supporting the role of these proteins in neurogenesis and cell migration.", "publicationTitle": "Brain Research Bulletin", "publisher": "", "place": "", "date": "2001-01-01", "volume": "54", "issue": "1", "section": "", "partNumber": "", "partTitle": "", "pages": "19-28", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "Brain Res Bull", "DOI": "10.1016/s0361-9230(00)00412-3", "citationKey": "", "url": "", "accessDate": "", "PMID": "", "PMCID": "", "ISSN": "0361-9230", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "eng", "libraryCatalog": "PubMed", "callNumber": "", "rights": "", "extra": "PMID: 11226711", "tags": [ { "tag": "Animals", "type": 1 }, { "tag": "Calcium-Binding Proteins", "type": 1 }, { "tag": "Denervation", "type": 1 }, { "tag": "Female", "type": 1 }, { "tag": "Fetus", "type": 1 }, { "tag": "Immunohistochemistry", "type": 1 }, { "tag": "Intracellular Signaling Peptides and Proteins", "type": 1 }, { "tag": "Mice", "type": 1 }, { "tag": "Mice, Inbred Strains", "type": 1 }, { "tag": "Microtubule Proteins", "type": 1 }, { "tag": "Nerve Growth Factors", "type": 1 }, { "tag": "Nerve Regeneration", "type": 1 }, { "tag": "Nerve Tissue Proteins", "type": 1 }, { "tag": "Olfactory Marker Protein", "type": 1 }, { "tag": "Olfactory Mucosa", "type": 1 }, { "tag": "Olfactory Receptor Neurons", "type": 1 }, { "tag": "Phosphoproteins", "type": 1 }, { "tag": "Pregnancy", "type": 1 }, { "tag": "Stathmin", "type": 1 }, { "tag": "Tubulin", "type": 1 } ], "collections": [], "relations": {}, "dateAdded": "2025-08-20T09:01:12Z", "dateModified": "2025-08-20T09:01:12Z" } }, { "key": "M5FPQS4S", "version": 101, "library": { "type": "group", "id": 159246, "name": "NMR group", "links": { "alternate": { "href": "https://www.zotero.org/groups/nmr_group", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/159246/items/M5FPQS4S", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/nmr_group/items/M5FPQS4S", "type": "text/html" }, "up": { "href": "https://api.zotero.org/groups/159246/items/G286ELDJ", "type": "application/json" } }, "meta": { "createdByUser": { "id": 283300, "username": "glewin", "name": "Gary Lewin", "links": { "alternate": { "href": "https://www.zotero.org/glewin", "type": "text/html" } } } }, "data": { "key": "M5FPQS4S", "version": 101, "parentItem": "G286ELDJ", "itemType": "attachment", "linkMode": "linked_url", "title": "PubMed entry", "accessDate": "2025-08-20T09:01:12Z", "url": "http://www.ncbi.nlm.nih.gov/pubmed/11226711", "note": "", "contentType": "text/html", "charset": "", "tags": [], "relations": {}, "dateAdded": "2025-08-20T09:01:12Z", "dateModified": "2025-08-20T09:01:12Z" } }, { "key": "9EV2QFHM", "version": 100, "library": { "type": "group", "id": 159246, "name": "NMR group", "links": { "alternate": { "href": "https://www.zotero.org/groups/nmr_group", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/159246/items/9EV2QFHM", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/nmr_group/items/9EV2QFHM", "type": "text/html" } }, "meta": { "createdByUser": { "id": 283300, "username": "glewin", "name": "Gary Lewin", "links": { "alternate": { "href": "https://www.zotero.org/glewin", "type": "text/html" } } }, "creatorSummary": "Margolis", "parsedDate": "1972-05", "numChildren": 0 }, "data": { "key": "9EV2QFHM", "version": 100, "itemType": "journalArticle", "title": "A Brain Protein Unique to the Olfactory Bulb", "creators": [ { "creatorType": "author", "firstName": "Frank L.", "lastName": "Margolis" } ], "abstractNote": "Soluble extracts from several regions of the mouse brain manifested regionally specific protein band patterns on polyacrylamide gel electrophoresis. In particular, one protein band appeared to occur uniquely in the olfactory bulb extracts where it was a quantitatively significant constituent of the soluble protein extract. This protein was purified to homogeneity by ammonium sulfate precipitation, DEAE-cellulose column chromatography, and polyacrylamide gel electrophoresis. The purified protein has a minimum molecular weight of about 20,000 by gel electrophoresis in the presence of sodium dodecyl sulfate. Precipitating antiserum prepared against this protein reacted only with the purified protein or with extracts of olfactory bulb, and not with extracts of other mouse tissues or brain regions. Quantitative immunoprecipitation assays indicate that this specific protein represents 1% of the total soluble protein in the mouse