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Cette affection neurodégénérative touche à la fois les motoneurones périphériques localisés dans la corne antérieure de la moëlle épinière ou des noyaux moteurs du bulbe et les neurones moteurs centraux du cortex cérébrale. Sa physiopathologie est imparfaitement élucidée. L'existence de formes familiales dans 5 à 10 % des cas a permis de réaliser des modèles animaux sur lesquels sont évalués les molécules neuroprotectrices potentielles avant le passage à l'homme. Sur le plan clinique, l'évolution est progressive marquée par des paralysies extensives qui conduisent au décés dans des délais variables entre 2 et 5 ans. Le profil évolutif est hétérogène et il possible de déterminer schématiquement trois profils différents : des patients déclineurs rapides (environ 25 %) dont la médiane de survie est inférieure à 12 mois, des patients déclineurs lents avec une durée d'évolution supérieure à 5 ans (25 %) voire 10 ans (10 %) et enfin des patients avec un profil classique mais hétérogène avec une durée d'évolution moyenne de 36 mois. Cette variabilité évolutive ne permet pas en pratique clinique de prédire le pronostic à long terme même si des facteurs pronostiques sont bien identifiés. Ce travail de thèse, a été réalisé dans le cadre d'une cotutelle entre l'Université de Limoges et l'Université de la Républica en Uruguay. L'objectif majeur était de débuter des travaux collaboratifs de neuroépidémiologie comparée dans deux régions du globe distinctes : une région située dans les zones tempérées de l'hémisphère Nord et un pays localisé en zone subtropicale de l'hémisphère Sud. La recherche s'est déroulée sur une période de 3 ans avec un séjour pendant 1 an en France puis des séjours de 3 mois. Ce travail de recherche épidémiologique comporte 4 études publiées pour 2 d'entre elles et 2 en soumission dans les revues internationales. Le premier travail de recherche est focalisé sur l'étude de l'incidence de la SLA en Limousin depuis 10 ans. Le deuxième travail a comparé les données épidémiologiques de la SLA en Uruguay avec le centre de référence SLA situé au CHU de Limoges sur 2000-2004. Le troisième travail multicentrique a analysé les circonstances et les causes de décès de 302 patients suivis en France dans 16 centres experts en 2006-2007. 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"https://api.zotero.org/groups/11183/items/BIQJFUW3", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/unilim_bu_sante/items/BIQJFUW3", "type": "text/html" } }, "meta": { "createdByUser": { "id": 61114, "username": "St_B", "name": "", "links": { "alternate": { "href": "https://www.zotero.org/st_b", "type": "text/html" } } }, "lastModifiedByUser": { "id": 108393, "username": "Iam01", "name": "", "links": { "alternate": { "href": "https://www.zotero.org/iam01", "type": "text/html" } } }, "creatorSummary": "Blitz and Nemes", "numChildren": 3 }, "data": { "key": "BIQJFUW3", "version": 4630, "itemType": "journalArticle", "title": "Bilateral Peroneus Longus Tendon Rupture Through a Bipartite Os Peroneum", "creators": [ { "creatorType": "author", "firstName": "Neal M.", "lastName": "Blitz" }, { "creatorType": "author", "firstName": "Kristine K.", "lastName": "Nemes" } ], "abstractNote": "Peroneus longus rupture with associated involvement of the os peroneum is an uncommon injury, and a small number of cases have been reported. Several mechanisms of injury have been suggested, but the most accepted theory is due to an inversion force placed on a cavovarus foot type. The sesamoid often becomes the focal point of the mechanical stresses and may fracture. Although the purpose of the sesamoid is to protect the tendon from rupture, the os peroneum may actually encourage fatigue (tear/rupture) under certain circumstances. Because this injury occurs at the cuboid notch, primary repair is complicated because of the inability to access the tendon as it courses deep within the midfoot. We present a bilateral case of peroneus longus rupture with involvement of the os peroneum in a patient with a cavovarus foot type. The injuries were sustained from an identical mechanism and occurred almost 1 year apart. In both situations, a peroneus longus to peroneus brevis tendon transfer was performed above the ankle joint in conjunction with partial excision of the fractured os peroneum. To the authors' knowledge, this is the only reported case of peroneus longus rupture associated with fracture of the os peroneum to occur bilaterally.", "publicationTitle": "The Journal of Foot and Ankle Surgery", "publisher": "", "place": "", "date": "Juillet", "volume": "46", "issue": "4", "section": "", "partNumber": "", "partTitle": "", "pages": "270-277", "series": "", "seriesTitle": "", "seriesText": "", "journalAbbreviation": "", "DOI": "10.1053/j.jfas.2007.03.006", "citationKey": "", "url": "http://www.sciencedirect.com/science/article/B75DJ-4P1DKDB-J/2/f82f4ba5c181c8cf425d4ed61ae4c0b8", "accessDate": "2010-02-04T16:52:56Z", "PMID": "", "PMCID": "", "ISSN": "1067-2516", "archive": "", "archiveLocation": "", "shortTitle": "", "language": "", "libraryCatalog": "ScienceDirect", "callNumber": "", "rights": "", "extra": "", "tags": [ { "tag": "bipartite", "type": 1 }, { "tag": "fracture", "type": 1 }, { "tag": "os peroneum", "type": 1 }, { "tag": "peroneus longus", "type": 1 }, { "tag": "tendon rupture", "type": 1 } ], "collections": [ "KA58HBQV" ], "relations": { "dc:replaces": "http://zotero.org/groups/11183/items/JEZGRV4U" }, "dateAdded": "2010-02-04T16:52:56Z", "dateModified": "2020-09-09T16:45:49Z" } }, { "key": "TSFCB6CJ", "version": 4622, "library": { "type": "group", "id": 11183, "name": "Unilim BU