JID: 7603509; aheadofprint
", "tags": [], "relations": {}, "dateAdded": "2013-06-28T20:27:40Z", "dateModified": "2013-07-17T19:57:20Z" } }, { "key": "HDH5R9JZ", "version": 40, "library": { "type": "group", "id": 151205, "name": "UW-Madison MSP", "links": { "alternate": { "href": "https://www.zotero.org/groups/uw-madison_msp", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/151205/items/HDH5R9JZ", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/uw-madison_msp/items/HDH5R9JZ", "type": "text/html" }, "up": { "href": "https://api.zotero.org/groups/151205/items/N6BH4T44", "type": "application/json" } }, "meta": { "createdByUser": { "id": 978886, "username": "schmidli", "name": "Laura Schmidli", "links": { "alternate": { "href": "https://www.zotero.org/schmidli", "type": "text/html" } } }, "numChildren": 0 }, "data": { "key": "HDH5R9JZ", "version": 40, "parentItem": "N6BH4T44", "itemType": "note", "note": "The following values have no corresponding Zotero field:PT: J; TC: 0; UT: WOS:000313929400003
", "tags": [], "relations": {}, "dateAdded": "2013-06-28T20:31:26Z", "dateModified": "2013-07-17T19:55:41Z" } }, { "key": "74ZX9SIP", "version": 40, "library": { "type": "group", "id": 151205, "name": "UW-Madison MSP", "links": { "alternate": { "href": "https://www.zotero.org/groups/uw-madison_msp", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/151205/items/74ZX9SIP", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/uw-madison_msp/items/74ZX9SIP", "type": "text/html" }, "up": { "href": "https://api.zotero.org/groups/151205/items/6ANQIJDT", "type": "application/json" } }, "meta": { "createdByUser": { "id": 978886, "username": "schmidli", "name": "Laura Schmidli", "links": { "alternate": { "href": "https://www.zotero.org/schmidli", "type": "text/html" } } } }, "data": { "key": "74ZX9SIP", "version": 40, "parentItem": "6ANQIJDT", "itemType": "attachment", "linkMode": "linked_url", "title": "PubMed entry", "accessDate": "2013-07-17T19:39:51Z", "url": "http://www.ncbi.nlm.nih.gov/pubmed/23010803", "note": "", "contentType": "text/html", "charset": "", "tags": [], "relations": {}, "dateAdded": "2013-07-17T19:39:51Z", "dateModified": "2013-07-17T19:55:21Z" } }, { "key": "6ANQIJDT", "version": 40, "library": { "type": "group", "id": 151205, "name": "UW-Madison MSP", "links": { "alternate": { "href": "https://www.zotero.org/groups/uw-madison_msp", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/151205/items/6ANQIJDT", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/uw-madison_msp/items/6ANQIJDT", "type": "text/html" } }, "meta": { "createdByUser": { "id": 978886, "username": "schmidli", "name": "Laura Schmidli", "links": { "alternate": { "href": "https://www.zotero.org/schmidli", "type": "text/html" } } }, "creatorSummary": "Sillay et al.", "parsedDate": "2013", "numChildren": 4 }, "data": { "key": "6ANQIJDT", "version": 40, "itemType": "journalArticle", "title": "Perioperative Brain Shift and Deep Brain Stimulating Electrode Deformation Analysis: Implications for rigid and non-rigid devices", "creators": [ { "creatorType": "author", "firstName": "Karl A.", "lastName": "Sillay" }, { "creatorType": "author", "firstName": "L. M.", "lastName": "Kumbier" }, { "creatorType": "author", "firstName": "C.", "lastName": "Ross" }, { "creatorType": "author", "firstName": "M.", "lastName": "Brady" }, { "creatorType": "author", "firstName": "A.", "lastName": "Alexander" }, { "creatorType": "author", "firstName": "A.", "lastName": "Gupta" }, { "creatorType": "author", "firstName": "N.", "lastName": "Adluru" }, { "creatorType": "author", "firstName": "G. S.", "lastName": "Miranpuri" }, { "creatorType": "author", "firstName": "J. C.", "lastName": "Williams" } ], "abstractNote": "Deep brain stimulation (DBS) efficacy is related to optimal electrode placement. Several authors have quantified brain shift related to surgical targeting; yet, few reports document and discuss the effects of brain shift after insertion. Objective: To quantify brain shift and electrode displacement after device insertion. Twelve patients were retrospectively reviewed, and one post-operative MRI and one time-delayed CT were obtained for each patient and their implanted electrodes modeled in 3D. Two competing methods were employed to measure the electrode tip location and deviation from the prototypical linear implant after the resolution of acute surgical changes, such as brain shift and pneumocephalus. In the interim between surgery and a pneumocephalus free postoperative scan, electrode deviation was documented in all patients and all electrodes. Significant shift of the electrode tip was identified in rostral, anterior, and medial directions (p < 0.05). Shift was greatest in the rostral direction, measuring an average of 1.41 mm. Brain shift and subsequent electrode displacement occurs in patients after DBS surgery with the reversal of intraoperative brain shift. Rostral displacement is on the order of the height of one DBS contact. Further investigation into the time course of intraoperative brain shift and its potential effects on procedures performed with rigid and non-rigid devices in supine and semi-sitting surgical positions is needed.", "publicationTitle": "Annals of Biomedical Engineering", "publisher": "", "place": "", "date": "2013", "volume": "41", "issue": "2", "section": "", "partNumber": "", "partTitle": "", "pages": "293-304", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "Ann.Biomed.Eng.", "DOI": "10.1007/s10439-012-0650-0", "citationKey": "", "url": "", "accessDate": "", "PMID": "", "PMCID": "", "ISSN": "0090-6964", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "", "libraryCatalog": "", "callNumber": "", "rights": "", "extra": "Journal Article", "tags": [ { "tag": "Aged", "type": 1 }, { "tag": "Brain", "type": 1 }, { "tag": "Deep Brain Stimulation", "type": 1 }, { "tag": "Electrodes, Implanted", "type": 1 }, { "tag": "Female", "type": 1 }, { "tag": "Humans", "type": 1 }, { "tag": "Magnetic Resonance Imaging", "type": 1 }, { "tag": "Male", "type": 1 }, { "tag": "Middle Aged", "type": 1 }, { "tag": "Monitoring, Intraoperative", "type": 1 }, { "tag": "Movement Disorders", "type": 1 }, { "tag": "Tomography, X-Ray Computed", "type": 1 } ], "collections": [ "DKSEB5FD" ], "relations": { "dc:replaces": [ "http://zotero.org/groups/151205/items/QEVTSI46", "http://zotero.org/groups/151205/items/T3THM8PM" ] }, "dateAdded": "2013-06-28T20:31:26Z", "dateModified": "2013-07-17T19:55:21Z" } }, { "key": "KE23B7G9", "version": 41, "library": { "type": "group", "id": 151205, "name": "UW-Madison MSP", "links": { "alternate": { "href": "https://www.zotero.org/groups/uw-madison_msp", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/151205/items/KE23B7G9", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/uw-madison_msp/items/KE23B7G9", "type": "text/html" } }, "meta": { "createdByUser": { "id": 978886, "username": "schmidli", "name": "Laura Schmidli", "links": { "alternate": { "href": "https://www.zotero.org/schmidli", "type": "text/html" } } }, "creatorSummary": "Vanderpool et al.", "parsedDate": "2013", "numChildren": 4 }, "data": { "key": "KE23B7G9", "version": 41, "itemType": "journalArticle", "title": "Patchy deletion of Bmpr1a potentiates proximal pulmonary artery remodeling in mice exposed to chronic hypoxia", "creators": [ { "creatorType": "author", "firstName": "Rebecca