olfactory bulb. Soluble extracts of olfactory bulbs from several rodent species gave reactions of identity by agar gel diffusion with the antiserum to the mouse protein. It is suggested that this protein is an example of selective genetic expression within the central nervous system.", "publicationTitle": "Proceedings of the National Academy of Sciences", "publisher": "", "place": "", "date": "1972-05", "volume": "69", "issue": "5", "section": "", "partNumber": "", "partTitle": "", "pages": "1221-1224", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "", "DOI": "10.1073/pnas.69.5.1221", "citationKey": "", "url": "https://www.pnas.org/doi/abs/10.1073/pnas.69.5.1221", "accessDate": "2025-08-20T08:51:02Z", "PMID": "", "PMCID": "", "ISSN": "", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "", "libraryCatalog": "pnas.org (Atypon)", "callNumber": "", "rights": "", "extra": "Publisher: Proceedings of the National Academy of Sciences", "tags": [], "collections": [], "relations": {}, "dateAdded": "2025-08-20T08:51:02Z", "dateModified": "2025-08-20T08:51:02Z" } }, { "key": "7XQXQBB7", "version": 99, "library": { "type": "group", "id": 159246, "name": "NMR group", "links": { "alternate": { "href": "https://www.zotero.org/groups/nmr_group", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/159246/items/7XQXQBB7", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/nmr_group/items/7XQXQBB7", "type": "text/html" }, "up": { "href": "https://api.zotero.org/groups/159246/items/6BXRG4M8", "type": "application/json" } }, "meta": { "createdByUser": { "id": 283300, "username": "glewin", "name": "Gary Lewin", "links": { "alternate": { "href": "https://www.zotero.org/glewin", "type": "text/html" } } } }, "data": { "key": "7XQXQBB7", "version": 99, "parentItem": "6BXRG4M8", "itemType": "attachment", "linkMode": "linked_url", "title": "PubMed entry", "accessDate": "2025-08-20T08:50:27Z", "url": "http://www.ncbi.nlm.nih.gov/pubmed/1100198", "note": "", "contentType": "text/html", "charset": "", "tags": [], "relations": {}, "dateAdded": "2025-08-20T08:50:27Z", "dateModified": "2025-08-20T08:50:27Z" } }, { "key": "6BXRG4M8", "version": 99, "library": { "type": "group", "id": 159246, "name": "NMR group", "links": { "alternate": { "href": "https://www.zotero.org/groups/nmr_group", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/159246/items/6BXRG4M8", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/nmr_group/items/6BXRG4M8", "type": "text/html" } }, "meta": { "createdByUser": { "id": 283300, "username": "glewin", "name": "Gary Lewin", "links": { "alternate": { "href": "https://www.zotero.org/glewin", "type": "text/html" } } }, "creatorSummary": "Hartman and Margolis", "parsedDate": "1975-10-10", "numChildren": 1 }, "data": { "key": "6BXRG4M8", "version": 99, "itemType": "journalArticle", "title": "Immunofluorescence localization of the olfactory marker protein", "creators": [ { "creatorType": "author", "firstName": "B. K.", "lastName": "Hartman" }, { "creatorType": "author", "firstName": "F. L.", "lastName": "Margolis" } ], "abstractNote": "", "publicationTitle": "Brain Research", "publisher": "", "place": "", "date": "1975-10-10", "volume": "96", "issue": "1", "section": "", "partNumber": "", "partTitle": "", "pages": "176-180", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "Brain Res", "DOI": "10.1016/0006-8993(75)90593-4", "citationKey": "", "url": "", "accessDate": "", "PMID": "", "PMCID": "", "ISSN": "0006-8993", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "eng", "libraryCatalog": "PubMed", "callNumber": "", "rights": "", "extra": "PMID: 1100198", "tags": [ { "tag": "Animals", "type": 1 }, { "tag": "Chemoreceptor Cells", "type": 1 }, { "tag": "Fluorescent Antibody Technique", "type": 1 }, { "tag": "Nerve Tissue Proteins", "type": 1 }, { "tag": "Olfactory Bulb", "type": 1 }, { "tag": "Olfactory Mucosa", "type": 1 }, { "tag": "Rats", "type": 1 } ], "collections": [], "relations": {}, "dateAdded": "2025-08-20T08:50:26Z", "dateModified": "2025-08-20T08:50:26Z" } }, { "key": "FASRZK25", "version": 98, "library": { "type": "group", "id": 159246, "name": "NMR group", "links": { "alternate": { "href": "https://www.zotero.org/groups/nmr_group", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/159246/items/FASRZK25", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/nmr_group/items/FASRZK25", "type": "text/html" } }, "meta": { "createdByUser": { "id": 