SANTE", "links": { "alternate": { "href": "https://www.zotero.org/groups/unilim_bu_sante", "type": "text/html" } } }, "links": { "self": { "href": "https://api.zotero.org/groups/11183/items/TSFCB6CJ", "type": "application/json" }, "alternate": { "href": "https://www.zotero.org/groups/unilim_bu_sante/items/TSFCB6CJ", "type": "text/html" }, "up": { "href": "https://api.zotero.org/groups/11183/items/7QW64IKK", "type": "application/json" } }, "meta": { "createdByUser": { "id": 108393, "username": "Iam01", "name": "", "links": { "alternate": { "href": "https://www.zotero.org/iam01", "type": "text/html" } } } }, "data": { "key": "TSFCB6CJ", "version": 4622, "parentItem": "7QW64IKK", "itemType": "attachment", "linkMode": "imported_url", "title": "Fleurs de Bach - 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} ], "abstractNote": "Reproduction de la thèse autorisée La multiplication et le développement des systèmes de communication sans fil ne se font pas sans provoquer de nombreuses questions quant à leurs éventuels effets sur la santé. La proximité de la tête avec l’antenne lors des communications, fait des cellules neuronales une cible potentielle pour les radiofréquences (RF) émises par les téléphones. L’apoptose, ou mort cellulaire programmée, est un phénomène biologique indispensable à l’organisme. C’est un mécanisme de protection contre les agressions, hautement régulé. Toutefois, une dérégulation de l’apoptose peut être à l’origine de nombreuses pathologies comme les maladies neurodégénératives ou les cancers. L’objectif de ce travail a été d’étudier l’effet des RF sur l’apoptose neuronale in vitro. Pour cela, des cellules neuronales humaines SH-SY5Y, issues d’une lignée de neuroblastomes, et des neurones corticaux de rat ont été exposés dans une cellule fil-plaque, à 900 MHz en ondes continues (CW) ou pulsées (de type GSM) pendant 24 heures, avec un DAS (débit d’absorption spécifique) maximum de 2 W/kg. L’apoptose a été étudiée immédiatement et 24 h après exposition. Une élévation de température de 2°C a été détectée dans les cultures exposées en CW. Les résultats obtenus montrent que le taux d’apoptose des cellules SH-SY5Y exposées aux RF n’augmente pas, quel que soit le type d’onde utilisé. En revanche, pour les neurones corticaux de rat, les résultats diffèrent selon le type d’onde : le taux d’apoptose des neurones exposés en GSM est comparable aux taux des témoins, alors que l’exposition en CW provoque une augmentation de l’apoptose. Cette augmentation est indépendante de l’élévation de température. La libération et la translocation dans le noyau de l’AIF (apoptosis-inducing factor) ont été observées après exposition en CW. Ces résultats indiquent que l’apoptose induite par les CW passe par la voie mitochondriale caspase-indépendante. Des études ultérieures seront nécessaires pour étudier les interactions ondes électromagnétiques/ cellules au niveau moléculaire et étudier in vivo l’effet des RF sur l’apoptose neuronale.The multiplication and the development of wireless communication give rise to many questions concerning their possible effects on health. The phone being close to the head when used, brain cells represent a major potential target for radiofrequency (RF) emitted by the phones. Apoptosis, or programmed cell death, is an important biological phenomenon for organisms. It is a major mechanism of protection against injury, highly regulated. Therefore, a dysregulation of apoptosis may be involved in different pathologies such as neurodegenerative diseases or cancers. The objective of this work was to investigate whether RF may induce neuronal apoptosis in vitro. Human neuroblastoma cell line SH-SY5Y and rat cortical neurons were exposed to 900 MHz continuous waves (CW) or to GSM-900 signal at a maximal specific absorption rate (SAR) of 2 W/kg for 24 h in a wire-patch cell. Apoptosis rate was assessed immediately and 24 h after exposure. A maximum increase of 2°C was detected in cultures exposed to CW. Results showed that under the conditions of the experiment, RF-exposure (either CW or GSM) did not significantly increase the apoptosis rate in the human neuroblastoma cell line SH-SY5Y. In contrast, in rat cortical neurons, results were different according to the wave type: the apoptosis rate of neurons exposed to GSM was similar to the rate of controls, while exposure to CW induced an increase of apoptosis. This increase was independent of the increase of temperature. The liberation and the translocation in nucleus of AIF (Apoptosis-Inducing Factor) were observed after exposure to CW. These results showed that CW induced apoptosis via a caspase-independent mitochondrial pathway. Further studies are needed to state precisely the interactions between electromagnetic waves and cells at the molecular level and to investigate the effects of the RF on the neuronal apoptosis in vivo.", "thesisType": "Thèse de doctorat", "university": "[s.n.]", "place": "[S.l.]", "date": "2006", "series": "", "seriesNumber": "", "numPages": "100", "DOI": "", "ISBN": "", "citationKey": "", "url": "", "accessDate": "", "ISSN": "", "archive": "", "archiveLocation": "", "shortTitle": "Exposition in vitro de cellules neuronales aux radiofréquences", "language": "français", "libraryCatalog": "", "callNumber": "", "rights": "", "extra": "", "tags": [ { "tag": "Apoptose -- Thèses et écrits académiques", "type": 1 }, { "tag": "Cellules -- Lignée -- Thèses et écrits académiques", "type": 1 }, { "tag": "Radiofréquences -- Dissertations académiques", "type": 1 }, { "tag": "Rats de laboratoire -- Neurones -- Thèses et écrits académiques", "type": 1 } ], "collections": [ "AJTDUI26" ], "relations": 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