R", "lastName": "Vanderpool" }, { "creatorType": "author", "firstName": "Nesrine", "lastName": "El-Bizri" }, { "creatorType": "author", "firstName": "Marlene", "lastName": "Rabinovitch" }, { "creatorType": "author", "firstName": "Naomi C", "lastName": "Chesler" } ], "abstractNote": "Reduced vascular expression of bone morphogenetic protein type IA receptor (Bmpr1a) has been found in patients with pulmonary arterial hypertension. Our previous studies in mice with patchy deletion of Bmpr1a in vascular smooth muscle cells and cardiac myocytes showed decreased distal vascular remodeling despite a similar severity of hypoxic pulmonary hypertension (HPH). We speculate increased stiffness from ectopic deposition of collagen in proximal pulmonary arteries might account for HPH. Pulsatile pressure-flow relationships were measured in isolated, ventilated, perfused lungs of SM22α;TRE-Cre;R26R;Bmpr1a(flox/flox) (KO) mice and wild-type littermates, following 21 days (hypoxia) and 0 days (control) of chronic hypoxia. Pulmonary vascular impedance, which yields insight into proximal and distal arterial remodeling, was calculated. Reduced Bmpr1a expression had no effect on input impedance Z(0) (P = 0.52) or characteristic impedance Z(C) (P = 0.18) under control conditions; it also had no effect on the decrease in Z(0) via acute rho kinase inhibition. However, following chronic hypoxia, reduced Bmpr1a expression increased Z(C) (P < 0.001) without affecting Z(0) (P = 0.72). These results demonstrate that Bmpr1a deficiency does not significantly alter the hemodynamic function of the distal vasculature or its response to chronic hypoxia but larger, more proximal arteries are affected. In particular, reduced Bmpr1a expression likely decreased dilatation and increased stiffening in response to hypoxia, probably by collagen accumulation. Increased PA stiffness can have a significant impact on right ventricular function. This study illustrates for the first time how proximal pulmonary artery changes in the absence of distal pulmonary artery changes contribute to pulmonary arterial hypertension.", "publicationTitle": "Biomechanics and Modeling in Mechanobiology", "publisher": "", "place": "", "date": "2013", "volume": "12", "issue": "1", "section": "", "partNumber": "", "partTitle": "", "pages": "33-42", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "Biomech Model Mechanobiol", "DOI": "10.1007/s10237-012-0379-6", "citationKey": "", "url": "", "accessDate": "", "PMID": "22314711", "PMCID": "", "ISSN": "1617-7940", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "eng", "libraryCatalog": "NCBI PubMed", "callNumber": "", "rights": "", "extra": "", "tags": [ { "tag": "Animals", "type": 1 }, { "tag": "Anoxia", "type": 1 }, { "tag": "Bone Morphogenetic Protein Receptors, Type I", "type": 1 }, { "tag": "Computer Simulation", "type": 1 }, { "tag": "Elastic Modulus", "type": 1 }, { "tag": "Female", "type": 1 }, { "tag": "Male", "type": 1 }, { "tag": "Mechanotransduction, Cellular", "type": 1 }, { "tag": "Mice", "type": 1 }, { "tag": "Mice, Knockout", "type": 1 }, { "tag": "Models, Cardiovascular", "type": 1 }, { "tag": "Pulmonary Artery", "type": 1 }, { "tag": "Vascular Resistance", "type": 1 } ], "collections": [ "DKSEB5FD" ], "relations": { "dc:replaces": [ "http://zotero.org/groups/151205/items/RBKD3NGH", "http://zotero.org/groups/151205/items/9X7XHI64" ] }, "dateAdded": "2013-07-17T19:39:51Z", "dateModified": "2013-07-17T19:55:18Z" } }, { "key": "CAE754TA", "version": 40, "library": { "type": "group", "id": 151205, "name": "UW-Madison MSP", "links": { "alternate": { "href": "https://www.zotero.org/groups/uw-madison_msp", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/151205/items/CAE754TA", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/uw-madison_msp/items/CAE754TA", "type": "text/html" }, "up": { "href": "https://api.zotero.org/groups/151205/items/KE23B7G9", "type": "application/json" } }, "meta": { "createdByUser": { "id": 978886, "username": "schmidli", "name": "Laura Schmidli", "links": { "alternate": { "href": "https://www.zotero.org/schmidli", "type": "text/html" } } }, "numChildren": 0 }, "data": { "key": "CAE754TA", "version": 40, "parentItem": "KE23B7G9", "itemType": "note", "note": "PT: J; TC: 1; UT: WOS:000313480100004
", "tags": [], "relations": {}, "dateAdded": "2013-06-28T20:31:26Z", "dateModified": "2013-07-17T19:55:18Z" } }, { "key": "B44VRIDA", "version": 40, "library": { "type": "group", "id": 151205, "name": "UW-Madison MSP", "links": { "alternate": { "href": "https://www.zotero.org/groups/uw-madison_msp", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/151205/items/B44VRIDA", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/uw-madison_msp/items/B44VRIDA", "type": "text/html" }, "up": { "href": "https://api.zotero.org/groups/151205/items/KE23B7G9", "type": "application/json" } }, "meta": { "createdByUser": { "id": 978886, "username": "schmidli", "name": "Laura Schmidli", "links": { "alternate": { "href": "https://www.zotero.org/schmidli", "type": "text/html" } } }, "numChildren": 0 }, "data": { "key": "B44VRIDA", "version": 40, "parentItem": "KE23B7G9", "itemType": "note", "note": "The following values have no corresponding Zotero field:JID: 101135325; 2011/08/12 [received]; 2012/01/23 [accepted]; 2012/02/08 [aheadofprint]; aheadofprint
", "tags": [], "relations": {}, "dateAdded": "2013-06-28T20:27:40Z", "dateModified": "2013-07-17T19:55:18Z" } }, { "key": "D2AFGD9P", "version": 40, "library": { "type": "group", "id": 151205, "name": "UW-Madison MSP", "links": { "alternate": { "href": "https://www.zotero.org/groups/uw-madison_msp", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/151205/items/D2AFGD9P", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/uw-madison_msp/items/D2AFGD9P", "type": "text/html" } }, "meta": { "createdByUser": { "id": 978886, "username": "schmidli", "name": "Laura Schmidli", "links": { "alternate": { "href": "https://www.zotero.org/schmidli", "type": "text/html" } } }, "creatorSummary": "Dimalanta et al.", "parsedDate": "2004", "numChildren": 0 }, "data": { "key": "D2AFGD9P", "version": 40, "itemType": "journalArticle", "title": "A microfluidic system for large DNA molecule arrays", "creators": [ { "creatorType": "author", "firstName": "E. T.", "lastName": "Dimalanta" }, { "creatorType": "author", "firstName": "A.", "lastName": "Lim" }, { "creatorType": "author", "firstName": "R. R.", "lastName": "Runnheim" }, { "creatorType": "author", "firstName": "C.", "lastName": "Lamers" }, { "creatorType": "author", "firstName": "C.", "lastName": "Churas" }, { "creatorType": "author", "firstName": "D. K.", "lastName": "Forrest" }, { "creatorType": "author", "firstName": "J. J.", "lastName": "dePablo" }, { "creatorType": "author", "firstName": "M.", "lastName": "Graham" }, { "creatorType": "author", "firstName": "S. N.", "lastName": "Coppersmith" }, { "creatorType": "author", "firstName": "D. C.", "lastName": "Schwartz" } ], "abstractNote": "", "publicationTitle": "Analytical Chemistry", "publisher": "", "place": "", "date": "2004", "volume": "76", "issue": "", "section": "", "partNumber": "", "partTitle": "", "pages": "5293-5301", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "", "DOI": "", "citationKey": "", "url": "", "accessDate": "", "PMID": "", "PMCID": "", "ISSN": "", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "", "libraryCatalog": "", "callNumber": "", "rights": "", "extra": "Journal Article", "tags": [], "collections": [ "SQU52PJN" ], "relations": {}, "dateAdded": "2013-06-28T17:59:57Z", "dateModified": "2013-07-17T19:54:12Z" } }, { "key": "CX2X5TP7", "version": 