283300, "username": "glewin", "name": "Gary Lewin", "links": { "alternate": { "href": "https://www.zotero.org/glewin", "type": "text/html" } } }, "creatorSummary": "Slessor et al.", "parsedDate": "2005-11-01", "numChildren": 0 }, "data": { "key": "FASRZK25", "version": 98, "itemType": "journalArticle", "title": "Pheromone Communication in the Honeybee (Apis mellifera L.)", "creators": [ { "creatorType": "author", "firstName": "Keith N.", "lastName": "Slessor" }, { "creatorType": "author", "firstName": "Mark L.", "lastName": "Winston" }, { "creatorType": "author", "firstName": "Yves", "lastName": "Le Conte" } ], "abstractNote": "Recent studies have demonstrated a remarkable and unexpected complexity in social insect pheromone communication, particularly for honeybees (Apis mellifera L.). The intricate interactions characteristic of social insects demand a complex language, based on specialized chemical signals that provide a syntax that is deeper in complexity and richer in nuance than previously imagined. Here, we discuss this rapidly evolving field for honeybees, the only social insect for which any primer pheromones have been identified. Novel research has demonstrated the importance of complexity, synergy, context, and dose, mediated through spatial and temporal pheromone distribution, and has revealed an unprecedented wealth of identified semiochemicals and functions. These new results demand fresh terminology, and we propose adding “colony pheromone” and “passenger pheromone” to the current terms sociochemical, releaser, and primer pheromone to better encompass our growing understanding of chemical communication in social insects.", "publicationTitle": "Journal of Chemical Ecology", "publisher": "", "place": "", "date": "2005-11-01", "volume": "31", "issue": "11", "section": "", "partNumber": "", "partTitle": "", "pages": "2731-2745", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "J Chem Ecol", "DOI": "10.1007/s10886-005-7623-9", "citationKey": "", "url": "https://doi.org/10.1007/s10886-005-7623-9", "accessDate": "2025-08-17T19:40:05Z", "PMID": "", "PMCID": "", "ISSN": "1573-1561", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "en", "libraryCatalog": "Springer Link", "callNumber": "", "rights": "", "extra": "", "tags": [ { "tag": "Apis mellifera", "type": 1 }, { "tag": "chemical communication", "type": 1 }, { "tag": "chemoecology", "type": 1 }, { "tag": "honeybee", "type": 1 }, { "tag": "pheromone", "type": 1 }, { "tag": "social insect", "type": 1 } ], "collections": [], "relations": {}, "dateAdded": "2025-08-17T19:40:05Z", "dateModified": "2025-08-17T19:40:05Z" } }, { "key": "PAZTDQA8", "version": 97, "library": { "type": "group", "id": 159246, "name": "NMR group", "links": { "alternate": { "href": "https://www.zotero.org/groups/nmr_group", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/159246/items/PAZTDQA8", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/nmr_group/items/PAZTDQA8", "type": "text/html" }, "up": { "href": "https://api.zotero.org/groups/159246/items/GTRA3NB6", "type": "application/json" } }, "meta": { "createdByUser": { "id": 283300, "username": "glewin", "name": "Gary Lewin", "links": { "alternate": { "href": "https://www.zotero.org/glewin", "type": "text/html" } } } }, "data": { "key": "PAZTDQA8", "version": 97, "parentItem": "GTRA3NB6", "itemType": "attachment", "linkMode": "linked_url", "title": "PubMed entry", "accessDate": "2025-08-17T19:28:28Z", "url": "http://www.ncbi.nlm.nih.gov/pubmed/17877458", "note": "", "contentType": "text/html", "charset": "", "tags": [], "relations": {}, "dateAdded": "2025-08-17T19:28:28Z", "dateModified": "2025-08-17T19:28:28Z" } }, { "key": "GTRA3NB6", "version": 97, "library": { "type": "group", "id": 159246, "name": "NMR group", "links": { "alternate": { "href": "https://www.zotero.org/groups/nmr_group", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/159246/items/GTRA3NB6", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/nmr_group/items/GTRA3NB6", "type": "text/html" } }, "meta": { "createdByUser": { "id": 283300, "username": "glewin", "name": "Gary Lewin", "links": { "alternate": { "href": "https://www.zotero.org/glewin", "type": "text/html" } } }, "creatorSummary": "Le Conte and Hefetz", "parsedDate": "2008", "numChildren": 1 }, "data": { "key": "GTRA3NB6", "version": 97, "itemType": "journalArticle", "title": "Primer pheromones in social hymenoptera", "creators": [ { "creatorType": "author", "firstName": "Yves", "lastName": "Le Conte" }, { "creatorType": "author", "firstName": "Abraham", "lastName": "Hefetz" } ], "abstractNote": "Social insect are profoundly influenced by primer pheromones (PPhs), which are efficient means for maintaining social harmony in the colony. PPhs act by affecting the physiology of the recipients with a subsequent shift in their behavior, and many PPhs have a releaser effect (i.e., changing the probability of performing a certain behavior upon perception). In this review we try to clarify the interplay between such dual pheromonal effects. Only a few PPhs have been identified, and we provide evidence for their existence in multiple species of social Hymenoptera, which is the most extensively studied of the social insects. We focus on the regulation of reproduction, social policing, and task allocation. Considering PPhs in a broad sense, we also discuss fertility signals and the role of cuticular hydrocarbons as putative PPhs. Identification of the underlying chemistry of PPhs offers insights into insect physiology and the evolution of social behavior. PPhs of the honey bee are used to demonstrate the complexity of pheromonal communication in social insects.", "publicationTitle": "Annual Review of Entomology", "publisher": "", "place": "", "date": "2008", "volume": "53", "issue": "", "section": "", "partNumber": "", "partTitle": "", "pages": "523-542", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "Annu Rev Entomol", "DOI": "10.1146/annurev.ento.52.110405.091434", "citationKey": "", "url": "", "accessDate": "", "PMID": "", "PMCID": "", "ISSN": "0066-4170", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "eng", "libraryCatalog": "PubMed", "callNumber": "", "rights": "", "extra": "PMID: 17877458", "tags": [ { "tag": "Animal Communication", "type": 1 }, { "tag": "Animals", "type": 1 }, { "tag": "Female", "type": 1 }, { "tag": "Fertility", "type": 1 }, { "tag": "Hydrocarbons", "type": 1 }, { "tag": "Hymenoptera", "type": 1 }, { "tag": "Male", "type": 1 }, { "tag": "Pheromones", "type": 1 }, { "tag": "Sexual Behavior, Animal", "type": 1 }, { "tag": "Social Behavior", "type": 1 } ], "collections": [], "relations": {}, "dateAdded": "2025-08-17T19:28:28Z", "dateModified": "2025-08-17T19:28:28Z" } }, { "key": "P4JG5TUC", "version": 96, "library": { "type": "group", "id": 159246, "name": "NMR group", "links": { "alternate": { "href": "https://www.zotero.org/groups/nmr_group", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/159246/items/P4JG5TUC", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/nmr_group/items/P4JG5TUC", "type": "text/html" } }, "meta": { "createdByUser": { "id": 283300, "username": "glewin", "name": "Gary Lewin", "links": { "alternate": { "href": "https://www.zotero.org/glewin", "type": "text/html" } } }, "creatorSummary": "Van Oystaeyen et al.", "parsedDate": "2014-01-17", "numChildren": 0 }, "data": { "key": "P4JG5TUC", "version": 96, "itemType": "journalArticle", "title": "Conserved Class of Queen Pheromones Stops Social Insect Workers from Reproducing", "creators": [ { "creatorType": "author", "firstName": "Annette", "lastName": "Van Oystaeyen" }, { "creatorType": "author", "firstName": "Ricardo Caliari", "lastName": "Oliveira" }, { "creatorType": "author", "firstName": "Luke", "lastName": "Holman" }, { "creatorType": "author", "firstName": "Jelle S.", "lastName": "van Zweden" }, { "creatorType": "author", "firstName": "Carmen", "lastName": "Romero" }, { "creatorType": "author", "firstName": "Cintia A.", "lastName": "Oi" }, { "creatorType": "author", "firstName": "Patrizia", "lastName": "d'Ettorre" }, { "creatorType": "author", "firstName": "Mohammadreza", "lastName": "Khalesi" }, { "creatorType": "author", "firstName": "Johan", "lastName": "Billen" }, { "creatorType": "author", "firstName": "Felix", "lastName": "Wäckers" }, { "creatorType": "author", "firstName": "Jocelyn G.", "lastName": "Millar" }, { "creatorType": "author", "firstName": "Tom", "lastName": "Wenseleers" } ], "abstractNote": "A major evolutionary transition to eusociality with reproductive division of labor between queens and workers has arisen independently at least 10 times in the ants, bees, and wasps. Pheromones produced by queens are thought to play a key role in regulating this complex social system, but their evolutionary history remains unknown. Here, we identify the first sterility-inducing