40, "library": { "type": "group", "id": 151205, "name": "UW-Madison MSP", "links": { "alternate": { "href": "https://www.zotero.org/groups/uw-madison_msp", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/151205/items/CX2X5TP7", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/uw-madison_msp/items/CX2X5TP7", "type": "text/html" } }, "meta": { "createdByUser": { "id": 978886, "username": "schmidli", "name": "Laura Schmidli", "links": { "alternate": { "href": "https://www.zotero.org/schmidli", "type": "text/html" } } }, "creatorSummary": "Tikoff and Blanquat", "parsedDate": "1997", "numChildren": 1 }, "data": { "key": "CX2X5TP7", "version": 40, "itemType": "journalArticle", "title": "Transpressional shearing and strike-slip partitioning in the Late Cretaceous Sierra Nevada magmatic arc, California", "creators": [ { "creatorType": "author", "firstName": "Basil", "lastName": "Tikoff" }, { "creatorType": "author", "firstName": "Michel de Saint", "lastName": "Blanquat" } ], "abstractNote": "The Rosy Finch shear zone (RFSZ) represents an example of synmagmatic, strike-slip tectonics in the east central Sierra Nevada and provides information on transpressional tectonics within magmatic arcs. The RFSZ (1-4 km wide and 80 km long) is contained in Late Cretaceous granitoids (92?83 Ma) of the Mono Pass Intrusive Suite. Dextral strike-slip deformation is indicated by a continuous band of orthogneiss, mylonitic, and cataclastic deformation, characterized by subvertical foliations with subhorizontal lineations. The style of dextral shearing in the Rosy Finch shear varies along strike, from wide zones of ductile deformation in the youngest plutons to narrow zones of cataclastic deformation in the older plutons. Deformation of the youngest granitoids is synmagmatic, as constrained by both field observations and isotopic studies. Dextral shearing occurs concurrently in contemporaneous Intrusive Suites to the north and south (Tuolumne and Mount Whitney) suggesting strike-slip movement along the axis of active plutonism. Field studies also suggest that contraction across the arc acted concurrently with strike-slip movement, consistent with a transpressional setting for the Late Cretaceous Sierra Nevada magroatic arc. Comparison of the field data with strain models of oblique plate convergence suggests that the RFSZ is a preserved ductile signature of transcurrent motion of strike-slip partitioning within a transpressional orogenic system. Recent plate reconstructions indicate a switch from sinistral to dextral oblique convergence at ?95 Ma, which is consistent with the timing constraints on the dextral movement on the RFSZ.", "publicationTitle": "Tectonics", "publisher": "", "place": "", "date": "1997", "volume": "16", "issue": "3", "section": "", "partNumber": "", "partTitle": "", "pages": "PAGES 442?459-PAGES 442?459", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "Tectonics", "DOI": "", "citationKey": "", "url": "http://www.agu.org/pubs/crossref/1997/97TC00720.shtml", "accessDate": "", "PMID": "", "PMCID": "", "ISSN": "", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "", "libraryCatalog": "", "callNumber": "", "rights": "", "extra": "Journal Article", "tags": [], "collections": [ "SQU52PJN" ], "relations": {}, "dateAdded": "2013-06-28T17:59:57Z", "dateModified": "2013-07-17T19:53:41Z" } }, { "key": "HFXS979C", "version": 46, "library": { "type": "group", "id": 151205, "name": "UW-Madison MSP", "links": { "alternate": { "href": "https://www.zotero.org/groups/uw-madison_msp", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/151205/items/HFXS979C", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/uw-madison_msp/items/HFXS979C", "type": "text/html" } }, "meta": { "createdByUser": { "id": 978886, "username": "schmidli", "name": "Laura Schmidli", "links": { "alternate": { "href": "https://www.zotero.org/schmidli", "type": "text/html" } } }, "creatorSummary": "Su et al.", "parsedDate": "2012", "numChildren": 1 }, "data": { "key": "HFXS979C", "version": 46, "itemType": "journalArticle", "title": "Functional screen of paracrine signals in breast carcinoma fibroblasts", "creators": [ { "creatorType": "author", "firstName": "Gui", "lastName": "Su" }, { "creatorType": "author", "firstName": "Kyung E", "lastName": "Sung" }, { "creatorType": "author", "firstName": "David J", "lastName": "Beebe" }, { "creatorType": "author", "firstName": "Andreas", "lastName": "Friedl" } ], "abstractNote": "Stromal fibroblasts actively participate in normal mammary gland homeostasis and in breast carcinoma growth and progression by secreting paracrine factors; however, little is known about the identity of paracrine mediators in individual patients. The purpose of this study was to characterize paracrine signaling pathways between breast carcinoma cells and breast carcinoma-associated fibroblasts (CAF) or normal mammary fibroblasts (NF), respectively. CAF and NF were isolated from breast carcinoma tissue samples and adjacent normal mammary gland tissue of 28 patients. The fibroblasts were grown in 3D collagen gel co-culture with T47D human breast carcinoma cells and T47D cell growth was measured. CAF stimulated T47D cell growth to a significantly greater degree than NF. We detected a considerable inter-individual heterogeneity of paracrine interactions but identified FGF2, HB-EGF, heparanase-1 and SDF1 as factors that were consistently responsible for the activity of carcinoma-associated fibroblasts. CAF from low-grade but not high-grade carcinomas required insulin-like growth factor 1 and transforming growth factor beta 1 to stimulate carcinoma growth. Paradoxically, blocking of membrane-type 1 matrix metalloprotease stimulated T47D cell growth in co-culture with NF. The results were largely mirrored by treating the fibroblasts with siRNA oligonucleotides prior to co-culture, implicating the fibroblasts as principal production site for the secreted mediators. In summary, we identify a paracrine signaling network with inter-individual commonalities and differences. These findings have significant implications for the design of stroma-targeted therapies.", "publicationTitle": "PloS one", "publisher": "", "place": "", "date": "2012", "volume": "7", "issue": "10", "section": "", "partNumber": "", "partTitle": "", "pages": "e46685", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "PLoS ONE", "DOI": "10.1371/journal.pone.0046685", "citationKey": "", "url": "", "accessDate": "", "PMID": "23056402", "PMCID": "", "ISSN": "1932-6203", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "eng", "libraryCatalog": "NCBI PubMed", "callNumber": "", "rights": "", "extra": "", "tags": [ { "tag": "Breast", "type": 1 }, { "tag": "Breast Neoplasms", "type": 1 }, { "tag": "Cell Line, Tumor", "type": 1 }, { "tag": "Cells, Cultured", "type": 1 }, { "tag": "Coculture Techniques", "type": 1 }, { "tag": "Female", "type": 1 }, { "tag": "Fibroblasts", "type": 1 }, { "tag": "Humans", "type": 1 }, { "tag": "Paracrine Communication", "type": 1 }, { "tag": "Tumor Cells, Cultured", "type": 1 } ], "collections": [ "NAMDWKK8" ], "relations": {}, "dateAdded": "2013-07-17T19:39:51Z", "dateModified": "2013-07-17T19:39:51Z" } }, { "key": "TKMMME8D", "version": 46, "library": { "type": "group", "id": 151205, "name": "UW-Madison MSP", "links": { "alternate": { "href": "https://www.zotero.org/groups/uw-madison_msp", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/151205/items/TKMMME8D", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/uw-madison_msp/items/TKMMME8D", "type": "text/html" } }, "meta": { "createdByUser": { "id": 978886, "username": "schmidli", "name": "Laura Schmidli", "links": { "alternate": { "href": "https://www.zotero.org/schmidli", "type": "text/html" } } }, "creatorSummary": "Hong et al.", "parsedDate": "2013", "numChildren": 1 }, "data": { "key": "TKMMME8D", "version": 46, "itemType": "journalArticle", "title": "HaloTag as a reporter gene: positron emission tomography imaging with (64)Cu-labeled second generation HaloTag ligands", "creators": [ { "creatorType": "author", "firstName": "Hao", "lastName": "Hong" }, { "creatorType": "author", "firstName": "Hélène A", "lastName": "Benink" }, { "creatorType": "author", "firstName": "H Tetsuo", "lastName": "Uyeda" }, { "creatorType": "author", "firstName": "Hector F", "lastName": "Valdovinos" }, { "creatorType": "author", "firstName": "Yin", "lastName": "Zhang" }, { "creatorType": "author", "firstName": "Poncho", "lastName": "Meisenheimer" }, { "creatorType": "author", "firstName": "Todd E", "lastName": "Barnhart" }, { "creatorType": "author", "firstName": "Frank", "lastName": "Fan" }, { "creatorType": "author", "firstName": "Weibo", "lastName": "Cai" } ], "abstractNote": "THE GOAL OF THIS STUDY IS TO EMPLOY THE HALOTAG TECHNOLOGY FOR POSITRON EMISSION TOMOGRAPHY (PET), WHICH INVOLVES TWO COMPONENTS: the HaloTag protein (a modified hydrolase which covalently binds to synthetic ligands) and HaloTag ligands (HTLs). 4T1 murine breast cancer cells were stably transfected to express HaloTag protein on the surface (termed as 4T1-HaloTag-ECS, ECS denotes extracellular surface). Two new HTLs were synthesized and termed NOTA-HTL2G-S and NOTA-HTL2G-L (2G indicates second generation, S stands for short, L stands for long, NOTA denotes 1,4,7-triazacyclononane-N,N'N''-triacetic acid). Microscopy studies confirmed surface expression of HaloTag in 4T1-HaloTag-ECS cells, which specifically bind NOTA-HTL2G-S/L. Uptake of (64)Cu-NOTA-HTL2G-L in 4T1-HaloTag-ECS tumors (4.3 ± 0.5, 4.1± 0.2, 4.0 ± 0.2, 2.3 ± 0.1, and 2.2 ± 0.1 %ID/g at 0.5, 3, 6, 18, and 24 h post-injection respectively; n = 4) was significantly higher than that in the 4T1 tumors (3.0 ± 0.3, 3.0± 0.1, 3.0 ± 0.2, 2.0 ± 0.4, and 2.4 ± 0.3 %ID/g at 0.5, 3, 6, 18, and 24 h post-injection respectively; n = 4) at early time points. In comparison, (64)Cu-NOTA-HTL2G-S did not demonstrate significant uptake in either 4T1-HaloTag-ECS or 4T1 tumors. Blocking studies and autoradiography of tumor lysates confirmed that (64)Cu-NOTA-HTL2G-L binds specifically to HaloTag protein in the 4T1-HaloTag-ECS tumors, corroborated by histology. HaloTag protein-specific targeting and PET imaging in vivo with (64)Cu-NOTA-HTL2G-L serves as a proof-of-principle for future non-invasive and sensitive tracking of HaloTag-transfected cells with PET, as well as many other studies of gene/protein/cell function in vivo.", "publicationTitle": "American journal of translational research", "publisher": "", "place": "", "date": "2013", "volume": "5", "issue": "3", "section": "", "partNumber": "", "partTitle": "", "pages": "291-302", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "Am J Transl Res", "DOI": "", "citationKey": "", "url": "", "accessDate": "", "PMID": "23634240", "PMCID": "", "ISSN": "1943-8141", "archive": "", "archiveLocation": "", "shortTitle": "HaloTag as a reporter gene", "language": "eng", "libraryCatalog": "NCBI PubMed", "callNumber": "", "rights": "", "extra": "", "tags": [], "collections": [ "DKSEB5FD" ], "relations": {}, "dateAdded": "2013-07-17T19:39:51Z", "dateModified": "2013-07-17T19:39:51Z" } }, { "key": "W3K6SQRM", "version": 45, "library": { "type": "group", "id": 151205, "name": "UW-Madison MSP", "links": { "alternate": { "href": "https://www.zotero.org/groups/uw-madison_msp", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/151205/items/W3K6SQRM", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/uw-madison_msp/items/W3K6SQRM", "type": "text/html" } }, "meta": { "createdByUser": { "id": 978886, "username": "schmidli", "name": "Laura Schmidli", "links": { "alternate": { "href": "https://www.zotero.org/schmidli", "type": "text/html" } } }, "creatorSummary": "Nelson et al.", "parsedDate": "2013-06", "numChildren": 1 }, "data": { "key": "W3K6SQRM", "version": 45, "itemType": "journalArticle", "title": "Development of concept-based physiology lessons for biomedical engineering undergraduate students", "creators": [ { "creatorType": "author", "firstName": "Regina K", "lastName": "Nelson" }, { "creatorType": "author", "firstName": "Naomi C", "lastName": "Chesler" }, { "creatorType": "author", "firstName": "Kevin T", "lastName": "Strang" } ], "abstractNote": "Physiology is a core requirement in the undergraduate biomedical engineering curriculum. In one or two introductory physiology courses, engineering students must learn physiology sufficiently to support learning in their subsequent engineering courses and careers. As preparation for future learning, physiology instruction centered on concepts may help engineering students to further develop their physiology and biomedical engineering knowledge. Following the Backward Design instructional model, a series of seven concept-based lessons was developed for undergraduate engineering students. These online lessons were created as prerequisite physiology training to prepare students to engage in a collaborative engineering challenge activity. This work is presented as an example of how to convert standard, organ system-based physiology content into concept-based content lessons.", "publicationTitle": "Advances in physiology education", "publisher": "", "place": "", "date": "Jun 2013", "volume": "37", "issue": "2", "section": "", "partNumber": "", "partTitle": "", "pages": "176-183", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "Adv Physiol Educ", "DOI": "10.1152/advan.00038.2012", "citationKey": "", "url": "", "accessDate": "", "PMID": "23728135", "PMCID": "", "ISSN": "1522-1229", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "eng", "libraryCatalog": "NCBI PubMed", "callNumber": "", "rights": "", "extra": "", "tags": [], "collections": [ "DKSEB5FD" ], "relations": {}, "dateAdded": "2013-07-17T19:39:51Z", "dateModified": "2013-07-17T19:39:51Z" } }, { "key": "NUUJTBEC", "version": 45, "library": { "type": "group", "id": 151205, "name": "UW-Madison MSP", "links": { "alternate": { "href": "https://www.zotero.org/groups/uw-madison_msp", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/151205/items/NUUJTBEC", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/uw-madison_msp/items/NUUJTBEC", "type": "text/html" } }, "meta": { "createdByUser": { "id": 978886, "username": "schmidli", "name": "Laura Schmidli", "links": { "alternate": { "href": "https://www.zotero.org/schmidli", "type": "text/html" } } }, "creatorSummary": "Wang et al.", "parsedDate": "2013-06-06", "numChildren": 1 }, "data": { "key": "NUUJTBEC", "version": 45, "itemType": "journalArticle", "title": "Spontaneous Phase Transformation and Exfoliation of Rectangular Single-Crystal Zinc Hydroxy Dodecylsulfate Nanomembranes", "creators": [ { "creatorType": "author", "firstName": "Fei", "lastName": "Wang" }, { "creatorType": "author", "firstName": "Joseph E", "lastName": "Jakes" }, { "creatorType": "author", "firstName": "Dalong", "lastName": "Geng" }, { "creatorType": "author", "firstName": "Xudong", "lastName": "Wang" } ], "abstractNote": "Free-standing