queen pheromones in a wasp, bumblebee, and desert ant and synthesize existing data on compounds that characterize female fecundity in 64 species of social insects. Our results show that queen pheromones are strikingly conserved across at least three independent origins of eusociality, with wasps, ants, and some bees all appearing to use nonvolatile, saturated hydrocarbons to advertise fecundity and/or suppress worker reproduction. These results suggest that queen pheromones evolved from conserved signals of solitary ancestors.", "publicationTitle": "Science", "publisher": "", "place": "", "date": "2014-01-17", "volume": "343", "issue": "6168", "section": "", "partNumber": "", "partTitle": "", "pages": "287-290", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "", "DOI": "10.1126/science.1244899", "citationKey": "", "url": "https://www.science.org/doi/10.1126/science.1244899", "accessDate": "2025-08-17T19:22:08Z", "PMID": "", "PMCID": "", "ISSN": "", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "", "libraryCatalog": "science.org (Atypon)", "callNumber": "", "rights": "", "extra": "Publisher: American Association for the Advancement of Science", "tags": [], "collections": [], "relations": {}, "dateAdded": "2025-08-17T19:22:08Z", "dateModified": "2025-08-17T19:22:08Z" } }, { "key": "YL5LAVKP", "version": 95, "library": { "type": "group", "id": 159246, "name": "NMR group", "links": { "alternate": { "href": "https://www.zotero.org/groups/nmr_group", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/159246/items/YL5LAVKP", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/nmr_group/items/YL5LAVKP", "type": "text/html" } }, "meta": { "createdByUser": { "id": 283300, "username": "glewin", "name": "Gary Lewin", "links": { "alternate": { "href": "https://www.zotero.org/glewin", "type": "text/html" } } }, "creatorSummary": "Van Oystaeyen et al.", "parsedDate": "2014-01-17", "numChildren": 0 }, "data": { "key": "YL5LAVKP", "version": 95, "itemType": "journalArticle", "title": "Conserved Class of Queen Pheromones Stops Social Insect Workers from Reproducing", "creators": [ { "creatorType": "author", "firstName": "Annette", "lastName": "Van Oystaeyen" }, { "creatorType": "author", "firstName": "Ricardo Caliari", "lastName": "Oliveira" }, { "creatorType": "author", "firstName": "Luke", "lastName": "Holman" }, { "creatorType": "author", "firstName": "Jelle S.", "lastName": "van Zweden" }, { "creatorType": "author", "firstName": "Carmen", "lastName": "Romero" }, { "creatorType": "author", "firstName": "Cintia A.", "lastName": "Oi" }, { "creatorType": "author", "firstName": "Patrizia", "lastName": "d'Ettorre" }, { "creatorType": "author", "firstName": "Mohammadreza", "lastName": "Khalesi" }, { "creatorType": "author", "firstName": "Johan", "lastName": "Billen" }, { "creatorType": "author", "firstName": "Felix", "lastName": "Wäckers" }, { "creatorType": "author", "firstName": "Jocelyn G.", "lastName": "Millar" }, { "creatorType": "author", "firstName": "Tom", "lastName": "Wenseleers" } ], "abstractNote": "A major evolutionary transition to eusociality with reproductive division of labor between queens and workers has arisen independently at least 10 times in the ants, bees, and wasps. Pheromones produced by queens are thought to play a key role in regulating this complex social system, but their evolutionary history remains unknown. Here, we identify the first sterility-inducing queen pheromones in a wasp, bumblebee, and desert ant and synthesize existing data on compounds that characterize female fecundity in 64 species of social insects. Our results show that queen pheromones are strikingly conserved across at least three independent origins of eusociality, with wasps, ants, and some bees all appearing to use nonvolatile, saturated hydrocarbons to advertise fecundity and/or suppress worker reproduction. These results suggest that queen pheromones evolved from conserved signals of solitary ancestors.", "publicationTitle": "Science", "publisher": "", "place": "", "date": "2014-01-17", "volume": "343", "issue": "6168", "section": "", "partNumber": "", "partTitle": "", "pages": "287-290", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "", "DOI": "10.1126/science.1244899", "citationKey": "", "url": "https://www.science.org/doi/10.1126/science.1244899", "accessDate": "2025-08-17T19:20:46Z", "PMID": "", "PMCID": "", "ISSN": "", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "", "libraryCatalog": "science.org (Atypon)", "callNumber": "", "rights": "", "extra": "Publisher: American Association for the Advancement of Science", "tags": [], "collections": [], "relations": {}, "dateAdded": "2025-08-17T19:20:46Z", "dateModified": "2025-08-17T19:20:46Z" } }, { "key": "4K9ZGIQA", "version": 94, "library": { "type": "group", "id": 159246, "name": "NMR group", "links": { "alternate": { "href": "https://www.zotero.org/groups/nmr_group", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/159246/items/4K9ZGIQA", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/nmr_group/items/4K9ZGIQA", "type": "text/html" } }, "meta": { "createdByUser": { "id": 283300, "username": "glewin", "name": "Gary Lewin", "links": { "alternate": { "href": "https://www.zotero.org/glewin", "type": "text/html" } } }, "creatorSummary": "Murata et al.", "parsedDate": "2014-03-17", "numChildren": 0 }, "data": { "key": "4K9ZGIQA", "version": 94, "itemType": "journalArticle", "title": "Identification of an Olfactory Signal Molecule that Activates the Central Regulator of Reproduction in Goats", "creators": [ { "creatorType": "author", "firstName": "Ken", "lastName": "Murata" }, { "creatorType": "author", "firstName": "Shigeyuki", "lastName": "Tamogami" }, { "creatorType": "author", "firstName": "Masamichi", "lastName": "Itou" }, { "creatorType": "author", "firstName": "Yasutaka", "lastName": "Ohkubo" }, { "creatorType": "author", "firstName": "Yoshihiro", "lastName": "Wakabayashi" }, { "creatorType": "author", "firstName": "Hidenori", "lastName": "Watanabe" }, { "creatorType": "author", "firstName": "Hiroaki", "lastName": "Okamura" }, { "creatorType": "author", "firstName": "Yukari", "lastName": "Takeuchi" }, { "creatorType": "author", "firstName": "Yuji", "lastName": "Mori" } ], "abstractNote": "Pheromone signals regulate conspecific behavior and physiology [1]. Releaser pheromones induce specific behavior by exerting acute effects on the neuronal response, whereas primer pheromones induce physiological changes with long-lasting effects by changing the neuroendocrine status of the recipients. In mammals, although several types of releaser pheromones have been identified [2, 3, 4, 5], the identities of primer pheromones, as well as their mechanisms of action, remain largely unknown [6]. In sheep and goats, the seasonally anestrous endocrine state of females is changed to the estrous state upon exposure to male scents [7, 8]. This so-called “male effect” is one of the most conspicuous primer pheromone effects in mammals [9, 10]. In this study, we have identified an olfactory signal molecule that activates the central regulator of reproduction, the gonadotropin-releasing hormone (GnRH) pulse generator, in goats. Using gas chromatography-mass spectrometry to analyze male goat headspace volatiles, we identified several ethyl-branched aldehydes and ketones. We electrophysiologically demonstrated that one of these compounds, 4-ethyloctanal, activates the GnRH pulse generator in female goats. This is the first report of an olfactory molecule that has been shown to activate the central reproductive axis, and this discovery will provide a new direction for primer pheromone research.", "publicationTitle": "Current Biology", "publisher": "", "place": "", "date": "2014-03-17", "volume": "24", "issue": "6", "section": "", "partNumber": "", "partTitle": "", "pages": "681-686", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "Current Biology", "DOI": "10.1016/j.cub.2014.01.073", "citationKey": "", "url": "https://www.sciencedirect.com/science/article/pii/S0960982214001407", "accessDate": "2025-08-17T17:25:30Z", "PMID": "", "PMCID": "", "ISSN": "0960-9822", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "", "libraryCatalog": "ScienceDirect", "callNumber": "", "rights": "", "extra": "", "tags": [], "collections": [], "relations": {}, "dateAdded": "2025-08-17T17:25:30Z", "dateModified": "2025-08-17T17:25:30Z" } }, { "key": "UPJ4557T", "version": 93, "library": { "type": "group", "id": 159246, "name": "NMR group", "links": { "alternate": { "href": "https://www.zotero.org/groups/nmr_group", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/159246/items/UPJ4557T", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/nmr_group/items/UPJ4557T", "type": "text/html" }, "up": { "href": "https://api.zotero.org/groups/159246/items/NHZ38XNC", "type": "application/json" } }, "meta": { "createdByUser": { "id": 