two-dimensional (2D) nanostructures, exemplified by graphene and semiconductor nanomembranes, exhibit exotic electrical and mechanical properties and have great potential in electronic applications where devices need to be flexible or conformal to nonplanar surfaces. Based on our previous development of a substrate-free synthesis of large-area, free-standing zinc hydroxy dodecylsulfate (ZHDS) hexagonal nanomembranes, herein, we report a spontaneous phase transformation of ZHDS nanomembranes under extended reaction time. The hexagonal ZHDS sheets transformed into rectangular single crystal nanomembranes with sizes of hundreds of micrometers. They contain long-range-ordered zinc vacancies that can be fitted into an orthorhombic superlattice. A surplus of dodecylsulfate ions and a deficit of Zn(2+) diffusion near the water surface are believed to be the factors that drive the phase transformation. The phase transformation starts with the formation of zinc vacancies at the topmost layer of the hexagonal hillock, and propagates along the spiral growth path of the initial hexagonal sheets, which bears a great resemblance to the classic \"periodic slip process\". Mechanical property characterization of ZHDS nanomembranes by nanoindentation shows they behave much like structural polymers mechanically due to the incorporation of surfactant molecules. We also developed a one-step exfoliation and dehydration method that converts ZHDS nanomembranes to ZnO nanosheets using n-butylamine. This work provides a further understanding of the growth and stability of ZnO-based nanomembranes, as well as advisory insight for the further development on solution-based synthesis of free-standing, single-crystalline 2D nanostructures.", "publicationTitle": "ACS nano", "publisher": "", "place": "", "date": "Jun 6, 2013", "volume": "", "issue": "", "section": "", "partNumber": "", "partTitle": "", "pages": "", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "ACS Nano", "DOI": "10.1021/nn4017108", "citationKey": "", "url": "", "accessDate": "", "PMID": "23730895", "PMCID": "", "ISSN": "1936-086X", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "ENG", "libraryCatalog": "NCBI PubMed", "callNumber": "", "rights": "", "extra": "", "tags": [], "collections": [ "DKSEB5FD" ], "relations": {}, "dateAdded": "2013-07-17T19:39:51Z", "dateModified": "2013-07-17T19:39:51Z" } }, { "key": "77XNS3ST", "version": 45, "library": { "type": "group", "id": 151205, "name": "UW-Madison MSP", "links": { "alternate": { "href": "https://www.zotero.org/groups/uw-madison_msp", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/151205/items/77XNS3ST", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/uw-madison_msp/items/77XNS3ST", "type": "text/html" } }, "meta": { "createdByUser": { "id": 978886, "username": "schmidli", "name": "Laura Schmidli", "links": { "alternate": { "href": "https://www.zotero.org/schmidli", "type": "text/html" } } }, "creatorSummary": "Eun et al.", "parsedDate": "2013-07-03", "numChildren": 1 }, "data": { "key": "77XNS3ST", "version": 45, "itemType": "journalArticle", "title": "Divin: a small molecule inhibitor of bacterial divisome assembly", "creators": [ { "creatorType": "author", "firstName": "Ye-Jin", "lastName": "Eun" }, { "creatorType": "author", "firstName": "Maoquan", "lastName": "Zhou" }, { "creatorType": "author", "firstName": "Daniela", "lastName": "Kiekebusch" }, { "creatorType": "author", "firstName": "Susan", "lastName": "Schlimpert" }, { "creatorType": "author", "firstName": "Rishi R", "lastName": "Trivedi" }, { "creatorType": "author", "firstName": "Somenath", "lastName": "Bakshi" }, { "creatorType": "author", "firstName": "Zhou", "lastName": "Zhong" }, { "creatorType": "author", "firstName": "Taylor A", "lastName": "Wahlig" }, { "creatorType": "author", "firstName": "Martin", "lastName": "Thanbichler" }, { "creatorType": "author", "firstName": "Douglas B", "lastName": "Weibel" } ], "abstractNote": "Bacterial cell division involves the dynamic assembly of division proteins and coordinated constriction of the cell envelope. A wide range of factors regulates cell division-including growth and environmental stresses-and the targeting of the division machinery has been a widely discussed approach for antimicrobial therapies. This paper introduces divin, a small molecule inhibitor of bacterial cell division that may facilitate mechanistic studies of this process. Divin disrupts the assembly of late division proteins, reduces peptidoglycan remodeling at the division site, and blocks compartmentalization of the cytoplasm. In contrast to other division inhibitors, divin does not interact with the tubulin homologue FtsZ, affect chromosome segregation, or activate regulatory mechanisms that inhibit cell division indirectly. Our studies of bacterial cell division using divin as a probe suggest that dividing bacteria proceed through several morphological stages of the cell envelope, and FtsZ is required but not sufficient to compartmentalize the cytoplasmic membrane at the division site. Divin is only moderately toxic to mammalian cells at concentrations that inhibit the growth of clinical pathogens. These characteristics make divin a useful probe for studying bacterial cell division and a starting point for the development of new classes of therapeutic agents.", "publicationTitle": "Journal of the American Chemical Society", "publisher": "", "place": "", "date": "Jul 3, 2013", "volume": "135", "issue": "26", "section": "", "partNumber": "", "partTitle": "", "pages": "9768-9776", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "J. Am. Chem. Soc.", "DOI": "10.1021/ja404640f", "citationKey": "", "url": "", "accessDate": "", "PMID": "23738839", "PMCID": "", "ISSN": "1520-5126", "archive": "", "archiveLocation": "", "shortTitle": "Divin", "language": "eng", "libraryCatalog": "NCBI PubMed", "callNumber": "", "rights": "", "extra": "", "tags": [], "collections": [ "DKSEB5FD" ], "relations": {}, "dateAdded": "2013-07-17T19:39:51Z", "dateModified": "2013-07-17T19:39:51Z" } }, { "key": "DBNC22MT", "version": 45, "library": { "type": "group", "id": 151205, "name": "UW-Madison MSP", "links": { "alternate": { "href": "https://www.zotero.org/groups/uw-madison_msp", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/151205/items/DBNC22MT", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/uw-madison_msp/items/DBNC22MT", "type": "text/html" } }, "meta": { "createdByUser": { "id": 978886, "username": "schmidli", "name": "Laura Schmidli", "links": { "alternate": { "href": "https://www.zotero.org/schmidli", "type": "text/html" } } }, "creatorSummary": "Selekman et al.", "parsedDate": "2013-06-05", "numChildren": 1 }, "data": { "key": "DBNC22MT", "version": 45, "itemType": "journalArticle", "title": "Improving efficiency of human pluripotent stem cell differentiation platforms using an integrated experimental and computational approach", "creators": [ { "creatorType": "author", "firstName": "Joshua A", "lastName": "Selekman" }, { "creatorType": "author", "firstName": "Amritava", "lastName": "Das" }, { "creatorType": "author", "firstName": "Nicholas J", "lastName": "Grundl" }, { "creatorType": "author", "firstName": "Sean P", "lastName": "Palecek" } ], "abstractNote": "Human pluripotent stem cells (hPSCs) have an unparalleled potential for tissue engineering applications including regenerative therapies and in vitro cell-based models for studying normal and diseased tissue morphogenesis, or drug and toxicological screens. While numerous hPSC differentiation methods have been developed to generate various somatic cell types, the potential of hPSC-based technologies is hinged on the ability to translate these established lab-scale differentiation systems to large-scale processes to meet the industrial and clinical demands for these somatic cell types. Here, we demonstrate a strategy for investigating the efficiency and scalability of hPSC differentiation platforms. Using two previously reported epithelial differentiation systems as models, we fit an ODE-based kinetic model to data representing dynamics of various cell subpopulations present in our culture. This fit was performed by estimating rate constants of each cell subpopulation's cell fate decisions (self-renewal, differentiation, death). Sensitivity analyses on predicted rate constants indicated which cell fate decisions had the greatest impact on overall epithelial cell yield in each differentiation process. In addition, we found that the final cell yield was limited by the self-renewal rate of either the progenitor state or the final differentiated state, depending on the differentiation protocol. Also, the relative impact of these cell fate decision rates was highly dependent on the maximum capacity of the cell culture system. Overall, we outline a novel approach for quantitative analysis of established laboratory-scale hPSC differentiation systems and this approach may ease development to produce large quantities of cells for tissue engineering applications. Biotechnol. Bioeng. 2013;9999: 1-14. © 2013 Wiley Periodicals, Inc.", "publicationTitle": "Biotechnology and bioengineering", "publisher": "", "place": "", "date": "Jun 5, 2013", "volume": "", "issue": "", "section": "", "partNumber": "", "partTitle": "", "pages": "", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "Biotechnol. Bioeng.", "DOI": "10.1002/bit.24968", "citationKey": "", "url": "", "accessDate": "", "PMID": "23740478", "PMCID": "", "ISSN": "1097-0290", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "ENG", "libraryCatalog": "NCBI PubMed", "callNumber": "", "rights": "", "extra": "", "tags": [], "collections": [ "DKSEB5FD" ], "relations": {}, "dateAdded": "2013-07-17T19:39:51Z", "dateModified": "2013-07-17T19:39:51Z" } }, { "key": "PIUSTBRJ", "version": 45, "library": { "type": "group", "id": 151205, "name": "UW-Madison MSP", "links": { "alternate": { "href": "https://www.zotero.org/groups/uw-madison_msp", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/151205/items/PIUSTBRJ", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/uw-madison_msp/items/PIUSTBRJ", "type": "text/html" } }, "meta": { "createdByUser": { "id": 978886, "username": "schmidli", "name": "Laura Schmidli", "links": { "alternate": { "href": "https://www.zotero.org/schmidli", "type": "text/html" } } }, "creatorSummary": "Zhang et al.", "parsedDate": "2013-06-06", "numChildren": 1 }, "data": { "key": "PIUSTBRJ", "version": 45, "itemType": "journalArticle", "title": "Dye-Sensitized Solar Cell with Energy Storage Function through PVDF/ZnO Nanocomposite Counter Electrode", "creators": [ { "creatorType": "author", "firstName": "Xi", "lastName": "Zhang" }, { "creatorType": "author", "firstName": "Xuezhen", "lastName": "Huang" }, { "creatorType": "author", "firstName": "Chensha", "lastName": "Li" }, { "creatorType": "author", "firstName": "Hongrui", "lastName": "Jiang" } ], "abstractNote": "Dye-sensitized solar cells with an energy storage function are demonstrated by modifying its counter electrode with a poly (vinylidene fluoride)/ZnO nanowire array composite. This simplex device could still function as an ordinary solar cell with a steady photocurrent output even after being fully charged. An energy storage density of 2.14 C g(-1) is achieved, while simultaneously a 3.70% photo-to-electric conversion efficiency is maintained.", "publicationTitle": "Advanced materials (Deerfield Beach, Fla.)", "publisher": "", "place": "", "date": "Jun 6, 2013", "volume": "", "issue": "", "section": "", "partNumber": "", "partTitle": "", "pages": "", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "Adv. Mater. Weinheim", "DOI": "10.1002/adma.201301088", "citationKey": "", "url": "", "accessDate": "", "PMID": "23740719", "PMCID": "", "ISSN": "1521-4095", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "ENG", "libraryCatalog": "NCBI PubMed", "callNumber": "", "rights": "", "extra": "", "tags": [], "collections": [ "DKSEB5FD" ], "relations": {}, "dateAdded": "2013-07-17T19:39:51Z", "dateModified": "2013-07-17T19:39:51Z" } }, { "key": "5BKIJDPC", "version": 45, "library": { "type": "group", "id": 151205, "name": "UW-Madison MSP", "links": { "alternate": { "href": "https://www.zotero.org/groups/uw-madison_msp", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/151205/items/5BKIJDPC", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/uw-madison_msp/items/5BKIJDPC", "type": "text/html" } }, "meta": { "createdByUser": { "id": 978886, "username": "schmidli", "name": "Laura Schmidli", "links": { "alternate": { "href": "https://www.zotero.org/schmidli", "type": "text/html" } } }, "creatorSummary": "Degrave et al.", "parsedDate": "2013-06-12", "numChildren": 1 }, "data": { "key": "5BKIJDPC", "version": 45, "itemType": "journalArticle", "title": "A General Method To Measure the Hall Effect in Nanowires: Examples of FeS2 and MnSi", "creators": [ { "creatorType": "author", "firstName": "John P", "lastName": "Degrave" }, { "creatorType": "author", "firstName": "Dong", "lastName": "Liang" }, { "creatorType": "author", "firstName": "Song", "lastName": "Jin" } ], "abstractNote": "We present a general methodology for measuring the Hall effect on nanostructures with one-dimensional (1D) nanowire morphology. Relying only on typical e-beam lithography, the methodology developed herein utilizes an angled electrode evaporation technique so that the nanowire itself is a shadow mask and an intimate sidewall contact can be formed for the Hall electrodes. A six-contact electrode scheme with offset transverse contacts is utilized that allows monitoring of both the longitudinal resistivity and the Hall resistivity which is extracted from the raw voltage from the transverse electrodes using an antisymmetrization procedure. Our method does not require the use of a highly engineered lithographic process to produce directly opposing Hall electrodes with a very small gap. Hall effect measurements on semiconducting iron pyrite (FeS2) nanowire devices are validated by comparing to Hall effect measurements in the conventional Hall geometry using FeS2 plate devices. This Hall effect measurement is further extended to MnSi nanowires, and the distinct anomalous Hall effect signature is identified for the first time in chiral magnetic MnSi nanowires, a significant step toward identifying the topological Hall effect due to skyrmions in chiral magnetic nanowires.", "publicationTitle": "Nano letters", "publisher": "", "place": "", "date": "Jun 12, 2013", "volume": "13", "issue": "6", "section": "", "partNumber": "", "partTitle": "", "pages": "2704-2709", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "Nano Lett.", "DOI": "10.1021/nl400875z", "citationKey": "", "url": "", "accessDate": "", "PMID": "23701294", "PMCID": "", "ISSN": "1530-6992", "archive": "", "archiveLocation": "", "shortTitle": "A General Method To Measure the Hall Effect in