283300, "username": "glewin", "name": "Gary Lewin", "links": { "alternate": { "href": "https://www.zotero.org/glewin", "type": "text/html" } } } }, "data": { "key": "UPJ4557T", "version": 93, "parentItem": "NHZ38XNC", "itemType": "attachment", "linkMode": "linked_url", "title": "PubMed entry", "accessDate": "2025-08-17T11:54:11Z", "url": "http://www.ncbi.nlm.nih.gov/pubmed/38741464", "note": "", "contentType": "text/html", "charset": "", "tags": [], "relations": {}, "dateAdded": "2025-08-17T11:54:11Z", "dateModified": "2025-08-17T11:54:11Z" } }, { "key": "NHZ38XNC", "version": 93, "library": { "type": "group", "id": 159246, "name": "NMR group", "links": { "alternate": { "href": "https://www.zotero.org/groups/nmr_group", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/159246/items/NHZ38XNC", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/nmr_group/items/NHZ38XNC", "type": "text/html" } }, "meta": { "createdByUser": { "id": 283300, "username": "glewin", "name": "Gary Lewin", "links": { "alternate": { "href": "https://www.zotero.org/glewin", "type": "text/html" } } }, "creatorSummary": "Arcas et al.", "parsedDate": "2024-09", "numChildren": 1 }, "data": { "key": "NHZ38XNC", "version": 93, "itemType": "journalArticle", "title": "The ion channel TRPM8 is a direct target of the immunosuppressant rapamycin in primary sensory neurons", "creators": [ { "creatorType": "author", "firstName": "José Miguel", "lastName": "Arcas" }, { "creatorType": "author", "firstName": "Khalid", "lastName": "Oudaha" }, { "creatorType": "author", "firstName": "Alejandro", "lastName": "González" }, { "creatorType": "author", "firstName": "Jorge", "lastName": "Fernández-Trillo" }, { "creatorType": "author", "firstName": "Francisco Andrés", "lastName": "Peralta" }, { "creatorType": "author", "firstName": "Júlia", "lastName": "Castro-Marsal" }, { "creatorType": "author", "firstName": "Seyma", "lastName": "Poyraz" }, { "creatorType": "author", "firstName": "Francisco", "lastName": "Taberner" }, { "creatorType": "author", "firstName": "Salvador", "lastName": "Sala" }, { "creatorType": "author", "firstName": "Elvira", "lastName": "de la Peña" }, { "creatorType": "author", "firstName": "Ana", "lastName": "Gomis" }, { "creatorType": "author", "firstName": "Félix", "lastName": "Viana" } ], "abstractNote": "BACKGROUND AND PURPOSE: The mechanistic target of rapamycin (mTOR) signalling pathway is a key regulator of cell growth and metabolism. Its deregulation is implicated in several diseases. The macrolide rapamycin, a specific inhibitor of mTOR, has immunosuppressive, anti-inflammatory and antiproliferative properties. Recently, we identified tacrolimus, another macrolide immunosuppressant, as a novel activator of TRPM8 ion channels, involved in cold temperature sensing, thermoregulation, tearing and cold pain. We hypothesized that rapamycin may also have agonist activity on TRPM8 channels.\nEXPERIMENTAL APPROACH: Using calcium imaging and electrophysiology in transfected HEK293 cells and wildtype or Trpm8 KO mouse DRG neurons, we characterized rapamycin's effects on TRPM8 channels. We also examined the effects of rapamycin on tearing in mice.\nKEY RESULTS: Micromolar concentrations of rapamycin activated rat and mouse TRPM8 channels directly and potentiated cold-evoked responses, effects also observed in human TRPM8 channels. In cultured mouse DRG neurons, rapamycin increased intracellular calcium levels almost exclusively in cold-sensitive neurons. Responses were markedly decreased in Trpm8 KO mice or by TRPM8 channel antagonists. Cutaneous cold thermoreceptor endings were also activated by rapamycin. Topical application of rapamycin to the eye surface evokes tearing in mice by a TRPM8-dependent mechanism.