Nanowires", "language": "eng", "libraryCatalog": "NCBI PubMed", "callNumber": "", "rights": "", "extra": "", "tags": [], "collections": [ "DKSEB5FD" ], "relations": {}, "dateAdded": "2013-07-17T19:39:51Z", "dateModified": "2013-07-17T19:39:51Z" } }, { "key": "KWRKRZSS", "version": 45, "library": { "type": "group", "id": 151205, "name": "UW-Madison MSP", "links": { "alternate": { "href": "https://www.zotero.org/groups/uw-madison_msp", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/151205/items/KWRKRZSS", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/uw-madison_msp/items/KWRKRZSS", "type": "text/html" } }, "meta": { "createdByUser": { "id": 978886, "username": "schmidli", "name": "Laura Schmidli", "links": { "alternate": { "href": "https://www.zotero.org/schmidli", "type": "text/html" } } }, "creatorSummary": "Aytar et al.", "parsedDate": "2013-06-19", "numChildren": 1 }, "data": { "key": "KWRKRZSS", "version": 45, "itemType": "journalArticle", "title": "Redox-Based Control of the Transformation and Activation of siRNA Complexes in Extracellular Environments Using Ferrocenyl Lipids", "creators": [ { "creatorType": "author", "firstName": "Burcu S", "lastName": "Aytar" }, { "creatorType": "author", "firstName": "John P E", "lastName": "Muller" }, { "creatorType": "author", "firstName": "Yukishige", "lastName": "Kondo" }, { "creatorType": "author", "firstName": "Yeshayahu", "lastName": "Talmon" }, { "creatorType": "author", "firstName": "Nicholas L", "lastName": "Abbott" }, { "creatorType": "author", "firstName": "David M", "lastName": "Lynn" } ], "abstractNote": "We report physical characterization and biological evaluation of complexes of small interfering RNA (siRNA) formed using a cationic lipid [bis(11-ferrocenylundecyl)dimethylammonium bromide (BFDMA)] containing redox-active ferrocenyl groups at the end of each hydrophobic tail. We demonstrate that control over the redox state of BFDMA can be used to influence key physical properties and control the activities of lipoplexes formed using siRNA-based constructs. Specifically, lipoplexes of siRNA and reduced BFDMA lead to high levels of sequence-specific gene silencing in cells, but lipoplexes formed using oxidized BFDMA do not. Lipoplexes of oxidized BFDMA can be activated in situ to induce high levels of silencing by addition of a chemical reducing agent, demonstrating a basis for external control over the activation/delivery of siRNA in cellular environments. Differences in activity arise from the inability of oxidized BFDMA to promote efficient internalization of siRNA; these differences also correlated to significant differences in the nanostructures of these lipoplexes (determined by cryo-TEM) and their ζ potentials as a function of oxidation state. These results are considered in view of recent studies characterizing the nanostructures, properties, and behaviors of lipoplexes formed using BFDMA and macromolecular plasmid DNA. We find that several key structural features and aspects of redox control observed for lipoplexes of plasmid DNA are maintained in complexes formed using smaller and more rigid siRNA. The ability to transform BFDMA in complex media presents opportunities to exert control over the nanostructures and behaviors of siRNA lipoplexes in ways not possible using conventional lipids. The approaches reported here could thus prove useful in both fundamental and applied contexts.", "publicationTitle": "Journal of the American Chemical Society", "publisher": "", "place": "", "date": "Jun 19, 2013", "volume": "135", "issue": "24", "section": "", "partNumber": "", "partTitle": "", "pages": "9111-9120", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "J. Am. Chem. Soc.", "DOI": "10.1021/ja403546b", "citationKey": "", "url": "", "accessDate": "", "PMID": "23701636", "PMCID": "", "ISSN": "1520-5126", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "eng", "libraryCatalog": "NCBI PubMed", "callNumber": "", "rights": "", "extra": "", "tags": [], "collections": [ "DKSEB5FD" ], "relations": {}, "dateAdded": "2013-07-17T19:39:51Z", "dateModified": "2013-07-17T19:39:51Z" } }, { "key": "EXSU3HTG", "version": 45, "library": { "type": "group", "id": 151205, "name": "UW-Madison MSP", "links": { "alternate": { "href": "https://www.zotero.org/groups/uw-madison_msp", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/151205/items/EXSU3HTG", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/uw-madison_msp/items/EXSU3HTG", "type": "text/html" } }, "meta": { "createdByUser": { "id": 978886, "username": "schmidli", "name": "Laura Schmidli", "links": { "alternate": { "href": "https://www.zotero.org/schmidli", "type": "text/html" } } }, "creatorSummary": "Lu et al.", "parsedDate": "2012", "numChildren": 1 }, "data": { "key": "EXSU3HTG", "version": 45, "itemType": "journalArticle", "title": "Coating with a modular bone morphogenetic peptide promotes healing of a bone-implant gap in an ovine model", "creators": [ { "creatorType": "author", "firstName": "Yan", "lastName": "Lu" }, { "creatorType": "author", "firstName": "Jae Sung", "lastName": "Lee" }, { "creatorType": "author", "firstName": "Brett", "lastName": "Nemke" }, { "creatorType": "author", "firstName": "Ben K", "lastName": "Graf" }, { "creatorType": "author", "firstName": "Kevin", "lastName": "Royalty" }, { "creatorType": "author", "firstName": "Richard, 3rd", "lastName": "Illgen" }, { "creatorType": "author", "firstName": "Ray, Jr", "lastName": "Vanderby" }, { "creatorType": "author", "firstName": "Mark D", "lastName": "Markel" }, { "creatorType": "author", "firstName": "William L", "lastName": "Murphy" } ], "abstractNote": "Despite the potential for growth factor delivery strategies to promote orthopedic implant healing, there is a need for growth factor delivery methods that are controllable and amenable to clinical translation. We have developed a modular bone growth factor, herein termed \"modular bone morphogenetic peptide (mBMP)\", which was designed to efficiently bind to the surface of orthopedic implants and also stimulate new bone formation. The purpose of this study was to coat a hydroxyapatite-titanium implant with mBMP and evaluate bone healing across a bone-implant gap in the sheep femoral condyle. The mBMP molecules efficiently bound to a hydroxyapatite-titanium implant and 64% of the initially bound mBMP molecules were released in a sustained manner over 28 days. The results demonstrated that the mBMP-coated implant group had significantly more mineralized bone filling in the implant-bone gap than the control group in C-arm computed tomography (DynaCT) scanning (25% more), histological (35% more) and microradiographic images (50% more). Push-out stiffness of the mBMP group was nearly 40% greater than that of control group whereas peak force did not show a significant difference. The results of this study demonstrated that mBMP coated on a hydroxyapatite-titanium implant stimulates new bone formation and may be useful to improve implant fixation in total joint arthroplasty applications.", "publicationTitle": "PloS one", "publisher": "", "place": "", "date": "2012", "volume": "7", "issue": "11", "section": "", "partNumber": "", "partTitle": "", "pages": "e50378", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "PLoS ONE", "DOI": "10.1371/journal.pone.0050378", "citationKey": "", "url": "", "accessDate": "", "PMID": "23185610", "PMCID": "", "ISSN": "1932-6203", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "eng", "libraryCatalog": "NCBI PubMed", "callNumber": "", "rights": "", "extra": "", "tags": [ { "tag": "Animals", "type": 1 }, { "tag": "Bone Morphogenetic Proteins", "type": 1 }, { "tag": "Calcification, Physiologic", "type": 1 }, { "tag": "Coated Materials, Biocompatible", "type": 1 }, { "tag": "Cone-Beam Computed Tomography", "type": 1 }, { "tag": "Delayed-Action Preparations", "type": 1 }, { "tag": "Durapatite", "type": 1 }, { "tag": "Female", "type": 1 }, { "tag": "Femur", "type": 1 }, { "tag": "Osseointegration", "type": 1 }, { "tag": "Prostheses and Implants", "type": 1 }, { "tag": "Sheep", "type": 1 }, { "tag": "Sheep, Domestic", "type": 1 }, { "tag": "Tensile Strength", "type": 1 }, { "tag": "Titanium", "type": 1 }, { "tag": "Wound Healing", "type": 1 } ], "collections": [ "NAMDWKK8" ], "relations": {}, "dateAdded": "2013-07-17T19:39:51Z", "dateModified": "2013-07-17T19:39:51Z" } }, { "key": "CK7UPV26", "version": 45, "library": { "type": "group", "id": 151205, "name": "UW-Madison MSP", "links": { "alternate": { "href": "https://www.zotero.org/groups/uw-madison_msp", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/151205/items/CK7UPV26", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/uw-madison_msp/items/CK7UPV26", "type": "text/html" } }, "meta": { "createdByUser": { "id": 978886, "username": "schmidli", "name": "Laura Schmidli", "links": { "alternate": { "href": "https://www.zotero.org/schmidli", "type": "text/html" } } }, "creatorSummary": "Anderson et al.", "parsedDate": "2013-05-13", "numChildren": 1 }, "data": { "key": "CK7UPV26", "version": 45, "itemType": "journalArticle", "title": "A survey to examine patient awareness, knowledge, and perceptions regarding the risks and consequences of surgical site infections", "creators": [ { "creatorType": "author", "firstName": "Michael", "lastName": "Anderson" }, { "creatorType": "author", "firstName": "Andy", "lastName": "Ottum" }, { "creatorType": "author", "firstName": "Sara", "lastName": "Zerbel" }, { "creatorType": "author", "firstName": "Ajay", "lastName": "Sethi" }, { "creatorType": "author", "firstName": "Martha E", "lastName": "Gaines" }, { "creatorType": "author", "firstName": "Nasia", "lastName": "Safdar" } ], "abstractNote": "We surveyed patients to determine awareness and knowledge regarding risks and consequences of and prevention of surgical site infection (SSI), and we found that 26% of respondents thought that education for SSI prevention could be improved and that 16% could not recall discussing SSI risks and prevention with a health care worker at all. Only 60% of patients recalled receiving an informational flyer in the hospital. Our results show that better educational and engagement interventions incorporating patient preferences are needed to promote awareness and patient engagement regarding SSI prevention.", "publicationTitle": "American journal of infection control", "publisher": "", "place": "", "date": "May 13, 2013", "volume": "", "issue": "", "section": "", "partNumber": "", "partTitle": "", "pages": "", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "Am J Infect Control", "DOI": "10.1016/j.ajic.2013.02.007", "citationKey": "", "url": "", "accessDate": "", "PMID": "23680112", "PMCID": "", "ISSN": "1527-3296", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "ENG", "libraryCatalog": "NCBI PubMed", "callNumber": "", "rights": "", "extra": "", "tags": [], "collections": [ "DKSEB5FD" ], "relations": {}, "dateAdded": "2013-07-17T19:39:51Z", "dateModified": "2013-07-17T19:39:51Z" } }, { "key": "TRUMQRV9", "version": 45, "library": { "type": "group", "id": 151205, "name": "UW-Madison MSP", "links": { "alternate": { "href": "https://www.zotero.org/groups/uw-madison_msp", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/151205/items/TRUMQRV9", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/uw-madison_msp/items/TRUMQRV9", "type": "text/html" } }, "meta": { "createdByUser": { "id": 978886, "username": "schmidli", "name": "Laura Schmidli", "links": { "alternate": { "href": "https://www.zotero.org/schmidli", "type": "text/html" } } }, "creatorSummary": "Miller and Abbott", "parsedDate": "2013-01-14", "numChildren": 1 }, "data": { "key": "TRUMQRV9", "version": 45, "itemType": "journalArticle", "title": "Influence of droplet size, pH and ionic strength on endotoxin-triggered ordering transitions in liquid crystalline droplets", "creators": [ { "creatorType": "author", "firstName": "Daniel S", "lastName": "Miller" }, { "creatorType": "author", "firstName": "Nicholas L", "lastName": "Abbott" } ], "abstractNote": "We report an investigation of ordering transitions that are induced in water-dispersed, micrometer-sized droplets of a thermotropic liquid crystal (LC) by the bacterial lipopolysaccharide endotoxin. We reveal that the ordering transitions induced by endotoxin - from a bipolar state of the droplets to a radial state - are strongly dependent on the size of the LC droplets. Specifically, as the diameters of the LC droplets increase from 2 μm to above 10 μm (in phosphate buffered saline with an ionic strength of 90 mM and a pH of 7.2), we measured the percentage of droplets exhibiting a radial configuration in the presence of 100 pg/mL endotoxin to decrease from 98 ± 1 % to 3 ± 2 %. In addition, we measured a decrease in either the ionic strength or pH of the aqueous phase to reduce the percentage of droplets exhibiting a radial configuration in the presence of endotoxin. These results, when interpreted within the context of a simple thermodynamic model that incorporates the contributions of elasticity and surface anchoring to the free energies of the LC droplets, lead us to conclude that (i) the elastic constant K24 plays a central role in determining the size-dependent response of the LC droplets to endotoxin, and (ii) endotoxin-triggered ordering transitions occur only under solution conditions (pH, ionic strength) where the combined contributions of elasticity and surface anchoring to the free energies of the bipolar and radial configurations of the LC droplets are similar in magnitude. Our analysis also suggests that the presence of endotoxin perturbs the free energies of the LC droplets by ~10(-17) J/droplet, which is comparable to the standard free energy of self-association of ~10(3) endotoxin molecules. These results, when combined with prior reports of localization of endotoxin at the center of LC droplets, are consistent with the hypothesis that self-assembly of endotoxin within micrometer-sized LC droplets provides the driving force for the ordering transitions. Overall, these results advance our understanding of ordering transitions triggered by the interactions of analytes with LC droplets and, more broadly, provide guidance to the design of LC droplet systems as the basis of stimuli-responsive soft materials.", "publicationTitle": "Soft matter", "publisher": "", "place": "", "date": "Jan 14, 2013", "volume": "9", "issue": "2", "section": "", "partNumber": "", "partTitle": "", "pages": "374-382", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "Soft Matter", "DOI": "10.1039/C2SM26811F", "citationKey": "", "url": "", "accessDate": "", "PMID": "23675387", "PMCID": "", "ISSN": "1744-683X", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "ENG", "libraryCatalog": "NCBI PubMed", "callNumber": "", "rights": "", "extra": "", "tags": [], "collections": [ "DKSEB5FD" ], "relations": {}, "dateAdded": "2013-07-17T19:39:51Z", "dateModified": "2013-07-17T19:39:51Z" } } ]