\nCONCLUSION AND IMPLICATIONS: These results identify TRPM8 cationic channels in sensory neurons as novel molecular targets of the immunosuppressant rapamycin. These findings may help explain some of its therapeutic effects after topical application to the skin and the eye surface. Moreover, rapamycin could be used as an experimental tool in the clinic to explore cold thermoreceptors.", "publicationTitle": "British Journal of Pharmacology", "publisher": "", "place": "", "date": "2024-09", "volume": "181", "issue": "17", "section": "", "partNumber": "", "partTitle": "", "pages": "3192-3214", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "Br J Pharmacol", "DOI": "10.1111/bph.16402", "citationKey": "", "url": "", "accessDate": "", "PMID": "", "PMCID": "", "ISSN": "1476-5381", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "eng", "libraryCatalog": "PubMed", "callNumber": "", "rights": "", "extra": "PMID: 38741464", "tags": [ { "tag": "Animals", "type": 1 }, { "tag": "Calcium", "type": 1 }, { "tag": "Cells, Cultured", "type": 1 }, { "tag": "Cold Temperature", "type": 1 }, { "tag": "Ganglia, Spinal", "type": 1 }, { "tag": "HEK293 Cells", "type": 1 }, { "tag": "Humans", "type": 1 }, { "tag": "Immunosuppressive Agents", "type": 1 }, { "tag": "Male", "type": 1 }, { "tag": "Mice", "type": 1 }, { "tag": "Mice, Inbred C57BL", "type": 1 }, { "tag": "Mice, Knockout", "type": 1 }, { "tag": "Rats", "type": 1 }, { "tag": "Sensory Receptor Cells", "type": 1 }, { "tag": "Sirolimus", "type": 1 }, { "tag": "TRPM Cation Channels", "type": 1 }, { "tag": "ageing", "type": 1 }, { "tag": "cold", "type": 1 }, { "tag": "dry eye disease", "type": 1 }, { "tag": "mTOR", "type": 1 }, { "tag": "pain", "type": 1 }, { "tag": "thermoregulation", "type": 1 } ], "collections": [], "relations": {}, "dateAdded": "2025-08-17T11:54:10Z", "dateModified": "2025-08-17T11:54:10Z" } }, { "key": "FSR6RMW6", "version": 92, "library": { "type": "group", "id": 159246, "name": "NMR group", "links": { "alternate": { "href": "https://www.zotero.org/groups/nmr_group", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/159246/items/FSR6RMW6", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/nmr_group/items/FSR6RMW6", "type": "text/html" } }, "meta": { "createdByUser": { "id": 283300, "username": "glewin", "name": "Gary Lewin", "links": { "alternate": { "href": "https://www.zotero.org/glewin", "type": "text/html" } } }, "creatorSummary": "Reid et al.", "parsedDate": "2002-12-01", "numChildren": 1 }, "data": { "key": "FSR6RMW6", "version": 92, "itemType": "journalArticle", "title": "A cold- and menthol-activated current in rat dorsal root ganglion neurones: properties and role in cold transduction", "creators": [ { "creatorType": "author", "firstName": "Gordon", "lastName": "Reid" }, { "creatorType": "author", "firstName": "Alexandru", "lastName": "Babes" }, { "creatorType": "author", "firstName": "Florentina", "lastName": "Pluteanu" } ], "abstractNote": "Skin temperature is sensed by peripheral thermoreceptors. Using the neuronal soma in primary culture as a model of the receptor terminal, we have investigated the mechanisms of cold transduction in thermoreceptive neurones from rat dorsal root ganglia. Cold-sensitive neurones were pre-selected by screening for an increase in [Ca(2+)](i) on cooling; 49 % of them were also excited by 0.5 microM capsaicin. Action potentials and voltage-gated currents of cold-sensitive neurones were clearly distinct from those of cold-insensitive neurones. All cold-sensitive neurones expressed an inward current activated by cold and sensitised by (-)-menthol, which was absent from cold-insensitive neurones. This current was carried mainly by Na(+) ions and caused a depolarisation on cooling accompanied by action potentials, inducing voltage-gated Ca(2+) entry; a minor fraction of Ca(2+) entry was voltage-independent. Application of (-)-menthol shifted the threshold temperatures of the cold-induced depolarisation and the inward current to the same extent, indicating that the cold- and menthol-activated current normally sets the threshold temperature for depolarisation during cooling. The action of menthol was stereospecific, with the (+)-isomer being a less effective agonist than the (-)-isomer. Extracellular Ca(2+) modulated the cold- and menthol-activated current in a similar way to its action on intact cold receptors: lowered [Ca(2+)](o) sensitised the current, while raised [Ca(2+)](o) antagonised the menthol-induced sensitisation. During long cooling pulses the current showed adaptation, which depended on extracellular Ca(2+) and was mediated by a rise in [Ca(2+)](i). This adaptation consisted of a shift in the temperature sensitivity of the channel. In capsaicin-sensitive neurones, capsaicin application caused a profound depression of the cold-activated current. Inclusion of nerve growth factor in the culture medium shifted the threshold of the cold-activated current towards warmer temperatures. The current was blocked by 50 microM capsazepine and 100 microM SKF 96365. 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